Mutagenetix > Incidental Mutation

Incidental Mutation 'R6336:Or13c25'

510748

Institutional Source

Beutler Lab

Gene Symbol

Or13c25

Ensembl Gene

ENSMUSG00000051593

Gene Name

olfactory receptor family 13 subfamily C member 25

Synonyms

MOR262-7, GA_x6K02T2N78B-7084885-7085844, Olfr272

MMRRC Submission

044490-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.053) question?

Stock #

R6336 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

52910833-52911792 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 52911459 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 112 (C112S)

Ref Sequence

ENSEMBL: ENSMUSP00000149339 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051600] [ENSMUST00000107667] [ENSMUST00000213989]

AlphaFold

Q8VGA0

Predicted Effect

probably damaging
Transcript: ENSMUST00000051600
AA Change: C112S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000055721
Gene: ENSMUSG00000051593
AA Change: C112S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	314	1.6e-54	PFAM
Pfam:7tm_1	41	296	9.5e-24	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000107667
AA Change: C112S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000103294
Gene: ENSMUSG00000051593
AA Change: C112S

Domain	Start	End	E-Value	Type
Pfam:7tm_1	39	294	9.5e-33	PFAM
Pfam:7tm_4	138	287	3.3e-42	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213989
AA Change: C112S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000217085

Meta Mutation Damage Score

0.1825

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.0%

Validation Efficiency

98% (45/46)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2900026A02Rik	G	T	5: 113,331,349 (GRCm39)	P955Q	possibly damaging	Het
Adgrv1	T	C	13: 81,534,100 (GRCm39)	N5827S	probably benign	Het
Akt3	T	C	1: 176,859,278 (GRCm39)	D431G	probably damaging	Het
Anapc16	A	T	10: 59,824,651 (GRCm39)	V76D	possibly damaging	Het
Ankib1	A	T	5: 3,750,377 (GRCm39)	Y755*	probably null	Het
Ankrd6	A	G	4: 32,860,411 (GRCm39)	S11P	probably damaging	Het
Card6	T	A	15: 5,128,646 (GRCm39)	K917*	probably null	Het
Ccdc152	T	C	15: 3,330,611 (GRCm39)	I21M	probably damaging	Het
Ccdc40	A	G	11: 119,122,819 (GRCm39)	E207G	possibly damaging	Het
Clca3a2	G	T	3: 144,512,239 (GRCm39)	A499E	probably benign	Het
Cntnap5b	C	T	1: 100,286,394 (GRCm39)	A383V	probably benign	Het
Ddo	T	C	10: 40,509,027 (GRCm39)	V89A	probably damaging	Het
Depdc5	T	C	5: 33,121,851 (GRCm39)	176	probably null	Het
Dlgap1	T	A	17: 71,122,284 (GRCm39)	D904E	probably damaging	Het
Ephb4	T	A	5: 137,370,347 (GRCm39)	L849H	probably damaging	Het
Fbxl13	A	G	5: 21,728,545 (GRCm39)		probably null	Het
Fer1l6	C	A	15: 58,431,081 (GRCm39)	Y245*	probably null	Het
Foxred2	A	G	15: 77,839,964 (GRCm39)	Y109H	probably damaging	Het
Gabarapl1	A	G	6: 129,514,491 (GRCm39)	D43G	probably benign	Het
Gabrb1	A	C	5: 72,187,241 (GRCm39)	E178A	possibly damaging	Het
Gbp11	A	G	5: 105,473,355 (GRCm39)			Het
Grin3b	C	A	10: 79,812,295 (GRCm39)	A845E	probably damaging	Het
Hoxd4	A	G	2: 74,557,705 (GRCm39)	Y28C	probably damaging	Het
Igsf10	G	A	3: 59,237,760 (GRCm39)	T807M	probably benign	Het
Kcnt1	A	G	2: 25,778,767 (GRCm39)		probably null	Het
Kdm5a	C	A	6: 120,375,939 (GRCm39)	T565K	probably damaging	Het
Map7d1	A	G	4: 126,130,475 (GRCm39)	S412P	probably damaging	Het
Mok	A	G	12: 110,800,558 (GRCm39)		probably null	Het
Mto1	A	T	9: 78,381,117 (GRCm39)	I73F	probably damaging	Het
Mtss2	G	A	8: 111,458,796 (GRCm39)	D310N	probably damaging	Het
Ncf2	T	C	1: 152,709,821 (GRCm39)	Y393H	probably damaging	Het
Or2f1b	T	C	6: 42,739,591 (GRCm39)	S202P	probably damaging	Het
Or2y14	G	T	11: 49,405,369 (GRCm39)	K301N	probably damaging	Het
Or4f47	A	C	2: 111,972,751 (GRCm39)	I154L	probably benign	Het
Or4k49	A	G	2: 111,494,964 (GRCm39)	H131R	possibly damaging	Het
Or52z1	T	C	7: 103,437,452 (GRCm39)	T11A	possibly damaging	Het
Phxr2	T	A	10: 98,961,952 (GRCm39)		probably benign	Het
Potefam3f	A	G	8: 20,499,951 (GRCm39)	K242E	unknown	Het
Rnf17	A	G	14: 56,658,626 (GRCm39)		probably null	Het
Serpinb9d	T	C	13: 33,378,677 (GRCm39)	M41T	probably damaging	Het
Slc12a5	T	A	2: 164,834,384 (GRCm39)		probably null	Het
Slc22a6	G	A	19: 8,599,494 (GRCm39)	R331H	probably benign	Het
Spg11	G	C	2: 121,943,440 (GRCm39)		probably null	Het
Src	T	A	2: 157,299,075 (GRCm39)	V24E	probably benign	Het
Srgap1	A	T	10: 121,761,846 (GRCm39)	Y101N	probably benign	Het
Vmn2r30	T	A	7: 7,337,307 (GRCm39)	T110S	probably benign	Het
Vwa3a	A	G	7: 120,361,646 (GRCm39)	E119G	possibly damaging	Het
Whamm	T	A	7: 81,241,512 (GRCm39)	D274E	probably damaging	Het

Other mutations in Or13c25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00159:Or13c25	APN	4	52,911,618 (GRCm39)	missense	possibly damaging	0.95
IGL02224:Or13c25	APN	4	52,911,392 (GRCm39)	missense	probably damaging	0.97
IGL03293:Or13c25	APN	4	52,910,835 (GRCm39)	makesense	probably null
K3955:Or13c25	UTSW	4	52,911,081 (GRCm39)	missense	probably damaging	1.00
R0195:Or13c25	UTSW	4	52,910,849 (GRCm39)	missense	probably benign	0.00
R0197:Or13c25	UTSW	4	52,910,849 (GRCm39)	missense	probably benign	0.00
R0445:Or13c25	UTSW	4	52,910,849 (GRCm39)	missense	probably benign	0.00
R1517:Or13c25	UTSW	4	52,911,502 (GRCm39)	nonsense	probably null
R1536:Or13c25	UTSW	4	52,911,260 (GRCm39)	missense	probably benign	0.43
R1540:Or13c25	UTSW	4	52,910,996 (GRCm39)	missense	probably benign	0.00
R1551:Or13c25	UTSW	4	52,911,397 (GRCm39)	nonsense	probably null
R1612:Or13c25	UTSW	4	52,911,501 (GRCm39)	missense	probably benign
R1920:Or13c25	UTSW	4	52,910,849 (GRCm39)	missense	probably benign
R2181:Or13c25	UTSW	4	52,911,524 (GRCm39)	missense	probably damaging	1.00
R5410:Or13c25	UTSW	4	52,910,991 (GRCm39)	missense	probably benign	0.01
R6331:Or13c25	UTSW	4	52,911,399 (GRCm39)	missense	probably damaging	1.00
R7085:Or13c25	UTSW	4	52,910,961 (GRCm39)	missense	probably benign	0.02
R7541:Or13c25	UTSW	4	52,911,376 (GRCm39)	missense	probably benign	0.04
R7727:Or13c25	UTSW	4	52,911,368 (GRCm39)	missense	possibly damaging	0.89
R7891:Or13c25	UTSW	4	52,911,663 (GRCm39)	missense	probably benign	0.01
R8782:Or13c25	UTSW	4	52,911,693 (GRCm39)	missense	probably benign	0.16
R9321:Or13c25	UTSW	4	52,911,314 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTGCAGAATGGTAATTGCACTG -3'
(R):5'- GCATGTATCTGATGATCCTGCTTG -3'

Sequencing Primer
(F):5'- TTGCACTGCAAGAGAGGTCTGTAC -3'
(R):5'- CCTGCTTGGAAATGGCGTTC -3'

Posted On

2018-04-02