Incidental Mutation 'R6039:Cdkn3'
ID 486761
Institutional Source Beutler Lab
Gene Symbol Cdkn3
Ensembl Gene ENSMUSG00000037628
Gene Name cyclin dependent kinase inhibitor 3
Synonyms 2410006H10Rik, KAP
MMRRC Submission 043259-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6039 (G1)
Quality Score 225.009
Status Not validated
Chromosome 14
Chromosomal Location 46997912-47008987 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 47007373 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glycine to Arginine at position 177 (G177R)
Ref Sequence ENSEMBL: ENSMUSP00000070575 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000067426] [ENSMUST00000227149] [ENSMUST00000228106]
AlphaFold Q810P3
Predicted Effect probably damaging
Transcript: ENSMUST00000067426
AA Change: G177R

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000070575
Gene: ENSMUSG00000037628
AA Change: G177R

DomainStartEndE-ValueType
PTPc_DSPc 7 197 2.8e-3 SMART
low complexity region 199 210 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000226621
Predicted Effect probably benign
Transcript: ENSMUST00000227149
Predicted Effect probably benign
Transcript: ENSMUST00000228106
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.1%
  • 20x: 94.5%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Allele List at MGI

All alleles(2) : Targeted(2)
Other mutations in this stock
Total: 55 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adamts9 C T 6: 92,885,527 (GRCm39) R416Q possibly damaging Het
Adgre1 C A 17: 57,713,859 (GRCm39) D181E probably benign Het
Arhgap24 T C 5: 103,028,652 (GRCm39) S183P probably damaging Het
Asb1 T C 1: 91,474,748 (GRCm39) L90P probably damaging Het
Bmp3 T C 5: 99,020,209 (GRCm39) F211L probably benign Het
C8a A G 4: 104,703,139 (GRCm39) I306T probably benign Het
Chat A G 14: 32,170,984 (GRCm39) S172P probably damaging Het
Chd5 CAAGAAGAAGAAGAAGAA CAAGAAGAAGAAGAA 4: 152,438,078 (GRCm39) probably benign Het
Cyp4a12a T C 4: 115,184,420 (GRCm39) I265T probably damaging Het
Dlst T A 12: 85,165,664 (GRCm39) probably null Het
Dnmt1 A T 9: 20,837,716 (GRCm39) probably benign Het
Fbn1 C T 2: 125,205,800 (GRCm39) E1066K probably damaging Het
Fetub C T 16: 22,751,081 (GRCm39) R143C probably damaging Het
Fstl1 T A 16: 37,641,538 (GRCm39) S105T probably benign Het
Gm19410 C G 8: 36,276,518 (GRCm39) A1725G probably benign Het
Hspb7 A G 4: 141,151,215 (GRCm39) N119S probably damaging Het
Il20 T A 1: 130,838,478 (GRCm39) D73V possibly damaging Het
Insrr T C 3: 87,716,608 (GRCm39) I612T possibly damaging Het
Kcnma1 T C 14: 23,359,105 (GRCm39) Y1107C probably benign Het
Klhl32 A G 4: 24,792,615 (GRCm39) probably null Het
Krt77 C A 15: 101,769,351 (GRCm39) R470L possibly damaging Het
Muc1 T C 3: 89,139,336 (GRCm39) Y579H probably damaging Het
Myof T C 19: 37,966,132 (GRCm39) D396G probably damaging Het
Myom1 T G 17: 71,417,746 (GRCm39) D1316E probably damaging Het
Nbea T C 3: 55,912,538 (GRCm39) E1083G probably benign Het
Nek9 G A 12: 85,359,859 (GRCm39) A531V probably benign Het
Nlrp12 A T 7: 3,290,002 (GRCm39) I170N possibly damaging Het
Nup98 A G 7: 101,784,002 (GRCm39) I1093T probably benign Het
Ovch2 G A 7: 107,388,318 (GRCm39) L421F probably damaging Het
Paqr4 T C 17: 23,958,968 (GRCm39) D11G probably damaging Het
Parp8 A G 13: 117,014,134 (GRCm39) S561P probably damaging Het
Pcgf5 T A 19: 36,420,306 (GRCm39) F179L probably damaging Het
Pde4d A T 13: 110,084,876 (GRCm39) Q422L probably damaging Het
Pigs T A 11: 78,232,651 (GRCm39) V495E probably damaging Het
Polh C T 17: 46,498,959 (GRCm39) R252H probably benign Het
Psg28 T C 7: 18,160,107 (GRCm39) D363G possibly damaging Het
Ptgir A T 7: 16,640,815 (GRCm39) I36F possibly damaging Het
Ptprr G A 10: 116,072,668 (GRCm39) probably null Het
Rax C T 18: 66,068,418 (GRCm39) G229D unknown Het
Secisbp2l T A 2: 125,615,136 (GRCm39) H163L probably benign Het
Sf3a2 A G 10: 80,637,297 (GRCm39) Y45C probably damaging Het
Sgo2a C T 1: 58,055,775 (GRCm39) T653I possibly damaging Het
Slc38a9 A T 13: 112,806,231 (GRCm39) I26F probably damaging Het
Slc5a8 A G 10: 88,722,436 (GRCm39) I90V probably benign Het
Slx4 G A 16: 3,803,911 (GRCm39) H968Y possibly damaging Het
Stam2 T C 2: 52,599,611 (GRCm39) T233A probably benign Het
Tln1 G C 4: 43,555,052 (GRCm39) F259L probably damaging Het
Trav4-2 G A 14: 53,655,866 (GRCm39) V8M possibly damaging Het
Ttll5 T A 12: 85,878,729 (GRCm39) H45Q probably damaging Het
Ttn C T 2: 76,714,762 (GRCm39) probably benign Het
Ush2a T A 1: 188,051,217 (GRCm39) F234L possibly damaging Het
Vmn2r74 T A 7: 85,607,526 (GRCm39) probably null Het
Wdr6 CTG C 9: 108,450,994 (GRCm39) probably null Het
Zfp644 A G 5: 106,783,291 (GRCm39) S997P possibly damaging Het
Zfp853 G A 5: 143,274,529 (GRCm39) Q364* probably null Het
Other mutations in Cdkn3
AlleleSourceChrCoordTypePredicted EffectPPH Score
E0374:Cdkn3 UTSW 14 47,004,630 (GRCm39) splice site probably null
R0033:Cdkn3 UTSW 14 47,006,329 (GRCm39) nonsense probably null
R0033:Cdkn3 UTSW 14 47,006,329 (GRCm39) nonsense probably null
R0445:Cdkn3 UTSW 14 47,004,857 (GRCm39) critical splice donor site probably null
R1912:Cdkn3 UTSW 14 47,007,291 (GRCm39) critical splice acceptor site probably null
R3176:Cdkn3 UTSW 14 47,008,934 (GRCm39) unclassified probably benign
R3276:Cdkn3 UTSW 14 47,008,934 (GRCm39) unclassified probably benign
R4941:Cdkn3 UTSW 14 47,007,320 (GRCm39) missense possibly damaging 0.90
R5344:Cdkn3 UTSW 14 47,004,807 (GRCm39) missense possibly damaging 0.94
R5964:Cdkn3 UTSW 14 47,004,674 (GRCm39) missense probably null 1.00
R6039:Cdkn3 UTSW 14 47,007,373 (GRCm39) missense probably damaging 1.00
R7073:Cdkn3 UTSW 14 47,004,647 (GRCm39) missense possibly damaging 0.79
R7234:Cdkn3 UTSW 14 47,008,918 (GRCm39) missense unknown
R8083:Cdkn3 UTSW 14 47,000,058 (GRCm39) missense probably benign 0.06
R8314:Cdkn3 UTSW 14 47,007,330 (GRCm39) synonymous silent
R8948:Cdkn3 UTSW 14 47,004,780 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GATGGAGTTTGCTGCAACG -3'
(R):5'- GACAGAGGCTGAACAATCGC -3'

Sequencing Primer
(F):5'- GTTCTCAGTAGCTGCTTG -3'
(R):5'- CAGAGGCTGAACAATCGCAGATC -3'
Posted On 2017-08-16