Incidental Mutation 'R6287:Zfp74'
ID 508267
Institutional Source Beutler Lab
Gene Symbol Zfp74
Ensembl Gene ENSMUSG00000059975
Gene Name zinc finger protein 74
Synonyms KRAB8, 2810054M15Rik, Zfp66
MMRRC Submission 044457-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.097) question?
Stock # R6287 (G1)
Quality Score 225.009
Status Validated
Chromosome 7
Chromosomal Location 29632086-29653579 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 29635201 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Isoleucine at position 169 (T169I)
Ref Sequence ENSEMBL: ENSMUSP00000103840 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000032797] [ENSMUST00000108205] [ENSMUST00000108211] [ENSMUST00000108212]
AlphaFold Q80W31
Predicted Effect probably benign
Transcript: ENSMUST00000032797
AA Change: T169I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000032797
Gene: ENSMUSG00000059975
AA Change: T169I

DomainStartEndE-ValueType
KRAB 8 68 5.02e-38 SMART
ZnF_C2H2 179 201 1.84e-4 SMART
ZnF_C2H2 207 229 2.36e-2 SMART
ZnF_C2H2 235 257 1.5e-4 SMART
ZnF_C2H2 263 285 2.79e-4 SMART
ZnF_C2H2 291 313 3.49e-5 SMART
ZnF_C2H2 319 341 6.42e-4 SMART
ZnF_C2H2 347 369 5.59e-4 SMART
ZnF_C2H2 375 397 5.21e-4 SMART
ZnF_C2H2 403 425 3.63e-3 SMART
ZnF_C2H2 431 453 1.82e-3 SMART
ZnF_C2H2 459 481 1.58e-3 SMART
ZnF_C2H2 487 509 2.12e-4 SMART
ZnF_C2H2 515 537 1.82e-3 SMART
ZnF_C2H2 543 565 2.24e-3 SMART
ZnF_C2H2 571 593 8.02e-5 SMART
ZnF_C2H2 599 621 1.04e-3 SMART
ZnF_C2H2 627 649 1.04e-3 SMART
ZnF_C2H2 655 677 9.88e-5 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000108205
AA Change: T169I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000103840
Gene: ENSMUSG00000059975
AA Change: T169I

DomainStartEndE-ValueType
KRAB 8 68 5.02e-38 SMART
ZnF_C2H2 179 201 1.84e-4 SMART
ZnF_C2H2 207 229 2.36e-2 SMART
ZnF_C2H2 235 257 1.5e-4 SMART
ZnF_C2H2 263 285 2.79e-4 SMART
ZnF_C2H2 291 313 3.49e-5 SMART
ZnF_C2H2 319 341 6.42e-4 SMART
ZnF_C2H2 347 369 5.59e-4 SMART
ZnF_C2H2 375 397 5.21e-4 SMART
ZnF_C2H2 403 425 3.63e-3 SMART
ZnF_C2H2 431 453 1.82e-3 SMART
ZnF_C2H2 459 481 1.58e-3 SMART
ZnF_C2H2 487 509 2.12e-4 SMART
ZnF_C2H2 515 537 1.82e-3 SMART
ZnF_C2H2 543 565 2.24e-3 SMART
ZnF_C2H2 571 593 8.02e-5 SMART
ZnF_C2H2 599 621 1.04e-3 SMART
ZnF_C2H2 627 649 1.04e-3 SMART
ZnF_C2H2 655 677 9.88e-5 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000108211
SMART Domains Protein: ENSMUSP00000103846
Gene: ENSMUSG00000059975

DomainStartEndE-ValueType
KRAB 8 68 5.02e-38 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000108212
SMART Domains Protein: ENSMUSP00000103847
Gene: ENSMUSG00000059975

DomainStartEndE-ValueType
KRAB 8 68 5.02e-38 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000149793
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.7%
  • 10x: 98.6%
  • 20x: 96.3%
Validation Efficiency 94% (34/36)
Allele List at MGI
Other mutations in this stock
Total: 35 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adcy7 A G 8: 89,037,736 (GRCm39) N268S possibly damaging Het
Ahnak T A 19: 8,992,367 (GRCm39) H4550Q probably benign Het
C330018D20Rik A C 18: 57,095,407 (GRCm39) probably null Het
Camta2 G C 11: 70,572,295 (GRCm39) Q310E probably damaging Het
Caskin1 A T 17: 24,715,683 (GRCm39) K149M probably damaging Het
Chml T C 1: 175,514,569 (GRCm39) K108E probably benign Het
Ddx60 T C 8: 62,403,612 (GRCm39) L345P probably damaging Het
Defb6 T A 8: 19,278,085 (GRCm39) C52* probably null Het
Flii A T 11: 60,612,423 (GRCm39) I288N probably damaging Het
Fmnl2 T C 2: 52,904,860 (GRCm39) Y55H probably damaging Het
Galk2 A T 2: 125,712,268 (GRCm39) probably benign Het
Ift140 T A 17: 25,269,408 (GRCm39) C688S probably benign Het
Lmln T C 16: 32,894,555 (GRCm39) probably null Het
Muc16 T C 9: 18,570,330 (GRCm39) T730A unknown Het
Nup88 T C 11: 70,856,581 (GRCm39) E184G probably benign Het
Omt2b T C 9: 78,235,543 (GRCm39) F27L possibly damaging Het
Or10g3 A T 14: 52,609,748 (GRCm39) V254E probably damaging Het
Or2a7 A G 6: 43,151,369 (GRCm39) I150V probably benign Het
Or2y13 A G 11: 49,415,072 (GRCm39) N174S probably damaging Het
Or4a15 G A 2: 89,193,363 (GRCm39) R137* probably null Het
Or6c69b T C 10: 129,627,254 (GRCm39) E68G probably damaging Het
Peg10 T TCCC 6: 4,756,451 (GRCm39) probably benign Het
Pigf G A 17: 87,304,967 (GRCm39) A192V probably damaging Het
Pikfyve T C 1: 65,292,691 (GRCm39) probably null Het
Ppat A T 5: 77,066,061 (GRCm39) Y352* probably null Het
Rapgef6 T G 11: 54,517,164 (GRCm39) probably null Het
Rrp15 A T 1: 186,481,373 (GRCm39) S45T probably benign Het
Sap18 A G 14: 58,035,771 (GRCm39) E30G probably damaging Het
Snx6 G T 12: 54,793,813 (GRCm39) A284E possibly damaging Het
Tm4sf4 T A 3: 57,333,112 (GRCm39) I26N probably damaging Het
Traf5 A G 1: 191,731,833 (GRCm39) L336P probably damaging Het
Ttl G A 2: 128,931,041 (GRCm39) A335T probably damaging Het
Vmn2r87 C T 10: 130,314,291 (GRCm39) probably null Het
Zfp937 A T 2: 150,080,261 (GRCm39) H97L possibly damaging Het
Zzef1 G T 11: 72,813,938 (GRCm39) E2842D probably damaging Het
Other mutations in Zfp74
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0355:Zfp74 UTSW 7 29,653,466 (GRCm39) start gained probably benign
R0387:Zfp74 UTSW 7 29,634,179 (GRCm39) missense probably benign 0.05
R0948:Zfp74 UTSW 7 29,635,362 (GRCm39) critical splice donor site probably null
R1757:Zfp74 UTSW 7 29,634,486 (GRCm39) missense probably benign 0.01
R1813:Zfp74 UTSW 7 29,634,569 (GRCm39) missense probably damaging 1.00
R1893:Zfp74 UTSW 7 29,635,470 (GRCm39) critical splice acceptor site probably null
R1896:Zfp74 UTSW 7 29,634,569 (GRCm39) missense probably damaging 1.00
R1958:Zfp74 UTSW 7 29,635,136 (GRCm39) missense probably benign 0.08
R2092:Zfp74 UTSW 7 29,653,349 (GRCm39) start gained probably benign
R2111:Zfp74 UTSW 7 29,634,443 (GRCm39) nonsense probably null
R4894:Zfp74 UTSW 7 29,635,470 (GRCm39) critical splice acceptor site probably benign
R5121:Zfp74 UTSW 7 29,631,932 (GRCm39) splice site probably null
R5123:Zfp74 UTSW 7 29,634,158 (GRCm39) missense probably damaging 1.00
R5129:Zfp74 UTSW 7 29,631,880 (GRCm39) missense probably benign 0.00
R5213:Zfp74 UTSW 7 29,634,668 (GRCm39) missense probably damaging 1.00
R5460:Zfp74 UTSW 7 29,635,316 (GRCm39) missense probably benign 0.04
R5519:Zfp74 UTSW 7 29,634,559 (GRCm39) missense probably damaging 0.99
R5589:Zfp74 UTSW 7 29,633,990 (GRCm39) missense probably damaging 1.00
R6330:Zfp74 UTSW 7 29,637,412 (GRCm39) missense probably damaging 1.00
R6370:Zfp74 UTSW 7 29,631,835 (GRCm39) missense probably damaging 0.96
R6407:Zfp74 UTSW 7 29,635,048 (GRCm39) missense probably damaging 1.00
R6694:Zfp74 UTSW 7 29,634,559 (GRCm39) missense probably damaging 0.99
R6791:Zfp74 UTSW 7 29,633,860 (GRCm39) missense probably benign 0.02
R7144:Zfp74 UTSW 7 29,634,590 (GRCm39) missense probably damaging 0.98
R7662:Zfp74 UTSW 7 29,653,278 (GRCm39) critical splice donor site probably null
R7667:Zfp74 UTSW 7 29,634,608 (GRCm39) missense probably damaging 1.00
R7898:Zfp74 UTSW 7 29,635,380 (GRCm39) nonsense probably null
R7940:Zfp74 UTSW 7 29,631,867 (GRCm39) missense probably benign 0.07
R8676:Zfp74 UTSW 7 29,634,079 (GRCm39) missense probably damaging 1.00
R8864:Zfp74 UTSW 7 29,634,235 (GRCm39) missense probably damaging 1.00
R8940:Zfp74 UTSW 7 29,634,772 (GRCm39) missense possibly damaging 0.96
R9748:Zfp74 UTSW 7 29,634,751 (GRCm39) missense probably damaging 1.00
R9764:Zfp74 UTSW 7 29,631,845 (GRCm39) missense probably benign 0.11
Predicted Primers PCR Primer
(F):5'- CCTCATATGCAGAGCAAGGG -3'
(R):5'- CGGAGGAATTTGCAAGTACG -3'

Sequencing Primer
(F):5'- AACTTTCTGATGTGCGACAAGGC -3'
(R):5'- GAGGAATTTGCAAGTACGTTTTTCCC -3'
Posted On 2018-03-15