Mutagenetix > Incidental Mutation

Incidental Mutation 'R6324:Gstp2'

510673

Institutional Source

Beutler Lab

Gene Symbol

Gstp2

Ensembl Gene

ENSMUSG00000038155

Gene Name

glutathione S-transferase, pi 2

Synonyms

Gst3, Gst p-2, GSTpiA, Gst-3

MMRRC Submission

044478-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.412) question?

Stock #

R6324 (G1)

Quality Score

216.009

Status

Not validated

Chromosome

Chromosomal Location

4090288-4092221 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 4090499 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 162 (I162N)

Ref Sequence

ENSEMBL: ENSMUSP00000038931 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042700] [ENSMUST00000169613]

AlphaFold

P46425

Predicted Effect

probably benign
Transcript: ENSMUST00000042700
AA Change: I162N

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000038931
Gene: ENSMUSG00000038155
AA Change: I162N

Domain	Start	End	E-Value	Type
Pfam:GST_N	2	75	4.2e-8	PFAM
Pfam:GST_C	97	188	1.4e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000169613

SMART Domains

Protein: ENSMUSP00000129565
Gene: ENSMUSG00000060803

Domain	Start	End	E-Value	Type
Pfam:GST_N	4	75	2.3e-8	PFAM
Pfam:GST_C	97	188	1.5e-15	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

96% (47/49)

MGI Phenotype

PHENOTYPE: Mutant mice with null mutations in both Gstp1 and Gstp2 exhibit an increased susceptibility to DMBA and TPA induced skin papillomas. Male mutant mice exhibit an increased body weight with age. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgef4	G	A	1: 34,762,558 (GRCm39)	A605T	unknown	Het
Atp13a3	A	G	16: 30,151,103 (GRCm39)	V1069A	possibly damaging	Het
Atp1a2	C	G	1: 172,116,903 (GRCm39)	R238P	probably damaging	Het
Baz2b	A	G	2: 59,737,292 (GRCm39)	S1877P	probably damaging	Het
Ccdc27	A	T	4: 154,120,648 (GRCm39)	S383T	probably benign	Het
Cr1l	A	G	1: 194,793,430 (GRCm39)	V377A	probably benign	Het
Dazap1	A	G	10: 80,113,494 (GRCm39)	E130G	probably benign	Het
Dchs1	G	T	7: 105,414,145 (GRCm39)	A890E	probably benign	Het
Dock10	G	A	1: 80,482,893 (GRCm39)	T2143I	probably benign	Het
Eif3j1	A	G	2: 121,871,659 (GRCm39)	D60G	probably benign	Het
Enah	A	G	1: 181,746,136 (GRCm39)	S382P	probably damaging	Het
Fam171b	A	T	2: 83,709,608 (GRCm39)	K427*	probably null	Het
Fmn2	A	T	1: 174,440,119 (GRCm39)	I1179L	possibly damaging	Het
Focad	C	T	4: 88,319,305 (GRCm39)	R1505*	probably null	Het
Frem1	T	C	4: 82,901,574 (GRCm39)	T985A	probably benign	Het
Gm3404	A	T	5: 146,464,917 (GRCm39)	Q219L	possibly damaging	Het
Gm5592	A	C	7: 40,935,959 (GRCm39)	S154R	probably damaging	Het
Gpn3	C	T	5: 122,510,638 (GRCm39)		probably benign	Het
Gpr158	A	G	2: 21,815,365 (GRCm39)	E586G	probably damaging	Het
Lin7a	T	A	10: 107,216,076 (GRCm39)		probably null	Het
Loxl4	G	A	19: 42,583,817 (GRCm39)	L745F	probably benign	Het
Ms4a19	T	A	19: 11,140,811 (GRCm39)	M3L	probably benign	Het
Mybpc1	A	G	10: 88,404,481 (GRCm39)	I172T	possibly damaging	Het
Nalcn	A	G	14: 123,647,161 (GRCm39)	W571R	possibly damaging	Het
Nkx2-5	G	C	17: 27,060,095 (GRCm39)	P79A	probably benign	Het
Nufip2	A	G	11: 77,582,487 (GRCm39)	T134A	probably benign	Het
Odad1	A	G	7: 45,591,134 (GRCm39)	E203G	probably damaging	Het
Or13p4	G	A	4: 118,547,728 (GRCm39)		probably benign	Het
Or5al1	C	T	2: 85,989,800 (GRCm39)	V305I	probably benign	Het
Or5b109	A	G	19: 13,212,468 (GRCm39)	M285V	possibly damaging	Het
Phkb	A	G	8: 86,745,171 (GRCm39)	D616G	probably benign	Het
Plch1	C	T	3: 63,688,811 (GRCm39)	W131*	probably null	Het
Prl7b1	A	T	13: 27,786,878 (GRCm39)		probably null	Het
Prop1	G	A	11: 50,843,026 (GRCm39)	P54S	probably benign	Het
Ptcd3	C	T	6: 71,862,311 (GRCm39)	V509I	probably benign	Het
Ptprg	T	A	14: 12,226,314 (GRCm38)	D527E	probably damaging	Het
Rapgef2	C	T	3: 78,986,439 (GRCm39)	V1182I	probably benign	Het
Rfx7	C	A	9: 72,525,696 (GRCm39)	P962Q	probably damaging	Het
Rsf1	CG	CGACGGCGGTG	7: 97,229,115 (GRCm39)		probably benign	Homo
Slc38a9	A	G	13: 112,862,634 (GRCm39)	I444M	probably benign	Het
Sorbs1	T	C	19: 40,310,263 (GRCm39)	T492A	probably damaging	Het
Synj1	A	T	16: 90,735,518 (GRCm39)	S1478R	probably benign	Het
Tnn	T	A	1: 159,972,774 (GRCm39)	N276I	probably damaging	Het
Trbv19	G	A	6: 41,155,692 (GRCm39)	G21D	probably damaging	Het
Ube2o	A	T	11: 116,430,185 (GRCm39)	D1184E	probably benign	Het
Vmn1r181	G	A	7: 23,684,183 (GRCm39)	R216Q	probably benign	Het
Vmn2r108	A	T	17: 20,691,977 (GRCm39)	L182*	probably null	Het
Vmn2r15	A	G	5: 109,434,137 (GRCm39)	*856R	probably null	Het
Vmn2r70	G	A	7: 85,208,087 (GRCm39)	H797Y	probably benign	Het
Zfp11	C	T	5: 129,733,587 (GRCm39)	A625T	possibly damaging	Het

Other mutations in Gstp2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02387:Gstp2	APN	19	4,091,094 (GRCm39)	unclassified	probably benign
PIT4362001:Gstp2	UTSW	19	4,090,713 (GRCm39)	missense	possibly damaging	0.82
R0196:Gstp2	UTSW	19	4,090,514 (GRCm39)	splice site	probably null
R0545:Gstp2	UTSW	19	4,091,633 (GRCm39)	missense	possibly damaging	0.88
R6478:Gstp2	UTSW	19	4,090,499 (GRCm39)	missense	probably benign	0.00
R7318:Gstp2	UTSW	19	4,091,065 (GRCm39)	missense	probably benign	0.00
R8218:Gstp2	UTSW	19	4,091,668 (GRCm39)	missense	probably benign	0.00
R9799:Gstp2	UTSW	19	4,091,901 (GRCm39)	critical splice donor site	probably null
Z1177:Gstp2	UTSW	19	4,091,583 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- AAGAAGCTCTTGTCTCTTCAGTCC -3'
(R):5'- GGCAAAGCTTTCATCGTGGG -3'

Sequencing Primer
(F):5'- AGTCCACTACTGTTTGCCATTG -3'
(R):5'- GACCAGGTGAGCATCTCTTG -3'

Posted On

2018-04-02