Incidental Mutation 'R6160:Ints4'
ID 501891
Institutional Source Beutler Lab
Gene Symbol Ints4
Ensembl Gene ENSMUSG00000025133
Gene Name integrator complex subunit 4
Synonyms 2610034N24Rik
MMRRC Submission 044307-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.961) question?
Stock # R6160 (G1)
Quality Score 225.009
Status Validated
Chromosome 7
Chromosomal Location 97130163-97190602 bp(+) (GRCm39)
Type of Mutation splice site
DNA Base Change (assembly) T to A at 97158790 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000026126 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000026126] [ENSMUST00000026126] [ENSMUST00000026126] [ENSMUST00000026126]
AlphaFold Q8CIM8
Predicted Effect probably null
Transcript: ENSMUST00000026126
SMART Domains Protein: ENSMUSP00000026126
Gene: ENSMUSG00000025133

DomainStartEndE-ValueType
Pfam:HEAT_2 153 258 9.8e-9 PFAM
Pfam:Cohesin_HEAT 179 219 7.7e-6 PFAM
low complexity region 518 527 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000026126
SMART Domains Protein: ENSMUSP00000026126
Gene: ENSMUSG00000025133

DomainStartEndE-ValueType
Pfam:HEAT_2 153 258 9.8e-9 PFAM
Pfam:Cohesin_HEAT 179 219 7.7e-6 PFAM
low complexity region 518 527 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000026126
SMART Domains Protein: ENSMUSP00000026126
Gene: ENSMUSG00000025133

DomainStartEndE-ValueType
Pfam:HEAT_2 153 258 9.8e-9 PFAM
Pfam:Cohesin_HEAT 179 219 7.7e-6 PFAM
low complexity region 518 527 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000026126
SMART Domains Protein: ENSMUSP00000026126
Gene: ENSMUSG00000025133

DomainStartEndE-ValueType
Pfam:HEAT_2 153 258 9.8e-9 PFAM
Pfam:Cohesin_HEAT 179 219 7.7e-6 PFAM
low complexity region 518 527 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000205876
Predicted Effect noncoding transcript
Transcript: ENSMUST00000206644
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.2%
  • 20x: 94.8%
Validation Efficiency 94% (62/66)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] INTS4 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]
Allele List at MGI
Other mutations in this stock
Total: 66 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930433I11Rik T A 7: 40,642,950 (GRCm39) S297R possibly damaging Het
5530400C23Rik A G 6: 133,271,289 (GRCm39) E111G possibly damaging Het
8030423J24Rik T A 13: 71,032,029 (GRCm39) S34T unknown Het
Ace3 T A 11: 105,885,558 (GRCm39) W20R possibly damaging Het
Adgrf1 A G 17: 43,621,578 (GRCm39) E605G probably damaging Het
Arhgap44 G A 11: 65,053,375 (GRCm39) probably benign Het
Atm A C 9: 53,402,259 (GRCm39) H1404Q probably benign Het
AW209491 A G 13: 14,811,306 (GRCm39) E53G probably damaging Het
Bhmt1b G A 18: 87,775,245 (GRCm39) C256Y probably damaging Het
Cdt1 C T 8: 123,298,107 (GRCm39) T366I probably benign Het
Cerk A T 15: 86,026,974 (GRCm39) C179S probably benign Het
Cldn10 T C 14: 119,099,255 (GRCm39) V123A possibly damaging Het
Clip1 T C 5: 123,751,604 (GRCm39) K726E possibly damaging Het
Dcaf12 A T 4: 41,294,043 (GRCm39) Y365N probably damaging Het
Dennd6b A G 15: 89,073,024 (GRCm39) L171P probably damaging Het
Dip2c G A 13: 9,583,290 (GRCm39) V91I probably benign Het
Dlec1 A G 9: 118,972,387 (GRCm39) I1431V probably benign Het
Egln1 T C 8: 125,675,231 (GRCm39) D188G probably damaging Het
Enpp5 T A 17: 44,392,259 (GRCm39) N229K possibly damaging Het
Fmo1 T G 1: 162,663,867 (GRCm39) I221L probably benign Het
Fsip2 G A 2: 82,818,289 (GRCm39) W4674* probably null Het
Gm12185 G A 11: 48,799,255 (GRCm39) Q413* probably null Het
Gm7298 A T 6: 121,741,886 (GRCm39) H436L probably benign Het
Gucy2c G A 6: 136,717,684 (GRCm39) Q430* probably null Het
Hgd A G 16: 37,433,660 (GRCm39) H134R probably damaging Het
Hoxd8 A G 2: 74,536,343 (GRCm39) E151G probably damaging Het
Il15ra A G 2: 11,724,827 (GRCm39) D99G probably damaging Het
Itgb1 T A 8: 129,446,764 (GRCm39) F426L possibly damaging Het
Itpr1 A C 6: 108,495,716 (GRCm39) I2534L probably benign Het
Kcnq4 T A 4: 120,573,756 (GRCm39) H235L probably damaging Het
Kcnt1 T A 2: 25,782,395 (GRCm39) I178N probably damaging Het
Kidins220 A G 12: 25,047,310 (GRCm39) D252G probably damaging Het
Krt23 A G 11: 99,376,544 (GRCm39) I204T probably damaging Het
Lipo4 A G 19: 33,480,693 (GRCm39) L225P probably damaging Het
Lrp3 T C 7: 34,903,548 (GRCm39) D245G possibly damaging Het
Mmp16 A G 4: 18,051,857 (GRCm39) D282G probably damaging Het
Myo1c A T 11: 75,541,568 (GRCm39) H18L probably benign Het
Myo1f C A 17: 33,823,318 (GRCm39) P981Q probably benign Het
Nle1 A T 11: 82,798,983 (GRCm39) F33I probably benign Het
Nlrp4e T G 7: 23,020,731 (GRCm39) M406R probably damaging Het
Obscn A T 11: 58,942,611 (GRCm39) V4857E probably damaging Het
Palb2 A T 7: 121,727,643 (GRCm39) probably null Het
Phospho1 A T 11: 95,721,450 (GRCm39) E22V probably damaging Het
Pom121l2 C T 13: 22,167,838 (GRCm39) S703L possibly damaging Het
Prex2 T C 1: 11,064,075 (GRCm39) L20P probably damaging Het
Psmb7 T C 2: 38,533,393 (GRCm39) T45A probably damaging Het
R3hdm2 T C 10: 127,320,376 (GRCm39) I532T probably damaging Het
Rcn1 T C 2: 105,222,362 (GRCm39) D208G probably damaging Het
Recql5 A G 11: 115,823,613 (GRCm39) probably null Het
Resf1 G A 6: 149,233,005 (GRCm39) probably null Het
Rfc4 A T 16: 22,933,433 (GRCm39) I242N probably damaging Het
Rims1 T C 1: 22,503,235 (GRCm39) Y650C probably damaging Het
Shc2 C T 10: 79,462,853 (GRCm39) probably null Het
Slc14a2 A T 18: 78,202,190 (GRCm39) probably null Het
Slc6a6 A T 6: 91,716,995 (GRCm39) probably null Het
Synj2 C A 17: 6,058,336 (GRCm39) H275N possibly damaging Het
T A T 17: 8,660,618 (GRCm39) T410S probably benign Het
Tars3 A G 7: 65,332,527 (GRCm39) I543V probably benign Het
Tbc1d8 T A 1: 39,411,484 (GRCm39) K1117N probably damaging Het
Tm7sf3 A T 6: 146,507,787 (GRCm39) L425* probably null Het
Trav14-3 A G 14: 54,000,978 (GRCm39) Y63C probably damaging Het
Tyro3 A C 2: 119,633,751 (GRCm39) D133A probably damaging Het
Vmn1r119 T G 7: 20,745,740 (GRCm39) H214P possibly damaging Het
Vmn2r120 G A 17: 57,816,418 (GRCm39) P646S probably benign Het
Zbtb7c A T 18: 76,278,904 (GRCm39) Y454F probably benign Het
Zmym2 T C 14: 57,187,766 (GRCm39) L1144P probably damaging Het
Other mutations in Ints4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00765:Ints4 APN 7 97,184,412 (GRCm39) missense probably damaging 1.00
IGL01736:Ints4 APN 7 97,175,849 (GRCm39) missense probably benign 0.01
IGL01862:Ints4 APN 7 97,190,360 (GRCm39) missense probably damaging 1.00
IGL02277:Ints4 APN 7 97,136,665 (GRCm39) missense probably damaging 1.00
IGL02396:Ints4 APN 7 97,187,107 (GRCm39) missense possibly damaging 0.64
IGL02661:Ints4 APN 7 97,144,912 (GRCm39) missense probably benign
IGL02750:Ints4 APN 7 97,166,964 (GRCm39) critical splice donor site probably null
IGL03108:Ints4 APN 7 97,140,137 (GRCm39) critical splice acceptor site probably null
R0332:Ints4 UTSW 7 97,166,925 (GRCm39) missense probably damaging 1.00
R0449:Ints4 UTSW 7 97,178,430 (GRCm39) missense probably damaging 0.97
R1065:Ints4 UTSW 7 97,157,099 (GRCm39) critical splice donor site probably null
R1722:Ints4 UTSW 7 97,162,786 (GRCm39) missense probably benign 0.24
R2060:Ints4 UTSW 7 97,150,970 (GRCm39) missense possibly damaging 0.93
R2211:Ints4 UTSW 7 97,158,957 (GRCm39) missense possibly damaging 0.63
R3731:Ints4 UTSW 7 97,155,308 (GRCm39) missense probably benign 0.18
R3881:Ints4 UTSW 7 97,165,464 (GRCm39) missense possibly damaging 0.94
R4089:Ints4 UTSW 7 97,178,462 (GRCm39) nonsense probably null
R4192:Ints4 UTSW 7 97,156,940 (GRCm39) missense probably damaging 1.00
R4479:Ints4 UTSW 7 97,134,178 (GRCm39) missense probably damaging 1.00
R4980:Ints4 UTSW 7 97,151,057 (GRCm39) critical splice donor site probably null
R5029:Ints4 UTSW 7 97,158,981 (GRCm39) missense probably benign 0.06
R5306:Ints4 UTSW 7 97,158,885 (GRCm39) missense probably damaging 1.00
R6317:Ints4 UTSW 7 97,178,425 (GRCm39) nonsense probably null
R6961:Ints4 UTSW 7 97,190,397 (GRCm39) makesense probably null
R7026:Ints4 UTSW 7 97,168,361 (GRCm39) missense possibly damaging 0.50
R7156:Ints4 UTSW 7 97,184,493 (GRCm39) critical splice donor site probably null
R7205:Ints4 UTSW 7 97,184,433 (GRCm39) nonsense probably null
R7234:Ints4 UTSW 7 97,179,507 (GRCm39) missense probably benign 0.00
R7418:Ints4 UTSW 7 97,140,179 (GRCm39) missense probably benign 0.03
R7423:Ints4 UTSW 7 97,156,926 (GRCm39) missense probably damaging 1.00
R7462:Ints4 UTSW 7 97,155,335 (GRCm39) missense probably benign 0.11
R7658:Ints4 UTSW 7 97,178,460 (GRCm39) missense possibly damaging 0.90
R7947:Ints4 UTSW 7 97,148,792 (GRCm39) missense probably benign
R8114:Ints4 UTSW 7 97,165,732 (GRCm39) splice site probably null
R8426:Ints4 UTSW 7 97,150,239 (GRCm39) nonsense probably null
R8765:Ints4 UTSW 7 97,151,016 (GRCm39) missense possibly damaging 0.70
R8777:Ints4 UTSW 7 97,134,227 (GRCm39) missense probably damaging 1.00
R8777-TAIL:Ints4 UTSW 7 97,134,227 (GRCm39) missense probably damaging 1.00
R8944:Ints4 UTSW 7 97,183,593 (GRCm39) missense probably benign
R9001:Ints4 UTSW 7 97,190,276 (GRCm39) missense possibly damaging 0.51
R9057:Ints4 UTSW 7 97,158,987 (GRCm39) missense possibly damaging 0.90
R9315:Ints4 UTSW 7 97,156,840 (GRCm39) intron probably benign
Predicted Primers PCR Primer
(F):5'- CACCCATGTGGATAAGATGAGG -3'
(R):5'- ACAGCCAGGACAGTGTCAAG -3'

Sequencing Primer
(F):5'- GGGACAAAAGGCCCATATTAAGCATC -3'
(R):5'- CAGGACAGTGTCAAGCTGATCTTC -3'
Posted On 2017-12-04