|Institutional Source||Beutler Lab|
|Gene Name||interleukin 5|
|Is this an essential gene?||Probably non essential (E-score: 0.145)|
|Stock #||R6142 (G1)|
|Chromosomal Location||53720794-53725106 bp(+) (GRCm38)|
|Type of Mutation||critical splice donor site (1 bp from exon)|
|DNA Base Change (assembly)||G to A at 53720978 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000043369 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000048605]|
|Predicted Effect||probably null
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.514|
|Coding Region Coverage||
|Validation Efficiency||100% (48/48)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit loss of normal airway hyperreactivity resulting from aeroallergen challenge, reduced numbers of CD5+ B cells in the peritoneal cavity at 2 weeks, and some altered responses to schistosomiasis infection. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Il5||
(F):5'- AAATTGCTTGGGGATTCGGC -3'
(R):5'- GCAGCTATTTATGCATTCTGAAGAACC -3'
(F):5'- GATTCGGCCCTGCTCTG -3'
(R):5'- TGCATTCTGAAGAACCATAGCGTC -3'