Mutagenetix > Incidental Mutation

Incidental Mutation 'R6142:Zfp773'

488625

Institutional Source

Beutler Lab

Gene Symbol

Zfp773

Ensembl Gene

ENSMUSG00000063535

Gene Name

zinc finger protein 773

Synonyms

2810409K11Rik

MMRRC Submission

044289-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R6142 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

7133677-7139754 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 7135481 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 372 (T372A)

Ref Sequence

ENSEMBL: ENSMUSP00000032622 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032622] [ENSMUST00000211240]

AlphaFold

Q9CZ29

Predicted Effect

probably benign
Transcript: ENSMUST00000032622
AA Change: T372A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000032622
Gene: ENSMUSG00000063535
AA Change: T372A

Domain	Start	End	E-Value	Type
KRAB	75	134	6.82e-8	SMART
ZnF_C2H2	241	263	1.31e0	SMART
ZnF_C2H2	269	291	1.5e-4	SMART
ZnF_C2H2	297	319	4.17e-3	SMART
ZnF_C2H2	325	347	2.05e-2	SMART
ZnF_C2H2	353	375	2.24e-3	SMART
ZnF_C2H2	381	403	8.81e-2	SMART
ZnF_C2H2	409	431	7.26e-3	SMART
ZnF_C2H2	437	459	7.26e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000211240

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.9%
20x: 94.2%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy6	T	C	15: 98,496,303 (GRCm39)	M579V	probably benign	Het
Agtr1b	T	C	3: 20,370,558 (GRCm39)	D16G	probably benign	Het
Akap12	C	A	10: 4,263,740 (GRCm39)		probably null	Het
Alpk2	T	C	18: 65,438,456 (GRCm39)	E979G	possibly damaging	Het
Arfgap3	T	C	15: 83,234,328 (GRCm39)	D8G	probably damaging	Het
AW551984	G	T	9: 39,508,410 (GRCm39)	L369I	probably benign	Het
Bmp8b	A	T	4: 123,009,043 (GRCm39)	H207L	probably benign	Het
Cc2d2a	G	T	5: 43,860,540 (GRCm39)	V490F	probably damaging	Het
Cnot6l	C	T	5: 96,230,837 (GRCm39)	V377I	probably benign	Het
Col25a1	T	A	3: 130,376,978 (GRCm39)		probably benign	Het
Crocc2	C	G	1: 93,118,201 (GRCm39)	Q375E	possibly damaging	Het
Ganc	T	C	2: 120,261,218 (GRCm39)		probably null	Het
Gm28168	C	A	1: 117,875,678 (GRCm39)	D102E	probably benign	Het
Gm8212	T	C	14: 44,438,684 (GRCm39)		probably null	Het
Gria2	T	C	3: 80,709,024 (GRCm39)	T53A	probably benign	Het
Hipk4	G	A	7: 27,228,590 (GRCm39)	V347M	probably damaging	Het
Hmgxb3	T	C	18: 61,269,309 (GRCm39)	E991G	probably benign	Het
Hyal1	G	A	9: 107,456,573 (GRCm39)	R420H	probably benign	Het
Il5	G	A	11: 53,611,805 (GRCm39)		probably null	Het
Kcnh7	T	C	2: 62,569,704 (GRCm39)	I731V	possibly damaging	Het
Lamb2	A	T	9: 108,362,817 (GRCm39)	K759*	probably null	Het
Lnpep	A	G	17: 17,786,943 (GRCm39)		probably null	Het
Lrch1	T	C	14: 75,184,940 (GRCm39)	E54G	probably damaging	Het
Msl3l2	A	G	10: 55,991,461 (GRCm39)	D62G	possibly damaging	Het
Mslnl	T	C	17: 25,963,531 (GRCm39)	L339P	probably damaging	Het
Nfe2l2	T	C	2: 75,509,761 (GRCm39)	I20V	probably damaging	Het
Pgm5	A	C	19: 24,801,772 (GRCm39)	I152S	probably damaging	Het
Plcg2	C	A	8: 118,312,010 (GRCm39)	T434K	probably benign	Het
Prrc2c	T	C	1: 162,537,956 (GRCm39)	E558G	unknown	Het
Ranbp3	T	C	17: 56,993,018 (GRCm39)	V12A	probably benign	Het
Rgsl1	A	G	1: 153,687,984 (GRCm39)	V635A	probably benign	Het
Rpap2	T	C	5: 107,746,164 (GRCm39)	V14A	probably benign	Het
Rxrg	G	T	1: 167,460,191 (GRCm39)	A341S	possibly damaging	Het
Sbf2	A	G	7: 109,948,182 (GRCm39)	S1177P	probably damaging	Het
Sema6a	T	C	18: 47,414,266 (GRCm39)	T498A	probably benign	Het
Slc13a4	G	A	6: 35,278,718 (GRCm39)	A57V	probably damaging	Het
Slc6a3	A	T	13: 73,692,902 (GRCm39)	D174V	probably benign	Het
Spart	T	G	3: 55,024,669 (GRCm39)	V88G	probably damaging	Het
Tanc1	A	T	2: 59,663,566 (GRCm39)	R1108*	probably null	Het
Tas2r140	C	T	6: 133,032,698 (GRCm39)	G20E	probably damaging	Het
Tdrd6	A	G	17: 43,940,373 (GRCm39)	V225A	probably benign	Het
Tmem168	C	A	6: 13,591,368 (GRCm39)	A99S	probably benign	Het
Tnk2	T	A	16: 32,488,917 (GRCm39)	D252E	probably damaging	Het
Tpsg1	A	T	17: 25,591,460 (GRCm39)	H20L	probably benign	Het
Tram1l1	T	C	3: 124,115,092 (GRCm39)	F84S	probably damaging	Het
Vmn2r12	T	A	5: 109,240,763 (GRCm39)	I117L	probably benign	Het
Zfp148	T	C	16: 33,315,829 (GRCm39)	L219S	possibly damaging	Het
Zfp40	C	A	17: 23,395,311 (GRCm39)	E425D	probably benign	Het

Other mutations in Zfp773

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00764:Zfp773	APN	7	7,135,683 (GRCm39)	missense	probably damaging	1.00
IGL00780:Zfp773	APN	7	7,136,113 (GRCm39)	missense	probably benign	0.00
IGL01348:Zfp773	APN	7	7,138,314 (GRCm39)	missense	possibly damaging	0.93
IGL02224:Zfp773	APN	7	7,135,975 (GRCm39)	missense	probably benign	0.00
IGL02447:Zfp773	APN	7	7,139,655 (GRCm39)	utr 5 prime	probably benign
IGL02869:Zfp773	APN	7	7,137,232 (GRCm39)	missense	probably benign	0.22
R0505:Zfp773	UTSW	7	7,136,023 (GRCm39)	missense	probably benign	0.03
R0585:Zfp773	UTSW	7	7,135,574 (GRCm39)	missense	probably benign	0.21
R0804:Zfp773	UTSW	7	7,136,092 (GRCm39)	intron	probably benign
R0846:Zfp773	UTSW	7	7,135,691 (GRCm39)	missense	probably damaging	1.00
R1179:Zfp773	UTSW	7	7,136,092 (GRCm39)	intron	probably benign
R2847:Zfp773	UTSW	7	7,136,092 (GRCm39)	intron	probably benign
R3841:Zfp773	UTSW	7	7,135,390 (GRCm39)	missense	possibly damaging	0.92
R4116:Zfp773	UTSW	7	7,136,092 (GRCm39)	intron	probably benign
R4638:Zfp773	UTSW	7	7,138,335 (GRCm39)	missense	probably damaging	1.00
R5126:Zfp773	UTSW	7	7,139,623 (GRCm39)	missense	unknown
R7072:Zfp773	UTSW	7	7,135,874 (GRCm39)	missense	probably benign	0.15
R7232:Zfp773	UTSW	7	7,135,984 (GRCm39)	missense	probably benign	0.14
R7748:Zfp773	UTSW	7	7,135,907 (GRCm39)	missense	probably benign	0.04
R7888:Zfp773	UTSW	7	7,135,978 (GRCm39)	missense	probably benign	0.00
R8681:Zfp773	UTSW	7	7,139,482 (GRCm39)	missense	possibly damaging	0.70
R8784:Zfp773	UTSW	7	7,135,570 (GRCm39)	missense	probably benign	0.19
R8946:Zfp773	UTSW	7	7,135,469 (GRCm39)	missense	possibly damaging	0.82
R9056:Zfp773	UTSW	7	7,135,989 (GRCm39)	missense	probably damaging	0.99
R9154:Zfp773	UTSW	7	7,138,302 (GRCm39)	missense	probably damaging	0.98
R9295:Zfp773	UTSW	7	7,135,694 (GRCm39)	missense	probably benign	0.06
RF007:Zfp773	UTSW	7	7,135,689 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GTCCAGTCCTGTAGCAAAACAC -3'
(R):5'- TGTGAGAAGTCCTTCATCCAAAAG -3'

Sequencing Primer
(F):5'- CTGCAGTGTGAAATCTCTGATGC -3'
(R):5'- CCTTGTTGGGCATCAAAGAC -3'

Posted On

2017-10-10