Mutagenetix > Incidental Mutation

Incidental Mutation 'R5656:Naa35'

501232

Institutional Source

Beutler Lab

Gene Symbol

Naa35

Ensembl Gene

ENSMUSG00000021555

Gene Name

N(alpha)-acetyltransferase 35, NatC auxiliary subunit

Synonyms

Mak10, A330027C19Rik, C030004C14Rik, A330021G12Rik

MMRRC Submission

043302-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.962) question?

Stock #

R5656 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

59733147-59782612 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

G to A at 59770680 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000127270 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022038] [ENSMUST00000164011] [ENSMUST00000165253] [ENSMUST00000172419]

AlphaFold

Q6PHQ8

Predicted Effect

silent
Transcript: ENSMUST00000022038

SMART Domains

Protein: ENSMUSP00000022038
Gene: ENSMUSG00000021555

Domain	Start	End	E-Value	Type
Pfam:Mak10	45	145	2.2e-29	PFAM
Pfam:Mak10	141	194	3.7e-10	PFAM
low complexity region	561	568	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000163395

Predicted Effect

probably benign
Transcript: ENSMUST00000164011

SMART Domains

Protein: ENSMUSP00000127270
Gene: ENSMUSG00000021555

Domain	Start	End	E-Value	Type
Pfam:Mak10	1	119	1.9e-37	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000165129

Predicted Effect

silent
Transcript: ENSMUST00000165253

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000165841

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000168242

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000168633

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000171391

Predicted Effect

silent
Transcript: ENSMUST00000172166

Predicted Effect

silent
Transcript: ENSMUST00000172419

SMART Domains

Protein: ENSMUSP00000126079
Gene: ENSMUSG00000021555

Domain	Start	End	E-Value	Type
Pfam:Mak10	40	193	1.2e-55	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000224194

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933405L10Rik	A	G	8: 106,436,144 (GRCm39)	S139G	probably benign	Het
Adrb3	T	C	8: 27,717,405 (GRCm39)	D348G	probably damaging	Het
Atg2b	A	G	12: 105,587,587 (GRCm39)	V1959A	probably benign	Het
Bicral	G	A	17: 47,119,295 (GRCm39)	T742M	probably damaging	Het
Bub1b	T	A	2: 118,435,912 (GRCm39)	I60N	probably damaging	Het
Ccdc162	A	G	10: 41,445,930 (GRCm39)	V414A	probably benign	Het
Cd22	T	G	7: 30,569,198 (GRCm39)	Y612S	probably damaging	Het
Cd68	T	A	11: 69,555,247 (GRCm39)	I320F	probably damaging	Het
Clca3a2	A	T	3: 144,503,393 (GRCm39)	N852K	probably benign	Het
Cpa6	T	A	1: 10,399,739 (GRCm39)	H363L	probably benign	Het
Ddx18	A	T	1: 121,489,087 (GRCm39)	L320Q	probably damaging	Het
Dnah5	A	G	15: 28,421,210 (GRCm39)	D3849G	probably benign	Het
Eci1	T	A	17: 24,656,283 (GRCm39)	N164K	probably damaging	Het
Efs	T	C	14: 55,154,584 (GRCm39)	T552A	probably damaging	Het
Fbp1	C	A	13: 63,023,010 (GRCm39)	V96L	probably damaging	Het
Gtf3c1	T	A	7: 125,261,826 (GRCm39)	N1139I	probably benign	Het
Gucy1b2	T	C	14: 62,660,430 (GRCm39)	Y152C	probably damaging	Het
Gxylt1	A	T	15: 93,143,542 (GRCm39)	L362Q	probably damaging	Het
Iqcd	A	G	5: 120,743,191 (GRCm39)		probably null	Het
Klhl41	T	A	2: 69,513,876 (GRCm39)	I585N	possibly damaging	Het
Map6	A	G	7: 98,985,505 (GRCm39)	K470E	probably damaging	Het
Mast3	T	C	8: 71,238,865 (GRCm39)	T496A	probably damaging	Het
Mbd6	A	T	10: 127,121,155 (GRCm39)		probably benign	Het
Melk	A	G	4: 44,312,237 (GRCm39)	K183R	possibly damaging	Het
Mta1	T	C	12: 113,086,759 (GRCm39)	V152A	probably damaging	Het
Nav3	A	C	10: 109,600,494 (GRCm39)	S1378A	probably damaging	Het
Ncapd3	T	A	9: 26,962,941 (GRCm39)	D415E	possibly damaging	Het
Nlrp4f	G	A	13: 65,338,685 (GRCm39)	R651*	probably null	Het
Or1e1f	T	A	11: 73,855,536 (GRCm39)	M34K	probably damaging	Het
Or5b121	A	G	19: 13,507,744 (GRCm39)	T280A	probably benign	Het
Or5p72	A	G	7: 108,021,825 (GRCm39)	I16V	probably benign	Het
P2rx7	A	T	5: 122,811,780 (GRCm39)	R364W	probably damaging	Het
Phactr2	T	C	10: 13,264,447 (GRCm39)	D2G	probably benign	Het
Phc3	G	T	3: 31,020,015 (GRCm39)	S28R	probably damaging	Het
Ppfia1	A	T	7: 144,073,711 (GRCm39)		probably null	Het
Prdm10	C	T	9: 31,264,713 (GRCm39)	T667M	probably benign	Het
Pwwp2b	T	A	7: 138,835,887 (GRCm39)	S443T	possibly damaging	Het
Pzp	T	C	6: 128,467,035 (GRCm39)	T1113A	probably damaging	Het
Rapgef6	A	G	11: 54,526,962 (GRCm39)	E551G	possibly damaging	Het
Sec23ip	A	G	7: 128,378,508 (GRCm39)	Y774C	probably damaging	Het
Setdb2	T	C	14: 59,656,567 (GRCm39)	D266G	probably damaging	Het
Shank1	T	C	7: 44,002,310 (GRCm39)	V1343A	probably benign	Het
Slf2	T	A	19: 44,961,674 (GRCm39)	D1064E	probably benign	Het
Slu7	A	G	11: 43,334,245 (GRCm39)	K424E	probably benign	Het
Smg1	A	T	7: 117,753,887 (GRCm39)		probably benign	Het
Sptlc2	A	T	12: 87,393,535 (GRCm39)	L264Q	probably damaging	Het
Sra1	A	G	18: 36,811,460 (GRCm39)	S93P	probably damaging	Het
Sult1c2	T	C	17: 54,271,680 (GRCm39)	E169G	probably benign	Het
Sv2a	A	G	3: 96,092,888 (GRCm39)	D196G	probably damaging	Het
Tbc1d22b	A	G	17: 29,813,754 (GRCm39)	I362M	probably damaging	Het
Tenm3	T	C	8: 48,681,797 (GRCm39)	D2611G	probably damaging	Het
Tmem43	T	C	6: 91,457,690 (GRCm39)	F191L	probably benign	Het
Trbv13-2	T	A	6: 41,098,628 (GRCm39)	Y68N	probably benign	Het
Ttn	T	G	2: 76,604,998 (GRCm39)	D18312A	possibly damaging	Het
Ublcp1	A	G	11: 44,356,433 (GRCm39)	V95A	probably damaging	Het
Usp17ld	A	G	7: 102,900,047 (GRCm39)	V295A	probably damaging	Het
Vmn1r29	T	A	6: 58,285,152 (GRCm39)	L291M	possibly damaging	Het
Vsig10l	C	T	7: 43,113,575 (GRCm39)	R176*	probably null	Het
Zbtb46	A	G	2: 181,065,210 (GRCm39)		probably null	Het
Zfp644	A	G	5: 106,785,848 (GRCm39)	V233A	probably benign	Het

Other mutations in Naa35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00476:Naa35	APN	13	59,777,869 (GRCm39)	missense	probably damaging	1.00
IGL00743:Naa35	APN	13	59,778,485 (GRCm39)	missense	probably benign	0.33
IGL01335:Naa35	APN	13	59,764,610 (GRCm39)	missense	probably damaging	1.00
IGL01385:Naa35	APN	13	59,748,880 (GRCm39)	missense	probably damaging	1.00
IGL01541:Naa35	APN	13	59,748,777 (GRCm39)	missense	probably damaging	1.00
IGL02129:Naa35	APN	13	59,757,339 (GRCm39)	missense	probably damaging	0.99
IGL02867:Naa35	APN	13	59,756,668 (GRCm39)	intron	probably benign
IGL02966:Naa35	APN	13	59,734,085 (GRCm39)	missense	probably benign
IGL03260:Naa35	APN	13	59,775,699 (GRCm39)	missense	probably benign	0.05
R0312:Naa35	UTSW	13	59,757,395 (GRCm39)	missense	probably benign	0.01
R0557:Naa35	UTSW	13	59,775,778 (GRCm39)	missense	probably damaging	0.99
R1553:Naa35	UTSW	13	59,766,093 (GRCm39)	critical splice donor site	probably null
R1611:Naa35	UTSW	13	59,776,747 (GRCm39)	missense	probably benign	0.01
R1676:Naa35	UTSW	13	59,760,490 (GRCm39)	missense	probably damaging	1.00
R3709:Naa35	UTSW	13	59,765,846 (GRCm39)	splice site	probably benign
R3896:Naa35	UTSW	13	59,755,109 (GRCm39)	missense	probably damaging	1.00
R5001:Naa35	UTSW	13	59,773,345 (GRCm39)	missense	possibly damaging	0.75
R5647:Naa35	UTSW	13	59,770,680 (GRCm39)	unclassified	probably benign
R5649:Naa35	UTSW	13	59,770,680 (GRCm39)	unclassified	probably benign
R5650:Naa35	UTSW	13	59,770,680 (GRCm39)	unclassified	probably benign
R6734:Naa35	UTSW	13	59,756,005 (GRCm39)	missense	possibly damaging	0.83
R6735:Naa35	UTSW	13	59,773,378 (GRCm39)	missense	probably damaging	1.00
R6985:Naa35	UTSW	13	59,775,757 (GRCm39)	missense	probably benign	0.03
R7165:Naa35	UTSW	13	59,733,997 (GRCm39)	missense	probably benign	0.05
R7531:Naa35	UTSW	13	59,765,755 (GRCm39)	nonsense	probably null
R7713:Naa35	UTSW	13	59,745,919 (GRCm39)	missense	probably benign	0.01
R7739:Naa35	UTSW	13	59,747,598 (GRCm39)	missense	probably damaging	1.00
R8685:Naa35	UTSW	13	59,734,036 (GRCm39)	missense	probably benign	0.43
R8818:Naa35	UTSW	13	59,748,761 (GRCm39)	missense	probably damaging	1.00
R8838:Naa35	UTSW	13	59,775,775 (GRCm39)	missense	probably benign
R9006:Naa35	UTSW	13	59,748,842 (GRCm39)	missense	possibly damaging	0.68
R9132:Naa35	UTSW	13	59,772,341 (GRCm39)	missense	possibly damaging	0.84
R9403:Naa35	UTSW	13	59,748,817 (GRCm39)	missense	possibly damaging	0.74

Predicted Primers

PCR Primer
(F):5'- TGGACACCTTACTCTGACTACAC -3'
(R):5'- CTCACTCAAGCAGTAAGAGGAG -3'

Sequencing Primer
(F):5'- GACACCTTACTCTGACTACACTTGTG -3'
(R):5'- CTGTGCAGCATAATTTTCCA -3'

Posted On

2017-12-01