Mutagenetix > Incidental Mutation

Incidental Mutation 'R1574:Zfp653'

500343

Institutional Source

Beutler Lab

Gene Symbol

Zfp653

Ensembl Gene

ENSMUSG00000038895

Gene Name

zinc finger protein 653

Synonyms

E430039K05Rik

MMRRC Submission

045014-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.307) question?

Stock #

R1574 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

21966707-21982672 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 21969274 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 331 (E331*)

Ref Sequence

ENSEMBL: ENSMUSP00000137064 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043922] [ENSMUST00000179605]

AlphaFold

Q6YND2

Predicted Effect

probably null
Transcript: ENSMUST00000043922
AA Change: E331*

SMART Domains

Protein: ENSMUSP00000045895
Gene: ENSMUSG00000038895
AA Change: E331*

Domain	Start	End	E-Value	Type
AT_hook	29	41	2.28e0	SMART
low complexity region	105	116	N/A	INTRINSIC
low complexity region	192	205	N/A	INTRINSIC
low complexity region	209	232	N/A	INTRINSIC
low complexity region	443	456	N/A	INTRINSIC
ZnF_C2H2	467	492	4.11e-2	SMART
ZnF_C2H2	498	522	4.47e-3	SMART
ZnF_C2H2	528	550	4.87e-4	SMART
ZnF_C2H2	556	578	2.99e-4	SMART
ZnF_C2H2	586	609	1.31e0	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000179605
AA Change: E331*

SMART Domains

Protein: ENSMUSP00000137064
Gene: ENSMUSG00000038895
AA Change: E331*

Domain	Start	End	E-Value	Type
AT_hook	29	41	2.28e0	SMART
low complexity region	105	116	N/A	INTRINSIC
low complexity region	192	205	N/A	INTRINSIC
low complexity region	209	232	N/A	INTRINSIC
low complexity region	451	464	N/A	INTRINSIC
ZnF_C2H2	475	500	4.11e-2	SMART
ZnF_C2H2	506	530	4.47e-3	SMART
ZnF_C2H2	536	558	4.87e-4	SMART
ZnF_C2H2	564	586	2.99e-4	SMART
ZnF_C2H2	594	617	1.31e0	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000213738

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216916

Coding Region Coverage

1x: 98.3%
3x: 96.3%
10x: 84.0%
20x: 52.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrl3	T	A	5: 81,935,296 (GRCm39)	N1276K	probably damaging	Het
Als2cl	A	G	9: 110,713,128 (GRCm39)	E6G	probably damaging	Het
Ankrd12	A	T	17: 66,293,269 (GRCm39)	D721E	probably benign	Het
Anpep	A	G	7: 79,488,155 (GRCm39)		probably null	Het
Apob	A	T	12: 8,040,839 (GRCm39)	I655L	possibly damaging	Het
Atp2b1	T	A	10: 98,832,810 (GRCm39)	L437Q	probably damaging	Het
Cacna2d3	T	A	14: 29,073,779 (GRCm39)	R222S	probably damaging	Het
Cenpf	C	T	1: 189,384,910 (GRCm39)	D2457N	probably damaging	Het
Cenpo	A	T	12: 4,265,433 (GRCm39)		probably null	Het
Ces2b	G	T	8: 105,562,521 (GRCm39)	A284S	probably benign	Het
Clock	T	C	5: 76,390,679 (GRCm39)	D311G	probably damaging	Het
Csmd3	T	C	15: 47,559,257 (GRCm39)		probably null	Het
D430041D05Rik	GTGATGATGATGATGATGATG	GTGATGATGATGATGATG	2: 104,051,553 (GRCm39)		probably benign	Het
Dbil5	A	G	11: 76,109,308 (GRCm39)	M71V	probably benign	Het
Ddhd1	A	C	14: 45,833,004 (GRCm39)	L864R	probably damaging	Het
Dnah11	A	G	12: 118,024,052 (GRCm39)	C1900R	probably damaging	Het
Dnah2	A	G	11: 69,405,514 (GRCm39)	V666A	probably benign	Het
Dnah5	T	A	15: 28,252,569 (GRCm39)	M754K	probably benign	Het
Dnajc15	A	T	14: 78,063,854 (GRCm39)	S145T	probably benign	Het
Drap1	A	G	19: 5,474,285 (GRCm39)	F25S	probably damaging	Het
Fam83e	G	A	7: 45,376,135 (GRCm39)	E283K	probably damaging	Het
Fbxo48	G	T	11: 16,903,368 (GRCm39)		probably benign	Het
Fndc3a	A	T	14: 72,793,997 (GRCm39)	I892N	probably damaging	Het
Gcn1	A	G	5: 115,753,611 (GRCm39)	T2321A	probably benign	Het
Greb1l	A	G	18: 10,554,997 (GRCm39)	D1681G	possibly damaging	Het
Hmcn2	A	C	2: 31,294,899 (GRCm39)	T2563P	probably damaging	Het
Iqcd	A	T	5: 120,738,300 (GRCm39)	K39N	probably damaging	Het
Kank2	A	G	9: 21,685,871 (GRCm39)	S668P	probably damaging	Het
Kcng1	T	A	2: 168,110,961 (GRCm39)	N68Y	probably damaging	Het
Kmt5b	T	A	19: 3,836,633 (GRCm39)		probably null	Het
Lama2	T	A	10: 27,200,750 (GRCm39)	I533F	possibly damaging	Het
Lcmt1	T	A	7: 123,002,131 (GRCm39)	I132N	probably damaging	Het
Mcph1	T	C	8: 18,851,428 (GRCm39)	I807T	probably damaging	Het
Mdn1	A	G	4: 32,722,315 (GRCm39)	I2366V	probably benign	Het
Moxd1	T	C	10: 24,176,217 (GRCm39)	W558R	probably damaging	Het
Mtus2	A	C	5: 148,013,362 (GRCm39)	K52Q	probably benign	Het
Myrf	T	C	19: 10,202,851 (GRCm39)	D141G	probably damaging	Het
Naca	G	T	10: 127,876,267 (GRCm39)		probably benign	Het
Ncoa7	T	C	10: 30,570,097 (GRCm39)	I249M	probably damaging	Het
Obox5	T	C	7: 15,492,558 (GRCm39)	V171A	probably damaging	Het
Or13e8	A	T	4: 43,697,134 (GRCm39)	V13D	possibly damaging	Het
Or13p3	T	A	4: 118,566,751 (GRCm39)	I49N	probably damaging	Het
Or2c1	T	C	16: 3,657,521 (GRCm39)	I228T	probably damaging	Het
Or5ak20	A	T	2: 85,184,243 (GRCm39)	V9E	probably damaging	Het
Or6c219	A	G	10: 129,781,379 (GRCm39)	L69P	probably damaging	Het
Or7a36	C	A	10: 78,819,820 (GRCm39)	N32K	probably damaging	Het
Parp4	A	G	14: 56,839,752 (GRCm39)	T487A	probably damaging	Het
Pclo	A	G	5: 14,729,845 (GRCm39)		probably benign	Het
Pcnx2	G	A	8: 126,500,669 (GRCm39)	R1474C	probably damaging	Het
Pkd1l3	A	G	8: 110,341,445 (GRCm39)	I99M	unknown	Het
Ruvbl1	T	C	6: 88,456,136 (GRCm39)	V70A	probably damaging	Het
Sart1	G	A	19: 5,430,287 (GRCm39)	P788L	probably damaging	Het
Sdk1	A	G	5: 141,984,634 (GRCm39)	T740A	probably benign	Het
Serpinb1c	T	C	13: 33,072,979 (GRCm39)	D61G	possibly damaging	Het
Sfi1	TCGC	TC	11: 3,096,254 (GRCm39)		probably null	Het
Slc24a5	G	A	2: 124,922,782 (GRCm39)	G152S	probably damaging	Het
Slc6a4	A	T	11: 76,910,022 (GRCm39)	I426F	possibly damaging	Het
Srsf4	T	A	4: 131,625,006 (GRCm39)	D134E	probably damaging	Het
Stk33	C	T	7: 108,879,027 (GRCm39)	V441I	probably benign	Het
Sult1c2	A	G	17: 54,143,927 (GRCm39)		probably null	Het
Tdpoz4	T	A	3: 93,703,835 (GRCm39)	V44E	probably benign	Het
Tdrd6	G	A	17: 43,936,515 (GRCm39)	S1511L	probably damaging	Het
Tmprss13	C	A	9: 45,254,529 (GRCm39)	T432K	probably damaging	Het
Traf7	A	G	17: 24,729,527 (GRCm39)	L428P	probably damaging	Het
Tubb1	T	C	2: 174,299,215 (GRCm39)	I299T	probably benign	Het
Vmn1r158	A	T	7: 22,489,772 (GRCm39)	W146R	probably damaging	Het
Vmn1r42	A	G	6: 89,822,059 (GRCm39)	I170T	possibly damaging	Het
Vmn1r42	C	T	6: 89,822,363 (GRCm39)	G69S	probably damaging	Het
Vmn2r116	A	T	17: 23,606,063 (GRCm39)	H325L	probably damaging	Het
Zfp516	T	A	18: 83,011,300 (GRCm39)	L1111H	possibly damaging	Het
Zfp61	C	G	7: 23,990,635 (GRCm39)	K505N	probably damaging	Het
Zfp949	A	T	9: 88,451,830 (GRCm39)	K467*	probably null	Het

Other mutations in Zfp653

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02541:Zfp653	APN	9	21,967,079 (GRCm39)	missense	probably damaging	1.00
PIT4403001:Zfp653	UTSW	9	21,977,053 (GRCm39)	missense	probably damaging	0.96
R1245:Zfp653	UTSW	9	21,967,718 (GRCm39)	missense	probably damaging	1.00
R1473:Zfp653	UTSW	9	21,969,516 (GRCm39)	missense	possibly damaging	0.92
R1564:Zfp653	UTSW	9	21,967,155 (GRCm39)	missense	probably damaging	1.00
R1574:Zfp653	UTSW	9	21,969,274 (GRCm39)	nonsense	probably null
R2851:Zfp653	UTSW	9	21,968,862 (GRCm39)	missense	probably benign	0.09
R2852:Zfp653	UTSW	9	21,968,862 (GRCm39)	missense	probably benign	0.09
R2967:Zfp653	UTSW	9	21,977,026 (GRCm39)	missense	probably damaging	1.00
R4937:Zfp653	UTSW	9	21,967,074 (GRCm39)	missense	probably damaging	1.00
R5390:Zfp653	UTSW	9	21,969,099 (GRCm39)	critical splice donor site	probably null
R6135:Zfp653	UTSW	9	21,969,558 (GRCm39)	missense	probably damaging	0.97
R6798:Zfp653	UTSW	9	21,968,668 (GRCm39)	missense	probably damaging	1.00
R7146:Zfp653	UTSW	9	21,977,195 (GRCm39)	missense	probably damaging	1.00
R7258:Zfp653	UTSW	9	21,977,116 (GRCm39)	missense	probably benign	0.07
R7486:Zfp653	UTSW	9	21,967,824 (GRCm39)	missense	probably damaging	1.00
R7515:Zfp653	UTSW	9	21,982,427 (GRCm39)	missense	probably damaging	1.00
R8339:Zfp653	UTSW	9	21,969,213 (GRCm39)	missense	probably damaging	0.99
R9307:Zfp653	UTSW	9	21,969,321 (GRCm39)	missense	possibly damaging	0.94
R9491:Zfp653	UTSW	9	21,969,622 (GRCm39)	nonsense	probably null

Predicted Primers

Posted On

2017-12-01