Mutagenetix > Incidental Mutation

Incidental Mutation 'PIT4142001:Vldlr'

486998

Institutional Source

Beutler Lab

Gene Symbol

Vldlr

Ensembl Gene

ENSMUSG00000024924

Gene Name

very low density lipoprotein receptor

Synonyms

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.250) question?

Stock #

PIT4142001 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

27193884-27231631 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 27212269 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 94 (D94E)

Ref Sequence

ENSEMBL: ENSMUSP00000127329 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025866] [ENSMUST00000047645] [ENSMUST00000164746] [ENSMUST00000165761] [ENSMUST00000167487] [ENSMUST00000172302]

AlphaFold

P98156

Predicted Effect

probably benign
Transcript: ENSMUST00000025866
AA Change: D94E

PolyPhen 2 Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000025866
Gene: ENSMUSG00000024924
AA Change: D94E

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
EGF_like	32	68	7.38e1	SMART
LDLa	32	69	1.69e-16	SMART
LDLa	71	110	5.81e-15	SMART
LDLa	112	151	1.96e-12	SMART
LDLa	153	190	7.15e-15	SMART
LDLa	192	231	1.23e-13	SMART
LDLa	238	275	1.1e-15	SMART
LDLa	277	314	1.13e-12	SMART
LDLa	317	357	3.86e-11	SMART
EGF_CA	356	395	1e-5	SMART
EGF_CA	396	435	6.1e-10	SMART
Blast:LY	461	495	4e-15	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000047645
AA Change: D94E

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000049145
Gene: ENSMUSG00000024924
AA Change: D94E

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
EGF_like	32	68	7.38e1	SMART
LDLa	32	69	1.69e-16	SMART
LDLa	71	110	1.25e-14	SMART
LDLa	112	149	7.15e-15	SMART
LDLa	151	190	1.23e-13	SMART
LDLa	197	234	1.1e-15	SMART
LDLa	236	273	1.13e-12	SMART
LDLa	276	316	3.86e-11	SMART
EGF_CA	315	354	1e-5	SMART
EGF_CA	355	394	6.1e-10	SMART
LY	420	462	2.16e-1	SMART
LY	464	506	9.54e-12	SMART
LY	507	550	2.22e-12	SMART
LY	551	593	1.66e-11	SMART
LY	594	637	5.97e-4	SMART
EGF	664	709	2.16e-1	SMART
transmembrane domain	728	750	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000163729

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000164509

Predicted Effect

probably benign
Transcript: ENSMUST00000164746

SMART Domains

Protein: ENSMUSP00000128193
Gene: ENSMUSG00000024924

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
LDLa	32	69	1.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000165761

SMART Domains

Protein: ENSMUSP00000130382
Gene: ENSMUSG00000024924

Domain	Start	End	E-Value	Type
LDLa	1	26	1.58e0	SMART
EGF	28	64	4e-5	SMART
LY	88	130	2.16e-1	SMART
LY	132	174	9.54e-12	SMART
LY	175	218	2.22e-12	SMART
LY	219	258	3.25e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000167487
AA Change: D94E

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000127329
Gene: ENSMUSG00000024924
AA Change: D94E

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
EGF_like	32	68	7.38e1	SMART
LDLa	32	69	1.69e-16	SMART
LDLa	71	110	5.81e-15	SMART
LDLa	112	151	1.96e-12	SMART
LDLa	153	190	7.15e-15	SMART
LDLa	192	231	1.23e-13	SMART
LDLa	238	275	1.1e-15	SMART
LDLa	277	314	1.13e-12	SMART
LDLa	317	357	3.86e-11	SMART
EGF_CA	356	395	1e-5	SMART
EGF_CA	396	435	6.1e-10	SMART
LY	461	503	2.16e-1	SMART
LY	505	547	9.54e-12	SMART
LY	548	591	2.22e-12	SMART
LY	592	634	1.66e-11	SMART
LY	635	678	5.97e-4	SMART
EGF	705	750	2.16e-1	SMART
transmembrane domain	797	819	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000172302
AA Change: D94E

PolyPhen 2 Score 0.022 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000126730
Gene: ENSMUSG00000024924
AA Change: D94E

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
EGF_like	32	68	7.38e1	SMART
LDLa	32	69	1.69e-16	SMART
LDLa	71	110	5.81e-15	SMART
LDLa	112	151	1.96e-12	SMART
LDLa	153	190	7.15e-15	SMART
LDLa	192	231	1.23e-13	SMART
LDLa	238	275	1.1e-15	SMART
LDLa	277	314	1.13e-12	SMART
LDLa	317	357	3.86e-11	SMART
EGF_CA	356	395	1e-5	SMART
EGF_CA	396	435	6.1e-10	SMART
LY	461	503	2.16e-1	SMART
LY	505	547	9.54e-12	SMART
LY	548	591	2.22e-12	SMART
LY	592	634	1.66e-11	SMART
LY	635	678	5.97e-4	SMART
EGF	705	750	2.16e-1	SMART
transmembrane domain	769	791	N/A	INTRINSIC

Meta Mutation Damage Score

0.0823

Coding Region Coverage

1x: 0.0%
3x: 0.0%
10x: 0.0%
20x: 0.0%

Validation Efficiency

92% (105/114)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygous null mutants exhibit modest reductions in body weight and adiposity. In behavioral tests, mutants display deficits in contextual fear conditioning and long term potentiation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 117 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcg5	T	G	17: 84,981,022 (GRCm39)	Y107S	possibly damaging	Het
Abhd8	T	C	8: 71,914,499 (GRCm39)	E43G	probably damaging	Het
Ap1b1	T	G	11: 4,990,360 (GRCm39)	L872W	probably damaging	Het
Arid1b	T	A	17: 5,389,518 (GRCm39)	M1688K	probably damaging	Het
Brca1	T	C	11: 101,413,248 (GRCm39)		probably benign	Het
C4b	C	T	17: 34,952,675 (GRCm39)	V1151I	probably benign	Het
Card6	T	A	15: 5,128,113 (GRCm39)	Q1094H	unknown	Het
Cd22	C	A	7: 30,577,224 (GRCm39)	V28F	possibly damaging	Het
Cdc42bpa	A	T	1: 179,859,125 (GRCm39)	N109I	probably damaging	Het
Ces2b	T	C	8: 105,563,442 (GRCm39)	Y390H	probably damaging	Het
Clca4a	T	C	3: 144,674,072 (GRCm39)	Y221C	probably damaging	Het
Cntnap5a	G	T	1: 115,612,686 (GRCm39)		probably benign	Het
Cyp4a10	C	A	4: 115,382,072 (GRCm39)	H251Q	probably damaging	Het
Dcc	A	T	18: 71,517,297 (GRCm39)		probably null	Het
Dnaaf5	A	G	5: 139,171,273 (GRCm39)	K812E	possibly damaging	Het
Dnajc13	T	C	9: 104,115,672 (GRCm39)	T46A	probably damaging	Het
Duxf1	A	G	10: 58,059,276 (GRCm39)	C493R	probably benign	Het
Duxf1	C	T	10: 58,060,704 (GRCm39)	E17K	possibly damaging	Het
Duxf1	G	A	10: 58,060,136 (GRCm39)		probably benign	Het
Duxf1	G	C	10: 58,059,278 (GRCm39)	P492R	probably damaging	Het
Duxf3	C	A	10: 58,066,990 (GRCm39)	C503F	probably damaging	Het
Duxf3	C	A	10: 58,066,810 (GRCm39)	R563M	probably damaging	Het
Duxf3	A	C	10: 58,067,498 (GRCm39)	S27A	probably benign	Het
Eps8l1	G	A	7: 4,474,414 (GRCm39)	S295N	probably benign	Het
Etfdh	C	T	3: 79,517,174 (GRCm39)	S345N	probably damaging	Het
Fat3	A	G	9: 15,903,414 (GRCm39)		probably null	Het
G530012D18Rik	T	G	1: 85,504,925 (GRCm39)		probably benign	Het
Gabra4	T	C	5: 71,729,106 (GRCm39)	N558S	probably damaging	Het
Gm10718	A	T	9: 3,024,417 (GRCm39)	T134S	probably benign	Het
Gm10722	T	C	9: 3,001,350 (GRCm39)	L142S	probably benign	Het
Gm10800	T	C	2: 98,497,163 (GRCm39)	R152G	probably benign	Het
Gm10800	C	A	2: 98,497,250 (GRCm39)	V123F	probably benign	Het
Gm10800	CAAGAAAACTGAAAATCA	C	2: 98,497,361 (GRCm39)		probably null	Het
Gm10800	A	C	2: 98,496,893 (GRCm39)	F220C	probably benign	Het
Gm10801	A	G	2: 98,492,648 (GRCm39)	R23G	probably benign	Het
Gm11168	C	T	9: 3,004,605 (GRCm39)	P49S	probably benign	Het
Gm21663	C	T	5: 26,143,767 (GRCm39)	R185H	probably benign	Het
Gm21738	G	A	14: 19,417,330 (GRCm38)	S66L	probably benign	Het
Gpr107	T	A	2: 31,057,083 (GRCm39)	D58E	probably benign	Het
Gstm7	AAC	A	3: 107,838,799 (GRCm39)		probably null	Het
Hc	C	T	2: 34,921,833 (GRCm39)		probably benign	Het
Hjurp	TCTGGGAGGGCTTGCTCCGGGGGCAGTGTGTCCTGTTCTTGTGCAGCCCCTG	T	1: 88,194,000 (GRCm39)		probably benign	Het
Hjurp	A	G	1: 88,194,283 (GRCm39)		probably benign	Het
Hjurp	T	C	1: 88,194,338 (GRCm39)	E190G	probably benign	Het
Hjurp	A	C	1: 88,193,768 (GRCm39)	V380G	probably damaging	Het
Hnrnpa2b1	A	T	6: 51,441,089 (GRCm39)	M327K	probably benign	Het
Hoxa13	G	C	6: 52,260,648 (GRCm38)		probably benign	Het
Hoxa13	C	G	6: 52,260,647 (GRCm38)		probably benign	Het
Ifi206	A	G	1: 173,308,730 (GRCm39)	V422A	probably benign	Het
Igf2bp3	A	G	6: 49,094,317 (GRCm39)	V151A	probably damaging	Het
Ivl	TTGC	T	3: 92,479,608 (GRCm39)		probably benign	Het
Kndc1	TC	T	7: 139,503,692 (GRCm39)		probably null	Het
Lig1	GC	G	7: 13,039,850 (GRCm39)		probably null	Het
Lrit1	A	G	14: 36,783,998 (GRCm39)	Y442C	probably damaging	Het
Map3k21	C	A	8: 126,664,047 (GRCm39)	P536H	probably damaging	Het
Marchf10	T	A	11: 105,281,346 (GRCm39)	Y313F	probably benign	Het
Mcm5	A	G	8: 75,853,864 (GRCm39)	H706R	probably benign	Het
Mlycd	T	G	8: 120,137,199 (GRCm39)	I473S	probably damaging	Het
Muc4	T	A	16: 32,755,684 (GRCm38)		probably benign	Het
Muc4	C	A	16: 32,754,529 (GRCm38)	H1468N	probably benign	Het
Muc4	C	G	16: 32,755,676 (GRCm38)		probably benign	Het
Myo5c	T	C	9: 75,191,230 (GRCm39)	V1088A	probably benign	Het
Nadk2	TG	T	15: 9,100,232 (GRCm39)		probably null	Het
Ndufs1	A	G	1: 63,198,907 (GRCm39)		probably benign	Het
Or4n4	A	G	14: 50,518,784 (GRCm39)	*309Q	probably null	Het
Pak2	A	T	16: 31,841,930 (GRCm39)	Y443N	probably damaging	Het
Pik3r6	T	C	11: 68,417,931 (GRCm39)	I73T	probably damaging	Het
Pla2g4c	A	G	7: 13,077,316 (GRCm39)	E286G	probably benign	Het
Plekhn1	G	A	4: 156,309,397 (GRCm39)	R196*	probably null	Het
Pramel41	A	C	5: 94,594,643 (GRCm39)	K168Q	probably benign	Het
Prdm10	T	A	9: 31,237,063 (GRCm39)	D145E	probably benign	Het
Ptgis	T	C	2: 167,048,750 (GRCm39)	Y422C	probably damaging	Het
Ralgapb	A	G	2: 158,272,342 (GRCm39)	E132G	probably benign	Het
Rasgrf1	G	A	9: 89,797,626 (GRCm39)	R168H	possibly damaging	Het
Rbmyf1	T	A	Y: 2,787,132 (GRCm39)	N228Y	probably benign	Het
Rpl5	T	C	5: 108,055,049 (GRCm39)		probably benign	Het
Ryr2	T	C	13: 11,722,682 (GRCm39)	K2603E	probably damaging	Het
Sap130	T	C	18: 31,800,064 (GRCm39)		probably null	Het
Scn5a	C	A	9: 119,315,324 (GRCm39)	D1794Y	probably damaging	Het
Sec31a	A	T	5: 100,555,134 (GRCm39)	S29T	probably damaging	Het
Selplg	T	G	5: 113,957,689 (GRCm39)	K206Q	probably benign	Het
Sfmbt1	A	T	14: 30,538,714 (GRCm39)		probably null	Het
Sirpb1a	A	T	3: 15,476,258 (GRCm39)	F180I	probably benign	Het
Slc44a4	A	G	17: 35,140,251 (GRCm39)	I67V	probably damaging	Het
Sp110	T	C	1: 85,513,975 (GRCm39)	R261G	probably benign	Het
Sp110	C	T	1: 85,513,971 (GRCm39)	R262Q	probably benign	Het
Sp140	T	A	1: 85,528,893 (GRCm39)	Y5N	probably benign	Het
Sp140	A	G	1: 85,570,942 (GRCm39)	S461G	probably benign	Het
Sp140	G	C	1: 85,538,603 (GRCm39)	K113N	probably benign	Het
Sp140l1	G	A	1: 85,077,341 (GRCm39)	A75V	probably benign	Het
Sp140l2	A	C	1: 85,223,395 (GRCm39)		probably benign	Het
Speer4a2	A	T	5: 26,291,485 (GRCm39)	F107Y	probably benign	Het
Speer4a2	C	G	5: 26,294,093 (GRCm39)	W28C	probably damaging	Het
Stab2	A	T	10: 86,703,039 (GRCm39)	S1848R	possibly damaging	Het
Stat6	T	C	10: 127,494,099 (GRCm39)	V642A	possibly damaging	Het
Tfam	T	C	10: 71,070,813 (GRCm39)	K63R	possibly damaging	Het
Trim2	T	C	3: 84,098,164 (GRCm39)	N379S	probably benign	Het
Trp63	C	T	16: 25,684,013 (GRCm39)	T300I	probably damaging	Het
Tymp	C	A	15: 89,260,548 (GRCm39)	W90L	probably damaging	Het
Ubr5	A	G	15: 38,042,153 (GRCm39)	S148P		Het
Ugt1a10	TTCA	T	1: 88,143,880 (GRCm39)		probably benign	Het
Usp47	T	C	7: 111,703,548 (GRCm39)		probably benign	Het
Uvssa	C	T	5: 33,549,428 (GRCm39)	A363V	probably benign	Het
Vmn1r3	C	T	4: 3,184,691 (GRCm39)	M205I	probably damaging	Het
Vmn1r3	C	T	4: 3,184,774 (GRCm39)	V178I	probably benign	Het
Vmn1r67	T	A	7: 10,180,877 (GRCm39)	M47K	probably benign	Het
Vmn1r87	A	T	7: 12,866,112 (GRCm39)	H58Q	probably benign	Het
Vmn1r89	A	G	7: 12,953,515 (GRCm39)	T84A	probably benign	Het
Vmn2r109	T	C	17: 20,774,839 (GRCm39)		probably null	Het
Xirp2	T	C	2: 67,349,706 (GRCm39)		probably benign	Het
Zbtb2	A	G	10: 4,319,493 (GRCm39)	S178P	probably benign	Het
Zfp534	C	A	4: 147,762,770 (GRCm39)	D21Y	probably benign	Het
Zfp534	C	T	4: 147,760,031 (GRCm39)	E213K	probably benign	Het
Zfp804b	C	T	5: 6,819,422 (GRCm39)	V1214I	probably damaging	Het
Zfp82	G	T	7: 29,756,701 (GRCm39)	T63K	probably damaging	Het
Zfp986	C	T	4: 145,625,513 (GRCm39)	R58C	probably benign	Het
Zfp992	C	T	4: 146,550,569 (GRCm39)	P97S	probably benign	Het

Other mutations in Vldlr

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01346:Vldlr	APN	19	27,217,081 (GRCm39)	missense	possibly damaging	0.93
IGL01575:Vldlr	APN	19	27,224,031 (GRCm39)	missense	probably benign
IGL01626:Vldlr	APN	19	27,221,173 (GRCm39)	missense	probably damaging	1.00
IGL02213:Vldlr	APN	19	27,218,726 (GRCm39)	missense	probably benign	0.09
IGL02365:Vldlr	APN	19	27,223,025 (GRCm39)	missense	probably damaging	1.00
IGL02488:Vldlr	APN	19	27,215,675 (GRCm39)	missense	probably damaging	1.00
IGL02708:Vldlr	APN	19	27,215,485 (GRCm39)	missense	possibly damaging	0.92
IGL02947:Vldlr	APN	19	27,217,120 (GRCm39)	missense	probably benign	0.03
disturbed	UTSW	19	27,216,204 (GRCm39)	nonsense	probably null
r26	UTSW	19	27,223,054 (GRCm39)	missense	probably damaging	0.99
spotty	UTSW	19	27,216,192 (GRCm39)	missense	probably damaging	1.00
R0195:Vldlr	UTSW	19	27,215,786 (GRCm39)	missense	probably damaging	1.00
R0288:Vldlr	UTSW	19	27,218,051 (GRCm39)	splice site	probably benign
R0536:Vldlr	UTSW	19	27,217,364 (GRCm39)	missense	probably damaging	1.00
R0537:Vldlr	UTSW	19	27,225,318 (GRCm39)	missense	probably damaging	1.00
R0542:Vldlr	UTSW	19	27,213,655 (GRCm39)	missense	probably benign	0.01
R0594:Vldlr	UTSW	19	27,212,219 (GRCm39)	missense	probably damaging	1.00
R0624:Vldlr	UTSW	19	27,215,663 (GRCm39)	missense	possibly damaging	0.91
R0726:Vldlr	UTSW	19	27,215,786 (GRCm39)	missense	probably damaging	1.00
R1017:Vldlr	UTSW	19	27,218,733 (GRCm39)	missense	probably damaging	1.00
R1148:Vldlr	UTSW	19	27,218,691 (GRCm39)	missense	probably benign	0.01
R1148:Vldlr	UTSW	19	27,218,691 (GRCm39)	missense	probably benign	0.01
R1443:Vldlr	UTSW	19	27,217,121 (GRCm39)	missense	possibly damaging	0.91
R1493:Vldlr	UTSW	19	27,218,691 (GRCm39)	missense	probably benign	0.01
R1520:Vldlr	UTSW	19	27,224,466 (GRCm39)	missense	possibly damaging	0.96
R1520:Vldlr	UTSW	19	27,217,943 (GRCm39)	missense	probably damaging	0.99
R1657:Vldlr	UTSW	19	27,223,070 (GRCm39)	missense	probably benign	0.00
R1901:Vldlr	UTSW	19	27,218,709 (GRCm39)	missense	probably damaging	1.00
R2047:Vldlr	UTSW	19	27,212,238 (GRCm39)	missense	probably damaging	1.00
R2258:Vldlr	UTSW	19	27,215,786 (GRCm39)	missense	probably damaging	1.00
R2273:Vldlr	UTSW	19	27,225,415 (GRCm39)	missense	probably damaging	1.00
R2423:Vldlr	UTSW	19	27,213,688 (GRCm39)	missense	possibly damaging	0.49
R3196:Vldlr	UTSW	19	27,220,554 (GRCm39)	missense	probably damaging	0.98
R3752:Vldlr	UTSW	19	27,215,731 (GRCm39)	missense	probably damaging	1.00
R3801:Vldlr	UTSW	19	27,195,021 (GRCm39)	missense	probably damaging	0.99
R3835:Vldlr	UTSW	19	27,212,214 (GRCm39)	missense	probably damaging	1.00
R4027:Vldlr	UTSW	19	27,215,713 (GRCm39)	missense	probably benign
R4301:Vldlr	UTSW	19	27,215,802 (GRCm39)	missense	possibly damaging	0.80
R4470:Vldlr	UTSW	19	27,212,219 (GRCm39)	missense	probably damaging	0.96
R4541:Vldlr	UTSW	19	27,216,192 (GRCm39)	missense	probably damaging	1.00
R4765:Vldlr	UTSW	19	27,217,947 (GRCm39)	missense	probably damaging	1.00
R4771:Vldlr	UTSW	19	27,217,290 (GRCm39)	missense	probably damaging	0.97
R4795:Vldlr	UTSW	19	27,216,252 (GRCm39)	splice site	probably null
R4839:Vldlr	UTSW	19	27,215,465 (GRCm39)	missense	probably damaging	1.00
R5074:Vldlr	UTSW	19	27,215,677 (GRCm39)	missense	probably damaging	1.00
R5134:Vldlr	UTSW	19	27,216,212 (GRCm39)	nonsense	probably null
R5281:Vldlr	UTSW	19	27,221,631 (GRCm39)	missense	probably benign	0.44
R5466:Vldlr	UTSW	19	27,217,243 (GRCm39)	critical splice acceptor site	probably null
R5514:Vldlr	UTSW	19	27,221,624 (GRCm39)	missense	probably damaging	0.97
R5886:Vldlr	UTSW	19	27,221,171 (GRCm39)	missense	probably benign	0.03
R5889:Vldlr	UTSW	19	27,217,064 (GRCm39)	missense	probably damaging	1.00
R6110:Vldlr	UTSW	19	27,215,477 (GRCm39)	missense	possibly damaging	0.92
R6343:Vldlr	UTSW	19	27,223,049 (GRCm39)	missense	probably damaging	0.99
R6833:Vldlr	UTSW	19	27,217,974 (GRCm39)	missense	probably damaging	1.00
R6838:Vldlr	UTSW	19	27,225,370 (GRCm39)	missense	probably damaging	1.00
R7169:Vldlr	UTSW	19	27,221,728 (GRCm39)	missense	probably benign
R7197:Vldlr	UTSW	19	27,212,241 (GRCm39)	missense	probably benign	0.36
R7304:Vldlr	UTSW	19	27,216,004 (GRCm39)	missense	possibly damaging	0.93
R7403:Vldlr	UTSW	19	27,213,674 (GRCm39)	nonsense	probably null
R7658:Vldlr	UTSW	19	27,220,536 (GRCm39)	missense	probably benign	0.33
R7754:Vldlr	UTSW	19	27,195,015 (GRCm39)	start codon destroyed	probably benign	0.01
R8105:Vldlr	UTSW	19	27,216,204 (GRCm39)	nonsense	probably null
R8377:Vldlr	UTSW	19	27,212,258 (GRCm39)	missense	probably damaging	1.00
R8529:Vldlr	UTSW	19	27,207,656 (GRCm39)	missense	probably benign	0.03
R8777:Vldlr	UTSW	19	27,217,946 (GRCm39)	missense	probably benign	0.00
R8777-TAIL:Vldlr	UTSW	19	27,217,946 (GRCm39)	missense	probably benign	0.00
R9380:Vldlr	UTSW	19	27,216,192 (GRCm39)	missense	possibly damaging	0.63
R9400:Vldlr	UTSW	19	27,216,175 (GRCm39)	missense	probably damaging	0.99
R9483:Vldlr	UTSW	19	27,224,031 (GRCm39)	missense	probably benign	0.00
R9502:Vldlr	UTSW	19	27,218,742 (GRCm39)	missense	probably damaging	1.00
R9509:Vldlr	UTSW	19	27,221,687 (GRCm39)	missense	probably benign	0.44
R9630:Vldlr	UTSW	19	27,207,623 (GRCm39)	missense	probably damaging	1.00
R9767:Vldlr	UTSW	19	27,212,274 (GRCm39)	missense	probably damaging	1.00
R9768:Vldlr	UTSW	19	27,218,720 (GRCm39)	missense	possibly damaging	0.47

Predicted Primers

PCR Primer
(F):5'- CCCTAGAGATGCCAACAATTTG -3'
(R):5'- GTCAGTTGGCCTCAAGTTTCAG -3'

Sequencing Primer
(F):5'- GCCAACAATTTGAATATCAGTCAGC -3'
(R):5'- CCTCAAGTTTCAGGCAGGCAAAG -3'

Posted On

2017-09-21