Mutagenetix > Incidental Mutation

Incidental Mutation 'R6329:Or8g24'

509400

Institutional Source

Beutler Lab

Gene Symbol

Or8g24

Ensembl Gene

ENSMUSG00000048501

Gene Name

olfactory receptor family 8 subfamily G member 24

Synonyms

MOR171-25, Olfr938, GA_x6K02T2PVTD-32774646-32773699

MMRRC Submission

044483-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R6329 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

38989092-38990039 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 38989199 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 281 (V281I)

Ref Sequence

ENSEMBL: ENSMUSP00000055053 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056499]

AlphaFold

Q9EQ93

Predicted Effect

probably benign
Transcript: ENSMUST00000056499
AA Change: V281I

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000055053
Gene: ENSMUSG00000048501
AA Change: V281I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	8e-49	PFAM
Pfam:7tm_1	41	290	5.5e-18	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000175411

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000215888

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930579F01Rik	T	C	3: 137,879,457 (GRCm39)	H72R	probably damaging	Het
Abca13	A	T	11: 9,227,937 (GRCm39)	N660I	probably damaging	Het
Actr8	C	T	14: 29,715,041 (GRCm39)	R619*	probably null	Het
Adam18	C	A	8: 25,104,843 (GRCm39)	G657V	probably damaging	Het
Adcyap1	A	G	17: 93,510,227 (GRCm39)	E85G	probably benign	Het
Akr1c14	A	G	13: 4,137,302 (GRCm39)	Y305C	probably damaging	Het
Ankdd1b	T	A	13: 96,591,388 (GRCm39)	H37L	possibly damaging	Het
Ap4e1	T	C	2: 126,903,636 (GRCm39)	L846P	probably benign	Het
Aqp9	A	T	9: 71,039,966 (GRCm39)	Y105*	probably null	Het
Arid1b	C	T	17: 5,387,538 (GRCm39)	Q1664*	probably null	Het
Aup1	A	G	6: 83,031,588 (GRCm39)		probably benign	Het
Bnip1	C	A	17: 27,005,684 (GRCm39)	S64*	probably null	Het
Calcr	T	A	6: 3,687,621 (GRCm39)	Q422L	probably damaging	Het
Ccdc162	T	A	10: 41,539,147 (GRCm39)	D407V	possibly damaging	Het
Ccdc66	G	T	14: 27,208,441 (GRCm39)	S760R	probably benign	Het
Cd22	T	A	7: 30,577,193 (GRCm39)	E38V	probably damaging	Het
Cggbp1	T	C	16: 64,676,383 (GRCm39)	Y150H	probably damaging	Het
Chd3	T	C	11: 69,252,510 (GRCm39)	K263R	possibly damaging	Het
Col4a2	T	A	8: 11,496,238 (GRCm39)	F1620I	probably damaging	Het
Col6a2	T	C	10: 76,435,662 (GRCm39)	T858A	probably benign	Het
Dnah7a	T	A	1: 53,580,273 (GRCm39)	D1554V	probably damaging	Het
Dnajc25	A	T	4: 59,013,678 (GRCm39)	Q132L	probably benign	Het
Dnase1l1	C	T	X: 73,320,644 (GRCm39)		probably null	Homo
Ehbp1l1	A	C	19: 5,768,795 (GRCm39)	I836S	possibly damaging	Het
Elf1	A	G	14: 79,810,779 (GRCm39)	Q288R	possibly damaging	Het
Fbn1	C	A	2: 125,150,393 (GRCm39)	V2608L	possibly damaging	Het
Frmpd1	T	C	4: 45,268,551 (GRCm39)	I232T	possibly damaging	Het
Fuca1	T	A	4: 135,662,137 (GRCm39)	I355N	probably damaging	Het
Gcnt4	A	G	13: 97,083,781 (GRCm39)	D359G	probably damaging	Het
Gm5134	T	C	10: 75,790,494 (GRCm39)	M30T	possibly damaging	Het
Grk1	T	G	8: 13,455,704 (GRCm39)	L196R	probably damaging	Het
Igkv14-126	A	C	6: 67,873,548 (GRCm39)	D92A	probably damaging	Het
Kmt2c	A	G	5: 25,520,600 (GRCm39)	S1837P	probably benign	Het
Lhx4	T	A	1: 155,578,300 (GRCm39)	T281S	probably benign	Het
Lrrc55	G	T	2: 85,026,653 (GRCm39)	H124N	probably benign	Het
Marchf8	T	A	6: 116,383,277 (GRCm39)	I566N	possibly damaging	Het
Mycbp2	G	A	14: 103,393,288 (GRCm39)	A3091V	probably benign	Het
Nlrp4b	A	G	7: 10,458,847 (GRCm39)	N355S	probably benign	Het
Nmur2	A	G	11: 55,920,411 (GRCm39)	V278A	probably benign	Het
Nod1	T	C	6: 54,921,689 (GRCm39)	M210V	probably benign	Het
Nt5c1b	T	A	12: 10,422,138 (GRCm39)	C63*	probably null	Het
Or1e1f	A	G	11: 73,855,568 (GRCm39)	I45V	possibly damaging	Het
Or1j17	A	T	2: 36,578,694 (GRCm39)	K227*	probably null	Het
Or4g7	C	T	2: 111,309,573 (GRCm39)	A148V	possibly damaging	Het
Or5d37	G	A	2: 87,924,008 (GRCm39)	P91S	probably damaging	Het
Or7g21	A	C	9: 19,032,253 (GRCm39)	M1L	probably benign	Het
Or8b37	T	C	9: 37,959,121 (GRCm39)	V201A	probably benign	Het
Osbp2	T	A	11: 3,665,153 (GRCm39)	S517C	probably damaging	Het
Pcdha1	C	T	18: 37,065,301 (GRCm39)	P655L	probably damaging	Het
Pdcd6	A	G	13: 74,452,098 (GRCm39)	Y181H	probably damaging	Het
Perp	G	A	10: 18,731,502 (GRCm39)	G154S	probably damaging	Het
Perp	G	T	10: 18,731,503 (GRCm39)	G154V	probably damaging	Het
Pira13	T	C	7: 3,825,850 (GRCm39)	T340A	probably damaging	Het
Prokr1	T	G	6: 87,558,774 (GRCm39)	T204P	possibly damaging	Het
Prpf3	A	T	3: 95,739,890 (GRCm39)	C630S	probably damaging	Het
Pter	C	A	2: 12,985,359 (GRCm39)	H230N	probably damaging	Het
Ptprs	A	T	17: 56,724,427 (GRCm39)	Y1167*	probably null	Het
Rad54l2	T	C	9: 106,595,121 (GRCm39)	I279V	possibly damaging	Het
Rora	T	A	9: 69,280,468 (GRCm39)	L347Q	probably damaging	Het
Runx1t1	T	G	4: 13,785,136 (GRCm39)	M1R	probably null	Het
Sdcbp	A	G	4: 6,381,064 (GRCm39)	S70G	probably benign	Het
Serpinb12	A	G	1: 106,881,493 (GRCm39)	Y210C	probably damaging	Het
Sipa1	T	C	19: 5,701,517 (GRCm39)	E1015G	probably damaging	Het
Slc15a2	A	C	16: 36,572,144 (GRCm39)	L740R	possibly damaging	Het
Specc1	A	G	11: 62,047,379 (GRCm39)	E916G	probably damaging	Het
Spta1	T	C	1: 174,041,743 (GRCm39)	I1371T	possibly damaging	Het
Tagln3	T	C	16: 45,533,365 (GRCm39)	M130V	probably benign	Het
Tchh	A	T	3: 93,353,752 (GRCm39)	E1064V	unknown	Het
Tdp1	A	T	12: 99,880,330 (GRCm39)	S464C	probably damaging	Het
Tdp1	G	C	12: 99,880,331 (GRCm39)	S464T	probably benign	Het
Tenm3	T	C	8: 48,729,884 (GRCm39)	Y1374C	probably damaging	Het
Tmprss3	A	T	17: 31,402,833 (GRCm39)	Y455*	probably null	Het
Ttc5	A	G	14: 51,003,385 (GRCm39)	V433A	possibly damaging	Het
Wnt5a	A	T	14: 28,240,449 (GRCm39)	R180*	probably null	Het
Zfp53	A	G	17: 21,728,372 (GRCm39)	D135G	probably benign	Het
Zfp619	T	A	7: 39,186,969 (GRCm39)	C1000S	probably damaging	Het
Zfp647	G	A	15: 76,796,285 (GRCm39)	P125L	probably damaging	Het

Other mutations in Or8g24

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01020:Or8g24	APN	9	38,989,747 (GRCm39)	missense	probably damaging	0.96
IGL01298:Or8g24	APN	9	38,990,020 (GRCm39)	missense	possibly damaging	0.63
IGL02930:Or8g24	APN	9	38,989,308 (GRCm39)	missense	probably damaging	1.00
IGL03346:Or8g24	APN	9	38,989,257 (GRCm39)	missense	probably benign	0.35
IGL03346:Or8g24	APN	9	38,989,258 (GRCm39)	missense	probably damaging	0.99
IGL03399:Or8g24	APN	9	38,989,533 (GRCm39)	nonsense	probably null
R0536:Or8g24	UTSW	9	38,989,625 (GRCm39)	missense	probably benign	0.03
R1170:Or8g24	UTSW	9	38,989,525 (GRCm39)	missense	possibly damaging	0.50
R1951:Or8g24	UTSW	9	38,989,580 (GRCm39)	missense	probably benign	0.07
R1952:Or8g24	UTSW	9	38,989,580 (GRCm39)	missense	probably benign	0.07
R2066:Or8g24	UTSW	9	38,989,510 (GRCm39)	missense	probably damaging	1.00
R2906:Or8g24	UTSW	9	38,989,669 (GRCm39)	missense	probably benign	0.39
R4707:Or8g24	UTSW	9	38,989,558 (GRCm39)	missense	probably benign	0.00
R4767:Or8g24	UTSW	9	38,989,988 (GRCm39)	missense	possibly damaging	0.71
R4951:Or8g24	UTSW	9	38,989,555 (GRCm39)	missense	probably benign	0.10
R5888:Or8g24	UTSW	9	38,989,263 (GRCm39)	nonsense	probably null
R5905:Or8g24	UTSW	9	38,989,379 (GRCm39)	missense	probably damaging	1.00
R6028:Or8g24	UTSW	9	38,989,379 (GRCm39)	missense	probably damaging	1.00
R7240:Or8g24	UTSW	9	38,989,906 (GRCm39)	missense	probably damaging	0.99
R7345:Or8g24	UTSW	9	38,989,630 (GRCm39)	missense	probably damaging	1.00
R8058:Or8g24	UTSW	9	38,989,862 (GRCm39)	missense	probably damaging	1.00
R9023:Or8g24	UTSW	9	38,989,307 (GRCm39)	missense	probably benign	0.09
R9547:Or8g24	UTSW	9	38,989,927 (GRCm39)	missense	probably damaging	0.99
R9682:Or8g24	UTSW	9	38,989,874 (GRCm39)	missense	possibly damaging	0.95
R9760:Or8g24	UTSW	9	38,989,271 (GRCm39)	missense	possibly damaging	0.95
X0062:Or8g24	UTSW	9	38,989,762 (GRCm39)	missense	probably benign	0.15

Predicted Primers

PCR Primer
(F):5'- GTCACACATAGAGTTATTAATGGCTGC -3'
(R):5'- TGTACCAGCTCTGACCATCC -3'

Sequencing Primer
(F):5'- GCTATAATCTATTGCCAAGGT -3'
(R):5'- GTACCAGCTCTGACCATCCTTAGC -3'

Posted On

2018-04-02