|Institutional Source||Beutler Lab|
|Gene Name||Rho guanine nucleotide exchange factor (GEF) 28|
|Synonyms||RhoGEF, Rgnef, Rho specific exchange factor, p190RhoGEF, 9230110L08Rik, D13Bwg1089e, RIP2|
|Is this an essential gene?||Possibly essential (E-score: 0.739)|
|Stock #||R6209 (G1)|
|Chromosomal Location||97899469-98206439 bp(-) (GRCm38)|
|Type of Mutation||critical splice donor site (2 bp from exon)|
|DNA Base Change (assembly)||A to T at 97929409 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000105053 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000109426] [ENSMUST00000225884]|
|Predicted Effect||probably null
|Predicted Effect||noncoding transcript
|Predicted Effect||probably benign
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the Rho guanine nucleotide exchange factor family. The encoded protein interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a knock-out allele are born at lower than expected Mendelian ratios and exhibit a reduction in overall size that becomes negligible by 8 weeks of age. Mouse embryonic fibroblasts display defects in cell migration and focal adhesion formation. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Arhgef28||
(F):5'- TACCACAAGGCCTCGTTTATTTAG -3'
(R):5'- GACACATGTCACTTAACACGTC -3'
(F):5'- TAGATATTTAGGAGTACAGGGGACTC -3'
(R):5'- TCCCCTGGGATGCTCAC -3'