Mutagenetix > Incidental Mutation

Incidental Mutation 'R6106:Morn3'

485506

Institutional Source

Beutler Lab

Gene Symbol

Morn3

Ensembl Gene

ENSMUSG00000029477

Gene Name

MORN repeat containing 3

Synonyms

4930438O03Rik

MMRRC Submission

044256-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.125) question?

Stock #

R6106 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

123173832-123185079 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 123184823 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 6 (C6R)

Ref Sequence

ENSEMBL: ENSMUSP00000120199 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031437] [ENSMUST00000045843] [ENSMUST00000132775] [ENSMUST00000145257]

AlphaFold

Q8C5T4

Predicted Effect

probably benign
Transcript: ENSMUST00000031437
AA Change: C6R

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000031437
Gene: ENSMUSG00000029477
AA Change: C6R

Domain	Start	End	E-Value	Type
MORN	36	57	3.24e-4	SMART
MORN	60	81	5.18e-3	SMART
MORN	89	110	3.51e0	SMART
MORN	112	133	3.09e-1	SMART
MORN	135	156	1.15e-4	SMART
MORN	158	179	2.57e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000045843
AA Change: C6R

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000041714
Gene: ENSMUSG00000029477
AA Change: C6R

Domain	Start	End	E-Value	Type
MORN	36	57	3.24e-4	SMART
MORN	60	81	5.18e-3	SMART
MORN	89	110	3.51e0	SMART
MORN	112	133	3.09e-1	SMART
MORN	135	156	2.26e-3	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000132775
AA Change: C6R

PolyPhen 2 Score 0.827 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000120199
Gene: ENSMUSG00000029477
AA Change: C6R

Domain	Start	End	E-Value	Type
MORN	36	57	3.24e-4	SMART
MORN	60	81	5.18e-3	SMART
MORN	89	110	3.51e0	SMART
MORN	112	133	3.09e-1	SMART
MORN	135	156	2.26e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000145257
AA Change: C6R

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000122306
Gene: ENSMUSG00000029477
AA Change: C6R

Domain	Start	End	E-Value	Type
MORN	36	57	3.24e-4	SMART
MORN	60	81	5.18e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181022

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ace	A	G	11: 105,879,838 (GRCm39)	E726G	probably damaging	Het
Adgrl4	A	G	3: 151,246,622 (GRCm39)	I641V	possibly damaging	Het
Apoa5	A	T	9: 46,181,931 (GRCm39)	R336*	probably null	Het
Bfsp2	T	A	9: 103,357,023 (GRCm39)	T135S	probably benign	Het
Calhm2	A	T	19: 47,121,501 (GRCm39)	Y223N	probably damaging	Het
Ccdc158	T	C	5: 92,775,325 (GRCm39)	E960G	probably benign	Het
Ccdc80	T	C	16: 44,917,073 (GRCm39)	S610P	probably benign	Het
Cdon	T	A	9: 35,366,704 (GRCm39)	Y193*	probably null	Het
Cept1	A	T	3: 106,410,992 (GRCm39)	H400Q	probably benign	Het
Cfap144	T	C	11: 58,687,427 (GRCm39)	E66G	probably damaging	Het
Clspn	T	A	4: 126,484,434 (GRCm39)	N1197K	probably benign	Het
Cnot8	T	C	11: 58,004,816 (GRCm39)	S172P	probably damaging	Het
Col14a1	T	C	15: 55,383,404 (GRCm39)	I1794T	probably damaging	Het
Cracdl	T	C	1: 37,652,493 (GRCm39)	T1105A	possibly damaging	Het
Fam193a	A	T	5: 34,616,374 (GRCm39)	T564S	possibly damaging	Het
Galnt1	G	A	18: 24,387,720 (GRCm39)	V154I	probably benign	Het
Gstcd	C	A	3: 132,704,675 (GRCm39)	E526D	probably benign	Het
Ighv1-42	A	C	12: 114,900,907 (GRCm39)	S59R	probably benign	Het
Nrdc	A	T	4: 108,901,782 (GRCm39)	K617M	probably damaging	Het
Or2aj4	A	G	16: 19,385,009 (GRCm39)	L208P	probably damaging	Het
Or51ai2	A	G	7: 103,587,400 (GRCm39)	H271R	probably benign	Het
Or8b36	T	A	9: 37,937,762 (GRCm39)	I220N	probably damaging	Het
Or8b49	T	C	9: 38,506,252 (GRCm39)	M245T	probably benign	Het
Pcdhb11	A	G	18: 37,556,056 (GRCm39)	N462S	probably damaging	Het
Pfpl	G	T	19: 12,406,825 (GRCm39)	D359Y	probably damaging	Het
Phyhip	T	C	14: 70,699,299 (GRCm39)	V34A	probably benign	Het
Pigu	A	T	2: 155,139,116 (GRCm39)	I313N	possibly damaging	Het
Plch1	C	T	3: 63,609,444 (GRCm39)	R912H	probably damaging	Het
Psg16	T	A	7: 16,829,091 (GRCm39)	F225Y	possibly damaging	Het
Setdb2	T	A	14: 59,660,898 (GRCm39)	K82*	probably null	Het
Sgms1	A	G	19: 32,101,825 (GRCm39)	S394P	possibly damaging	Het
Slc16a1	T	C	3: 104,560,310 (GRCm39)	L205P	probably benign	Het
Slc19a1	T	A	10: 76,880,603 (GRCm39)	I380N	probably damaging	Het
Snx32	A	G	19: 5,548,042 (GRCm39)	I131T	probably benign	Het
Sorbs3	T	C	14: 70,430,053 (GRCm39)		probably null	Het
Stc2	T	A	11: 31,310,392 (GRCm39)	I215L	probably benign	Het
Tln2	C	T	9: 67,230,302 (GRCm39)	A84T	probably damaging	Het
Tomm34	A	G	2: 163,902,911 (GRCm39)	M133T	probably benign	Het
Usp43	A	G	11: 67,770,733 (GRCm39)	S634P	probably benign	Het
Vmn2r59	T	A	7: 41,661,749 (GRCm39)	R689*	probably null	Het
Vmn2r9	C	T	5: 108,992,902 (GRCm39)	R536Q	probably benign	Het
Wdr24	C	T	17: 26,043,579 (GRCm39)	H134Y	probably benign	Het
Zfhx2	T	A	14: 55,305,767 (GRCm39)		probably null	Het
Zfp1004	A	G	2: 150,034,725 (GRCm39)	K349E	probably damaging	Het
Zfp608	G	T	18: 55,120,944 (GRCm39)	H214Q	possibly damaging	Het
Zfp619	T	C	7: 39,184,558 (GRCm39)	V196A	probably benign	Het

Other mutations in Morn3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01925:Morn3	APN	5	123,184,825 (GRCm39)	missense	probably damaging	1.00
IGL02257:Morn3	APN	5	123,175,788 (GRCm39)	missense	probably damaging	1.00
IGL02516:Morn3	APN	5	123,175,363 (GRCm39)	nonsense	probably null
R0052:Morn3	UTSW	5	123,184,726 (GRCm39)	missense	probably damaging	1.00
R0052:Morn3	UTSW	5	123,184,726 (GRCm39)	missense	probably damaging	1.00
R0433:Morn3	UTSW	5	123,177,396 (GRCm39)	missense	probably benign	0.01
R1854:Morn3	UTSW	5	123,184,692 (GRCm39)	critical splice donor site	probably null
R4893:Morn3	UTSW	5	123,175,745 (GRCm39)	missense	probably damaging	1.00
R5854:Morn3	UTSW	5	123,179,178 (GRCm39)	missense	probably damaging	1.00
R5899:Morn3	UTSW	5	123,179,166 (GRCm39)	missense	probably damaging	1.00
R5983:Morn3	UTSW	5	123,175,851 (GRCm39)	missense	possibly damaging	0.80
R6766:Morn3	UTSW	5	123,179,270 (GRCm39)	missense	probably damaging	0.98
R7512:Morn3	UTSW	5	123,175,343 (GRCm39)	critical splice donor site	probably null
R8722:Morn3	UTSW	5	123,179,177 (GRCm39)	missense	probably damaging	1.00
R8780:Morn3	UTSW	5	123,175,825 (GRCm39)	missense	probably damaging	1.00
R9782:Morn3	UTSW	5	123,175,822 (GRCm39)	missense	probably damaging	1.00
Z1177:Morn3	UTSW	5	123,184,783 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- TCACTAAGTGGGGTTGGGAAC -3'
(R):5'- CTGGGGACTCAGAACACAAATG -3'

Sequencing Primer
(F):5'- CTTAATGGACTCTGTAACAGTGC -3'
(R):5'- ATGACCTGCCCTATTGGAACTCAG -3'

Posted On

2017-08-16