Mutagenetix > Incidental Mutation

Incidental Mutation 'R6122:Cul9'

485803

Institutional Source

Beutler Lab

Gene Symbol

Cul9

Ensembl Gene

ENSMUSG00000040327

Gene Name

cullin 9

Synonyms

Parc, 1810035I07Rik

MMRRC Submission

044269-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.198) question?

Stock #

R6122 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

46811535-46857314 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 46832854 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1363 (D1363G)

Ref Sequence

ENSEMBL: ENSMUSP00000138418 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000066026] [ENSMUST00000182485]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000066026
AA Change: D1363G

PolyPhen 2 Score 0.321 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000067736
Gene: ENSMUSG00000040327
AA Change: D1363G

Domain	Start	End	E-Value	Type
low complexity region	46	61	N/A	INTRINSIC
low complexity region	119	131	N/A	INTRINSIC
low complexity region	345	357	N/A	INTRINSIC
Pfam:Cul7	367	441	1e-35	PFAM
low complexity region	447	460	N/A	INTRINSIC
low complexity region	525	540	N/A	INTRINSIC
low complexity region	845	860	N/A	INTRINSIC
low complexity region	873	880	N/A	INTRINSIC
APC10	1166	1325	1.97e-56	SMART
low complexity region	1437	1450	N/A	INTRINSIC
low complexity region	1563	1578	N/A	INTRINSIC
low complexity region	1646	1671	N/A	INTRINSIC
Cullin_Nedd8	1867	1950	7.55e-6	SMART
Blast:RING	2074	2122	2e-13	BLAST
IBR	2144	2207	8.99e-14	SMART
IBR	2228	2283	4.66e-2	SMART
low complexity region	2503	2520	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181301

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182315

Predicted Effect

possibly damaging
Transcript: ENSMUST00000182485
AA Change: D1363G

PolyPhen 2 Score 0.716 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000138418
Gene: ENSMUSG00000040327
AA Change: D1363G

Domain	Start	End	E-Value	Type
low complexity region	46	61	N/A	INTRINSIC
low complexity region	119	131	N/A	INTRINSIC
low complexity region	345	357	N/A	INTRINSIC
Pfam:Cul7	367	442	1.4e-33	PFAM
low complexity region	447	460	N/A	INTRINSIC
low complexity region	525	540	N/A	INTRINSIC
low complexity region	845	860	N/A	INTRINSIC
low complexity region	873	880	N/A	INTRINSIC
APC10	1166	1325	1.97e-56	SMART
low complexity region	1437	1450	N/A	INTRINSIC
low complexity region	1563	1578	N/A	INTRINSIC
low complexity region	1646	1671	N/A	INTRINSIC
Cullin_Nedd8	1867	1950	7.55e-6	SMART
Blast:RING	2074	2122	3e-13	BLAST
IBR	2144	2207	8.99e-14	SMART
IBR	2228	2283	4.66e-2	SMART
coiled coil region	2461	2497	N/A	INTRINSIC
low complexity region	2513	2530	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182530

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182668

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182799

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000183016

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000183078

Meta Mutation Damage Score

0.0825

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.8%

Validation Efficiency

98% (57/58)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit decreased body weight, increased incidence of tumors, and decreased cellular sensitivity to radiation-induced apoptosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700034J05Rik	T	C	6: 146,853,750 (GRCm39)	*301W	probably null	Het
Abat	A	G	16: 8,423,414 (GRCm39)	I236V	probably benign	Het
Abca8a	T	C	11: 109,961,249 (GRCm39)	T558A	probably benign	Het
Ace3	A	C	11: 105,885,764 (GRCm39)	M57L	probably benign	Het
Adgrg1	T	C	8: 95,729,129 (GRCm39)	S18P	probably benign	Het
Ahi1	A	G	10: 20,934,064 (GRCm39)	D60G	probably benign	Het
Ap4e1	T	A	2: 126,870,080 (GRCm39)		probably null	Het
Arnt2	C	A	7: 84,010,773 (GRCm39)	K34N	probably damaging	Het
Ascc3	T	A	10: 50,494,021 (GRCm39)	M152K	probably benign	Het
AW551984	A	T	9: 39,505,051 (GRCm39)	F480L	probably benign	Het
Blvrb	G	A	7: 27,158,773 (GRCm39)	A58T	possibly damaging	Het
Bpifa1	C	T	2: 153,985,892 (GRCm39)	T69I	probably benign	Het
Cacna1g	T	A	11: 94,320,997 (GRCm39)	M1366L	probably benign	Het
Ccdc82	A	G	9: 13,266,880 (GRCm39)	I361V	probably benign	Het
Clca4b	T	A	3: 144,631,927 (GRCm39)	I193F	possibly damaging	Het
Crb1	A	T	1: 139,176,686 (GRCm39)	Y371*	probably null	Het
Cyp2j8	C	T	4: 96,332,877 (GRCm39)	A490T	probably benign	Het
D430041D05Rik	A	T	2: 104,086,637 (GRCm39)	S780T	probably benign	Het
Fzd5	G	T	1: 64,774,815 (GRCm39)	C315*	probably null	Het
Gabrr1	T	C	4: 33,161,695 (GRCm39)	S340P	probably damaging	Het
Galnt7	T	C	8: 57,979,200 (GRCm39)	D641G	probably damaging	Het
Gm7133	A	G	1: 97,110,948 (GRCm39)		noncoding transcript	Het
Heatr5b	A	G	17: 79,120,602 (GRCm39)	L774P	possibly damaging	Het
Itch	T	A	2: 155,015,985 (GRCm39)	S157T	probably benign	Het
Itgam	A	C	7: 127,684,824 (GRCm39)	D398A	probably damaging	Het
Kalrn	G	T	16: 33,805,561 (GRCm39)	L2659M	possibly damaging	Het
Kcnj14	C	A	7: 45,468,875 (GRCm39)	R210L	possibly damaging	Het
Kmt2d	T	C	15: 98,758,573 (GRCm39)		probably benign	Het
Krt1c	G	C	15: 101,724,349 (GRCm39)	T305S	probably damaging	Het
Mcm3ap	A	G	10: 76,342,441 (GRCm39)	N1645D	probably damaging	Het
Naf1	T	A	8: 67,336,096 (GRCm39)	I341K	probably damaging	Het
Nbea	G	T	3: 55,937,317 (GRCm39)	H765N	probably damaging	Het
Ncapd3	A	G	9: 26,975,278 (GRCm39)	I776V	probably benign	Het
Neo1	A	T	9: 58,824,291 (GRCm39)	D712E	probably benign	Het
Neu1	G	A	17: 35,153,730 (GRCm39)	V385I	probably benign	Het
Or14j3	C	A	17: 37,900,817 (GRCm39)	R142S	probably benign	Het
Or51a6	C	A	7: 102,604,011 (GRCm39)	G273C	probably damaging	Het
Or51a6	A	G	7: 102,604,737 (GRCm39)	Y24H	probably benign	Het
Pgghg	A	C	7: 140,523,308 (GRCm39)	T196P	possibly damaging	Het
Pira2	A	T	7: 3,845,445 (GRCm39)	V313E	probably damaging	Het
Pkd1l2	T	C	8: 117,809,107 (GRCm39)	R28G	probably benign	Het
Pkhd1l1	T	C	15: 44,421,336 (GRCm39)	S3035P	probably damaging	Het
Ppp1r9a	T	A	6: 4,905,509 (GRCm39)	N21K	probably damaging	Het
Prune1	T	C	3: 95,169,554 (GRCm39)	D216G	probably benign	Het
Ptpn23	G	T	9: 110,216,893 (GRCm39)		probably benign	Het
Ranbp2	T	A	10: 58,301,351 (GRCm39)	M668K	probably benign	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Rpsa	G	T	9: 119,960,102 (GRCm39)	V222L	probably benign	Het
Septin2	A	G	1: 93,425,098 (GRCm39)	T45A	probably damaging	Het
Slc12a8	A	T	16: 33,445,384 (GRCm39)	D260V	probably damaging	Het
Srrm2	G	T	17: 24,039,330 (GRCm39)	M2087I	possibly damaging	Het
Tg	T	C	15: 66,700,306 (GRCm39)	L88P	probably damaging	Het
Tns1	A	G	1: 73,991,578 (GRCm39)		probably null	Het
Topbp1	A	T	9: 103,224,160 (GRCm39)	D1429V	probably benign	Het
Tprg1	A	G	16: 25,241,151 (GRCm39)		probably null	Het
Trit1	T	C	4: 122,933,261 (GRCm39)	I66T	possibly damaging	Het
Usf3	A	T	16: 44,037,670 (GRCm39)	I717L	probably damaging	Het

Other mutations in Cul9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Cul9	APN	17	46,836,635 (GRCm39)	missense	probably damaging	1.00
IGL00330:Cul9	APN	17	46,821,767 (GRCm39)	splice site	probably benign
IGL00726:Cul9	APN	17	46,839,022 (GRCm39)	missense	probably damaging	1.00
IGL01020:Cul9	APN	17	46,849,949 (GRCm39)	missense	probably damaging	1.00
IGL01358:Cul9	APN	17	46,849,240 (GRCm39)	missense	probably damaging	1.00
IGL01410:Cul9	APN	17	46,839,572 (GRCm39)	missense	probably damaging	0.99
IGL01781:Cul9	APN	17	46,850,230 (GRCm39)	missense	probably benign
IGL01873:Cul9	APN	17	46,813,378 (GRCm39)	missense	probably damaging	0.99
IGL02117:Cul9	APN	17	46,851,301 (GRCm39)	missense	probably benign	0.00
IGL02300:Cul9	APN	17	46,831,958 (GRCm39)	splice site	probably benign
IGL02426:Cul9	APN	17	46,834,184 (GRCm39)	missense	possibly damaging	0.95
IGL02427:Cul9	APN	17	46,813,558 (GRCm39)	missense	possibly damaging	0.69
IGL02496:Cul9	APN	17	46,851,302 (GRCm39)	missense	possibly damaging	0.72
IGL03008:Cul9	APN	17	46,813,623 (GRCm39)	splice site	probably benign
IGL03059:Cul9	APN	17	46,849,913 (GRCm39)	missense	probably damaging	0.98
IGL03302:Cul9	APN	17	46,837,566 (GRCm39)	missense	probably damaging	0.98
bottlenose	UTSW	17	46,811,770 (GRCm39)	missense	possibly damaging	0.79
flipper	UTSW	17	46,836,818 (GRCm39)	missense	probably benign	0.05
orca	UTSW	17	46,836,061 (GRCm39)	missense	probably damaging	1.00
FR4340:Cul9	UTSW	17	46,811,779 (GRCm39)	small insertion	probably benign
FR4449:Cul9	UTSW	17	46,811,782 (GRCm39)	small insertion	probably benign
FR4737:Cul9	UTSW	17	46,811,784 (GRCm39)	small insertion	probably benign
FR4737:Cul9	UTSW	17	46,811,772 (GRCm39)	small insertion	probably benign
FR4976:Cul9	UTSW	17	46,811,779 (GRCm39)	small insertion	probably benign
FR4976:Cul9	UTSW	17	46,811,776 (GRCm39)	small insertion	probably benign
FR4976:Cul9	UTSW	17	46,811,774 (GRCm39)	small insertion	probably benign
FR4976:Cul9	UTSW	17	46,811,782 (GRCm39)	small insertion	probably benign
R0012:Cul9	UTSW	17	46,849,436 (GRCm39)	missense	probably benign	0.26
R0079:Cul9	UTSW	17	46,848,589 (GRCm39)	nonsense	probably null
R0143:Cul9	UTSW	17	46,837,336 (GRCm39)	missense	possibly damaging	0.65
R0390:Cul9	UTSW	17	46,839,515 (GRCm39)	missense	probably benign	0.34
R0401:Cul9	UTSW	17	46,852,630 (GRCm39)	missense	probably damaging	1.00
R0529:Cul9	UTSW	17	46,831,394 (GRCm39)	splice site	probably benign
R0815:Cul9	UTSW	17	46,848,748 (GRCm39)	splice site	probably null
R0863:Cul9	UTSW	17	46,848,748 (GRCm39)	splice site	probably null
R0972:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R1173:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R1216:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R1217:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R1261:Cul9	UTSW	17	46,836,708 (GRCm39)	missense	probably damaging	1.00
R1278:Cul9	UTSW	17	46,811,775 (GRCm39)	small deletion	probably benign
R1281:Cul9	UTSW	17	46,822,460 (GRCm39)	missense	probably damaging	1.00
R1349:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R1372:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R1403:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R1403:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R1405:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R1405:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R1467:Cul9	UTSW	17	46,836,299 (GRCm39)	missense	probably damaging	1.00
R1467:Cul9	UTSW	17	46,836,299 (GRCm39)	missense	probably damaging	1.00
R1482:Cul9	UTSW	17	46,819,473 (GRCm39)	missense	probably damaging	0.99
R1491:Cul9	UTSW	17	46,849,490 (GRCm39)	nonsense	probably null
R1618:Cul9	UTSW	17	46,836,818 (GRCm39)	missense	probably benign	0.05
R1641:Cul9	UTSW	17	46,854,486 (GRCm39)	missense	possibly damaging	0.96
R1679:Cul9	UTSW	17	46,832,082 (GRCm39)	missense	possibly damaging	0.90
R1771:Cul9	UTSW	17	46,848,738 (GRCm39)	missense	probably benign	0.41
R1803:Cul9	UTSW	17	46,814,023 (GRCm39)	missense	probably damaging	1.00
R2020:Cul9	UTSW	17	46,833,101 (GRCm39)	missense	probably damaging	1.00
R2046:Cul9	UTSW	17	46,854,659 (GRCm39)	missense	probably damaging	1.00
R2056:Cul9	UTSW	17	46,854,298 (GRCm39)	missense	probably benign
R2088:Cul9	UTSW	17	46,837,575 (GRCm39)	missense	probably damaging	1.00
R2415:Cul9	UTSW	17	46,854,364 (GRCm39)	missense	probably benign
R2925:Cul9	UTSW	17	46,821,907 (GRCm39)	missense	probably benign	0.08
R2964:Cul9	UTSW	17	46,813,154 (GRCm39)	missense	probably damaging	0.96
R2965:Cul9	UTSW	17	46,813,154 (GRCm39)	missense	probably damaging	0.96
R3690:Cul9	UTSW	17	46,814,957 (GRCm39)	splice site	probably null
R3847:Cul9	UTSW	17	46,836,061 (GRCm39)	missense	probably damaging	1.00
R4437:Cul9	UTSW	17	46,813,085 (GRCm39)	missense	probably damaging	1.00
R4470:Cul9	UTSW	17	46,849,262 (GRCm39)	missense	probably benign	0.00
R4540:Cul9	UTSW	17	46,814,015 (GRCm39)	missense	probably null	0.98
R4555:Cul9	UTSW	17	46,812,755 (GRCm39)	missense	possibly damaging	0.82
R4604:Cul9	UTSW	17	46,841,072 (GRCm39)	missense	probably damaging	0.99
R4646:Cul9	UTSW	17	46,849,943 (GRCm39)	nonsense	probably null
R4799:Cul9	UTSW	17	46,811,770 (GRCm39)	missense	possibly damaging	0.79
R4822:Cul9	UTSW	17	46,840,977 (GRCm39)	missense	probably benign	0.01
R4964:Cul9	UTSW	17	46,849,451 (GRCm39)	missense	probably damaging	1.00
R4965:Cul9	UTSW	17	46,849,451 (GRCm39)	missense	probably damaging	1.00
R5027:Cul9	UTSW	17	46,811,708 (GRCm39)	missense	probably damaging	0.99
R5185:Cul9	UTSW	17	46,836,758 (GRCm39)	missense	possibly damaging	0.95
R5237:Cul9	UTSW	17	46,854,393 (GRCm39)	missense	probably benign	0.00
R5278:Cul9	UTSW	17	46,821,799 (GRCm39)	missense	probably damaging	1.00
R5361:Cul9	UTSW	17	46,811,775 (GRCm39)	small deletion	probably benign
R5455:Cul9	UTSW	17	46,821,772 (GRCm39)	splice site	probably null
R5592:Cul9	UTSW	17	46,831,517 (GRCm39)	missense	probably benign	0.00
R5597:Cul9	UTSW	17	46,813,591 (GRCm39)	missense	possibly damaging	0.56
R5613:Cul9	UTSW	17	46,814,770 (GRCm39)	missense	probably damaging	1.00
R6135:Cul9	UTSW	17	46,832,379 (GRCm39)	missense	probably benign
R6352:Cul9	UTSW	17	46,822,241 (GRCm39)	missense	probably benign	0.00
R6376:Cul9	UTSW	17	46,819,489 (GRCm39)	missense	probably damaging	1.00
R6868:Cul9	UTSW	17	46,833,109 (GRCm39)	missense	possibly damaging	0.73
R6898:Cul9	UTSW	17	46,821,952 (GRCm39)	missense	possibly damaging	0.87
R7090:Cul9	UTSW	17	46,811,765 (GRCm39)	missense	probably damaging	0.96
R7193:Cul9	UTSW	17	46,849,423 (GRCm39)	missense	probably damaging	0.98
R7221:Cul9	UTSW	17	46,839,491 (GRCm39)	missense	probably damaging	0.99
R7291:Cul9	UTSW	17	46,851,359 (GRCm39)	missense	probably benign	0.00
R7320:Cul9	UTSW	17	46,821,835 (GRCm39)	missense	possibly damaging	0.80
R7348:Cul9	UTSW	17	46,821,919 (GRCm39)	missense	possibly damaging	0.89
R7463:Cul9	UTSW	17	46,831,402 (GRCm39)	splice site	probably null
R7480:Cul9	UTSW	17	46,848,738 (GRCm39)	missense	probably benign	0.41
R7573:Cul9	UTSW	17	46,830,836 (GRCm39)	missense	probably benign
R7582:Cul9	UTSW	17	46,821,905 (GRCm39)	missense	probably damaging	1.00
R7605:Cul9	UTSW	17	46,852,658 (GRCm39)	missense	probably damaging	0.99
R7684:Cul9	UTSW	17	46,820,815 (GRCm39)	missense	probably damaging	1.00
R7830:Cul9	UTSW	17	46,851,237 (GRCm39)	missense	probably benign	0.37
R7834:Cul9	UTSW	17	46,836,630 (GRCm39)	splice site	probably null
R8131:Cul9	UTSW	17	46,822,168 (GRCm39)	missense	probably damaging	1.00
R8192:Cul9	UTSW	17	46,849,273 (GRCm39)	missense	probably benign	0.01
R8231:Cul9	UTSW	17	46,831,427 (GRCm39)	missense	probably damaging	0.99
R8248:Cul9	UTSW	17	46,840,940 (GRCm39)	missense	probably damaging	0.99
R8504:Cul9	UTSW	17	46,814,506 (GRCm39)	missense	probably damaging	1.00
R8550:Cul9	UTSW	17	46,830,772 (GRCm39)	missense	probably damaging	1.00
R8716:Cul9	UTSW	17	46,838,840 (GRCm39)	missense	probably benign	0.28
R8769:Cul9	UTSW	17	46,832,828 (GRCm39)	missense	possibly damaging	0.85
R8893:Cul9	UTSW	17	46,811,775 (GRCm39)	small deletion	probably benign
R8904:Cul9	UTSW	17	46,831,427 (GRCm39)	missense	probably damaging	0.99
R8936:Cul9	UTSW	17	46,839,528 (GRCm39)	missense	possibly damaging	0.82
R8972:Cul9	UTSW	17	46,854,177 (GRCm39)	missense	probably damaging	1.00
R9003:Cul9	UTSW	17	46,836,001 (GRCm39)	missense	possibly damaging	0.78
R9012:Cul9	UTSW	17	46,854,447 (GRCm39)	missense	probably benign
R9056:Cul9	UTSW	17	46,854,696 (GRCm39)	missense	probably damaging	0.99
R9071:Cul9	UTSW	17	46,837,379 (GRCm39)	missense	probably benign
R9162:Cul9	UTSW	17	46,837,529 (GRCm39)	missense	probably benign	0.32
R9476:Cul9	UTSW	17	46,821,833 (GRCm39)	missense	probably damaging	1.00
R9526:Cul9	UTSW	17	46,841,026 (GRCm39)	missense	probably benign	0.41
R9563:Cul9	UTSW	17	46,820,897 (GRCm39)	missense	probably benign	0.01
R9568:Cul9	UTSW	17	46,831,044 (GRCm39)	missense	possibly damaging	0.56
R9610:Cul9	UTSW	17	46,830,823 (GRCm39)	missense	possibly damaging	0.96
R9611:Cul9	UTSW	17	46,830,823 (GRCm39)	missense	possibly damaging	0.96
R9705:Cul9	UTSW	17	46,854,226 (GRCm39)	missense	probably damaging	1.00
R9765:Cul9	UTSW	17	46,850,224 (GRCm39)	missense	probably benign	0.18
RF011:Cul9	UTSW	17	46,811,774 (GRCm39)	small insertion	probably benign
RF016:Cul9	UTSW	17	46,811,789 (GRCm39)	nonsense	probably null
RF026:Cul9	UTSW	17	46,811,795 (GRCm39)	nonsense	probably null
RF027:Cul9	UTSW	17	46,811,774 (GRCm39)	small insertion	probably benign
RF030:Cul9	UTSW	17	46,811,795 (GRCm39)	small insertion	probably benign
RF033:Cul9	UTSW	17	46,811,780 (GRCm39)	small insertion	probably benign
RF039:Cul9	UTSW	17	46,811,780 (GRCm39)	small insertion	probably benign
RF041:Cul9	UTSW	17	46,811,780 (GRCm39)	nonsense	probably null
RF042:Cul9	UTSW	17	46,851,541 (GRCm39)	frame shift	probably null
RF057:Cul9	UTSW	17	46,811,789 (GRCm39)	nonsense	probably null
Z1176:Cul9	UTSW	17	46,831,511 (GRCm39)	nonsense	probably null
Z1176:Cul9	UTSW	17	46,831,502 (GRCm39)	nonsense	probably null
Z1177:Cul9	UTSW	17	46,848,723 (GRCm39)	missense	probably benign	0.14

Predicted Primers

PCR Primer
(F):5'- CATCGAAGAGTGAACAGGCC -3'
(R):5'- TCTATCCGAGGTGGGTACAG -3'

Sequencing Primer
(F):5'- GCCAATGAGGGGACAAATCC -3'
(R):5'- TACAGCTCAGGAGGGACC -3'

Posted On

2017-08-16