Mutagenetix > Incidental Mutation

Incidental Mutation 'R6162:Tas2r134'

489906

Institutional Source

Beutler Lab

Gene Symbol

Tas2r134

Ensembl Gene

ENSMUSG00000056115

Gene Name

taste receptor, type 2, member 134

Synonyms

Tas2r34, T2R134

MMRRC Submission

044309-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R6162 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

51517523-51518419 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 51517571 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 17 (S17P)

Ref Sequence

ENSEMBL: ENSMUSP00000064657 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000070028]

AlphaFold

Q7TQB0

Predicted Effect

probably damaging
Transcript: ENSMUST00000070028
AA Change: S17P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000064657
Gene: ENSMUSG00000056115
AA Change: S17P

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	291	1.4e-75	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 94.1%

Validation Efficiency

98% (40/41)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	G	11: 9,259,047 (GRCm39)	C2924W	probably damaging	Het
Adh4	T	A	3: 138,121,250 (GRCm39)		probably null	Het
Aox3	A	T	1: 58,198,890 (GRCm39)	D695V	possibly damaging	Het
Ccnj	A	G	19: 40,833,606 (GRCm39)	Y221C	probably damaging	Het
Cct2	T	A	10: 116,894,091 (GRCm39)	D221V	probably damaging	Het
Cd5	G	A	19: 10,703,244 (GRCm39)	T138M	probably damaging	Het
Cep78	A	T	19: 15,952,304 (GRCm39)	M307K	probably benign	Het
Dclk1	C	A	3: 55,163,575 (GRCm39)	D222E	probably benign	Het
Dhrs11	C	T	11: 84,719,605 (GRCm39)	G40D	probably damaging	Het
Dnah10	A	G	5: 124,900,382 (GRCm39)	S3823G	probably benign	Het
Dnai3	T	A	3: 145,750,617 (GRCm39)	I821F	probably damaging	Het
Dock3	A	T	9: 106,841,998 (GRCm39)	Y944N	possibly damaging	Het
Dst	A	G	1: 34,045,318 (GRCm39)	D17G	probably damaging	Het
Ehmt2	C	T	17: 35,118,067 (GRCm39)	R40*	probably null	Het
Fam20a	G	T	11: 109,573,696 (GRCm39)	S229*	probably null	Het
Fbn1	T	C	2: 125,202,147 (GRCm39)	D1242G	probably damaging	Het
Ggt5	T	A	10: 75,425,626 (GRCm39)	V28D	possibly damaging	Het
Gimap4	A	T	6: 48,667,655 (GRCm39)	I137F	probably damaging	Het
Git2	T	A	5: 114,899,717 (GRCm39)	H212L	probably damaging	Het
Gprc6a	T	A	10: 51,491,008 (GRCm39)	I739F	probably benign	Het
Il1rl2	A	G	1: 40,391,038 (GRCm39)	Y327C	probably damaging	Het
Lmf1	A	G	17: 25,831,368 (GRCm39)	I205V	probably benign	Het
Mipep	C	A	14: 61,024,853 (GRCm39)	P98Q	probably damaging	Het
Mroh2b	A	G	15: 4,944,707 (GRCm39)	D436G	probably damaging	Het
Mtcl2	T	A	2: 156,881,784 (GRCm39)	E756V	possibly damaging	Het
Myorg	A	G	4: 41,497,899 (GRCm39)	V577A	possibly damaging	Het
Notch1	C	T	2: 26,352,207 (GRCm39)	V2035I	probably benign	Het
Or1e28-ps1	T	A	11: 73,615,713 (GRCm39)	I46F	probably damaging	Het
Or2ag16	A	T	7: 106,352,227 (GRCm39)	Y123N	probably damaging	Het
Or4c118	G	T	2: 88,975,114 (GRCm39)	F84L	probably benign	Het
Oxa1l	A	G	14: 54,605,789 (GRCm39)	T395A	probably damaging	Het
Phospho2	T	A	2: 69,626,375 (GRCm39)	I177K	probably damaging	Het
Prpf40a	T	C	2: 53,049,317 (GRCm39)	T224A	probably benign	Het
Reln	T	A	5: 22,116,048 (GRCm39)	T2987S	probably damaging	Het
Scn5a	T	A	9: 119,351,621 (GRCm39)	I787F	probably damaging	Het
Sgsm2	A	T	11: 74,782,847 (GRCm39)	D36E	probably damaging	Het
Slc12a3	A	G	8: 95,072,401 (GRCm39)		probably null	Het
Top3a	T	C	11: 60,636,763 (GRCm39)	Y609C	probably damaging	Het
Trem2	A	G	17: 48,655,694 (GRCm39)	I84V	probably damaging	Het
Vmn2r12	A	G	5: 109,234,430 (GRCm39)	L594P	probably damaging	Het
Zdbf2	A	T	1: 63,319,977 (GRCm39)		probably benign	Het
Zfr	C	T	15: 12,146,331 (GRCm39)	A294V	unknown	Het

Other mutations in Tas2r134

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00562:Tas2r134	APN	2	51,518,100 (GRCm39)	missense	possibly damaging	0.90
IGL00563:Tas2r134	APN	2	51,518,100 (GRCm39)	missense	possibly damaging	0.90
IGL01121:Tas2r134	APN	2	51,518,001 (GRCm39)	missense	probably damaging	1.00
IGL01122:Tas2r134	APN	2	51,517,671 (GRCm39)	missense	probably damaging	1.00
IGL01128:Tas2r134	APN	2	51,517,671 (GRCm39)	missense	probably damaging	1.00
IGL01132:Tas2r134	APN	2	51,517,671 (GRCm39)	missense	probably damaging	1.00
IGL01312:Tas2r134	APN	2	51,518,247 (GRCm39)	missense	probably damaging	1.00
IGL01419:Tas2r134	APN	2	51,517,759 (GRCm39)	nonsense	probably null
IGL01774:Tas2r134	APN	2	51,518,160 (GRCm39)	missense	probably benign	0.07
IGL02735:Tas2r134	APN	2	51,517,839 (GRCm39)	missense	probably damaging	1.00
R0799:Tas2r134	UTSW	2	51,518,385 (GRCm39)	missense	probably benign	0.00
R1205:Tas2r134	UTSW	2	51,517,998 (GRCm39)	missense	probably benign	0.00
R2267:Tas2r134	UTSW	2	51,518,249 (GRCm39)	missense	probably benign	0.01
R3736:Tas2r134	UTSW	2	51,517,786 (GRCm39)	missense	probably damaging	1.00
R5254:Tas2r134	UTSW	2	51,517,559 (GRCm39)	missense	probably benign	0.39
R5752:Tas2r134	UTSW	2	51,517,880 (GRCm39)	missense	probably damaging	1.00
R6940:Tas2r134	UTSW	2	51,518,148 (GRCm39)	missense	probably benign	0.00
R6954:Tas2r134	UTSW	2	51,517,782 (GRCm39)	missense	probably benign	0.00
R6996:Tas2r134	UTSW	2	51,517,601 (GRCm39)	missense	probably benign	0.00
R7206:Tas2r134	UTSW	2	51,518,120 (GRCm39)	missense	probably benign	0.02
R7303:Tas2r134	UTSW	2	51,518,145 (GRCm39)	missense	probably benign	0.01
R7348:Tas2r134	UTSW	2	51,518,414 (GRCm39)	missense	possibly damaging	0.92
R7479:Tas2r134	UTSW	2	51,517,541 (GRCm39)	missense	not run
R7575:Tas2r134	UTSW	2	51,518,166 (GRCm39)	missense	probably damaging	1.00
R7686:Tas2r134	UTSW	2	51,518,255 (GRCm39)	missense	possibly damaging	0.54
R8721:Tas2r134	UTSW	2	51,517,571 (GRCm39)	missense	probably damaging	1.00
R9384:Tas2r134	UTSW	2	51,518,034 (GRCm39)	missense	probably damaging	0.97
R9622:Tas2r134	UTSW	2	51,518,358 (GRCm39)	missense	possibly damaging	0.89

Predicted Primers

PCR Primer
(F):5'- GGATTCAGCAAGGTGATTGGC -3'
(R):5'- AACCAGAAATTGACAGTGTTGG -3'

Sequencing Primer
(F):5'- GGCTAAATAAATATGCTGTGCACCC -3'
(R):5'- AAATTGACAGTGTTGGTGAAGTCCC -3'

Posted On

2017-10-10