Mutagenetix > Incidental Mutation

Incidental Mutation 'R0529:Vmn2r12'

49043

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r12

Ensembl Gene

ENSMUSG00000090688

Gene Name

vomeronasal 2, receptor 12

Synonyms

Gm6769

MMRRC Submission

038721-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R0529 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109233715-109245730 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 109240714 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 133 (V133A)

Ref Sequence

ENSEMBL: ENSMUSP00000093612 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095922]

AlphaFold

L7N217

Predicted Effect

probably benign
Transcript: ENSMUST00000095922
AA Change: V133A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000093612
Gene: ENSMUSG00000090688
AA Change: V133A

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.8e-30	PFAM
Pfam:NCD3G	505	559	1.7e-18	PFAM
Pfam:7tm_3	591	827	3.9e-54	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.5%
20x: 93.3%

Validation Efficiency

97% (58/60)

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010I14Rik	G	A	17: 9,211,228 (GRCm39)	V126I	probably benign	Het
Aasdh	A	C	5: 77,024,114 (GRCm39)	Y179*	probably null	Het
Afp	A	G	5: 90,652,254 (GRCm39)	Y415C	probably damaging	Het
Aldh5a1	G	T	13: 25,097,856 (GRCm39)	T393K	probably benign	Het
Arhgef26	T	C	3: 62,247,146 (GRCm39)	S77P	probably benign	Het
Axl	A	G	7: 25,486,712 (GRCm39)		probably benign	Het
Card10	A	G	15: 78,664,675 (GRCm39)		probably null	Het
Ccdc71l	G	A	12: 32,429,251 (GRCm39)	S90N	probably damaging	Het
Cebpa	A	T	7: 34,819,624 (GRCm39)	T261S	probably benign	Het
Cnmd	T	C	14: 79,879,481 (GRCm39)	E219G	probably benign	Het
Cntln	T	A	4: 84,986,062 (GRCm39)	L1010H	probably damaging	Het
Cul9	A	G	17: 46,831,394 (GRCm39)		probably benign	Het
Cyld	A	G	8: 89,456,387 (GRCm39)	E479G	probably benign	Het
Dmp1	A	G	5: 104,360,092 (GRCm39)	E256G	probably benign	Het
Dnmt1	T	C	9: 20,822,846 (GRCm39)	D1140G	probably damaging	Het
Drd2	A	C	9: 49,318,374 (GRCm39)	M439L	probably benign	Het
Drd3	G	A	16: 43,643,077 (GRCm39)	V438M	probably damaging	Het
Dyrk3	A	G	1: 131,057,858 (GRCm39)	I70T	probably benign	Het
Fbxo38	T	C	18: 62,639,057 (GRCm39)	K1082E	probably damaging	Het
Fbxw10	A	C	11: 62,750,671 (GRCm39)	D428A	probably damaging	Het
Fmn1	T	A	2: 113,538,198 (GRCm39)		probably benign	Het
Fmnl2	A	T	2: 52,932,377 (GRCm39)	I119F	probably damaging	Het
Frmd4a	A	G	2: 4,610,834 (GRCm39)	T995A	probably damaging	Het
Gda	T	A	19: 21,402,901 (GRCm39)	I82F	probably damaging	Het
Gpatch4	T	A	3: 87,958,583 (GRCm39)	H22Q	probably damaging	Het
Gpr55	A	G	1: 85,869,225 (GRCm39)	F119L	probably benign	Het
Gtf2i	A	T	5: 134,290,723 (GRCm39)	L425*	probably null	Het
Knstrn	T	A	2: 118,661,461 (GRCm39)		probably benign	Het
Lipo2	T	A	19: 33,724,335 (GRCm39)	I144L	probably benign	Het
Lrp1	T	C	10: 127,377,463 (GRCm39)		probably null	Het
Mtmr14	T	C	6: 113,243,213 (GRCm39)		probably benign	Het
Nsmce4a	A	T	7: 130,135,536 (GRCm39)	S345R	probably benign	Het
Oacyl	T	A	18: 65,875,290 (GRCm39)	V385D	probably damaging	Het
Or6k8-ps1	G	A	1: 173,979,696 (GRCm39)	A205T	probably benign	Het
Or8b52	A	T	9: 38,576,808 (GRCm39)	C111S	probably benign	Het
Phlpp2	T	C	8: 110,603,603 (GRCm39)	S55P	probably benign	Het
Pkhd1l1	T	A	15: 44,390,150 (GRCm39)	V1422E	possibly damaging	Het
Plcd3	T	G	11: 102,971,013 (GRCm39)	H181P	probably benign	Het
Psmc5	G	A	11: 106,151,990 (GRCm39)		probably null	Het
Psmd11	T	C	11: 80,361,515 (GRCm39)		probably benign	Het
Rab39	T	C	9: 53,598,016 (GRCm39)	Y83C	probably damaging	Het
Ric8a	A	G	7: 140,440,806 (GRCm39)	E93G	probably damaging	Het
Rtp3	T	C	9: 110,816,152 (GRCm39)	E133G	possibly damaging	Het
Serpina1e	A	C	12: 103,915,363 (GRCm39)	L281R	probably damaging	Het
Slc35e1	T	C	8: 73,246,415 (GRCm39)		probably benign	Het
Tent5c	A	G	3: 100,379,686 (GRCm39)	Y357H	probably benign	Het
Tmem63a	A	T	1: 180,788,659 (GRCm39)	E332V	probably benign	Het
Tnk1	T	C	11: 69,745,990 (GRCm39)	T312A	probably damaging	Het
Traf3ip3	G	T	1: 192,877,119 (GRCm39)		probably benign	Het
Trappc11	A	G	8: 47,980,014 (GRCm39)	V174A	possibly damaging	Het
Vmn1r174	G	A	7: 23,453,622 (GRCm39)	R96H	probably benign	Het
Vmn1r7	T	A	6: 57,001,450 (GRCm39)	Y270F	possibly damaging	Het
Vmn2r18	T	A	5: 151,485,988 (GRCm39)	E502V	probably damaging	Het
Wipf3	C	A	6: 54,462,348 (GRCm39)	P186Q	probably damaging	Het
Yipf5	A	T	18: 40,345,215 (GRCm39)	M55K	probably benign	Het
Zbtb7a	G	A	10: 80,979,820 (GRCm39)	V5M	probably damaging	Het
Zfy1	G	T	Y: 726,040 (GRCm39)	S575Y	probably damaging	Het

Other mutations in Vmn2r12

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00948:Vmn2r12	APN	5	109,245,541 (GRCm39)	missense	possibly damaging	0.47
IGL01096:Vmn2r12	APN	5	109,234,125 (GRCm39)	missense	probably damaging	1.00
IGL01538:Vmn2r12	APN	5	109,239,716 (GRCm39)	missense	probably damaging	1.00
IGL01548:Vmn2r12	APN	5	109,240,893 (GRCm39)	nonsense	probably null
IGL01762:Vmn2r12	APN	5	109,234,430 (GRCm39)	missense	probably damaging	0.99
IGL01860:Vmn2r12	APN	5	109,240,025 (GRCm39)	missense	probably benign	0.10
IGL02269:Vmn2r12	APN	5	109,234,343 (GRCm39)	missense	probably damaging	1.00
IGL02530:Vmn2r12	APN	5	109,233,858 (GRCm39)	missense	probably damaging	1.00
IGL02887:Vmn2r12	APN	5	109,238,351 (GRCm39)	missense	probably benign	0.03
IGL03265:Vmn2r12	APN	5	109,239,936 (GRCm39)	missense	probably benign	0.05
R0396:Vmn2r12	UTSW	5	109,240,765 (GRCm39)	missense	probably benign	0.00
R0497:Vmn2r12	UTSW	5	109,239,755 (GRCm39)	nonsense	probably null
R0715:Vmn2r12	UTSW	5	109,238,373 (GRCm39)	missense	probably benign	0.10
R0742:Vmn2r12	UTSW	5	109,234,281 (GRCm39)	missense	possibly damaging	0.55
R0894:Vmn2r12	UTSW	5	109,235,716 (GRCm39)	critical splice donor site	probably null
R1173:Vmn2r12	UTSW	5	109,240,720 (GRCm39)	missense	probably benign	0.00
R1174:Vmn2r12	UTSW	5	109,240,720 (GRCm39)	missense	probably benign	0.00
R1259:Vmn2r12	UTSW	5	109,239,763 (GRCm39)	missense	probably damaging	0.97
R1349:Vmn2r12	UTSW	5	109,234,452 (GRCm39)	missense	probably benign	0.00
R1388:Vmn2r12	UTSW	5	109,240,840 (GRCm39)	missense	possibly damaging	0.56
R1549:Vmn2r12	UTSW	5	109,240,696 (GRCm39)	missense	probably benign	0.06
R1766:Vmn2r12	UTSW	5	109,239,910 (GRCm39)	missense	probably damaging	1.00
R1781:Vmn2r12	UTSW	5	109,239,594 (GRCm39)	missense	probably benign	0.00
R1885:Vmn2r12	UTSW	5	109,239,942 (GRCm39)	missense	probably damaging	1.00
R2159:Vmn2r12	UTSW	5	109,239,340 (GRCm39)	missense	probably benign	0.02
R2420:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2421:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2422:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2937:Vmn2r12	UTSW	5	109,239,397 (GRCm39)	missense	probably damaging	1.00
R2938:Vmn2r12	UTSW	5	109,239,397 (GRCm39)	missense	probably damaging	1.00
R3898:Vmn2r12	UTSW	5	109,238,370 (GRCm39)	missense	probably benign	0.02
R4061:Vmn2r12	UTSW	5	109,240,058 (GRCm39)	missense	possibly damaging	0.95
R4063:Vmn2r12	UTSW	5	109,240,058 (GRCm39)	missense	possibly damaging	0.95
R4090:Vmn2r12	UTSW	5	109,239,412 (GRCm39)	missense	probably benign	0.06
R4297:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4298:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4299:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4304:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4306:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4307:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4308:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4594:Vmn2r12	UTSW	5	109,234,301 (GRCm39)	missense	probably damaging	1.00
R4783:Vmn2r12	UTSW	5	109,234,379 (GRCm39)	missense	probably damaging	1.00
R4900:Vmn2r12	UTSW	5	109,240,852 (GRCm39)	missense	probably damaging	1.00
R4929:Vmn2r12	UTSW	5	109,239,544 (GRCm39)	missense	probably damaging	1.00
R4974:Vmn2r12	UTSW	5	109,239,372 (GRCm39)	missense	probably damaging	1.00
R5389:Vmn2r12	UTSW	5	109,238,261 (GRCm39)	missense	probably benign	0.00
R5431:Vmn2r12	UTSW	5	109,239,684 (GRCm39)	missense	probably damaging	0.99
R5527:Vmn2r12	UTSW	5	109,234,483 (GRCm39)	nonsense	probably null
R5639:Vmn2r12	UTSW	5	109,240,666 (GRCm39)	missense	probably benign	0.06
R5753:Vmn2r12	UTSW	5	109,239,670 (GRCm39)	missense	probably damaging	1.00
R5797:Vmn2r12	UTSW	5	109,233,736 (GRCm39)	nonsense	probably null
R6142:Vmn2r12	UTSW	5	109,240,763 (GRCm39)	missense	probably benign
R6162:Vmn2r12	UTSW	5	109,234,430 (GRCm39)	missense	probably damaging	0.99
R6176:Vmn2r12	UTSW	5	109,233,866 (GRCm39)	missense	probably benign	0.43
R6853:Vmn2r12	UTSW	5	109,240,771 (GRCm39)	missense	probably damaging	1.00
R7238:Vmn2r12	UTSW	5	109,245,655 (GRCm39)	missense	possibly damaging	0.81
R7341:Vmn2r12	UTSW	5	109,239,811 (GRCm39)	missense	possibly damaging	0.95
R7341:Vmn2r12	UTSW	5	109,234,113 (GRCm39)	missense	possibly damaging	0.74
R7383:Vmn2r12	UTSW	5	109,240,684 (GRCm39)	missense	probably benign	0.19
R7740:Vmn2r12	UTSW	5	109,239,615 (GRCm39)	missense	probably damaging	1.00
R7749:Vmn2r12	UTSW	5	109,233,920 (GRCm39)	missense	probably damaging	0.99
R7861:Vmn2r12	UTSW	5	109,235,829 (GRCm39)	missense	probably benign	0.00
R7908:Vmn2r12	UTSW	5	109,234,307 (GRCm39)	missense	probably damaging	1.00
R8128:Vmn2r12	UTSW	5	109,239,747 (GRCm39)	missense	possibly damaging	0.81
R8175:Vmn2r12	UTSW	5	109,238,349 (GRCm39)	missense	probably damaging	0.97
R8234:Vmn2r12	UTSW	5	109,234,074 (GRCm39)	missense	probably benign	0.01
R8771:Vmn2r12	UTSW	5	109,239,952 (GRCm39)	missense	possibly damaging	0.95
R8947:Vmn2r12	UTSW	5	109,234,522 (GRCm39)	missense	possibly damaging	0.64
R8991:Vmn2r12	UTSW	5	109,234,033 (GRCm39)	nonsense	probably null
R9116:Vmn2r12	UTSW	5	109,233,885 (GRCm39)	missense	probably damaging	1.00
R9122:Vmn2r12	UTSW	5	109,240,910 (GRCm39)	missense	probably benign	0.00
R9153:Vmn2r12	UTSW	5	109,234,203 (GRCm39)	missense	probably damaging	1.00
R9371:Vmn2r12	UTSW	5	109,234,452 (GRCm39)	missense	probably benign	0.00
R9375:Vmn2r12	UTSW	5	109,233,986 (GRCm39)	missense	probably damaging	1.00
R9524:Vmn2r12	UTSW	5	109,239,823 (GRCm39)	missense	probably damaging	1.00
R9587:Vmn2r12	UTSW	5	109,239,322 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r12	UTSW	5	109,240,646 (GRCm39)	missense	probably benign
Z1176:Vmn2r12	UTSW	5	109,239,303 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TTGTTAACTTgggctatcgagctgg -3'
(R):5'- TGGCAGCACTCTGACTCTCCAC -3'

Sequencing Primer
(F):5'- tcctaaattcaattcccaataaccac -3'
(R):5'- CTAGTTCAAGGTTAGCATTCAGAG -3'

Posted On

2013-06-12