Mutagenetix > Incidental Mutation

Incidental Mutation 'R6258:Itgb4'

506490

Institutional Source

Beutler Lab

Gene Symbol

Itgb4

Ensembl Gene

ENSMUSG00000020758

Gene Name

integrin beta 4

Synonyms

CD104

MMRRC Submission

044375-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6258 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

115865556-115899238 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 115874983 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Tryptophan at position 447 (R447W)

Ref Sequence

ENSEMBL: ENSMUSP00000127604 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021107] [ENSMUST00000068981] [ENSMUST00000106458] [ENSMUST00000106460] [ENSMUST00000106461] [ENSMUST00000169928]

AlphaFold

A2A863

Predicted Effect

probably benign
Transcript: ENSMUST00000021107
AA Change: R447W

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000021107
Gene: ENSMUSG00000020758
AA Change: R447W

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000068981
AA Change: R447W

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000070811
Gene: ENSMUSG00000020758
AA Change: R447W

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1459	1540	1.79e-12	SMART
FN3	1572	1656	1.7e-13	SMART
low complexity region	1689	1702	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106458
AA Change: R447W

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000102066
Gene: ENSMUSG00000020758
AA Change: R447W

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106460
AA Change: R447W

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000102068
Gene: ENSMUSG00000020758
AA Change: R447W

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1512	1593	1.79e-12	SMART
FN3	1625	1709	1.7e-13	SMART
low complexity region	1742	1755	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106461
AA Change: R447W

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000102069
Gene: ENSMUSG00000020758
AA Change: R447W

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151691

Predicted Effect

probably benign
Transcript: ENSMUST00000169928
AA Change: R447W

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000127604
Gene: ENSMUSG00000020758
AA Change: R447W

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180782

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 95.7%

Validation Efficiency

99% (74/75)

MGI Phenotype

FUNCTION: Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations die shortly after birth with extensive detachment of the epidermis and other squamus epithelia. Stratified tissues lack hemidesmosomes and simple epithelia are also defective in adherence. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamtsl3	T	C	7: 82,178,191 (GRCm39)		probably null	Het
Alms1	A	T	6: 85,605,717 (GRCm39)	K2456*	probably null	Het
Alppl2	A	T	1: 87,016,184 (GRCm39)	M225K	probably damaging	Het
AU041133	A	G	10: 81,986,992 (GRCm39)	E215G	probably damaging	Het
Carmil3	T	A	14: 55,737,889 (GRCm39)	L815Q	probably damaging	Het
Casr	A	G	16: 36,337,971 (GRCm39)	C60R	probably damaging	Het
Cdc7	A	G	5: 107,117,093 (GRCm39)	K84E	probably damaging	Het
Cdc73	G	A	1: 143,567,211 (GRCm39)	T104I	probably benign	Het
Clcc1	G	A	3: 108,580,624 (GRCm39)	V313I	possibly damaging	Het
Cntn3	A	G	6: 102,254,178 (GRCm39)		probably null	Het
Crocc2	A	G	1: 93,141,360 (GRCm39)	K1171R	possibly damaging	Het
Ctsa	T	C	2: 164,676,281 (GRCm39)	V86A	probably damaging	Het
Cyp2s1	ACAGCAGCAGCAGCAGCAGCAGCAG	ACAGCAGCAGCAGCAGCAGCAG	7: 25,515,867 (GRCm39)		probably benign	Het
Dab1	C	T	4: 104,588,948 (GRCm39)	A524V	probably benign	Het
Dnah17	C	A	11: 118,017,148 (GRCm39)	W197C	probably damaging	Het
Dnah17	A	T	11: 118,017,150 (GRCm39)	W197R	probably damaging	Het
Dnah17	C	T	11: 118,017,149 (GRCm39)	W197*	probably null	Het
Egflam	T	A	15: 7,263,773 (GRCm39)	T726S	probably damaging	Het
Eml2	T	G	7: 18,913,289 (GRCm39)		probably null	Het
Ercc6	T	A	14: 32,279,813 (GRCm39)	D609E	probably benign	Het
Erg	C	A	16: 95,181,100 (GRCm39)	R147L	probably damaging	Het
Faiml	T	C	9: 99,114,513 (GRCm39)	I125M	possibly damaging	Het
Fbxo41	A	T	6: 85,455,537 (GRCm39)	L549H	probably damaging	Het
Fbxw2	A	T	2: 34,702,825 (GRCm39)		probably null	Het
Fgd6	T	A	10: 93,880,161 (GRCm39)	N338K	probably benign	Het
Gaa	C	A	11: 119,171,997 (GRCm39)	A700D	probably benign	Het
Gm32742	T	A	9: 51,068,862 (GRCm39)	I200F	probably damaging	Het
Gm4924	T	G	10: 82,213,307 (GRCm39)		probably benign	Het
Gm8369	G	A	19: 11,488,973 (GRCm39)	A87T	possibly damaging	Het
H2-M10.1	T	A	17: 36,634,994 (GRCm39)	I304F	unknown	Het
Ighv5-8	A	G	12: 113,618,611 (GRCm39)	T9A	possibly damaging	Het
Jakmip1	G	T	5: 37,299,104 (GRCm39)	E775*	probably null	Het
Klhl40	T	C	9: 121,607,026 (GRCm39)	F62S	probably damaging	Het
Krtcap3	A	T	5: 31,409,572 (GRCm39)	R84W	probably damaging	Het
Lgr6	C	T	1: 134,921,748 (GRCm39)	A199T	probably damaging	Het
Lins1	T	C	7: 66,360,496 (GRCm39)		probably null	Het
Magi3	A	G	3: 103,996,912 (GRCm39)	L211P	probably damaging	Het
Map2k5	T	A	9: 63,124,647 (GRCm39)	I359F	probably benign	Het
Map4k5	C	A	12: 69,878,336 (GRCm39)	R355L	probably benign	Het
Mef2c	T	A	13: 83,801,057 (GRCm39)	D252E	probably damaging	Het
Methig1	T	C	15: 100,251,422 (GRCm39)	V111A	possibly damaging	Het
Mical3	A	T	6: 120,985,991 (GRCm39)	L150Q	probably damaging	Het
Nf1	A	T	11: 79,456,581 (GRCm39)		probably null	Het
Nisch	T	A	14: 30,899,085 (GRCm39)		probably benign	Het
Or4f15	C	T	2: 111,814,396 (GRCm39)	V8I	probably benign	Het
Or5p61	T	C	7: 107,758,181 (GRCm39)	N300D	probably damaging	Het
Pcdhb12	T	C	18: 37,569,892 (GRCm39)	V346A	probably benign	Het
Pde7b	T	C	10: 20,316,546 (GRCm39)	D168G	possibly damaging	Het
Pdzrn4	A	T	15: 92,655,562 (GRCm39)	E485V	probably damaging	Het
Pla2g4a	A	G	1: 149,733,238 (GRCm39)	S504P	probably benign	Het
Plin2	G	T	4: 86,575,526 (GRCm39)	A341D	probably damaging	Het
Psma8	A	G	18: 14,854,324 (GRCm39)	D68G	probably damaging	Het
Rcor3	G	A	1: 191,808,559 (GRCm39)	H207Y	probably benign	Het
Rptn	C	G	3: 93,305,437 (GRCm39)	H923Q	possibly damaging	Het
Ryr3	A	G	2: 112,490,449 (GRCm39)	F3795S	probably damaging	Het
Samm50	C	G	15: 84,084,512 (GRCm39)	P150A	probably damaging	Het
Samm50	C	A	15: 84,084,513 (GRCm39)	P150H	probably damaging	Het
Slc28a2b	A	T	2: 122,353,963 (GRCm39)	I530F	probably damaging	Het
Slc6a18	A	T	13: 73,818,164 (GRCm39)	C284*	probably null	Het
Smc3	T	A	19: 53,616,162 (GRCm39)		probably null	Het
Snrnp200	G	A	2: 127,060,343 (GRCm39)	G529D	possibly damaging	Het
Sord	T	A	2: 122,089,613 (GRCm39)		probably null	Het
Spdl1	T	A	11: 34,710,713 (GRCm39)	N345I	probably damaging	Het
Sucnr1	T	C	3: 59,993,778 (GRCm39)	L102P	probably damaging	Het
Tbc1d9	T	C	8: 83,937,145 (GRCm39)	W76R	probably damaging	Het
Tcerg1	T	A	18: 42,686,530 (GRCm39)	Y696N	probably damaging	Het
Thsd7b	A	G	1: 129,595,655 (GRCm39)	T492A	probably benign	Het
Trdmt1	T	A	2: 13,524,870 (GRCm39)	Q195L	probably benign	Het
Ubr3	A	G	2: 69,813,208 (GRCm39)		probably null	Het
Ung	A	T	5: 114,275,361 (GRCm39)	Y250F	probably benign	Het
Vezf1	A	G	11: 87,972,326 (GRCm39)	N229S	probably damaging	Het
Wdfy3	C	T	5: 102,020,831 (GRCm39)	R2491Q	possibly damaging	Het
Wdr97	C	A	15: 76,239,895 (GRCm39)	S277*	probably null	Het
Zfp1007	T	C	5: 109,824,433 (GRCm39)	H339R	probably benign	Het
Zfp709	TCGACG	TCG	8: 72,644,552 (GRCm39)		probably benign	Het
Zscan4-ps1	C	A	7: 10,799,829 (GRCm39)	E353D	probably benign	Het

Other mutations in Itgb4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01360:Itgb4	APN	11	115,881,766 (GRCm39)	missense	probably damaging	1.00
IGL01391:Itgb4	APN	11	115,881,746 (GRCm39)	missense	probably damaging	1.00
IGL01431:Itgb4	APN	11	115,897,283 (GRCm39)	splice site	probably benign
IGL01750:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01752:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01756:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01766:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01769:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02188:Itgb4	APN	11	115,894,213 (GRCm39)	missense	probably benign	0.08
IGL02262:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02293:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02318:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02319:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02338:Itgb4	APN	11	115,898,795 (GRCm39)	missense	probably damaging	1.00
IGL02734:Itgb4	APN	11	115,896,792 (GRCm39)	missense	probably benign
IGL02879:Itgb4	APN	11	115,885,178 (GRCm39)	missense	probably benign	0.05
IGL02889:Itgb4	APN	11	115,879,731 (GRCm39)	missense	probably damaging	1.00
IGL03183:Itgb4	APN	11	115,879,550 (GRCm39)	missense	probably damaging	1.00
IGL03054:Itgb4	UTSW	11	115,891,166 (GRCm39)	nonsense	probably null
R0021:Itgb4	UTSW	11	115,870,453 (GRCm39)	missense	possibly damaging	0.95
R0092:Itgb4	UTSW	11	115,869,950 (GRCm39)	missense	probably damaging	1.00
R0305:Itgb4	UTSW	11	115,870,238 (GRCm39)	missense	probably damaging	1.00
R0408:Itgb4	UTSW	11	115,898,428 (GRCm39)	missense	probably damaging	0.99
R0465:Itgb4	UTSW	11	115,870,582 (GRCm39)	missense	probably damaging	1.00
R0499:Itgb4	UTSW	11	115,870,521 (GRCm39)	missense	probably benign	0.00
R0535:Itgb4	UTSW	11	115,881,835 (GRCm39)	missense	possibly damaging	0.86
R0571:Itgb4	UTSW	11	115,870,594 (GRCm39)	missense	possibly damaging	0.94
R0613:Itgb4	UTSW	11	115,884,168 (GRCm39)	missense	probably damaging	0.98
R0838:Itgb4	UTSW	11	115,888,988 (GRCm39)	intron	probably benign
R1381:Itgb4	UTSW	11	115,885,163 (GRCm39)	missense	probably benign	0.00
R1451:Itgb4	UTSW	11	115,881,710 (GRCm39)	missense	probably damaging	1.00
R1459:Itgb4	UTSW	11	115,869,937 (GRCm39)	missense	probably benign	0.42
R1460:Itgb4	UTSW	11	115,874,990 (GRCm39)	missense	probably damaging	0.96
R1473:Itgb4	UTSW	11	115,874,873 (GRCm39)	missense	probably benign	0.01
R1484:Itgb4	UTSW	11	115,890,625 (GRCm39)	missense	probably benign	0.01
R1593:Itgb4	UTSW	11	115,871,817 (GRCm39)	missense	probably damaging	1.00
R1623:Itgb4	UTSW	11	115,882,142 (GRCm39)	nonsense	probably null
R1633:Itgb4	UTSW	11	115,898,586 (GRCm39)	missense	probably damaging	1.00
R1642:Itgb4	UTSW	11	115,898,183 (GRCm39)	missense	probably damaging	1.00
R1669:Itgb4	UTSW	11	115,882,156 (GRCm39)	missense	probably benign	0.07
R1713:Itgb4	UTSW	11	115,894,315 (GRCm39)	missense	probably damaging	1.00
R1732:Itgb4	UTSW	11	115,879,744 (GRCm39)	missense	probably damaging	1.00
R1791:Itgb4	UTSW	11	115,879,346 (GRCm39)	missense	probably damaging	1.00
R1847:Itgb4	UTSW	11	115,874,590 (GRCm39)	missense	probably benign	0.31
R1902:Itgb4	UTSW	11	115,871,564 (GRCm39)	missense	probably damaging	0.98
R1945:Itgb4	UTSW	11	115,884,279 (GRCm39)	nonsense	probably null
R2102:Itgb4	UTSW	11	115,896,561 (GRCm39)	missense	probably benign	0.23
R2184:Itgb4	UTSW	11	115,870,450 (GRCm39)	missense	probably damaging	0.96
R2334:Itgb4	UTSW	11	115,884,261 (GRCm39)	missense	probably damaging	1.00
R2401:Itgb4	UTSW	11	115,897,389 (GRCm39)	missense	possibly damaging	0.67
R3743:Itgb4	UTSW	11	115,894,496 (GRCm39)	missense	probably damaging	1.00
R3938:Itgb4	UTSW	11	115,896,752 (GRCm39)	missense	possibly damaging	0.92
R4134:Itgb4	UTSW	11	115,897,296 (GRCm39)	missense	probably benign	0.03
R4280:Itgb4	UTSW	11	115,881,761 (GRCm39)	missense	probably damaging	1.00
R4342:Itgb4	UTSW	11	115,879,555 (GRCm39)	missense	probably benign	0.01
R4434:Itgb4	UTSW	11	115,890,640 (GRCm39)	missense	probably benign	0.10
R4505:Itgb4	UTSW	11	115,874,087 (GRCm39)	splice site	silent
R4585:Itgb4	UTSW	11	115,884,151 (GRCm39)	missense	probably damaging	1.00
R4586:Itgb4	UTSW	11	115,884,151 (GRCm39)	missense	probably damaging	1.00
R4601:Itgb4	UTSW	11	115,896,548 (GRCm39)	missense	probably damaging	1.00
R4921:Itgb4	UTSW	11	115,897,431 (GRCm39)	missense	probably benign	0.12
R4962:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5027:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5029:Itgb4	UTSW	11	115,879,417 (GRCm39)	intron	probably benign
R5084:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5085:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5124:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5125:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5150:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5175:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5176:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5179:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5207:Itgb4	UTSW	11	115,897,365 (GRCm39)	missense	probably damaging	1.00
R5263:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5264:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5334:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5337:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5344:Itgb4	UTSW	11	115,880,575 (GRCm39)	missense	probably null	0.92
R5391:Itgb4	UTSW	11	115,875,894 (GRCm39)	missense	probably benign	0.05
R5437:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5440:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5653:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5654:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5655:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5772:Itgb4	UTSW	11	115,879,258 (GRCm39)	intron	probably benign
R5812:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5813:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5814:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5863:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5864:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5865:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5951:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5954:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5982:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6043:Itgb4	UTSW	11	115,870,212 (GRCm39)	missense	probably benign	0.30
R6133:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6134:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6135:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6169:Itgb4	UTSW	11	115,885,102 (GRCm39)	missense	probably damaging	0.98
R6172:Itgb4	UTSW	11	115,891,237 (GRCm39)	missense	probably benign	0.23
R6255:Itgb4	UTSW	11	115,888,963 (GRCm39)	missense	possibly damaging	0.83
R6259:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6260:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6612:Itgb4	UTSW	11	115,874,897 (GRCm39)	missense	probably benign	0.00
R7037:Itgb4	UTSW	11	115,896,391 (GRCm39)	nonsense	probably null
R7371:Itgb4	UTSW	11	115,888,906 (GRCm39)	missense	probably benign	0.29
R7605:Itgb4	UTSW	11	115,897,302 (GRCm39)	missense	probably benign	0.01
R7659:Itgb4	UTSW	11	115,870,557 (GRCm39)	missense	probably damaging	1.00
R7759:Itgb4	UTSW	11	115,894,536 (GRCm39)	missense	possibly damaging	0.92
R7804:Itgb4	UTSW	11	115,894,510 (GRCm39)	missense	probably damaging	1.00
R7832:Itgb4	UTSW	11	115,891,087 (GRCm39)	missense	probably damaging	1.00
R7842:Itgb4	UTSW	11	115,873,531 (GRCm39)	missense	probably benign	0.18
R7923:Itgb4	UTSW	11	115,873,525 (GRCm39)	critical splice acceptor site	probably null
R8004:Itgb4	UTSW	11	115,873,531 (GRCm39)	missense	probably benign	0.00
R8143:Itgb4	UTSW	11	115,884,255 (GRCm39)	missense	probably damaging	1.00
R8427:Itgb4	UTSW	11	115,882,544 (GRCm39)	critical splice donor site	probably null
R8857:Itgb4	UTSW	11	115,871,853 (GRCm39)	missense	probably benign	0.04
R8863:Itgb4	UTSW	11	115,875,898 (GRCm39)	nonsense	probably null
R8932:Itgb4	UTSW	11	115,879,295 (GRCm39)	missense	probably benign	0.01
R9153:Itgb4	UTSW	11	115,874,879 (GRCm39)	missense	probably benign	0.00
R9207:Itgb4	UTSW	11	115,897,923 (GRCm39)	missense	probably damaging	1.00
R9239:Itgb4	UTSW	11	115,898,130 (GRCm39)	missense	probably damaging	1.00
R9267:Itgb4	UTSW	11	115,870,465 (GRCm39)	missense	probably benign
R9289:Itgb4	UTSW	11	115,885,187 (GRCm39)	missense	probably benign	0.01
R9328:Itgb4	UTSW	11	115,880,625 (GRCm39)	missense	probably benign	0.00
R9435:Itgb4	UTSW	11	115,895,855 (GRCm39)	missense	probably benign	0.01
R9450:Itgb4	UTSW	11	115,874,097 (GRCm39)	missense	probably damaging	1.00
R9649:Itgb4	UTSW	11	115,885,171 (GRCm39)	missense	possibly damaging	0.78
R9779:Itgb4	UTSW	11	115,882,485 (GRCm39)	missense	probably damaging	1.00
X0062:Itgb4	UTSW	11	115,884,278 (GRCm39)	missense	probably damaging	1.00
Z1176:Itgb4	UTSW	11	115,897,346 (GRCm39)	missense	probably damaging	1.00
Z1177:Itgb4	UTSW	11	115,888,884 (GRCm39)	missense	probably benign	0.00
Z1177:Itgb4	UTSW	11	115,877,637 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CCAAGTCCCTGTCCATACACTG -3'
(R):5'- ACTGGACACTTCTGCTGCTC -3'

Sequencing Primer
(F):5'- TGAGCGAACAGGCTAATGTC -3'
(R):5'- CTCTCTGTGACTTGGGACAGC -3'

Posted On

2018-03-15