Mutagenetix > Incidental Mutation

Incidental Mutation 'R5426:Med12l'

501021

Institutional Source

Beutler Lab

Gene Symbol

Med12l

Ensembl Gene

ENSMUSG00000056476

Gene Name

mediator complex subunit 12-like

Synonyms

MMRRC Submission

042992-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.308) question?

Stock #

R5426 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

58913246-59226103 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 59156143 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 1220 (M1220I)

Ref Sequence

ENSEMBL: ENSMUSP00000127038 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040325] [ENSMUST00000050360] [ENSMUST00000164225] [ENSMUST00000199609] [ENSMUST00000199659]

AlphaFold

Q8BQM9

Predicted Effect

probably benign
Transcript: ENSMUST00000040325
AA Change: M1185I

PolyPhen 2 Score 0.074 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000042269
Gene: ENSMUSG00000056476
AA Change: M1185I

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	730	2.6e-207	PFAM
low complexity region	744	758	N/A	INTRINSIC
low complexity region	853	872	N/A	INTRINSIC
low complexity region	1455	1466	N/A	INTRINSIC
low complexity region	1728	1742	N/A	INTRINSIC
low complexity region	1769	1783	N/A	INTRINSIC
Pfam:Med12-PQL	1803	2029	2.3e-14	PFAM
low complexity region	2055	2076	N/A	INTRINSIC
low complexity region	2083	2101	N/A	INTRINSIC
low complexity region	2116	2136	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000050360

SMART Domains

Protein: ENSMUSP00000051353
Gene: ENSMUSG00000036353

Domain	Start	End	E-Value	Type
Pfam:7tm_1	48	304	1.3e-40	PFAM
low complexity region	322	335	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000164225
AA Change: M1220I

PolyPhen 2 Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000127038
Gene: ENSMUSG00000056476
AA Change: M1220I

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	283	765	5e-187	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1763	1777	N/A	INTRINSIC
low complexity region	1804	1818	N/A	INTRINSIC
Pfam:Med12-PQL	1840	2063	9.7e-66	PFAM
low complexity region	2090	2111	N/A	INTRINSIC
low complexity region	2118	2136	N/A	INTRINSIC
low complexity region	2151	2171	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000199609

SMART Domains

Protein: ENSMUSP00000143521
Gene: ENSMUSG00000036353

Domain	Start	End	E-Value	Type
Pfam:7tm_1	23	304	1.5e-31	PFAM
low complexity region	322	335	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000199659
AA Change: M1220I

PolyPhen 2 Score 0.505 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000142903
Gene: ENSMUSG00000056476
AA Change: M1220I

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	765	5.5e-209	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1761	1775	N/A	INTRINSIC
low complexity region	1802	1816	N/A	INTRINSIC
Pfam:Med12-PQL	1836	2062	1.7e-15	PFAM
low complexity region	2088	2130	N/A	INTRINSIC
low complexity region	2144	2164	N/A	INTRINSIC

Meta Mutation Damage Score

0.1809

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.6%

Validation Efficiency

97% (85/88)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is part of the Mediator complex, which is involved in transcriptional coactivation of nearly all RNA polymerase II-dependent genes. The Mediator complex links gene-specific transcriptional activators with the basal transcription machinery. [provided by RefSeq, May 2010]

Allele List at MGI

All alleles(4) : Targeted(3) Gene trapped(1)

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010315B03Rik	T	A	9: 124,056,633 (GRCm39)	H122L	probably damaging	Het
9330182O14Rik	G	A	15: 40,011,932 (GRCm39)	M90I	unknown	Het
9330182O14Rik	G	A	15: 40,011,933 (GRCm39)	D91N	unknown	Het
Abca13	T	A	11: 9,240,722 (GRCm39)	S862T	probably damaging	Het
Acyp2	C	T	11: 30,456,354 (GRCm39)	E98K	possibly damaging	Het
Adgb	C	A	10: 10,226,004 (GRCm39)	A1405S	probably benign	Het
Adpgk	T	A	9: 59,204,832 (GRCm39)	V86E	probably damaging	Het
Agfg2	G	C	5: 137,666,020 (GRCm39)	P80A	probably damaging	Het
Akap11	G	A	14: 78,736,304 (GRCm39)	Q1829*	probably null	Het
Aldh1l1	G	A	6: 90,536,281 (GRCm39)	R62Q	probably benign	Het
Amd1	G	T	10: 40,166,183 (GRCm39)	D265E	probably damaging	Het
Aox4	A	T	1: 58,259,253 (GRCm39)	Q106L	probably damaging	Het
Arap2	A	T	5: 62,800,159 (GRCm39)	N1289K	probably benign	Het
Arhgef12	T	C	9: 42,897,880 (GRCm39)	S874G	probably damaging	Het
Ccdc166	T	C	15: 75,853,945 (GRCm39)	Q45R	possibly damaging	Het
Cdc45	C	T	16: 18,614,647 (GRCm39)	R205H	probably damaging	Het
Cdk14	A	G	5: 4,938,975 (GRCm39)	S388P	possibly damaging	Het
Chgb	A	T	2: 132,635,453 (GRCm39)	Q465L	possibly damaging	Het
Commd9	T	A	2: 101,729,220 (GRCm39)	W109R	probably damaging	Het
Cspg4b	G	A	13: 113,505,587 (GRCm39)	V2239I	probably benign	Het
Cyp2b9	T	C	7: 25,887,080 (GRCm39)	V163A	probably benign	Het
Dcpp2	T	A	17: 24,118,287 (GRCm39)	H27Q	possibly damaging	Het
Ddn	T	A	15: 98,704,347 (GRCm39)	E315V	possibly damaging	Het
Defb29	C	A	2: 152,380,868 (GRCm39)	C47F	probably damaging	Het
Dnah7b	T	C	1: 46,281,366 (GRCm39)	M2809T	possibly damaging	Het
Dock4	A	T	12: 40,795,744 (GRCm39)	I854F	probably damaging	Het
Dsg4	T	C	18: 20,591,541 (GRCm39)	Y427H	probably damaging	Het
E130114P18Rik	C	T	4: 97,578,907 (GRCm39)	A23T	unknown	Het
Flacc1	T	A	1: 58,706,045 (GRCm39)	K275*	probably null	Het
Gemin5	A	G	11: 58,016,113 (GRCm39)	S1297P	probably benign	Het
Gm10309	A	C	17: 86,806,161 (GRCm39)		probably benign	Het
Gpatch11	T	A	17: 79,148,663 (GRCm39)	S155R	possibly damaging	Het
Grin2b	C	A	6: 135,709,366 (GRCm39)	Q1393H	probably damaging	Het
Heatr5b	C	T	17: 79,081,142 (GRCm39)	C1370Y	probably damaging	Het
Hmcn2	C	T	2: 31,226,556 (GRCm39)	T177I	possibly damaging	Het
Ildr1	A	T	16: 36,529,981 (GRCm39)	I123F	probably damaging	Het
Kalrn	A	T	16: 34,083,023 (GRCm39)	V644D	probably damaging	Het
Kifap3	A	G	1: 163,607,440 (GRCm39)	M1V	probably null	Het
Klhl11	A	G	11: 100,354,942 (GRCm39)	L293P	probably damaging	Het
Klrc3	G	A	6: 129,618,513 (GRCm39)	S98L	probably benign	Het
Lrrc37	A	T	11: 103,511,586 (GRCm39)	H127Q	unknown	Het
Ms4a7	C	T	19: 11,303,166 (GRCm39)		probably null	Het
Mybpc2	A	G	7: 44,159,253 (GRCm39)	V599A	probably benign	Het
Naalad2	T	C	9: 18,258,815 (GRCm39)	N487D	probably benign	Het
Nat8f7	T	G	6: 85,684,805 (GRCm39)	S12R	probably benign	Het
Nefm	T	A	14: 68,357,515 (GRCm39)		probably benign	Het
Nlrp5	T	C	7: 23,117,626 (GRCm39)	V450A	probably damaging	Het
Nps	G	A	7: 134,870,376 (GRCm39)		probably null	Het
Or13c7d	A	G	4: 43,770,168 (GRCm39)	I281T	probably benign	Het
Or2ag16	C	A	7: 106,351,773 (GRCm39)	S274I	probably benign	Het
Or4a74	T	C	2: 89,440,083 (GRCm39)	Y121C	probably damaging	Het
Or4b13	T	A	2: 90,082,955 (GRCm39)	K126*	probably null	Het
Or5e1	T	C	7: 108,354,924 (GRCm39)	L287P	possibly damaging	Het
Or5h24	G	T	16: 58,918,665 (GRCm39)	P230Q	possibly damaging	Het
Or5p67	T	C	7: 107,922,017 (GRCm39)	N289D	probably damaging	Het
Oxct2a	C	A	4: 123,216,506 (GRCm39)	G292W	possibly damaging	Het
Pramel32	T	A	4: 88,547,647 (GRCm39)		probably benign	Het
Prr27	T	C	5: 87,998,744 (GRCm39)		probably benign	Het
Ptx3	T	C	3: 66,128,143 (GRCm39)	M68T	probably damaging	Het
Rufy1	A	G	11: 50,312,561 (GRCm39)	L131S	probably damaging	Het
Septin7	T	A	9: 25,197,986 (GRCm39)	I100N	possibly damaging	Het
Sgms2	T	C	3: 131,135,446 (GRCm39)	I143V	probably benign	Het
Slc25a34	A	G	4: 141,350,877 (GRCm39)	V44A	probably damaging	Het
Slc28a3	T	A	13: 58,710,968 (GRCm39)	D518V	probably damaging	Het
Slc6a7	C	T	18: 61,136,308 (GRCm39)		probably null	Het
Slco4a1	C	A	2: 180,113,028 (GRCm39)	A420D	possibly damaging	Het
Smap1	T	C	1: 23,888,471 (GRCm39)	T180A	probably benign	Het
Specc1l	T	C	10: 75,103,384 (GRCm39)	S920P	probably benign	Het
Tgtp2	A	G	11: 48,950,083 (GRCm39)	M163T	probably benign	Het
Tiam1	G	T	16: 89,662,280 (GRCm39)	Q613K	possibly damaging	Het
Vsig10l	T	A	7: 43,114,247 (GRCm39)	S190T	probably damaging	Het
Vwa3b	A	G	1: 37,154,752 (GRCm39)	Y512C	probably damaging	Het
Wdfy3	T	A	5: 102,067,312 (GRCm39)	N1207Y	probably damaging	Het
Wdr17	C	T	8: 55,134,434 (GRCm39)	G349R	probably damaging	Het
Wdr70	A	G	15: 7,951,586 (GRCm39)	L417S	possibly damaging	Het
Wdr81	A	C	11: 75,341,722 (GRCm39)	S1182A	possibly damaging	Het
Zfp458	A	T	13: 67,405,256 (GRCm39)	C391*	probably null	Het
Zfp638	A	G	6: 83,953,396 (GRCm39)	E1167G	probably damaging	Het
Zfp763	T	A	17: 33,238,569 (GRCm39)	D192V	probably benign	Het
Zfp853	T	G	5: 143,274,624 (GRCm39)	Q332P	unknown	Het

Other mutations in Med12l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00272:Med12l	APN	3	58,949,757 (GRCm39)	missense	probably damaging	0.98
IGL00561:Med12l	APN	3	59,135,245 (GRCm39)	missense	probably benign
IGL00974:Med12l	APN	3	58,990,435 (GRCm39)	missense	probably damaging	1.00
IGL01024:Med12l	APN	3	58,980,762 (GRCm39)	missense	probably damaging	1.00
IGL01094:Med12l	APN	3	59,001,076 (GRCm39)	missense	probably damaging	0.99
IGL01134:Med12l	APN	3	58,949,696 (GRCm39)	missense	possibly damaging	0.91
IGL01535:Med12l	APN	3	59,169,680 (GRCm39)	missense	probably damaging	1.00
IGL01653:Med12l	APN	3	59,169,314 (GRCm39)	missense	probably damaging	1.00
IGL01735:Med12l	APN	3	59,170,675 (GRCm39)	missense	probably damaging	1.00
IGL01972:Med12l	APN	3	59,169,314 (GRCm39)	missense	probably damaging	1.00
IGL02005:Med12l	APN	3	59,152,368 (GRCm39)	missense	probably damaging	1.00
IGL02098:Med12l	APN	3	59,183,276 (GRCm39)	missense	possibly damaging	0.92
IGL02115:Med12l	APN	3	58,975,740 (GRCm39)	missense	probably benign	0.00
IGL02231:Med12l	APN	3	59,153,303 (GRCm39)	missense	probably damaging	1.00
IGL02259:Med12l	APN	3	59,153,264 (GRCm39)	missense	probably damaging	1.00
IGL02369:Med12l	APN	3	59,164,794 (GRCm39)	missense	probably benign	0.00
IGL02424:Med12l	APN	3	59,000,143 (GRCm39)	missense	probably benign	0.21
IGL02501:Med12l	APN	3	59,169,397 (GRCm39)	missense	possibly damaging	0.71
IGL02525:Med12l	APN	3	58,975,789 (GRCm39)	missense	probably benign	0.01
IGL02530:Med12l	APN	3	58,984,510 (GRCm39)	missense	probably damaging	1.00
IGL02735:Med12l	APN	3	59,001,067 (GRCm39)	missense	probably damaging	1.00
IGL02865:Med12l	APN	3	59,201,713 (GRCm39)	missense	probably damaging	1.00
IGL03183:Med12l	APN	3	58,944,976 (GRCm39)	splice site	probably null
IGL03264:Med12l	APN	3	59,208,788 (GRCm39)	nonsense	probably null
FR4304:Med12l	UTSW	3	59,183,403 (GRCm39)	small insertion	probably benign
FR4340:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
FR4342:Med12l	UTSW	3	59,183,415 (GRCm39)	small insertion	probably benign
FR4342:Med12l	UTSW	3	59,183,409 (GRCm39)	small insertion	probably benign
FR4449:Med12l	UTSW	3	59,183,384 (GRCm39)	nonsense	probably null
FR4548:Med12l	UTSW	3	59,183,403 (GRCm39)	small insertion	probably benign
FR4589:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
FR4976:Med12l	UTSW	3	59,183,398 (GRCm39)	small insertion	probably benign
P0007:Med12l	UTSW	3	58,998,816 (GRCm39)	splice site	probably benign
P0045:Med12l	UTSW	3	58,998,956 (GRCm39)	missense	probably damaging	0.99
R0030:Med12l	UTSW	3	59,156,076 (GRCm39)	missense	probably damaging	1.00
R0030:Med12l	UTSW	3	59,156,076 (GRCm39)	missense	probably damaging	1.00
R0148:Med12l	UTSW	3	58,945,075 (GRCm39)	missense	probably damaging	1.00
R0325:Med12l	UTSW	3	58,984,480 (GRCm39)	missense	possibly damaging	0.88
R0330:Med12l	UTSW	3	59,135,123 (GRCm39)	missense	probably damaging	1.00
R0388:Med12l	UTSW	3	59,000,925 (GRCm39)	splice site	probably benign
R0542:Med12l	UTSW	3	58,949,822 (GRCm39)	missense	probably damaging	1.00
R0624:Med12l	UTSW	3	58,945,123 (GRCm39)	nonsense	probably null
R0625:Med12l	UTSW	3	59,154,858 (GRCm39)	missense	probably damaging	1.00
R0671:Med12l	UTSW	3	59,172,350 (GRCm39)	missense	probably damaging	1.00
R0706:Med12l	UTSW	3	59,169,401 (GRCm39)	missense	probably damaging	1.00
R0785:Med12l	UTSW	3	59,168,253 (GRCm39)	missense	probably damaging	1.00
R1054:Med12l	UTSW	3	59,156,072 (GRCm39)	missense	probably damaging	0.99
R1102:Med12l	UTSW	3	59,152,257 (GRCm39)	missense	probably damaging	0.99
R1391:Med12l	UTSW	3	58,945,159 (GRCm39)	missense	probably benign	0.00
R1501:Med12l	UTSW	3	59,168,256 (GRCm39)	critical splice donor site	probably null
R1544:Med12l	UTSW	3	59,172,661 (GRCm39)	missense	possibly damaging	0.71
R1662:Med12l	UTSW	3	59,001,038 (GRCm39)	missense	probably damaging	1.00
R1670:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
R1839:Med12l	UTSW	3	58,975,740 (GRCm39)	missense	probably benign
R1854:Med12l	UTSW	3	59,168,193 (GRCm39)	missense	probably damaging	1.00
R2045:Med12l	UTSW	3	59,169,731 (GRCm39)	nonsense	probably null
R2070:Med12l	UTSW	3	59,152,326 (GRCm39)	missense	probably damaging	1.00
R2132:Med12l	UTSW	3	59,172,703 (GRCm39)	splice site	probably null
R2290:Med12l	UTSW	3	59,152,359 (GRCm39)	missense	probably damaging	1.00
R2325:Med12l	UTSW	3	59,139,875 (GRCm39)	missense	probably damaging	0.99
R2352:Med12l	UTSW	3	59,148,113 (GRCm39)	missense	probably damaging	1.00
R2484:Med12l	UTSW	3	59,205,259 (GRCm39)	missense	probably benign	0.18
R2906:Med12l	UTSW	3	59,164,503 (GRCm39)	missense	probably damaging	1.00
R3735:Med12l	UTSW	3	58,998,916 (GRCm39)	missense	probably damaging	1.00
R3736:Med12l	UTSW	3	58,998,916 (GRCm39)	missense	probably damaging	1.00
R3774:Med12l	UTSW	3	59,155,363 (GRCm39)	missense	probably damaging	0.97
R3957:Med12l	UTSW	3	58,980,589 (GRCm39)	missense	probably damaging	0.99
R4020:Med12l	UTSW	3	59,155,363 (GRCm39)	missense	probably damaging	0.97
R4087:Med12l	UTSW	3	59,205,342 (GRCm39)	missense	probably benign	0.00
R4231:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4233:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4235:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4236:Med12l	UTSW	3	59,164,644 (GRCm39)	splice site	probably null
R4327:Med12l	UTSW	3	59,172,688 (GRCm39)	missense	probably benign	0.01
R4328:Med12l	UTSW	3	59,172,688 (GRCm39)	missense	probably benign	0.01
R4346:Med12l	UTSW	3	58,938,976 (GRCm39)	missense	probably damaging	1.00
R4543:Med12l	UTSW	3	58,998,929 (GRCm39)	missense	probably damaging	1.00
R4559:Med12l	UTSW	3	58,914,523 (GRCm39)	critical splice donor site	probably null
R4776:Med12l	UTSW	3	59,140,633 (GRCm39)	missense	probably damaging	1.00
R4877:Med12l	UTSW	3	59,152,214 (GRCm39)	missense	probably damaging	1.00
R4983:Med12l	UTSW	3	59,169,350 (GRCm39)	missense	probably damaging	1.00
R5114:Med12l	UTSW	3	59,167,109 (GRCm39)	missense	possibly damaging	0.85
R5125:Med12l	UTSW	3	59,174,635 (GRCm39)	missense	possibly damaging	0.83
R5230:Med12l	UTSW	3	59,153,209 (GRCm39)	missense	probably damaging	1.00
R5407:Med12l	UTSW	3	59,165,622 (GRCm39)	missense	probably damaging	1.00
R5439:Med12l	UTSW	3	59,170,634 (GRCm39)	missense	probably null	1.00
R5449:Med12l	UTSW	3	59,167,127 (GRCm39)	missense	probably damaging	1.00
R5596:Med12l	UTSW	3	59,159,771 (GRCm39)	missense	probably benign	0.45
R5716:Med12l	UTSW	3	59,208,798 (GRCm39)	critical splice donor site	probably null
R5833:Med12l	UTSW	3	59,172,647 (GRCm39)	missense	possibly damaging	0.95
R5883:Med12l	UTSW	3	58,998,889 (GRCm39)	missense	probably damaging	1.00
R6264:Med12l	UTSW	3	59,163,423 (GRCm39)	missense	probably damaging	1.00
R6269:Med12l	UTSW	3	59,135,243 (GRCm39)	missense	probably damaging	1.00
R6394:Med12l	UTSW	3	59,142,508 (GRCm39)	missense	probably damaging	1.00
R6400:Med12l	UTSW	3	59,155,332 (GRCm39)	missense	probably damaging	1.00
R6475:Med12l	UTSW	3	59,164,500 (GRCm39)	missense	probably damaging	1.00
R6489:Med12l	UTSW	3	59,164,828 (GRCm39)	missense	probably damaging	0.99
R6654:Med12l	UTSW	3	59,169,713 (GRCm39)	missense	probably damaging	1.00
R6881:Med12l	UTSW	3	59,174,586 (GRCm39)	missense	probably benign	0.00
R7110:Med12l	UTSW	3	59,169,645 (GRCm39)	missense	possibly damaging	0.92
R7134:Med12l	UTSW	3	59,001,180 (GRCm39)	nonsense	probably null
R7137:Med12l	UTSW	3	59,165,675 (GRCm39)	missense	probably damaging	1.00
R7159:Med12l	UTSW	3	59,183,438 (GRCm39)	missense	probably benign
R7341:Med12l	UTSW	3	58,949,824 (GRCm39)	missense	possibly damaging	0.53
R7349:Med12l	UTSW	3	59,165,746 (GRCm39)	missense	probably damaging	1.00
R7413:Med12l	UTSW	3	58,998,971 (GRCm39)	missense	probably benign	0.00
R7495:Med12l	UTSW	3	59,152,194 (GRCm39)	missense	probably damaging	1.00
R7678:Med12l	UTSW	3	58,984,141 (GRCm39)	missense	probably damaging	1.00
R7697:Med12l	UTSW	3	59,148,078 (GRCm39)	missense	probably damaging	1.00
R7714:Med12l	UTSW	3	59,001,007 (GRCm39)	missense	probably benign	0.17
R7725:Med12l	UTSW	3	59,163,413 (GRCm39)	missense	probably damaging	1.00
R7846:Med12l	UTSW	3	59,172,355 (GRCm39)	missense	probably damaging	1.00
R7852:Med12l	UTSW	3	59,155,332 (GRCm39)	missense	probably damaging	1.00
R8080:Med12l	UTSW	3	59,172,607 (GRCm39)	missense	probably damaging	1.00
R8181:Med12l	UTSW	3	59,169,389 (GRCm39)	missense	probably damaging	1.00
R8223:Med12l	UTSW	3	58,993,784 (GRCm39)	missense	possibly damaging	0.79
R8560:Med12l	UTSW	3	58,945,026 (GRCm39)	missense	probably damaging	1.00
R8708:Med12l	UTSW	3	59,159,751 (GRCm39)	missense	probably benign	0.00
R8865:Med12l	UTSW	3	58,979,303 (GRCm39)	missense	probably benign
R8947:Med12l	UTSW	3	58,984,443 (GRCm39)	splice site	probably benign
R8976:Med12l	UTSW	3	59,183,329 (GRCm39)	missense	probably damaging	0.99
R9016:Med12l	UTSW	3	59,163,294 (GRCm39)	missense	probably damaging	0.96
R9183:Med12l	UTSW	3	58,984,498 (GRCm39)	missense	probably damaging	1.00
R9487:Med12l	UTSW	3	59,155,353 (GRCm39)	missense	probably benign
R9526:Med12l	UTSW	3	58,984,207 (GRCm39)	missense	probably damaging	0.96
R9802:Med12l	UTSW	3	59,169,346 (GRCm39)	missense	probably damaging	1.00
RF004:Med12l	UTSW	3	59,183,390 (GRCm39)	small insertion	probably benign
RF011:Med12l	UTSW	3	59,183,401 (GRCm39)	small insertion	probably benign
RF013:Med12l	UTSW	3	59,183,387 (GRCm39)	small insertion	probably benign
RF020:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
RF021:Med12l	UTSW	3	58,980,711 (GRCm39)	missense	probably benign	0.19
RF027:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF027:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF030:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
RF032:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,408 (GRCm39)	small insertion	probably benign
RF033:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF037:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
RF040:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF040:Med12l	UTSW	3	59,183,410 (GRCm39)	small insertion	probably benign
RF041:Med12l	UTSW	3	59,183,406 (GRCm39)	small insertion	probably benign
RF041:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,402 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,388 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,377 (GRCm39)	small insertion	probably benign
RF042:Med12l	UTSW	3	59,183,416 (GRCm39)	small insertion	probably benign
RF049:Med12l	UTSW	3	59,183,390 (GRCm39)	small insertion	probably benign
RF050:Med12l	UTSW	3	59,183,394 (GRCm39)	small insertion	probably benign
RF053:Med12l	UTSW	3	59,183,414 (GRCm39)	small insertion	probably benign
RF055:Med12l	UTSW	3	59,183,404 (GRCm39)	small insertion	probably benign
RF056:Med12l	UTSW	3	59,183,414 (GRCm39)	small insertion	probably benign
RF057:Med12l	UTSW	3	59,183,401 (GRCm39)	small insertion	probably benign
RF063:Med12l	UTSW	3	59,183,394 (GRCm39)	small insertion	probably benign
RF063:Med12l	UTSW	3	59,183,379 (GRCm39)	small insertion	probably benign
X0062:Med12l	UTSW	3	59,140,600 (GRCm39)	missense	probably damaging	1.00
Z1176:Med12l	UTSW	3	59,203,538 (GRCm39)	missense	probably benign	0.00
Z1176:Med12l	UTSW	3	59,152,364 (GRCm39)	missense	probably damaging	1.00
Z1176:Med12l	UTSW	3	58,998,838 (GRCm39)	missense	probably damaging	0.98
Z1177:Med12l	UTSW	3	59,155,296 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATTGCAGAAGGTGGTTAATGGC -3'
(R):5'- CCATTACTTGGGCTTAGGGAAAG -3'

Sequencing Primer
(F):5'- TTAATGGCAATGAAGAGGCCACTG -3'
(R):5'- AGTGGATGCTCACAGTCATC -3'

Posted On

2017-12-01