Incidental Mutation 'R6301:Olfr165'
ID509124
Institutional Source Beutler Lab
Gene Symbol Olfr165
Ensembl Gene ENSMUSG00000050158
Gene Nameolfactory receptor 165
SynonymsMOR279-1, GA_x54KRFPKG5P-15855164-15854223
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.079) question?
Stock #R6301 (G1)
Quality Score225.009
Status Validated
Chromosome16
Chromosomal Location19406244-19423134 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 19407417 bp
ZygosityHeterozygous
Amino Acid Change Phenylalanine to Isoleucine at position 200 (F200I)
Ref Sequence ENSEMBL: ENSMUSP00000145668 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000052516] [ENSMUST00000206110] [ENSMUST00000206410]
Predicted Effect possibly damaging
Transcript: ENSMUST00000052516
AA Change: F200I

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000062079
Gene: ENSMUSG00000050158
AA Change: F200I

DomainStartEndE-ValueType
Pfam:7tm_4 29 309 5.6e-48 PFAM
Pfam:7TM_GPCR_Srsx 36 306 6e-10 PFAM
Pfam:7tm_1 42 291 4e-21 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000103984
Predicted Effect possibly damaging
Transcript: ENSMUST00000206110
AA Change: F200I

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)
Predicted Effect possibly damaging
Transcript: ENSMUST00000206410
AA Change: F201I

PolyPhen 2 Score 0.814 (Sensitivity: 0.84; Specificity: 0.93)
Meta Mutation Damage Score 0.214 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.5%
  • 20x: 98.5%
Validation Efficiency 100% (66/66)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 61 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acot10 T C 15: 20,666,262 N131S probably benign Het
Agbl5 A G 5: 30,891,833 Y220C probably damaging Het
Ankdd1a C T 9: 65,508,061 A227T possibly damaging Het
Ap3b1 A T 13: 94,528,295 Q914L unknown Het
Arhgef25 T C 10: 127,185,882 D216G possibly damaging Het
Bcas2 T A 3: 103,171,871 probably benign Het
Bpifb5 A C 2: 154,230,219 H282P possibly damaging Het
Ccdc167 A G 17: 29,705,582 I15T probably damaging Het
Cd248 A G 19: 5,069,981 N619S probably benign Het
Chrna1 A G 2: 73,570,484 F234S possibly damaging Het
Clcc1 T G 3: 108,673,366 M332R probably damaging Het
Cmklr1 T A 5: 113,614,938 M1L possibly damaging Het
Cntnap5c A G 17: 57,892,037 M109V probably benign Het
Coq2 A G 5: 100,661,863 I18T possibly damaging Het
Crybg3 T C 16: 59,530,338 S880G probably damaging Het
Cubn A G 2: 13,478,078 C286R probably damaging Het
Defa24 T C 8: 21,735,283 V63A probably benign Het
Dnah6 C T 6: 73,086,217 R2634H probably damaging Het
Dusp8 T A 7: 142,083,019 probably null Het
Elac1 T C 18: 73,738,868 D352G probably damaging Het
Ermap A T 4: 119,185,603 V241E probably damaging Het
Fgf10 T A 13: 118,715,511 M43K probably benign Het
Gabrd A G 4: 155,387,267 V193A probably damaging Het
Gm13124 C T 4: 144,558,654 A138T probably damaging Het
Gm6588 A T 5: 112,450,468 I294F possibly damaging Het
Gm884 A T 11: 103,618,930 probably benign Het
Hectd4 T G 5: 121,254,220 C182W possibly damaging Het
Hook3 C T 8: 26,034,940 W26* probably null Het
Kif1a C A 1: 93,054,941 E714* probably null Het
Krt6b T C 15: 101,678,951 E236G probably damaging Het
Large2 A G 2: 92,369,516 L209P probably damaging Het
Lats1 A G 10: 7,703,107 N665S probably benign Het
Lrrtm3 T C 10: 64,089,222 I55M probably benign Het
Ltk A G 2: 119,751,757 S838P probably damaging Het
Ltn1 T C 16: 87,420,306 I348V probably benign Het
Mag T A 7: 30,900,679 S559C probably damaging Het
Mink1 A G 11: 70,612,294 H1072R possibly damaging Het
Mycbp2 T A 14: 103,155,426 Q833L probably damaging Het
Myh4 G A 11: 67,255,333 E1406K possibly damaging Het
Npc1 C T 18: 12,197,245 V950I probably benign Het
Npl A G 1: 153,518,881 probably null Het
Olfr1493-ps1 A G 19: 13,726,389 I43V probably benign Het
Olfr743 T A 14: 50,534,254 F281I probably benign Het
Olfr883 ATTGCTGTTT ATTGCTGTTTGCTGTTT 9: 38,026,540 probably null Het
Oxsm A T 14: 16,242,220 I183N probably damaging Het
Pde7a T C 3: 19,243,163 I108V probably benign Het
Pgghg A T 7: 140,946,376 T585S probably damaging Het
Pkhd1l1 G A 15: 44,589,525 D3949N probably damaging Het
Ralgapa2 A T 2: 146,327,411 H1777Q possibly damaging Het
Rela G A 19: 5,645,410 probably null Het
Rilpl1 C A 5: 124,514,539 G41W probably damaging Het
Rp1 T C 1: 4,347,254 K1212E probably benign Het
Sgca A C 11: 94,972,567 L28V probably damaging Het
Ssmem1 A G 6: 30,519,759 R148G probably damaging Het
St8sia5 A T 18: 77,246,140 N165Y probably damaging Het
Tbl2 A T 5: 135,159,369 H339L probably benign Het
Tcof1 G A 18: 60,828,825 P718L probably damaging Het
Trim72 A T 7: 128,004,614 E44V possibly damaging Het
Try10 T A 6: 41,355,589 S60T probably benign Het
Usp31 A T 7: 121,648,276 S1315T possibly damaging Het
Xrn2 A G 2: 147,063,342 I856V probably benign Het
Other mutations in Olfr165
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01353:Olfr165 APN 16 19407583 missense probably benign 0.03
IGL01355:Olfr165 APN 16 19407583 missense probably benign 0.03
IGL01363:Olfr165 APN 16 19407583 missense probably benign 0.03
IGL01467:Olfr165 APN 16 19407789 missense probably benign 0.24
IGL02267:Olfr165 APN 16 19407164 missense possibly damaging 0.80
R1194:Olfr165 UTSW 16 19407180 missense possibly damaging 0.75
R1624:Olfr165 UTSW 16 19407704 missense probably benign 0.06
R2248:Olfr165 UTSW 16 19407194 missense probably damaging 0.98
R4865:Olfr165 UTSW 16 19407301 missense probably damaging 0.98
R4988:Olfr165 UTSW 16 19407110 missense probably benign 0.00
R5384:Olfr165 UTSW 16 19407797 missense probably damaging 1.00
R5858:Olfr165 UTSW 16 19407225 missense possibly damaging 0.91
R5997:Olfr165 UTSW 16 19407944 missense probably benign 0.10
R6570:Olfr165 UTSW 16 19407318 missense probably benign 0.00
R6703:Olfr165 UTSW 16 19407372 missense probably benign 0.03
R6953:Olfr165 UTSW 16 19407528 missense probably benign 0.04
Predicted Primers PCR Primer
(F):5'- TGGCTGCCCCATAGTATATTAC -3'
(R):5'- GCAATGGCCTATGACCGTTATG -3'

Sequencing Primer
(F):5'- TAGTATATTACCACCACCATGACGTG -3'
(R):5'- AATCTGTAGTCTAATGGCTGCC -3'
Posted On2018-04-02