Incidental Mutation 'R6317:Vmn1r181'
ID 510028
Institutional Source Beutler Lab
Gene Symbol Vmn1r181
Ensembl Gene ENSMUSG00000097425
Gene Name vomeronasal 1 receptor 181
Synonyms V1rd20
MMRRC Submission 044417-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.055) question?
Stock # R6317 (G1)
Quality Score 225.009
Status Validated
Chromosome 7
Chromosomal Location 23683386-23684473 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 23684183 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Glutamine at position 216 (R216Q)
Ref Sequence ENSEMBL: ENSMUSP00000154403 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000181796] [ENSMUST00000226843] [ENSMUST00000226978] [ENSMUST00000227637] [ENSMUST00000228399] [ENSMUST00000228842]
AlphaFold Q0P547
Predicted Effect probably benign
Transcript: ENSMUST00000181796
AA Change: R216Q

PolyPhen 2 Score 0.117 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000137685
Gene: ENSMUSG00000097425
AA Change: R216Q

DomainStartEndE-ValueType
Pfam:TAS2R 8 296 3.9e-9 PFAM
Pfam:V1R 41 295 2.7e-13 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000226843
AA Change: R216Q

PolyPhen 2 Score 0.233 (Sensitivity: 0.91; Specificity: 0.88)
Predicted Effect probably benign
Transcript: ENSMUST00000226978
AA Change: R216Q

PolyPhen 2 Score 0.233 (Sensitivity: 0.91; Specificity: 0.88)
Predicted Effect probably benign
Transcript: ENSMUST00000227637
AA Change: R216Q

PolyPhen 2 Score 0.233 (Sensitivity: 0.91; Specificity: 0.88)
Predicted Effect probably benign
Transcript: ENSMUST00000228399
AA Change: R216Q

PolyPhen 2 Score 0.233 (Sensitivity: 0.91; Specificity: 0.88)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000228531
Predicted Effect noncoding transcript
Transcript: ENSMUST00000228607
Predicted Effect probably benign
Transcript: ENSMUST00000228842
AA Change: R216Q

PolyPhen 2 Score 0.233 (Sensitivity: 0.91; Specificity: 0.88)
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.4%
  • 20x: 98.2%
Validation Efficiency 100% (62/62)
Allele List at MGI
Other mutations in this stock
Total: 61 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930503L19Rik T C 18: 70,601,264 (GRCm39) N206S probably damaging Het
Abcf2 A T 5: 24,774,156 (GRCm39) Y315* probably null Het
Adck1 T G 12: 88,368,921 (GRCm39) V133G probably damaging Het
Aoc2 T C 11: 101,216,292 (GRCm39) F125S probably damaging Het
As3mt A G 19: 46,713,410 (GRCm39) D319G probably benign Het
Atp1a2 C G 1: 172,116,903 (GRCm39) R238P probably damaging Het
Baz1a T C 12: 55,001,585 (GRCm39) Q145R possibly damaging Het
Bhlhe22 A G 3: 18,109,778 (GRCm39) E276G probably damaging Het
Cdo1 C A 18: 46,861,104 (GRCm39) V36L probably benign Het
Ces1h A G 8: 94,084,046 (GRCm39) F388S unknown Het
Col6a5 T A 9: 105,766,266 (GRCm39) N1885Y probably damaging Het
Corin A T 5: 72,496,388 (GRCm39) C522S probably damaging Het
Csmd1 G T 8: 16,760,658 (GRCm39) T159K possibly damaging Het
Cspg4b T A 13: 113,504,802 (GRCm39) L1977H probably benign Het
Cwc27 T A 13: 104,940,769 (GRCm39) K197* probably null Het
Cyp20a1 T C 1: 60,391,283 (GRCm39) S26P probably damaging Het
Daxx T A 17: 34,130,949 (GRCm39) D321E probably damaging Het
Gria2 A C 3: 80,648,311 (GRCm39) Y142D possibly damaging Het
Gspt1 T C 16: 11,041,072 (GRCm39) probably null Het
Ighv1-80 A T 12: 115,876,265 (GRCm39) V17D probably damaging Het
Ints4 T A 7: 97,178,425 (GRCm39) L675* probably null Het
Kif13a C T 13: 46,980,233 (GRCm39) R173Q probably damaging Het
Map3k2 T C 18: 32,336,086 (GRCm39) I91T probably damaging Het
Map3k8 A G 18: 4,348,979 (GRCm39) probably null Het
Mcemp1 G A 8: 3,717,284 (GRCm39) W101* probably null Het
Naca T C 10: 127,879,993 (GRCm39) I1675T probably benign Het
Nol9 T C 4: 152,125,514 (GRCm39) F155S probably damaging Het
Obscn C A 11: 58,960,721 (GRCm39) D3406Y probably damaging Het
Obsl1 A G 1: 75,466,273 (GRCm39) V1485A possibly damaging Het
Oga A T 19: 45,760,119 (GRCm39) probably null Het
Or8a1 T C 9: 37,641,725 (GRCm39) K185E possibly damaging Het
Otog C A 7: 45,950,639 (GRCm39) P337H probably damaging Het
Patl1 T C 19: 11,898,242 (GRCm39) L140P probably damaging Het
Pcca G A 14: 122,820,035 (GRCm39) V60M probably damaging Het
Pex11g G T 8: 3,514,092 (GRCm39) D23E probably damaging Het
Phactr2 A T 10: 13,137,626 (GRCm39) M172K probably damaging Het
Plce1 G A 19: 38,512,974 (GRCm39) W91* probably null Het
Plch1 C T 3: 63,688,811 (GRCm39) W131* probably null Het
Podn A G 4: 107,884,357 (GRCm39) F44S probably damaging Het
Polr3e A G 7: 120,527,205 (GRCm39) D87G possibly damaging Het
Prmt2 T A 10: 76,058,351 (GRCm39) I153F probably benign Het
Prpf6 T C 2: 181,273,229 (GRCm39) V258A probably benign Het
Ptpn21 G T 12: 98,655,521 (GRCm39) A482E probably damaging Het
Qrich1 A T 9: 108,411,491 (GRCm39) N339Y probably damaging Het
Rabgap1 T A 2: 37,432,659 (GRCm39) V750D possibly damaging Het
Reg3d G A 6: 78,354,428 (GRCm39) P58S probably damaging Het
Rp1 A G 1: 4,112,212 (GRCm39) L1213P unknown Het
Sema6b A G 17: 56,431,047 (GRCm39) L872S probably benign Het
Serpinb7 T C 1: 107,379,436 (GRCm39) I281T probably damaging Het
Shank2 T C 7: 143,838,821 (GRCm39) V685A possibly damaging Het
Slc28a2 A G 2: 122,284,980 (GRCm39) I323V possibly damaging Het
Slc7a6 A T 8: 106,919,099 (GRCm39) I228F probably damaging Het
Slc9a9 A G 9: 94,821,512 (GRCm39) T300A possibly damaging Het
Spta1 A G 1: 174,068,653 (GRCm39) N2151S probably damaging Het
Sult2a1 T A 7: 13,569,945 (GRCm39) I96L probably benign Het
Tgm2 T C 2: 157,966,070 (GRCm39) D528G probably benign Het
Ubl7 T C 9: 57,818,456 (GRCm39) probably null Het
Vcan T A 13: 89,839,716 (GRCm39) I983L probably benign Het
Vmn1r172 T C 7: 23,359,742 (GRCm39) L209P probably damaging Het
Vmn1r3 T C 4: 3,184,993 (GRCm39) S105G probably benign Het
Zfp644 A T 5: 106,783,711 (GRCm39) H945Q probably damaging Het
Other mutations in Vmn1r181
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01443:Vmn1r181 APN 7 23,684,006 (GRCm39) missense possibly damaging 0.91
IGL02055:Vmn1r181 APN 7 23,683,978 (GRCm39) missense probably damaging 1.00
IGL02444:Vmn1r181 APN 7 23,683,948 (GRCm39) missense probably damaging 1.00
IGL03012:Vmn1r181 APN 7 23,684,027 (GRCm39) missense probably damaging 0.98
IGL03026:Vmn1r181 APN 7 23,684,000 (GRCm39) missense possibly damaging 0.94
R0255:Vmn1r181 UTSW 7 23,683,759 (GRCm39) missense probably benign 0.02
R1481:Vmn1r181 UTSW 7 23,684,137 (GRCm39) missense probably damaging 1.00
R2847:Vmn1r181 UTSW 7 23,683,943 (GRCm39) missense possibly damaging 0.88
R2848:Vmn1r181 UTSW 7 23,683,943 (GRCm39) missense possibly damaging 0.88
R2849:Vmn1r181 UTSW 7 23,683,943 (GRCm39) missense possibly damaging 0.88
R3441:Vmn1r181 UTSW 7 23,684,308 (GRCm39) missense probably benign 0.05
R3757:Vmn1r181 UTSW 7 23,683,909 (GRCm39) missense possibly damaging 0.90
R3945:Vmn1r181 UTSW 7 23,683,577 (GRCm39) missense probably damaging 0.98
R3983:Vmn1r181 UTSW 7 23,684,234 (GRCm39) missense probably benign 0.23
R4780:Vmn1r181 UTSW 7 23,684,008 (GRCm39) missense possibly damaging 0.64
R4999:Vmn1r181 UTSW 7 23,683,790 (GRCm39) missense probably damaging 0.99
R5463:Vmn1r181 UTSW 7 23,683,787 (GRCm39) missense probably benign 0.02
R5672:Vmn1r181 UTSW 7 23,683,741 (GRCm39) missense probably damaging 1.00
R6229:Vmn1r181 UTSW 7 23,683,580 (GRCm39) missense probably damaging 1.00
R6315:Vmn1r181 UTSW 7 23,684,183 (GRCm39) missense probably benign 0.23
R6316:Vmn1r181 UTSW 7 23,684,183 (GRCm39) missense probably benign 0.23
R6324:Vmn1r181 UTSW 7 23,684,183 (GRCm39) missense probably benign 0.23
R6326:Vmn1r181 UTSW 7 23,684,183 (GRCm39) missense probably benign 0.23
R6988:Vmn1r181 UTSW 7 23,684,272 (GRCm39) missense probably damaging 1.00
R7446:Vmn1r181 UTSW 7 23,684,356 (GRCm39) missense probably benign 0.01
R7972:Vmn1r181 UTSW 7 23,683,871 (GRCm39) missense probably benign 0.30
R8465:Vmn1r181 UTSW 7 23,684,309 (GRCm39) missense possibly damaging 0.65
R9096:Vmn1r181 UTSW 7 23,684,444 (GRCm39) missense probably benign 0.00
R9097:Vmn1r181 UTSW 7 23,684,444 (GRCm39) missense probably benign 0.00
X0067:Vmn1r181 UTSW 7 23,684,326 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- ACAAGTGTCCCGAATTTGGTG -3'
(R):5'- TGCCTCAGGAATTGGTGAG -3'

Sequencing Primer
(F):5'- ATTCCAATTAAGGTCACTGGTCCAC -3'
(R):5'- TGTGACTACCAGCATCAG -3'
Posted On 2018-04-02