Mutagenetix > Incidental Mutation

Incidental Mutation 'R6148:Vmn2r55'

489018

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r55

Ensembl Gene

ENSMUSG00000091045

Gene Name

vomeronasal 2, receptor 55

Synonyms

MMRRC Submission

044295-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.098) question?

Stock #

R6148 (G1)

Quality Score

114.008

Status

Not validated

Chromosome

Chromosomal Location

12385633-12422855 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 12402069 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 406 (L406F)

Ref Sequence

ENSEMBL: ENSMUSP00000133483 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167771] [ENSMUST00000172743]

AlphaFold

A0A3B2W3J6

Predicted Effect

probably benign
Transcript: ENSMUST00000167771
AA Change: L406F

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000132834
Gene: ENSMUSG00000091045
AA Change: L406F

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	5	398	6.1e-54	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000172743
AA Change: L406F

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000133483
Gene: ENSMUSG00000091045
AA Change: L406F

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	5	397	3.4e-57	PFAM
Pfam:7tm_3	525	762	3.7e-54	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.6%

Validation Efficiency

95% (61/64)

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	T	A	12: 71,234,200 (GRCm39)	C1067S	possibly damaging	Het
AAdacl4fm3	T	C	4: 144,447,887 (GRCm39)	T30A	possibly damaging	Het
Agbl3	T	A	6: 34,834,688 (GRCm39)	S952R	possibly damaging	Het
Ano9	A	G	7: 140,686,698 (GRCm39)	Y429H	probably damaging	Het
Bbs7	C	A	3: 36,667,415 (GRCm39)	R7L	probably damaging	Het
Birc2	T	A	9: 7,849,684 (GRCm39)	D535V	possibly damaging	Het
Catsper4	A	G	4: 133,945,240 (GRCm39)	V206A	probably damaging	Het
Ccdc73	T	G	2: 104,822,482 (GRCm39)	S810R	possibly damaging	Het
Cd177	A	T	7: 24,443,698 (GRCm39)	L800*	probably null	Het
Cd34	T	C	1: 194,630,316 (GRCm39)		probably null	Het
Cep78	G	A	19: 15,959,150 (GRCm39)	R95*	probably null	Het
Cfap69	T	C	5: 5,713,996 (GRCm39)	D12G	probably benign	Het
Cpne7	A	G	8: 123,854,171 (GRCm39)	D286G	probably benign	Het
Cwc22	ATCTCTCTCTCTCTCTCT	ATCTCTCTCTCTCTCT	2: 77,759,803 (GRCm39)		probably null	Het
Cxcl5	A	T	5: 90,907,565 (GRCm39)	I46L	probably benign	Het
Cyp19a1	C	T	9: 54,087,540 (GRCm39)	G59D	probably damaging	Het
Dclre1c	A	G	2: 3,438,742 (GRCm39)	D35G	probably damaging	Het
Defa41	A	G	8: 21,692,428 (GRCm39)	N83S	probably benign	Het
Dvl1	A	G	4: 155,939,409 (GRCm39)	Y279C	probably damaging	Het
Fes	A	G	7: 80,030,044 (GRCm39)	L578P	probably damaging	Het
Fkbpl	G	A	17: 34,864,303 (GRCm39)	A24T	probably benign	Het
Fmn2	T	C	1: 174,494,229 (GRCm39)	S1248P	probably damaging	Het
Fmo1	T	G	1: 162,679,088 (GRCm39)	S53R	probably damaging	Het
Gabrg1	A	T	5: 70,931,804 (GRCm39)	M313K	probably damaging	Het
Galnt10	T	C	11: 57,675,474 (GRCm39)	C578R	probably damaging	Het
Gpr39	T	C	1: 125,800,323 (GRCm39)	V358A	probably damaging	Het
Gsap	A	G	5: 21,431,323 (GRCm39)	R216G	probably damaging	Het
Gsap	A	T	5: 21,475,575 (GRCm39)	T570S	probably benign	Het
Gucy2d	A	T	7: 98,093,030 (GRCm39)	I136L	probably benign	Het
Hap1	A	T	11: 100,240,218 (GRCm39)	V294E	probably damaging	Het
Ipo8	A	G	6: 148,701,278 (GRCm39)	V514A	probably damaging	Het
Irf5	T	C	6: 29,535,958 (GRCm39)	L324P	probably damaging	Het
Itsn1	C	T	16: 91,613,740 (GRCm39)	R251C	probably damaging	Het
Klf11	T	C	12: 24,701,567 (GRCm39)		probably null	Het
Kpna6	G	A	4: 129,543,099 (GRCm39)	Q439*	probably null	Het
Mark4	A	T	7: 19,163,441 (GRCm39)	S563T	probably benign	Het
Mrpl21	A	T	19: 3,333,084 (GRCm39)	I5L	probably benign	Het
Muc4	T	C	16: 32,753,802 (GRCm38)	I1226T	probably benign	Het
Myrf	G	A	19: 10,189,839 (GRCm39)	T815I	probably damaging	Het
Olfr515-ps1	A	T	7: 108,444,178 (GRCm39)		probably null	Het
Or10d4c	G	T	9: 39,558,555 (GRCm39)	D178Y	probably damaging	Het
Or11g27	A	G	14: 50,771,778 (GRCm39)	N303S	probably benign	Het
Or4c15	C	T	2: 88,760,597 (GRCm39)	V21I	probably benign	Het
Or52n3	A	G	7: 104,530,289 (GRCm39)	Y125C	possibly damaging	Het
Os9	T	C	10: 126,935,812 (GRCm39)	D280G	probably benign	Het
Pcdhb14	A	G	18: 37,582,283 (GRCm39)	N463S	probably damaging	Het
Pex19	G	A	1: 171,961,606 (GRCm39)	E271K	probably damaging	Het
Ppdpf	G	A	2: 180,829,641 (GRCm39)	S32N	probably benign	Het
Prdm2	A	G	4: 142,859,477 (GRCm39)	I1271T	probably benign	Het
Rbbp4	T	C	4: 129,215,751 (GRCm39)	T262A	probably benign	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Spaca9	G	A	2: 28,583,793 (GRCm39)	R64W	probably damaging	Het
Sult1e1	A	T	5: 87,727,770 (GRCm39)	S171T	probably damaging	Het
Tns1	T	C	1: 73,992,612 (GRCm39)	T689A	probably damaging	Het
Unc13c	G	C	9: 73,600,648 (GRCm39)	N1365K	probably benign	Het
Vmn2r52	T	C	7: 9,905,090 (GRCm39)	I250V	probably benign	Het
Wdcp	T	C	12: 4,900,621 (GRCm39)	V159A	possibly damaging	Het
Zbtb3	C	T	19: 8,781,560 (GRCm39)	A391V	probably benign	Het
Znhit1	A	T	5: 137,011,487 (GRCm39)	S109T	probably benign	Het

Other mutations in Vmn2r55

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02684:Vmn2r55	APN	7	12,404,887 (GRCm39)	missense	probably damaging	1.00
IGL03035:Vmn2r55	APN	7	12,404,743 (GRCm39)	missense	probably benign	0.01
IGL03115:Vmn2r55	APN	7	12,404,558 (GRCm39)	missense	probably damaging	1.00
IGL03251:Vmn2r55	APN	7	12,405,120 (GRCm39)	splice site	probably benign
R0140:Vmn2r55	UTSW	7	12,402,104 (GRCm39)	missense	possibly damaging	0.58
R0511:Vmn2r55	UTSW	7	12,404,945 (GRCm39)	missense	possibly damaging	0.88
R1281:Vmn2r55	UTSW	7	12,404,825 (GRCm39)	missense	probably benign	0.02
R1564:Vmn2r55	UTSW	7	12,418,678 (GRCm39)	missense	probably damaging	1.00
R1602:Vmn2r55	UTSW	7	12,386,571 (GRCm39)	missense	probably damaging	1.00
R1785:Vmn2r55	UTSW	7	12,402,111 (GRCm39)	missense	probably damaging	0.98
R2939:Vmn2r55	UTSW	7	12,385,832 (GRCm39)	missense	probably damaging	1.00
R2993:Vmn2r55	UTSW	7	12,418,882 (GRCm39)	missense	probably damaging	1.00
R3151:Vmn2r55	UTSW	7	12,404,634 (GRCm39)	missense	probably benign	0.06
R4272:Vmn2r55	UTSW	7	12,402,106 (GRCm39)	missense	probably benign	0.38
R4589:Vmn2r55	UTSW	7	12,404,822 (GRCm39)	missense	probably damaging	0.99
R4624:Vmn2r55	UTSW	7	12,404,627 (GRCm39)	missense	possibly damaging	0.83
R4965:Vmn2r55	UTSW	7	12,404,478 (GRCm39)	missense	possibly damaging	0.91
R5294:Vmn2r55	UTSW	7	12,385,791 (GRCm39)	missense	probably damaging	1.00
R5364:Vmn2r55	UTSW	7	12,404,830 (GRCm39)	missense	possibly damaging	0.51
R5395:Vmn2r55	UTSW	7	12,385,874 (GRCm39)	missense	probably damaging	1.00
R5401:Vmn2r55	UTSW	7	12,385,871 (GRCm39)	missense	probably benign
R5701:Vmn2r55	UTSW	7	12,404,492 (GRCm39)	missense	probably benign	0.00
R5771:Vmn2r55	UTSW	7	12,404,959 (GRCm39)	missense	probably damaging	1.00
R5846:Vmn2r55	UTSW	7	12,404,492 (GRCm39)	missense	probably benign	0.05
R6159:Vmn2r55	UTSW	7	12,385,698 (GRCm39)	missense	probably damaging	1.00
R6541:Vmn2r55	UTSW	7	12,404,939 (GRCm39)	missense	probably damaging	1.00
R7286:Vmn2r55	UTSW	7	12,386,000 (GRCm39)	missense	probably damaging	0.99
R7483:Vmn2r55	UTSW	7	12,404,755 (GRCm39)	missense	probably benign	0.00
R8269:Vmn2r55	UTSW	7	12,404,585 (GRCm39)	missense	possibly damaging	0.86
R8749:Vmn2r55	UTSW	7	12,385,796 (GRCm39)	missense	probably damaging	1.00
R8914:Vmn2r55	UTSW	7	12,405,024 (GRCm39)	missense	probably benign	0.09
R9049:Vmn2r55	UTSW	7	12,418,908 (GRCm39)	missense	probably damaging	1.00
R9175:Vmn2r55	UTSW	7	12,385,793 (GRCm39)	missense	possibly damaging	0.67
R9344:Vmn2r55	UTSW	7	12,385,782 (GRCm39)	nonsense	probably null
R9498:Vmn2r55	UTSW	7	12,404,812 (GRCm39)	missense	probably damaging	1.00
R9715:Vmn2r55	UTSW	7	12,402,061 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r55	UTSW	7	12,405,106 (GRCm39)	missense	possibly damaging	0.91
Z1177:Vmn2r55	UTSW	7	12,385,764 (GRCm39)	missense	possibly damaging	0.83

Predicted Primers

PCR Primer
(F):5'- TGAATGGCCTACATCCACTTC -3'
(R):5'- ATCCCTTCAGACACCATTTGGAC -3'

Sequencing Primer
(F):5'- TCCACTTCATGCATAAGTAGACAGG -3'
(R):5'- TTTGGACAAGACACTGAACAGTC -3'

Posted On

2017-10-10