Mutagenetix > Incidental Mutation

Incidental Mutation 'R6163:Or7e169'

489979

Institutional Source

Beutler Lab

Gene Symbol

Or7e169

Ensembl Gene

ENSMUSG00000066905

Gene Name

olfactory receptor family 7 subfamily E member 169

Synonyms

GA_x6K02T2PVTD-13586614-13585661, Olfr860, MOR146-2

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R6163 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19756919-19761043 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 19757024 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 297 (I297T)

Ref Sequence

ENSEMBL: ENSMUSP00000148568 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086482] [ENSMUST00000211924] [ENSMUST00000212353]

AlphaFold

Q8VFF7

Predicted Effect

probably benign
Transcript: ENSMUST00000086482
AA Change: I297T

PolyPhen 2 Score 0.447 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000130735
Gene: ENSMUSG00000066905
AA Change: I297T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	3.5e-56	PFAM
Pfam:7TM_GPCR_Srsx	35	305	1.1e-8	PFAM
Pfam:7tm_1	41	290	9.3e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000211924

Predicted Effect

probably benign
Transcript: ENSMUST00000212353
AA Change: I297T

PolyPhen 2 Score 0.447 (Sensitivity: 0.89; Specificity: 0.90)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212525

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212838

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 94.5%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700018F24Rik	C	T	5: 144,982,098 (GRCm39)	Q228*	probably null	Het
Asic5	A	G	3: 81,913,833 (GRCm39)	N169S	probably damaging	Het
Atad2b	T	A	12: 5,004,593 (GRCm39)	L374H	probably benign	Het
Birc2	G	A	9: 7,819,036 (GRCm39)	T544I	probably benign	Het
Blm	GCCTCCTCCTCCTCCTCCTCCTCCTCCTCC	GCCTCCTCCTCCTCCTCCTCCTCCTCC	7: 80,162,652 (GRCm39)		probably benign	Het
Cc2d2b	C	T	19: 40,744,950 (GRCm39)	T23I	probably benign	Het
Ccdc137	G	T	11: 120,350,927 (GRCm39)	R108L	possibly damaging	Het
Cenpe	T	C	3: 134,974,764 (GRCm39)	I2308T	probably damaging	Het
Clec2m	C	T	6: 129,303,710 (GRCm39)	R85H	probably benign	Het
Cpt1b	T	C	15: 89,308,620 (GRCm39)	T103A	probably benign	Het
Cpvl	C	A	6: 53,850,503 (GRCm39)	V445F	probably damaging	Het
Cttnbp2	A	G	6: 18,434,950 (GRCm39)	S303P	possibly damaging	Het
Cyp17a1	T	A	19: 46,657,761 (GRCm39)	I264F	possibly damaging	Het
Cyp51	T	A	5: 4,150,199 (GRCm39)	I175F	probably damaging	Het
Dennd4c	C	T	4: 86,723,828 (GRCm39)	P695S	possibly damaging	Het
Dlgap2	A	T	8: 14,896,641 (GRCm39)	Q1039L	probably damaging	Het
Dnah14	T	C	1: 181,493,926 (GRCm39)	L1694P	probably benign	Het
Dnah2	G	A	11: 69,411,729 (GRCm39)	Q298*	probably null	Het
Dsg2	G	T	18: 20,731,726 (GRCm39)		probably null	Het
Esr2	A	C	12: 76,168,643 (GRCm39)	V522G	probably damaging	Het
Fmo9	T	A	1: 166,494,962 (GRCm39)	H262L	probably benign	Het
Glb1l	T	A	1: 75,178,051 (GRCm39)	M373L	probably benign	Het
Gls	A	G	1: 52,254,735 (GRCm39)	S247P	probably benign	Het
Gm37240	T	A	3: 84,423,092 (GRCm39)	E213D	probably damaging	Het
Gmip	T	A	8: 70,270,022 (GRCm39)	V675E	probably benign	Het
Grb10	C	A	11: 11,893,932 (GRCm39)	E330*	probably null	Het
H2ac13	A	T	13: 21,900,859 (GRCm39)	N90Y	probably damaging	Het
Hivep2	A	G	10: 14,005,736 (GRCm39)	K778R	probably damaging	Het
Ighv1-26	T	C	12: 114,752,416 (GRCm39)	S4G	probably benign	Het
Igkv3-3	T	A	6: 70,664,257 (GRCm39)	V33E	possibly damaging	Het
Itga2	C	A	13: 115,002,726 (GRCm39)	G588V	probably damaging	Het
Itpr1	C	T	6: 108,365,245 (GRCm39)	H58Y	probably damaging	Het
Jmjd1c	T	A	10: 67,083,827 (GRCm39)	D2365E	possibly damaging	Het
Limk1	T	C	5: 134,686,809 (GRCm39)	Y518C	probably damaging	Het
Marchf6	G	C	15: 31,465,497 (GRCm39)	H802Q	probably benign	Het
Mark2	A	G	19: 7,268,126 (GRCm39)	S26P	probably benign	Het
Mdn1	T	A	4: 32,716,040 (GRCm39)	L2074Q	probably damaging	Het
Mrpl37	T	C	4: 106,921,793 (GRCm39)	E174G	possibly damaging	Het
Mtcl1	T	C	17: 66,686,326 (GRCm39)	H860R	probably benign	Het
Neurod1	A	G	2: 79,284,505 (GRCm39)	F293L	probably benign	Het
Nwd1	C	T	8: 73,388,814 (GRCm39)	R81W	probably damaging	Het
Nwd2	C	A	5: 63,963,131 (GRCm39)	A905E	probably benign	Het
Or14a260	A	T	7: 85,985,592 (GRCm39)	I4N	possibly damaging	Het
Or1x6	T	A	11: 50,939,595 (GRCm39)	Y220*	probably null	Het
Or5b12b	A	C	19: 12,861,472 (GRCm39)	T76P	probably damaging	Het
Otop1	C	A	5: 38,445,234 (GRCm39)		probably null	Het
Otp	A	G	13: 95,012,288 (GRCm39)	H4R	probably damaging	Het
P4htm	A	C	9: 108,459,150 (GRCm39)	Y261D	probably damaging	Het
Pcsk5	A	T	19: 17,450,405 (GRCm39)	C1148S	probably damaging	Het
Plekhg1	A	T	10: 3,914,369 (GRCm39)	R1419W	probably damaging	Het
Pramel20	A	G	4: 143,298,605 (GRCm39)	K183E	probably damaging	Het
Prkd3	C	A	17: 79,273,784 (GRCm39)	D491Y	possibly damaging	Het
Prr11	A	C	11: 86,994,454 (GRCm39)	L64R	possibly damaging	Het
Pygo1	G	T	9: 72,851,980 (GRCm39)	A56S	probably damaging	Het
Rexo5	A	G	7: 119,404,470 (GRCm39)	T189A	probably damaging	Het
Rnf213	A	T	11: 119,349,254 (GRCm39)	H3784L	possibly damaging	Het
Rps6ka5	T	G	12: 100,562,179 (GRCm39)		probably null	Het
Slco2b1	T	A	7: 99,338,106 (GRCm39)	I93F	probably damaging	Het
Slfn8	T	C	11: 82,894,690 (GRCm39)	*408W	probably null	Het
Sptbn1	C	A	11: 30,109,443 (GRCm39)	E51*	probably null	Het
Ssc5d	T	A	7: 4,930,253 (GRCm39)	H111Q	probably damaging	Het
Sult1c2	T	C	17: 54,280,981 (GRCm39)	N41D	probably benign	Het
Taar8c	G	A	10: 23,977,116 (GRCm39)	T232I	probably benign	Het
Tdo2	T	C	3: 81,882,710 (GRCm39)	E2G	possibly damaging	Het
Tff2	T	C	17: 31,363,152 (GRCm39)	E24G	probably benign	Het
Tjp2	C	A	19: 24,103,068 (GRCm39)		probably null	Het
Tnfrsf1b	A	T	4: 144,946,477 (GRCm39)	D311E	probably benign	Het
Usp15	G	T	10: 123,004,210 (GRCm39)	N181K	probably damaging	Het
Vmn1r175	T	G	7: 23,508,591 (GRCm39)	E12A	possibly damaging	Het
Vmn1r47	T	C	6: 89,999,773 (GRCm39)	S302P	probably damaging	Het
Vmn2r58	A	G	7: 41,486,825 (GRCm39)	M690T	probably benign	Het
Wee1	T	A	7: 109,734,858 (GRCm39)	H465Q	probably damaging	Het
Zfr	C	T	15: 12,146,331 (GRCm39)	A294V	unknown	Het

Other mutations in Or7e169

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00942:Or7e169	APN	9	19,757,555 (GRCm39)	missense	probably damaging	1.00
IGL02216:Or7e169	APN	9	19,757,861 (GRCm39)	missense	probably damaging	0.99
IGL02269:Or7e169	APN	9	19,757,024 (GRCm39)	missense	possibly damaging	0.85
IGL02964:Or7e169	APN	9	19,757,550 (GRCm39)	nonsense	probably null
R0042:Or7e169	UTSW	9	19,757,075 (GRCm39)	missense	probably benign
R1505:Or7e169	UTSW	9	19,757,084 (GRCm39)	missense	probably benign	0.39
R1941:Or7e169	UTSW	9	19,757,246 (GRCm39)	missense	probably damaging	0.99
R2030:Or7e169	UTSW	9	19,757,709 (GRCm39)	missense	probably benign	0.30
R3150:Or7e169	UTSW	9	19,757,510 (GRCm39)	missense	possibly damaging	0.80
R4597:Or7e169	UTSW	9	19,756,987 (GRCm39)	missense	probably benign	0.01
R5004:Or7e169	UTSW	9	19,757,398 (GRCm39)	missense	probably benign	0.00
R5006:Or7e169	UTSW	9	19,757,567 (GRCm39)	missense	probably benign	0.33
R5350:Or7e169	UTSW	9	19,757,912 (GRCm39)	start codon destroyed	probably null	0.97
R6368:Or7e169	UTSW	9	19,757,705 (GRCm39)	missense	probably damaging	1.00
R7206:Or7e169	UTSW	9	19,757,856 (GRCm39)	missense	probably damaging	0.99
R7315:Or7e169	UTSW	9	19,757,131 (GRCm39)	missense	probably damaging	0.99
R8195:Or7e169	UTSW	9	19,757,780 (GRCm39)	missense	probably damaging	0.99
R8537:Or7e169	UTSW	9	19,757,848 (GRCm39)	missense	probably damaging	0.96
R8546:Or7e169	UTSW	9	19,757,685 (GRCm39)	missense	probably damaging	0.99
R8803:Or7e169	UTSW	9	19,757,462 (GRCm39)	missense	possibly damaging	0.95
R9049:Or7e169	UTSW	9	19,757,045 (GRCm39)	missense	probably damaging	1.00
R9164:Or7e169	UTSW	9	19,757,504 (GRCm39)	missense	possibly damaging	0.73
R9254:Or7e169	UTSW	9	19,757,212 (GRCm39)	missense	possibly damaging	0.83
R9379:Or7e169	UTSW	9	19,757,212 (GRCm39)	missense	possibly damaging	0.83
R9513:Or7e169	UTSW	9	19,757,816 (GRCm39)	missense	possibly damaging	0.73
R9515:Or7e169	UTSW	9	19,757,816 (GRCm39)	missense	possibly damaging	0.73

Predicted Primers

PCR Primer
(F):5'- ACAGGCACAGCAAGATACATTTAG -3'
(R):5'- GGGAAATGTAAAGCGTTCTCTAC -3'

Sequencing Primer
(F):5'- GCACAGCAAGATACATTTAGATTCAC -3'
(R):5'- AAAGCGTTCTCTACTTGTGGATC -3'

Posted On

2017-10-10