Incidental Mutation 'R6169:Tcte1'
ID 490347
Institutional Source Beutler Lab
Gene Symbol Tcte1
Ensembl Gene ENSMUSG00000023949
Gene Name t-complex-associated testis expressed 1
Synonyms D17Sil1, Tcte-1
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6169 (G1)
Quality Score 225.009
Status Validated
Chromosome 17
Chromosomal Location 45834360-45853605 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 45845996 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Methionine to Lysine at position 200 (M200K)
Ref Sequence ENSEMBL: ENSMUSP00000109175 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000113547]
AlphaFold A6H639
Predicted Effect probably benign
Transcript: ENSMUST00000113547
AA Change: M200K

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)
SMART Domains Protein: ENSMUSP00000109175
Gene: ENSMUSG00000023949
AA Change: M200K

DomainStartEndE-ValueType
low complexity region 6 17 N/A INTRINSIC
low complexity region 28 47 N/A INTRINSIC
low complexity region 206 222 N/A INTRINSIC
Blast:LRR 235 274 7e-14 BLAST
LRR 304 331 5.02e-6 SMART
LRR 332 358 1.28e-3 SMART
LRR 359 386 5.81e-2 SMART
LRR 387 414 2.05e-2 SMART
LRR 415 442 1.13e-4 SMART
Blast:LRR 443 470 3e-8 BLAST
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.1%
  • 20x: 94.5%
Validation Efficiency 94% (67/71)
Allele List at MGI
Other mutations in this stock
Total: 70 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930556J24Rik C A 11: 3,888,005 (GRCm39) C127F unknown Het
9930111J21Rik2 A T 11: 48,910,088 (GRCm39) probably null Het
Adamts16 A T 13: 70,918,393 (GRCm39) L676* probably null Het
Adgrv1 A T 13: 81,567,378 (GRCm39) V5265E probably benign Het
Afg3l2 G T 18: 67,554,329 (GRCm39) L458M probably damaging Het
Akap6 T A 12: 53,189,141 (GRCm39) M2185K probably benign Het
Ankfy1 G A 11: 72,645,285 (GRCm39) C788Y probably benign Het
Ankrd50 A G 3: 38,509,988 (GRCm39) V793A probably damaging Het
Arid2 T A 15: 96,266,558 (GRCm39) N535K probably benign Het
Atrnl1 A G 19: 57,630,895 (GRCm39) T221A probably benign Het
Bbof1 C T 12: 84,473,588 (GRCm39) T306I probably benign Het
Cabs1 C T 5: 88,127,613 (GRCm39) T88I possibly damaging Het
Ccdc60 C A 5: 116,275,131 (GRCm39) A351S probably benign Het
Cetn3 A C 13: 81,940,097 (GRCm39) R44S probably damaging Het
Cluap1 A G 16: 3,746,425 (GRCm39) K262R possibly damaging Het
Cnrip1 G A 11: 17,004,731 (GRCm39) V94I probably null Het
Cog6 A T 3: 52,914,722 (GRCm39) S245T probably benign Het
Disp2 T A 2: 118,622,031 (GRCm39) V921D probably damaging Het
Entrep2 G A 7: 64,409,147 (GRCm39) P416S probably benign Het
Ep400 T C 5: 110,889,863 (GRCm39) K504E possibly damaging Het
Fbn1 T A 2: 125,177,409 (GRCm39) probably null Het
Fyb2 T C 4: 104,857,713 (GRCm39) V630A probably benign Het
Gm43218 T A 6: 70,217,606 (GRCm39) Y50F probably benign Het
Gm5431 T C 11: 48,779,402 (GRCm39) T507A probably benign Het
Gucy2e G T 11: 69,126,930 (GRCm39) A181E probably benign Het
Hrnr C G 3: 93,233,062 (GRCm39) S1100* probably null Het
Htt T C 5: 35,064,817 (GRCm39) V3010A probably damaging Het
Ighv1-11 T C 12: 114,575,918 (GRCm39) Y99C probably damaging Het
Ighv1-12 T C 12: 114,579,577 (GRCm39) K25E possibly damaging Het
Il6ra A G 3: 89,778,598 (GRCm39) F417S probably benign Het
Itgb4 T C 11: 115,885,102 (GRCm39) S994P probably damaging Het
Itgbl1 G A 14: 123,897,790 (GRCm39) A24T probably benign Het
Itpr1 T C 6: 108,346,077 (GRCm39) F127L probably damaging Het
Itprid2 T G 2: 79,475,406 (GRCm39) I455R probably damaging Het
Krt86 A G 15: 101,374,170 (GRCm39) Y243C probably damaging Het
Lnx1 A T 5: 74,838,230 (GRCm39) W11R probably damaging Het
Mast4 A G 13: 102,923,929 (GRCm39) L302P probably damaging Het
Mettl3 A G 14: 52,536,214 (GRCm39) V210A possibly damaging Het
Mllt1 T C 17: 57,206,822 (GRCm39) T341A probably benign Het
Obscn T C 11: 58,891,325 (GRCm39) E7069G unknown Het
Or1e27-ps1 A T 11: 73,556,160 (GRCm39) T242S possibly damaging Het
Or52b4 T C 7: 102,184,479 (GRCm39) I175T probably benign Het
Osgep A G 14: 51,157,209 (GRCm39) V11A possibly damaging Het
Oxld1 G A 11: 120,347,675 (GRCm39) A174V possibly damaging Het
Pibf1 A T 14: 99,350,443 (GRCm39) E197V probably null Het
Pkn1 G A 8: 84,407,835 (GRCm39) Q425* probably null Het
Pramel22 T C 4: 143,380,685 (GRCm39) Y446C probably benign Het
Prss35 T C 9: 86,637,491 (GRCm39) I87T probably benign Het
Psmc3 T C 2: 90,888,184 (GRCm39) F304S probably damaging Het
Psmd11 T A 11: 80,351,539 (GRCm39) M254K probably damaging Het
Ralgapa2 T C 2: 146,292,385 (GRCm39) Y218C probably damaging Het
Sec23b C T 2: 144,428,894 (GRCm39) R701C probably damaging Het
Slc22a21 A G 11: 53,848,913 (GRCm39) S280P probably damaging Het
Slc39a12 T C 2: 14,405,044 (GRCm39) I212T possibly damaging Het
Slc7a12 T C 3: 14,562,388 (GRCm39) V255A probably damaging Het
Smc3 T A 19: 53,622,517 (GRCm39) N697K probably benign Het
Snai1 C A 2: 167,380,831 (GRCm39) P108Q probably benign Het
Stim2 T G 5: 54,276,021 (GRCm39) L732R probably damaging Het
Syde1 A G 10: 78,421,938 (GRCm39) L597S probably damaging Het
Tenm2 T A 11: 36,030,517 (GRCm39) T761S probably damaging Het
Thoc2l T A 5: 104,666,262 (GRCm39) N261K probably benign Het
Tlr2 C A 3: 83,745,455 (GRCm39) E209D probably benign Het
Tpp2 T C 1: 44,022,739 (GRCm39) L33P probably damaging Het
Trmt1l C A 1: 151,304,704 (GRCm39) probably benign Het
Unc45a A C 7: 79,978,511 (GRCm39) S646A possibly damaging Het
Usp37 A T 1: 74,534,910 (GRCm39) I12N probably damaging Het
Vmn2r81 T C 10: 79,104,382 (GRCm39) V335A probably benign Het
Wwc2 A T 8: 48,311,878 (GRCm39) S762T unknown Het
Yeats2 A G 16: 20,038,417 (GRCm39) K129E probably damaging Het
Zscan4-ps2 T C 7: 11,251,558 (GRCm39) V198A probably benign Het
Other mutations in Tcte1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00094:Tcte1 APN 17 45,845,854 (GRCm39) missense probably damaging 1.00
IGL00945:Tcte1 APN 17 45,852,115 (GRCm39) missense probably benign 0.15
IGL01120:Tcte1 APN 17 45,850,594 (GRCm39) missense probably damaging 0.99
IGL01132:Tcte1 APN 17 45,850,788 (GRCm39) missense possibly damaging 0.93
IGL01884:Tcte1 APN 17 45,850,735 (GRCm39) splice site probably null
IGL02418:Tcte1 APN 17 45,852,128 (GRCm39) missense probably benign 0.37
IGL02731:Tcte1 APN 17 45,850,812 (GRCm39) missense probably benign 0.00
IGL03130:Tcte1 APN 17 45,844,222 (GRCm39) missense probably damaging 1.00
R0358:Tcte1 UTSW 17 45,846,211 (GRCm39) missense probably benign 0.34
R1519:Tcte1 UTSW 17 45,846,178 (GRCm39) missense probably damaging 1.00
R2013:Tcte1 UTSW 17 45,852,237 (GRCm39) missense probably benign 0.30
R2014:Tcte1 UTSW 17 45,852,237 (GRCm39) missense probably benign 0.30
R3744:Tcte1 UTSW 17 45,850,597 (GRCm39) missense probably damaging 0.99
R4250:Tcte1 UTSW 17 45,850,617 (GRCm39) missense probably benign 0.04
R4976:Tcte1 UTSW 17 45,845,854 (GRCm39) missense probably damaging 1.00
R5398:Tcte1 UTSW 17 45,850,752 (GRCm39) nonsense probably null
R6251:Tcte1 UTSW 17 45,846,085 (GRCm39) missense probably benign
R6279:Tcte1 UTSW 17 45,844,215 (GRCm39) missense possibly damaging 0.69
R6300:Tcte1 UTSW 17 45,844,215 (GRCm39) missense possibly damaging 0.69
R6316:Tcte1 UTSW 17 45,845,786 (GRCm39) missense probably benign
R6417:Tcte1 UTSW 17 45,846,056 (GRCm39) missense probably damaging 0.97
R6892:Tcte1 UTSW 17 45,844,083 (GRCm39) missense probably benign
R7047:Tcte1 UTSW 17 45,844,294 (GRCm39) missense possibly damaging 0.52
R7645:Tcte1 UTSW 17 45,845,915 (GRCm39) missense probably benign 0.00
R8125:Tcte1 UTSW 17 45,850,618 (GRCm39) missense possibly damaging 0.88
R9710:Tcte1 UTSW 17 45,850,798 (GRCm39) missense possibly damaging 0.94
Z1176:Tcte1 UTSW 17 45,845,997 (GRCm39) missense probably benign
Z1177:Tcte1 UTSW 17 45,845,938 (GRCm39) missense possibly damaging 0.93
Predicted Primers PCR Primer
(F):5'- CGATGAGAATTACTGGCACCG -3'
(R):5'- GGTTCCACTCAAAATTCATGCC -3'

Sequencing Primer
(F):5'- AATTACTGGCACCGCTGCTG -3'
(R):5'- AAATTCATGCCACAGTCTTTGACGC -3'
Posted On 2017-10-10