Mutagenetix > Incidental Mutation

Incidental Mutation 'R6330:Vmn2r69'

510857

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r69

Ensembl Gene

ENSMUSG00000091006

Gene Name

vomeronasal 2, receptor 69

Synonyms

MMRRC Submission

044484-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R6330 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85055584-85064884 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85060835 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 250 (S250G)

Ref Sequence

ENSEMBL: ENSMUSP00000132726 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171213]

AlphaFold

G3XA45

Predicted Effect

probably benign
Transcript: ENSMUST00000171213
AA Change: S250G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000132726
Gene: ENSMUSG00000091006
AA Change: S250G

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.3e-28	PFAM
Pfam:NCD3G	507	559	1.8e-20	PFAM
Pfam:7tm_3	592	827	3.2e-54	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000207880

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

98% (46/47)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310079G19Rik	T	A	16: 88,423,939 (GRCm39)	H184L	probably benign	Het
Ccar1	A	G	10: 62,600,312 (GRCm39)	Y540H	probably damaging	Het
Celf2	A	G	2: 6,889,766 (GRCm39)	F2L	probably benign	Het
Cfap57	T	C	4: 118,426,593 (GRCm39)	I1119V	probably benign	Het
Clec4f	T	C	6: 83,629,850 (GRCm39)	Q236R	probably damaging	Het
Cstdc2	G	T	2: 148,692,745 (GRCm39)	A19D	probably damaging	Het
Cyp4a31	C	T	4: 115,421,074 (GRCm39)	P54L	probably damaging	Het
Dapk1	T	C	13: 60,909,140 (GRCm39)	V1251A	probably benign	Het
Dnah7b	G	A	1: 46,379,335 (GRCm39)	V3690M	probably damaging	Het
Dock9	A	T	14: 121,842,655 (GRCm39)	M1K	probably null	Het
Emb	T	G	13: 117,385,666 (GRCm39)		probably null	Het
Enpp5	G	A	17: 44,396,155 (GRCm39)	G356S	probably damaging	Het
F7	C	T	8: 13,085,140 (GRCm39)	H389Y	probably damaging	Het
Fam111a	T	A	19: 12,564,266 (GRCm39)	I49K	probably damaging	Het
Fam50b	T	G	13: 34,930,652 (GRCm39)	S43A	probably benign	Het
Gbp7	A	T	3: 142,252,259 (GRCm39)	Y614F	probably benign	Het
Gli3	T	A	13: 15,899,317 (GRCm39)	D901E	probably damaging	Het
Hhatl	C	T	9: 121,617,291 (GRCm39)		probably null	Het
Idua	T	A	5: 108,829,574 (GRCm39)	L432H	probably benign	Het
Ighv10-3	T	A	12: 114,487,086 (GRCm39)	T112S	possibly damaging	Het
Kcnj6	T	A	16: 94,563,460 (GRCm39)	D346V	possibly damaging	Het
Lrba	A	G	3: 86,255,664 (GRCm39)	D1002G	probably benign	Het
Lrrd1	T	A	5: 3,900,629 (GRCm39)	S311R	probably damaging	Het
Mafk	A	G	5: 139,784,948 (GRCm39)	N4S	probably benign	Het
Mfsd14b	T	C	13: 65,243,500 (GRCm39)	Y48C	probably damaging	Het
Mindy3	C	T	2: 12,361,744 (GRCm39)	A333T	probably damaging	Het
Mlxip	G	A	5: 123,533,015 (GRCm39)	C8Y	probably benign	Het
Mthfd1l	A	T	10: 3,930,234 (GRCm39)	M110L	probably benign	Het
Myo5b	G	A	18: 74,750,064 (GRCm39)	A176T	probably damaging	Het
Or14j7	T	C	17: 38,234,685 (GRCm39)	V76A	possibly damaging	Het
Or1af1	A	T	2: 37,110,136 (GRCm39)	I212F	probably benign	Het
Orc2	C	A	1: 58,539,493 (GRCm39)	V49L	probably benign	Het
Pfkp	C	T	13: 6,635,286 (GRCm39)		probably benign	Het
Pkd1l3	T	C	8: 110,373,541 (GRCm39)	F1359S	probably benign	Het
Ralgps1	A	T	2: 33,064,455 (GRCm39)	I222N	probably damaging	Het
Rrad	T	G	8: 105,356,492 (GRCm39)	E136A	probably benign	Het
Serpine3	T	C	14: 62,902,430 (GRCm39)	S14P	probably benign	Het
Slc2a12	A	G	10: 22,540,894 (GRCm39)	T250A	probably benign	Het
Slc4a5	T	G	6: 83,203,356 (GRCm39)	F85L	probably benign	Het
Stx18	T	A	5: 38,284,261 (GRCm39)		probably null	Het
Tbcd	T	C	11: 121,387,912 (GRCm39)	S348P	probably benign	Het
Usp9y	T	C	Y: 1,340,123 (GRCm39)	T1419A	probably benign	Homo
Vmn2r86	A	G	10: 130,282,396 (GRCm39)	V740A	probably benign	Het
Vwa5b2	T	A	16: 20,420,727 (GRCm39)	I846N	probably damaging	Het
Zfp74	A	T	7: 29,637,412 (GRCm39)	Y33N	probably damaging	Het
Zfp825	T	C	13: 74,628,665 (GRCm39)	T284A	possibly damaging	Het

Other mutations in Vmn2r69

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r69	APN	7	85,055,739 (GRCm39)	missense	probably benign
IGL01457:Vmn2r69	APN	7	85,055,836 (GRCm39)	missense	possibly damaging	0.87
IGL01760:Vmn2r69	APN	7	85,056,072 (GRCm39)	missense	possibly damaging	0.90
IGL01834:Vmn2r69	APN	7	85,061,576 (GRCm39)	missense	probably damaging	1.00
IGL02001:Vmn2r69	APN	7	85,056,434 (GRCm39)	missense	probably benign	0.05
IGL02057:Vmn2r69	APN	7	85,060,990 (GRCm39)	missense	possibly damaging	0.93
IGL02289:Vmn2r69	APN	7	85,056,054 (GRCm39)	missense	probably damaging	1.00
IGL02472:Vmn2r69	APN	7	85,058,960 (GRCm39)	missense	probably benign	0.01
IGL02478:Vmn2r69	APN	7	85,055,889 (GRCm39)	missense	probably damaging	1.00
IGL02554:Vmn2r69	APN	7	85,059,014 (GRCm39)	missense	probably damaging	1.00
IGL02723:Vmn2r69	APN	7	85,059,416 (GRCm39)	missense	probably damaging	1.00
R0526:Vmn2r69	UTSW	7	85,060,711 (GRCm39)	missense	probably damaging	1.00
R0560:Vmn2r69	UTSW	7	85,058,922 (GRCm39)	critical splice donor site	probably null
R0909:Vmn2r69	UTSW	7	85,055,873 (GRCm39)	missense	probably benign	0.00
R0976:Vmn2r69	UTSW	7	85,056,108 (GRCm39)	missense	probably damaging	1.00
R1158:Vmn2r69	UTSW	7	85,059,058 (GRCm39)	splice site	probably benign
R1459:Vmn2r69	UTSW	7	85,055,908 (GRCm39)	nonsense	probably null
R1482:Vmn2r69	UTSW	7	85,056,082 (GRCm39)	missense	probably damaging	1.00
R1917:Vmn2r69	UTSW	7	85,060,891 (GRCm39)	missense	probably damaging	1.00
R2016:Vmn2r69	UTSW	7	85,056,493 (GRCm39)	missense	probably damaging	0.98
R2108:Vmn2r69	UTSW	7	85,059,404 (GRCm39)	missense	probably benign
R2571:Vmn2r69	UTSW	7	85,064,764 (GRCm39)	missense	probably benign
R2910:Vmn2r69	UTSW	7	85,055,918 (GRCm39)	missense	probably damaging	1.00
R2920:Vmn2r69	UTSW	7	85,060,973 (GRCm39)	missense	probably benign	0.08
R3708:Vmn2r69	UTSW	7	85,061,029 (GRCm39)	missense	probably damaging	0.98
R3710:Vmn2r69	UTSW	7	85,055,601 (GRCm39)	missense	probably benign
R4757:Vmn2r69	UTSW	7	85,061,575 (GRCm39)	missense	probably damaging	0.99
R4823:Vmn2r69	UTSW	7	85,060,508 (GRCm39)	missense	probably benign	0.21
R4870:Vmn2r69	UTSW	7	85,060,793 (GRCm39)	missense	possibly damaging	0.93
R4918:Vmn2r69	UTSW	7	85,055,967 (GRCm39)	missense	probably benign	0.06
R5022:Vmn2r69	UTSW	7	85,060,367 (GRCm39)	missense	possibly damaging	0.72
R5174:Vmn2r69	UTSW	7	85,064,739 (GRCm39)	missense	possibly damaging	0.92
R5200:Vmn2r69	UTSW	7	85,055,717 (GRCm39)	missense	probably damaging	1.00
R5278:Vmn2r69	UTSW	7	85,060,991 (GRCm39)	missense	probably benign	0.02
R5643:Vmn2r69	UTSW	7	85,056,404 (GRCm39)	missense	probably damaging	0.98
R5996:Vmn2r69	UTSW	7	85,061,117 (GRCm39)	splice site	probably null
R6083:Vmn2r69	UTSW	7	85,055,711 (GRCm39)	missense	probably damaging	1.00
R6140:Vmn2r69	UTSW	7	85,060,657 (GRCm39)	missense	probably damaging	0.99
R6306:Vmn2r69	UTSW	7	85,064,799 (GRCm39)	missense	probably benign	0.04
R6380:Vmn2r69	UTSW	7	85,061,067 (GRCm39)	missense	probably benign
R6466:Vmn2r69	UTSW	7	85,056,378 (GRCm39)	missense	probably benign	0.01
R6542:Vmn2r69	UTSW	7	85,060,413 (GRCm39)	nonsense	probably null
R6583:Vmn2r69	UTSW	7	85,059,017 (GRCm39)	missense	probably benign
R6623:Vmn2r69	UTSW	7	85,056,309 (GRCm39)	missense	possibly damaging	0.84
R6709:Vmn2r69	UTSW	7	85,061,069 (GRCm39)	missense	probably benign	0.03
R6732:Vmn2r69	UTSW	7	85,060,351 (GRCm39)	missense	probably benign	0.00
R6741:Vmn2r69	UTSW	7	85,061,724 (GRCm39)	missense	probably benign	0.01
R7070:Vmn2r69	UTSW	7	85,060,688 (GRCm39)	missense	probably damaging	0.98
R7234:Vmn2r69	UTSW	7	85,056,315 (GRCm39)	missense	probably benign	0.22
R7323:Vmn2r69	UTSW	7	85,060,972 (GRCm39)	missense	possibly damaging	0.95
R7427:Vmn2r69	UTSW	7	85,060,467 (GRCm39)	missense	probably benign	0.28
R7428:Vmn2r69	UTSW	7	85,060,467 (GRCm39)	missense	probably benign	0.28
R7453:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7532:Vmn2r69	UTSW	7	85,059,622 (GRCm39)	missense	probably benign	0.36
R7556:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7562:Vmn2r69	UTSW	7	85,056,420 (GRCm39)	missense	probably benign
R7592:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7708:Vmn2r69	UTSW	7	85,061,755 (GRCm39)	missense	possibly damaging	0.87
R7803:Vmn2r69	UTSW	7	85,056,324 (GRCm39)	missense	probably benign	0.00
R7960:Vmn2r69	UTSW	7	85,055,973 (GRCm39)	missense	probably benign
R7966:Vmn2r69	UTSW	7	85,060,762 (GRCm39)	missense	possibly damaging	0.81
R8071:Vmn2r69	UTSW	7	85,055,713 (GRCm39)	nonsense	probably null
R8237:Vmn2r69	UTSW	7	85,060,340 (GRCm39)	missense	probably benign	0.02
R8347:Vmn2r69	UTSW	7	85,064,838 (GRCm39)	missense	probably benign	0.00
R8737:Vmn2r69	UTSW	7	85,055,783 (GRCm39)	missense	probably damaging	1.00
R8795:Vmn2r69	UTSW	7	85,064,883 (GRCm39)	start codon destroyed	probably null	0.94
R8831:Vmn2r69	UTSW	7	85,059,018 (GRCm39)	nonsense	probably null
R8856:Vmn2r69	UTSW	7	85,061,663 (GRCm39)	missense	probably benign	0.00
R8998:Vmn2r69	UTSW	7	85,060,307 (GRCm39)	missense	probably benign	0.33
R8999:Vmn2r69	UTSW	7	85,060,307 (GRCm39)	missense	probably benign	0.33
R9161:Vmn2r69	UTSW	7	85,056,177 (GRCm39)	missense	possibly damaging	0.88
R9228:Vmn2r69	UTSW	7	85,064,697 (GRCm39)	missense	probably benign	0.01
R9494:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	missense	probably damaging	1.00
R9494:Vmn2r69	UTSW	7	85,056,084 (GRCm39)	missense	probably benign	0.08
R9541:Vmn2r69	UTSW	7	85,056,209 (GRCm39)	missense	probably benign
R9620:Vmn2r69	UTSW	7	85,061,504 (GRCm39)	missense	probably benign	0.10
Z1176:Vmn2r69	UTSW	7	85,055,696 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACCAGGTATCTCAGGTTGCTG -3'
(R):5'- AGCCATGGTGTCCTTAGTTG -3'

Sequencing Primer
(F):5'- TAGAGGAGTATAGCAAGAAATCTCC -3'
(R):5'- GTTCATTTCAGATGGAACTGGATAG -3'

Posted On

2018-04-02