Mutagenetix > Incidental Mutation

Incidental Mutation 'R6119:Zfp677'

485685

Institutional Source

Beutler Lab

Gene Symbol

Zfp677

Ensembl Gene

ENSMUSG00000062743

Gene Name

zinc finger protein 677

Synonyms

A830058L05Rik

MMRRC Submission

044429-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R6119 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21604010-21619527 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 21618070 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 376 (T376A)

Ref Sequence

ENSEMBL: ENSMUSP00000125295 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056107] [ENSMUST00000162659]

AlphaFold

Q6PEP4

Predicted Effect

possibly damaging
Transcript: ENSMUST00000056107
AA Change: T376A

PolyPhen 2 Score 0.551 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000052667
Gene: ENSMUSG00000062743
AA Change: T376A

Domain	Start	End	E-Value	Type
KRAB	13	75	1.11e-21	SMART
ZnF_C2H2	185	207	2.95e-3	SMART
ZnF_C2H2	213	235	3.95e-4	SMART
ZnF_C2H2	241	263	2.09e-3	SMART
ZnF_C2H2	269	291	6.42e-4	SMART
ZnF_C2H2	297	319	5.5e-3	SMART
ZnF_C2H2	325	347	1.98e-4	SMART
ZnF_C2H2	353	375	1.98e-4	SMART
ZnF_C2H2	381	403	1.47e-3	SMART
ZnF_C2H2	409	431	1.28e-3	SMART
ZnF_C2H2	437	459	3.95e-4	SMART
ZnF_C2H2	465	487	1.04e-3	SMART
ZnF_C2H2	493	515	8.47e-4	SMART
ZnF_C2H2	521	543	7.49e-5	SMART
ZnF_C2H2	549	571	1.18e-2	SMART
ZnF_C2H2	577	599	6.08e-5	SMART
ZnF_C2H2	610	632	4.17e-3	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000162659
AA Change: T376A

PolyPhen 2 Score 0.551 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000125295
Gene: ENSMUSG00000062743
AA Change: T376A

Domain	Start	End	E-Value	Type
KRAB	13	75	1.11e-21	SMART
Pfam:zf-H2C2_2	118	140	2.9e-5	PFAM
ZnF_C2H2	185	207	2.95e-3	SMART
ZnF_C2H2	213	235	3.95e-4	SMART
ZnF_C2H2	241	263	2.09e-3	SMART
ZnF_C2H2	269	291	6.42e-4	SMART
ZnF_C2H2	297	319	5.5e-3	SMART
ZnF_C2H2	325	347	1.98e-4	SMART
ZnF_C2H2	353	375	1.98e-4	SMART
ZnF_C2H2	381	403	1.47e-3	SMART
ZnF_C2H2	409	431	1.28e-3	SMART
ZnF_C2H2	437	459	3.95e-4	SMART
ZnF_C2H2	465	487	1.04e-3	SMART
ZnF_C2H2	493	515	8.47e-4	SMART
ZnF_C2H2	521	543	7.49e-5	SMART
ZnF_C2H2	549	571	1.18e-2	SMART
ZnF_C2H2	577	599	6.08e-5	SMART
ZnF_C2H2	610	632	4.17e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000232365

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 93.2%

Validation Efficiency

100% (53/53)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022A10Rik	C	T	7: 27,265,138 (GRCm39)	R97*	probably null	Het
4930544D05Rik	G	A	11: 70,507,317 (GRCm39)	A121T	probably damaging	Het
Abtb2	A	T	2: 103,532,655 (GRCm39)	E484D	probably benign	Het
Ankk1	A	T	9: 49,338,183 (GRCm39)	W37R	possibly damaging	Het
Arhgap11a	C	A	2: 113,664,695 (GRCm39)	M529I	probably benign	Het
Btd	G	A	14: 31,363,065 (GRCm39)		probably benign	Het
Ddx47	T	C	6: 135,000,318 (GRCm39)	I438T	probably benign	Het
Dnhd1	A	G	7: 105,358,647 (GRCm39)	T3379A	probably benign	Het
Dusp3	A	G	11: 101,871,495 (GRCm39)		probably benign	Het
Dync1h1	A	G	12: 110,594,440 (GRCm39)	K1289E	possibly damaging	Het
Egf	A	T	3: 129,530,421 (GRCm39)	I247N	probably benign	Het
Erich1	A	G	8: 14,083,692 (GRCm39)	L126P	probably benign	Het
Fat3	T	A	9: 16,287,864 (GRCm39)	H553L	possibly damaging	Het
Gdpgp1	T	C	7: 79,888,740 (GRCm39)	L257P	probably damaging	Het
Gimap8	T	C	6: 48,635,888 (GRCm39)	I551T	possibly damaging	Het
Gnptab	C	A	10: 88,267,257 (GRCm39)	D449E	probably damaging	Het
Grb10	T	C	11: 11,883,551 (GRCm39)	D513G	probably damaging	Het
Grin1	T	G	2: 25,195,170 (GRCm39)	D283A	probably damaging	Het
Gtf2i	A	T	5: 134,315,911 (GRCm39)		probably null	Het
Ip6k3	A	G	17: 27,367,599 (GRCm39)	V199A	possibly damaging	Het
Kif17	T	A	4: 138,015,643 (GRCm39)	Y405*	probably null	Het
Lair1	T	C	7: 4,031,895 (GRCm39)	M71V	probably benign	Het
Mamdc2	G	A	19: 23,330,679 (GRCm39)	T376M	probably damaging	Het
Msra	G	A	14: 64,678,183 (GRCm39)	R38C	probably damaging	Het
Mthfd1	A	T	12: 76,350,447 (GRCm39)	I462F	probably damaging	Het
Nbr1	A	G	11: 101,457,938 (GRCm39)		probably null	Het
Neb	A	G	2: 52,110,943 (GRCm39)	M181T	probably benign	Het
Noxo1	A	G	17: 24,915,545 (GRCm39)		probably benign	Het
Optn	C	A	2: 5,026,134 (GRCm39)		probably null	Het
Or10ag58	A	T	2: 87,265,733 (GRCm39)	K301*	probably null	Het
Or4c103	A	G	2: 88,513,935 (GRCm39)	I47T	probably damaging	Het
Or5k17	A	T	16: 58,746,895 (GRCm39)	I13N	possibly damaging	Het
Or6c215	G	A	10: 129,637,689 (GRCm39)	A235V	probably damaging	Het
Or6c215	C	A	10: 129,637,690 (GRCm39)	A235S	probably damaging	Het
Pclo	A	G	5: 14,727,033 (GRCm39)		probably benign	Het
Ppp1r16b	A	G	2: 158,593,047 (GRCm39)	I209V	probably benign	Het
Prss12	A	G	3: 123,283,258 (GRCm39)	I517V	possibly damaging	Het
Pwwp3a	A	G	10: 80,064,865 (GRCm39)	K32E	probably benign	Het
Ripor2	C	T	13: 24,798,627 (GRCm39)		probably benign	Het
Rmnd5b	A	G	11: 51,516,536 (GRCm39)	S274P	probably benign	Het
Sftpa1	T	A	14: 40,854,509 (GRCm39)	I32N	probably damaging	Het
Slc2a12	T	A	10: 22,541,246 (GRCm39)	I367N	probably damaging	Het
Sorcs1	T	C	19: 50,276,532 (GRCm39)	D340G	probably damaging	Het
Synj1	T	C	16: 90,735,877 (GRCm39)	K1359E	probably benign	Het
Tasor2	C	T	13: 3,631,891 (GRCm39)	R870H	possibly damaging	Het
Tcf12	T	A	9: 71,775,547 (GRCm39)	E421V	probably damaging	Het
Tecta	A	T	9: 42,284,371 (GRCm39)	F905I	probably benign	Het
Tmem106a	T	A	11: 101,474,576 (GRCm39)	C58*	probably null	Het
Tmem131l	A	T	3: 83,805,689 (GRCm39)	F1585I	probably damaging	Het
Tnks2	T	C	19: 36,856,752 (GRCm39)	S208P	possibly damaging	Het
Trim50	A	G	5: 135,382,274 (GRCm39)	N42S	probably benign	Het
Tsc22d2	T	A	3: 58,367,674 (GRCm39)		probably benign	Het
Ttll3	T	A	6: 113,371,702 (GRCm39)	L23H	probably damaging	Het
Vmn1r10	A	G	6: 57,091,218 (GRCm39)	Y270C	probably benign	Het
Ypel3	T	C	7: 126,377,537 (GRCm39)	V74A	possibly damaging	Het

Other mutations in Zfp677

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00481:Zfp677	APN	17	21,617,930 (GRCm39)	missense	probably benign	0.33
IGL01973:Zfp677	APN	17	21,617,169 (GRCm39)	missense	probably damaging	1.00
IGL02206:Zfp677	APN	17	21,613,499 (GRCm39)	missense	probably damaging	1.00
IGL03240:Zfp677	APN	17	21,617,135 (GRCm39)	missense	probably damaging	0.99
IGL03409:Zfp677	APN	17	21,617,107 (GRCm39)	missense	probably damaging	1.00
R0622:Zfp677	UTSW	17	21,617,962 (GRCm39)	missense	probably benign	0.04
R0972:Zfp677	UTSW	17	21,618,572 (GRCm39)	missense	probably damaging	1.00
R1519:Zfp677	UTSW	17	21,617,499 (GRCm39)	missense	possibly damaging	0.91
R2155:Zfp677	UTSW	17	21,617,970 (GRCm39)	missense	probably benign	0.01
R2316:Zfp677	UTSW	17	21,617,582 (GRCm39)	missense	probably benign	0.38
R2866:Zfp677	UTSW	17	21,617,518 (GRCm39)	nonsense	probably null
R2989:Zfp677	UTSW	17	21,617,114 (GRCm39)	missense	probably benign	0.11
R3955:Zfp677	UTSW	17	21,618,079 (GRCm39)	missense	possibly damaging	0.95
R4075:Zfp677	UTSW	17	21,618,421 (GRCm39)	missense	probably damaging	1.00
R4134:Zfp677	UTSW	17	21,618,043 (GRCm39)	missense	probably benign	0.01
R4229:Zfp677	UTSW	17	21,618,544 (GRCm39)	missense	probably damaging	1.00
R4729:Zfp677	UTSW	17	21,617,680 (GRCm39)	missense	possibly damaging	0.51
R4843:Zfp677	UTSW	17	21,612,788 (GRCm39)	missense	probably benign	0.23
R5023:Zfp677	UTSW	17	21,618,056 (GRCm39)	missense	probably damaging	1.00
R5316:Zfp677	UTSW	17	21,617,410 (GRCm39)	missense	probably damaging	0.99
R5420:Zfp677	UTSW	17	21,618,175 (GRCm39)	missense	probably damaging	1.00
R5694:Zfp677	UTSW	17	21,618,021 (GRCm39)	missense	probably damaging	0.99
R5837:Zfp677	UTSW	17	21,617,648 (GRCm39)	missense	probably damaging	1.00
R5888:Zfp677	UTSW	17	21,618,520 (GRCm39)	missense	probably damaging	1.00
R6007:Zfp677	UTSW	17	21,617,918 (GRCm39)	missense	probably damaging	1.00
R6190:Zfp677	UTSW	17	21,617,530 (GRCm39)	missense	possibly damaging	0.91
R6518:Zfp677	UTSW	17	21,618,392 (GRCm39)	missense	probably damaging	1.00
R7198:Zfp677	UTSW	17	21,618,679 (GRCm39)	missense	probably damaging	1.00
R7391:Zfp677	UTSW	17	21,618,653 (GRCm39)	missense	possibly damaging	0.56
R7801:Zfp677	UTSW	17	21,618,277 (GRCm39)	missense	probably damaging	1.00
R7808:Zfp677	UTSW	17	21,617,647 (GRCm39)	missense	probably damaging	1.00
R8202:Zfp677	UTSW	17	21,613,535 (GRCm39)	missense	probably damaging	1.00
R8206:Zfp677	UTSW	17	21,612,717 (GRCm39)	splice site	probably null
R8885:Zfp677	UTSW	17	21,618,350 (GRCm39)	missense	probably benign
R8965:Zfp677	UTSW	17	21,617,155 (GRCm39)	missense	probably damaging	1.00
R9062:Zfp677	UTSW	17	21,612,815 (GRCm39)	critical splice donor site	probably null
R9167:Zfp677	UTSW	17	21,613,460 (GRCm39)	missense	probably damaging	1.00
R9371:Zfp677	UTSW	17	21,618,053 (GRCm39)	missense	probably damaging	1.00
R9638:Zfp677	UTSW	17	21,618,056 (GRCm39)	missense	probably damaging	1.00
R9752:Zfp677	UTSW	17	21,618,511 (GRCm39)	missense	probably damaging	1.00
RF003:Zfp677	UTSW	17	21,617,704 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTGGAGAGAAACCCTACACGT -3'
(R):5'- TTTCTCCCCAGTATGAAGTCTTTGA -3'

Sequencing Primer
(F):5'- CCTACACGTGCAATGTTTGTGACAG -3'
(R):5'- CCACAAATGTTGCACTTGTAGGG -3'

Posted On

2017-08-16