Mutagenetix > Incidental Mutation

Incidental Mutation 'FR4304:Apc'

511073

Institutional Source

Beutler Lab

Gene Symbol

Apc

Ensembl Gene

ENSMUSG00000005871

Gene Name

APC, WNT signaling pathway regulator

Synonyms

Min, adenomatosis polyposis coli, CC1

Accession Numbers

Essential gene?

Probably essential (E-score: 0.970) question?

Stock #

FR4304 ()

Quality Score

217.468

Status

Not validated

Chromosome

Chromosomal Location

34353977-34455605 bp(+) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

GCCAATAAA to GCCAATAAAACCAATAAA at 34415050 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000127131 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000066133] [ENSMUST00000079362] [ENSMUST00000115781] [ENSMUST00000171187]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000066133

SMART Domains

Protein: ENSMUSP00000064214
Gene: ENSMUSG00000005871

Domain	Start	End	E-Value	Type
PDB:1DEB\|B	2	55	8e-29	PDB
low complexity region	92	109	N/A	INTRINSIC
Pfam:Suppressor_APC	124	206	2.3e-32	PFAM
low complexity region	211	222	N/A	INTRINSIC
low complexity region	234	247	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000079362

SMART Domains

Protein: ENSMUSP00000078337
Gene: ENSMUSG00000005871

Domain	Start	End	E-Value	Type
Pfam:APC_N_CC	4	55	6e-32	PFAM
low complexity region	92	109	N/A	INTRINSIC
Pfam:Suppressor_APC	125	205	2e-24	PFAM
low complexity region	211	222	N/A	INTRINSIC
low complexity region	234	247	N/A	INTRINSIC
ARM	338	390	6.14e-5	SMART
ARM	457	508	1.62e-4	SMART
ARM	510	551	8.56e-4	SMART
ARM	554	595	4.45e-2	SMART
ARM	597	642	5.76e1	SMART
ARM	647	687	1.29e-7	SMART
Pfam:Arm_APC_u3	730	1017	5e-170	PFAM
Pfam:APC_15aa	1018	1032	1.1e-8	PFAM
Pfam:APC_u5	1034	1133	7.6e-55	PFAM
Pfam:APC_15aa	1154	1168	1.6e-8	PFAM
Pfam:APC_15aa	1171	1185	1.9e-9	PFAM
low complexity region	1187	1204	N/A	INTRINSIC
Pfam:APC_crr	1255	1279	1.5e-15	PFAM
Pfam:APC_u9	1280	1367	1.9e-34	PFAM
Pfam:APC_crr	1370	1393	2.2e-10	PFAM
low complexity region	1431	1449	N/A	INTRINSIC
Pfam:APC_crr	1485	1509	2.1e-9	PFAM
low complexity region	1532	1548	N/A	INTRINSIC
Pfam:SAMP	1568	1587	2.7e-11	PFAM
Pfam:APC_crr	1635	1659	1.9e-15	PFAM
Pfam:APC_u13	1660	1716	1.3e-31	PFAM
Pfam:SAMP	1717	1736	3.2e-12	PFAM
Pfam:APC_u14	1737	1837	1e-46	PFAM
Pfam:APC_crr	1839	1864	6.8e-15	PFAM
Pfam:APC_u15	1865	1945	1.8e-40	PFAM
Pfam:APC_crr	1947	1971	1.6e-14	PFAM
Pfam:APC_crr	2007	2030	1.8e-14	PFAM
Pfam:SAMP	2033	2052	1.6e-13	PFAM
low complexity region	2112	2146	N/A	INTRINSIC
Pfam:APC_basic	2223	2579	1.5e-110	PFAM
low complexity region	2626	2638	N/A	INTRINSIC
Pfam:EB1_binding	2670	2842	9.3e-89	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000115781

SMART Domains

Protein: ENSMUSP00000111447
Gene: ENSMUSG00000005871

Domain	Start	End	E-Value	Type
PDB:1DEB\|B	2	55	1e-27	PDB
low complexity region	92	109	N/A	INTRINSIC
Pfam:Suppressor_APC	124	206	1.5e-31	PFAM
low complexity region	211	222	N/A	INTRINSIC
ARM	304	356	6.14e-5	SMART
ARM	423	474	1.62e-4	SMART
ARM	476	517	8.56e-4	SMART
ARM	520	561	4.45e-2	SMART
ARM	563	608	5.76e1	SMART
ARM	613	653	1.29e-7	SMART
Pfam:Arm	655	695	1.7e-6	PFAM
low complexity region	797	810	N/A	INTRINSIC
low complexity region	880	892	N/A	INTRINSIC
low complexity region	923	935	N/A	INTRINSIC
Pfam:APC_15aa	984	999	3.7e-9	PFAM
Pfam:APC_15aa	1100	1115	8.4e-8	PFAM
Pfam:APC_15aa	1120	1135	9.9e-9	PFAM
Pfam:APC_15aa	1137	1152	1.2e-9	PFAM
low complexity region	1153	1170	N/A	INTRINSIC
Pfam:APC_crr	1220	1245	7.5e-15	PFAM
low complexity region	1320	1331	N/A	INTRINSIC
Pfam:APC_crr	1334	1359	2.8e-11	PFAM
low complexity region	1397	1415	N/A	INTRINSIC
Pfam:APC_crr	1450	1475	2.2e-8	PFAM
low complexity region	1498	1514	N/A	INTRINSIC
Pfam:SAMP	1533	1553	8.4e-12	PFAM
Pfam:APC_crr	1600	1625	3.5e-13	PFAM
Pfam:SAMP	1682	1702	5e-12	PFAM
low complexity region	1732	1744	N/A	INTRINSIC
Pfam:APC_crr	1805	1830	3.1e-12	PFAM
low complexity region	1866	1877	N/A	INTRINSIC
Pfam:APC_crr	1912	1937	3.5e-13	PFAM
Pfam:APC_crr	1971	1996	7.1e-14	PFAM
Pfam:SAMP	1999	2018	4.6e-13	PFAM
low complexity region	2078	2112	N/A	INTRINSIC
Pfam:APC_basic	2189	2545	1.1e-131	PFAM
low complexity region	2592	2604	N/A	INTRINSIC
Pfam:EB1_binding	2636	2808	2.9e-90	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000170023

Predicted Effect

probably benign
Transcript: ENSMUST00000171187

SMART Domains

Protein: ENSMUSP00000127131
Gene: ENSMUSG00000005871

Domain	Start	End	E-Value	Type
low complexity region	9	20	N/A	INTRINSIC
low complexity region	23	52	N/A	INTRINSIC
low complexity region	102	119	N/A	INTRINSIC
Pfam:Suppressor_APC	134	216	5.2e-32	PFAM
ARM	320	372	6.14e-5	SMART
ARM	439	490	1.62e-4	SMART
ARM	492	533	8.56e-4	SMART
ARM	536	577	4.45e-2	SMART
ARM	579	624	5.76e1	SMART
ARM	629	669	1.29e-7	SMART
Pfam:Arm	671	711	6.3e-7	PFAM
low complexity region	813	826	N/A	INTRINSIC
low complexity region	896	908	N/A	INTRINSIC
low complexity region	939	951	N/A	INTRINSIC
Pfam:APC_15aa	1000	1015	1.4e-9	PFAM
Pfam:APC_15aa	1116	1131	3.1e-8	PFAM

Coding Region Coverage

1x: 99.8%
3x: 99.6%
10x: 98.6%
20x: 96.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
PHENOTYPE: Most targeted and hypomorphic heterozygous mutants develop intestinal polyps and colorectal cancer, associated with anemia from intestinal bleeding. Homozygotes are embryonic lethal. Homozygotes for a mild alleles survive and have less extreme tumor incidence. [provided by MGI curators]

Allele List at MGI

All alleles(88) : Targeted(25) Gene trapped(62) Chemically induced(1)

Other mutations in this stock

Total: 137 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001K19Rik	CTT	CTTTTT	12: 110,634,883 (GRCm39)		probably benign	Homo
1700001K19Rik	TTC	TTCGTC	12: 110,634,884 (GRCm39)		probably benign	Homo
4930433I11Rik	ACCTC	AC	7: 40,642,480 (GRCm39)		probably benign	Het
4930447C04Rik	AAGT	A	12: 72,928,061 (GRCm39)		probably benign	Homo
Acbd4	CAG	CAGACTAG	11: 102,994,931 (GRCm39)		probably null	Homo
Ahdc1	CT	CTCTT	4: 132,790,070 (GRCm39)		probably benign	Homo
Alpk3	TCT	TCTGCT	7: 80,727,510 (GRCm39)		probably benign	Het
Anapc4	C	T	5: 53,021,868 (GRCm39)	T650M	probably damaging	Homo
Ankhd1	GGCGGC	GGCGGCTGCGGC	18: 36,693,977 (GRCm39)		probably benign	Het
Ankrd35	TAGC	TAGCAGC	3: 96,591,163 (GRCm39)		probably benign	Homo
Apol6	TTGT	TTGTCTGT	15: 76,935,636 (GRCm39)		probably null	Het
Arhgap30	TGGCCC	TGGCCCTGGCCCAGGCCTTGGCCCCGGCCC	1: 171,232,736 (GRCm39)		probably benign	Het
Arpc1b	GCC	GCCTGTCC	5: 145,063,601 (GRCm39)		probably null	Het
Blm	CT	CTACGT	7: 80,113,521 (GRCm39)		probably null	Homo
Blm	TCCTCCTCCTCC	TCCTCCTCCTCCACCTCCTCCTCC	7: 80,162,667 (GRCm39)		probably benign	Het
Btnl10	GA	GAATA	11: 58,814,756 (GRCm39)		probably benign	Homo
Cacna1f	AGG	AGGCGG	X: 7,486,300 (GRCm39)		probably benign	Het
Calhm3	CG	CGG	19: 47,140,335 (GRCm39)		probably null	Homo
Catsper2	C	CTTTTACTTTTTA	2: 121,228,023 (GRCm39)		probably null	Homo
Catsper2	CAT	CATTAT	2: 121,228,263 (GRCm39)		probably benign	Het
Ccdc121	GAGAAG	GAG	5: 31,644,717 (GRCm39)		probably benign	Homo
Ccdc15	AC	ACTTTCC	9: 37,226,453 (GRCm39)		probably null	Het
Ccdc162	T	C	10: 41,432,117 (GRCm39)	D1792G	possibly damaging	Het
Ccdc170	CCA	CCATCA	10: 4,511,021 (GRCm39)		probably benign	Het
Ccdc73	TAAG	T	2: 104,822,185 (GRCm39)		probably benign	Homo
Ccdc85c	GCC	GCCCCC	12: 108,240,871 (GRCm39)		probably benign	Het
Cd22	C	T	7: 30,577,507 (GRCm39)	R2H	possibly damaging	Het
Cd80	AGA	AGAGGA	16: 38,306,677 (GRCm39)		probably benign	Homo
Cep89	GACT	G	7: 35,109,066 (GRCm39)		probably benign	Het
Cfap74	A	G	4: 155,500,217 (GRCm39)	D21G	possibly damaging	Homo
Cgref1	T	TCTA	5: 31,091,124 (GRCm39)		probably benign	Homo
Chd4	GCC	GCCACTCCC	6: 125,099,107 (GRCm39)		probably benign	Het
Cnpy3	TCC	TCCCCC	17: 47,047,669 (GRCm39)		probably benign	Het
Cnpy3	TCC	TCCACC	17: 47,047,672 (GRCm39)		probably benign	Het
Cntnap1	AGCCCC	AGCCCCCGCCCC	11: 101,080,407 (GRCm39)		probably benign	Het
Cntnap1	CCCCAG	CCCCAGACCCAG	11: 101,080,415 (GRCm39)		probably benign	Het
Col2a1	C	A	15: 97,886,862 (GRCm39)		probably null	Homo
Cpeb4	T	TGA	11: 31,877,638 (GRCm39)		probably benign	Homo
Cpne1	AGA	AGAGAGA	2: 155,913,945 (GRCm39)		probably null	Homo
Cttnbp2	ATTGCTG	ATTGCTGTTGCTG	6: 18,367,457 (GRCm39)		probably benign	Het
Dhx37	CTGG	C	5: 125,504,594 (GRCm39)		probably benign	Het
Dhx8	CGAGAC	CGAGACGGAGAC	11: 101,629,014 (GRCm39)		probably benign	Homo
Dnaaf9	TCC	TCCCCC	2: 130,612,668 (GRCm39)		probably benign	Het
Dnah12	G	T	14: 26,571,342 (GRCm39)	G2817V	probably damaging	Homo
Dst	C	A	1: 34,240,045 (GRCm39)	S1798Y	probably damaging	Het
Eif3a	TA	TATTTCA	19: 60,763,728 (GRCm39)		probably benign	Homo
Ermn	TTC	TTCCTC	2: 57,938,090 (GRCm39)		probably benign	Het
Ermn	CTT	CTTGTT	2: 57,938,098 (GRCm39)		probably benign	Het
Fbxo43	TGTGCC	TGTGCCAGTGCC	15: 36,152,243 (GRCm39)		probably benign	Het
Fbxo43	GCCTGT	GCCTGTCCCTGT	15: 36,152,246 (GRCm39)		probably benign	Het
Fbxo43	GCCTGT	GCCTGTTCCTGT	15: 36,152,240 (GRCm39)		probably benign	Het
Fmn1	TCC	TCCTCCACC	2: 113,356,128 (GRCm39)		probably benign	Homo
Fmn1	TCCTCC	TCCTCCCCCTCC	2: 113,356,119 (GRCm39)		probably benign	Het
Foxd3	GGACCCTACGGCCG	GG	4: 99,545,633 (GRCm39)		probably benign	Homo
Frmpd2	G	T	14: 33,232,978 (GRCm39)	L399F	probably damaging	Homo
Gabre	ACTCCGGCTCCGGCTCAGGCTCAGGCTCCGGCTCAGGCTCCGGCTCCGGCTCCGGCTC	ACTCCGGCTCCGGCTCCGGCTCAGGCTCAGGCTCCGGCTCAGGCTCCGGCTCCGGCTCCGGCTC	X: 71,313,648 (GRCm39)		probably benign	Homo
Gbp2b	A	G	3: 142,309,413 (GRCm39)	I175V	probably benign	Het
Gm4340	CAG	CAGAAG	10: 104,031,933 (GRCm39)		probably benign	Het
Gm4340	AGC	AGCGGC	10: 104,031,943 (GRCm39)		probably benign	Het
Gm5114	T	C	7: 39,060,529 (GRCm39)	R107G	probably benign	Het
Gm5114	A	C	7: 39,060,530 (GRCm39)	H106Q	probably benign	Het
H1f6	GAGAA	GA	13: 23,879,903 (GRCm39)		probably benign	Homo
H2-Q4	G	A	17: 35,599,381 (GRCm39)	D155N	probably damaging	Het
H2-T10	TGTTTCCCACTG	T	17: 36,431,173 (GRCm39)		probably null	Het
Ifi203	C	T	1: 173,755,894 (GRCm39)		probably benign	Het
Ifi208	ATGGTG	ATG	1: 173,505,264 (GRCm39)		probably benign	Homo
Ighv5-9	C	T	12: 113,625,497 (GRCm39)	S82N	probably benign	Homo
Il17rd	CGG	CGGTGG	14: 26,804,637 (GRCm39)		probably benign	Het
Il2	AGTGG	AGTGGGGCTTGAGGTGG	3: 37,179,975 (GRCm39)		probably benign	Het
Ipo9	TCC	TCCGCC	1: 135,314,013 (GRCm39)		probably benign	Het
Ipo9	CCT	CCTACT	1: 135,314,017 (GRCm39)		probably null	Het
Isg20l2	AAG	AAGCAG	3: 87,839,019 (GRCm39)		probably benign	Homo
Kmt2b	TCCTCC	TCCTCCCCCTCC	7: 30,285,788 (GRCm39)		probably benign	Het
Kmt2c	TGCTGCTG	TGCTGCTGCTGCTG	5: 25,520,764 (GRCm39)		probably benign	Homo
Krt10	CGCC	CGCCGCC	11: 99,277,025 (GRCm39)		probably benign	Het
Krt10	CCTCCT	CCTCCTACTCCT	11: 99,280,100 (GRCm39)		probably benign	Het
Las1l	GAG	GAGCAG	X: 94,984,426 (GRCm39)		probably benign	Het
Las1l	AGG	AGGCGG	X: 94,984,427 (GRCm39)		probably benign	Het
Lkaaear1	GCTCCAGCTCCAGCTCCAGCTCCA	GCTCCAGCTCCATCTCCAGCTCCAGCTCCAGCTCCA	2: 181,339,372 (GRCm39)		probably benign	Het
Lrch1	A	T	14: 75,057,005 (GRCm39)	C241S	possibly damaging	Het
Lrit3	G	GCTT	3: 129,582,468 (GRCm39)		probably benign	Het
Maml2	GCAGCAGCAACAGCAGCA	GCAGCAGCA	9: 13,532,755 (GRCm39)		probably benign	Homo
Mast4	T	TTTC	13: 102,871,370 (GRCm39)		probably benign	Het
Med12l	AGC	AGCGGC	3: 59,183,403 (GRCm39)		probably benign	Het
Muc21	T	G	17: 35,933,013 (GRCm39)		probably benign	Homo
Noc2l	TGC	TGCAGC	4: 156,324,553 (GRCm39)		probably benign	Het
Nrg3	G	GACATTT	14: 38,119,230 (GRCm39)		probably benign	Homo
Or51q1	TCC	TCCC	7: 103,629,110 (GRCm39)		probably null	Het
Padi3	TCTCAC	TC	4: 140,520,283 (GRCm39)		probably benign	Homo
Patl2	GCT	GCTTCT	2: 121,956,616 (GRCm39)		probably benign	Het
Pdik1l	TTTT	TTTTGTTTTTGGTTT	4: 134,006,685 (GRCm39)		probably null	Homo
Pik3c2g	AG	AGAGGG	6: 139,612,654 (GRCm39)		probably null	Homo
Plekhs1	T	TTCAGACCTCCCC	19: 56,468,290 (GRCm39)		probably benign	Het
Prkd3	G	T	17: 79,283,249 (GRCm39)		probably null	Homo
Prkn	G	A	17: 12,073,650 (GRCm39)	V323M	probably damaging	Het
Prr13	TCC	TCCCCC	15: 102,370,612 (GRCm39)		probably benign	Homo
Prrc2b	G	A	2: 32,111,179 (GRCm39)	A1852T	probably damaging	Homo
Ptms	CTT	CTTTTT	6: 124,891,421 (GRCm39)		probably benign	Homo
Rtl1	TTCCTCTTCCTCCTC	TTCCTC	12: 109,557,632 (GRCm39)		probably benign	Homo
Scaf4	TGCGGC	TGC	16: 90,026,742 (GRCm39)		probably benign	Homo
Serac1	T	A	17: 6,121,083 (GRCm39)	K70N	probably damaging	Homo
Six3	CGG	CGGTGG	17: 85,928,796 (GRCm39)		probably benign	Het
Sry	GTG	GTGCTG	Y: 2,662,837 (GRCm39)		probably benign	Homo
Stard8	GGAAGA	GGAAGAAGA	X: 98,110,111 (GRCm39)		probably benign	Het
Supt20	TTCAGCA	TTCAGCATCAGCA	3: 54,635,068 (GRCm39)		probably benign	Het
Supt20	CAGCAG	CAGCAGTAGCAG	3: 54,635,085 (GRCm39)		probably null	Het
Supt20	AGCAGC	AGCAGCGGCAGC	3: 54,635,083 (GRCm39)		probably benign	Het
Sytl1	CTCT	C	4: 132,984,304 (GRCm39)		probably benign	Homo
Tcof1	AGC	AGCGGC	18: 60,968,814 (GRCm39)		probably benign	Het
Tdpoz2	T	TCA	3: 93,558,922 (GRCm39)		probably null	Homo
Tert	GCC	GCCAAGGGTTCC	13: 73,796,421 (GRCm39)		probably benign	Homo
Tfeb	GCA	GCAACA	17: 48,097,019 (GRCm39)		probably benign	Het
Ticrr	ATT	ATTTTT	7: 79,344,059 (GRCm39)		probably benign	Homo
Tnfaip8	ACACACACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	AC	18: 50,179,906 (GRCm39)		probably null	Homo
Tnfrsf9	T	TGCC	4: 151,018,852 (GRCm39)		probably benign	Homo
Tob1	GCA	GCAACA	11: 94,105,290 (GRCm39)		probably benign	Het
Tob1	CA	CAGTA	11: 94,105,303 (GRCm39)		probably null	Het
Trav15-2-dv6-2	GGGAG	GGGAGGAG	14: 53,887,207 (GRCm39)		probably benign	Homo
Triobp	TCGTCG	TCGTCGTCG	15: 78,877,587 (GRCm39)		probably benign	Homo
Tsbp1	A	AGCC	17: 34,679,029 (GRCm39)		probably benign	Het
Tsbp1	GC	GCATC	17: 34,679,051 (GRCm39)		probably benign	Het
Tsen2	AGG	AGGGGG	6: 115,537,030 (GRCm39)		probably benign	Het
Ubtf	TCC	TCCGCC	11: 102,197,782 (GRCm39)		probably benign	Het
Ubtf	CTCGTCGTC	CTCGTCGTCGTC	11: 102,197,784 (GRCm39)		probably benign	Het
Vars1	TGG	TGGAGTCCTGGGCGG	17: 35,234,965 (GRCm39)		probably benign	Homo
Vmn1r171	C	T	7: 23,332,105 (GRCm39)	A110V	probably benign	Het
Vmn2r31	G	T	7: 7,387,607 (GRCm39)	Q655K	probably damaging	Het
Vmn2r87	C	T	10: 130,314,583 (GRCm39)	M334I	probably benign	Homo
Zc3h13	CG	CGAGATGTGTG	14: 75,561,050 (GRCm39)		probably benign	Het
Zc3h13	AGATGTGCG	AGATGTGCGGGATGTGCG	14: 75,561,043 (GRCm39)		probably benign	Het
Zfp282	GGC	GGCCGC	6: 47,881,731 (GRCm39)		probably benign	Het
Zfp384	AGGC	AGGCCCAGGCCCCGGC	6: 125,013,456 (GRCm39)		probably benign	Het
Zfp459	TGA	TGAGCGA	13: 67,556,393 (GRCm39)		probably null	Homo
Zfp462	GCCACC	GCCACCTCAGCCACAACCACC	4: 55,009,757 (GRCm39)		probably benign	Het
Zfp462	CCACC	CCACCTCAGCCACAGTCACC	4: 55,009,758 (GRCm39)		probably benign	Het
Zfp598	CACCAC	CACCACAACCAC	17: 24,899,749 (GRCm39)		probably benign	Het
Zfp831	CCT	CCTGCT	2: 174,487,274 (GRCm39)		probably benign	Het

Other mutations in Apc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00507:Apc	APN	18	34,449,979 (GRCm39)	missense	probably benign	0.01
IGL00898:Apc	APN	18	34,450,147 (GRCm39)	missense	probably damaging	1.00
IGL01111:Apc	APN	18	34,448,189 (GRCm39)	missense	possibly damaging	0.95
IGL01347:Apc	APN	18	34,450,723 (GRCm39)	missense	probably damaging	1.00
IGL01375:Apc	APN	18	34,446,707 (GRCm39)	missense	probably damaging	1.00
IGL01805:Apc	APN	18	34,451,271 (GRCm39)	missense	probably benign	0.02
IGL01997:Apc	APN	18	34,448,476 (GRCm39)	missense	probably benign	0.00
IGL02033:Apc	APN	18	34,443,772 (GRCm39)	missense	probably damaging	1.00
IGL02323:Apc	APN	18	34,448,863 (GRCm39)	nonsense	probably null
IGL02373:Apc	APN	18	34,449,212 (GRCm39)	missense	probably damaging	1.00
IGL02379:Apc	APN	18	34,431,798 (GRCm39)	missense	probably benign	0.45
IGL02456:Apc	APN	18	34,446,935 (GRCm39)	nonsense	probably null
IGL02552:Apc	APN	18	34,446,035 (GRCm39)	missense	possibly damaging	0.90
IGL02676:Apc	APN	18	34,448,687 (GRCm39)	missense	probably damaging	1.00
IGL02756:Apc	APN	18	34,447,588 (GRCm39)	missense	probably damaging	1.00
IGL02938:Apc	APN	18	34,448,281 (GRCm39)	missense	probably damaging	0.98
IGL02974:Apc	APN	18	34,401,436 (GRCm39)	splice site	probably benign
IGL03124:Apc	APN	18	34,433,038 (GRCm39)	missense	probably damaging	0.98
IGL03201:Apc	APN	18	34,445,429 (GRCm39)	missense	probably damaging	1.00
IGL03339:Apc	APN	18	34,431,527 (GRCm39)	missense	probably damaging	1.00
FR4342:Apc	UTSW	18	34,415,052 (GRCm39)	intron	probably benign
FR4449:Apc	UTSW	18	34,415,058 (GRCm39)	intron	probably benign
FR4449:Apc	UTSW	18	34,415,053 (GRCm39)	intron	probably benign
FR4548:Apc	UTSW	18	34,415,051 (GRCm39)	intron	probably benign
FR4737:Apc	UTSW	18	34,415,052 (GRCm39)	intron	probably benign
FR4976:Apc	UTSW	18	34,415,057 (GRCm39)	nonsense	probably null
FR4976:Apc	UTSW	18	34,415,053 (GRCm39)	intron	probably benign
FR4976:Apc	UTSW	18	34,415,051 (GRCm39)	intron	probably benign
R0385:Apc	UTSW	18	34,448,997 (GRCm39)	missense	probably damaging	1.00
R0535:Apc	UTSW	18	34,394,125 (GRCm39)	missense	probably damaging	1.00
R0561:Apc	UTSW	18	34,446,356 (GRCm39)	missense	possibly damaging	0.94
R0590:Apc	UTSW	18	34,449,283 (GRCm39)	nonsense	probably null
R0626:Apc	UTSW	18	34,451,507 (GRCm39)	missense	probably damaging	1.00
R0991:Apc	UTSW	18	34,449,160 (GRCm39)	missense	probably damaging	1.00
R1564:Apc	UTSW	18	34,448,202 (GRCm39)	missense	probably benign	0.00
R1663:Apc	UTSW	18	34,401,378 (GRCm39)	missense	probably damaging	0.98
R1737:Apc	UTSW	18	34,450,075 (GRCm39)	missense	probably damaging	1.00
R1739:Apc	UTSW	18	34,445,371 (GRCm39)	missense	probably damaging	1.00
R1835:Apc	UTSW	18	34,450,130 (GRCm39)	missense	probably damaging	1.00
R1887:Apc	UTSW	18	34,405,521 (GRCm39)	missense	probably damaging	1.00
R1957:Apc	UTSW	18	34,450,388 (GRCm39)	missense	probably damaging	1.00
R1974:Apc	UTSW	18	34,433,057 (GRCm39)	missense	possibly damaging	0.62
R2005:Apc	UTSW	18	34,443,962 (GRCm39)	critical splice donor site	probably null
R2013:Apc	UTSW	18	34,448,644 (GRCm39)	missense	probably damaging	0.98
R2014:Apc	UTSW	18	34,448,644 (GRCm39)	missense	probably damaging	0.98
R2015:Apc	UTSW	18	34,448,644 (GRCm39)	missense	probably damaging	0.98
R2017:Apc	UTSW	18	34,446,655 (GRCm39)	missense	probably benign	0.00
R2056:Apc	UTSW	18	34,449,481 (GRCm39)	missense	probably damaging	1.00
R2108:Apc	UTSW	18	34,402,282 (GRCm39)	missense	probably damaging	1.00
R2120:Apc	UTSW	18	34,409,654 (GRCm39)	missense	probably damaging	1.00
R2131:Apc	UTSW	18	34,445,098 (GRCm39)	missense	possibly damaging	0.51
R2133:Apc	UTSW	18	34,445,098 (GRCm39)	missense	possibly damaging	0.51
R2291:Apc	UTSW	18	34,445,544 (GRCm39)	missense	probably benign	0.45
R2332:Apc	UTSW	18	34,450,112 (GRCm39)	missense	possibly damaging	0.50
R2360:Apc	UTSW	18	34,394,179 (GRCm39)	missense	probably damaging	1.00
R2407:Apc	UTSW	18	34,447,315 (GRCm39)	missense	possibly damaging	0.77
R2507:Apc	UTSW	18	34,449,590 (GRCm39)	missense	possibly damaging	0.77
R2940:Apc	UTSW	18	34,409,723 (GRCm39)	missense	probably damaging	1.00
R3404:Apc	UTSW	18	34,446,655 (GRCm39)	missense	probably benign	0.00
R3411:Apc	UTSW	18	34,402,312 (GRCm39)	splice site	probably benign
R3778:Apc	UTSW	18	34,446,134 (GRCm39)	missense	probably damaging	1.00
R3826:Apc	UTSW	18	34,412,388 (GRCm39)	missense	possibly damaging	0.93
R4599:Apc	UTSW	18	34,451,040 (GRCm39)	nonsense	probably null
R4611:Apc	UTSW	18	34,451,618 (GRCm39)	missense	probably damaging	1.00
R4664:Apc	UTSW	18	34,431,647 (GRCm39)	missense	probably damaging	0.98
R4969:Apc	UTSW	18	34,445,971 (GRCm39)	nonsense	probably null
R5007:Apc	UTSW	18	34,446,016 (GRCm39)	missense	probably damaging	1.00
R5066:Apc	UTSW	18	34,449,158 (GRCm39)	missense	probably damaging	1.00
R5112:Apc	UTSW	18	34,449,162 (GRCm39)	nonsense	probably null
R5259:Apc	UTSW	18	34,447,343 (GRCm39)	missense	probably benign	0.29
R5440:Apc	UTSW	18	34,354,213 (GRCm39)	unclassified	probably benign
R5508:Apc	UTSW	18	34,431,633 (GRCm39)	missense	probably damaging	0.97
R5512:Apc	UTSW	18	34,443,962 (GRCm39)	critical splice donor site	probably benign
R5850:Apc	UTSW	18	34,451,116 (GRCm39)	missense	possibly damaging	0.94
R5951:Apc	UTSW	18	34,450,199 (GRCm39)	missense	possibly damaging	0.89
R5966:Apc	UTSW	18	34,354,140 (GRCm39)	utr 5 prime	probably benign
R6081:Apc	UTSW	18	34,423,164 (GRCm39)	missense	possibly damaging	0.93
R6116:Apc	UTSW	18	34,449,508 (GRCm39)	missense	probably damaging	1.00
R6351:Apc	UTSW	18	34,445,265 (GRCm39)	missense	probably damaging	1.00
R6354:Apc	UTSW	18	34,445,581 (GRCm39)	missense	probably benign	0.02
R6467:Apc	UTSW	18	34,402,252 (GRCm39)	missense	probably benign	0.22
R6974:Apc	UTSW	18	34,431,480 (GRCm39)	missense	possibly damaging	0.65
R7027:Apc	UTSW	18	34,445,129 (GRCm39)	missense	probably damaging	1.00
R7096:Apc	UTSW	18	34,449,010 (GRCm39)	missense	probably damaging	1.00
R7289:Apc	UTSW	18	34,448,324 (GRCm39)	missense	probably damaging	1.00
R7439:Apc	UTSW	18	34,445,126 (GRCm39)	missense	probably damaging	1.00
R7441:Apc	UTSW	18	34,445,126 (GRCm39)	missense	probably damaging	1.00
R7534:Apc	UTSW	18	34,450,015 (GRCm39)	missense	probably damaging	1.00
R7685:Apc	UTSW	18	34,447,261 (GRCm39)	missense	probably damaging	1.00
R7814:Apc	UTSW	18	34,405,592 (GRCm39)	missense	probably damaging	0.98
R7954:Apc	UTSW	18	34,447,321 (GRCm39)	missense	probably damaging	0.99
R8352:Apc	UTSW	18	34,445,804 (GRCm39)	missense	possibly damaging	0.54
R8452:Apc	UTSW	18	34,445,804 (GRCm39)	missense	possibly damaging	0.54
R8497:Apc	UTSW	18	34,446,083 (GRCm39)	missense	possibly damaging	0.81
R8545:Apc	UTSW	18	34,450,084 (GRCm39)	missense	possibly damaging	0.94
R8554:Apc	UTSW	18	34,445,999 (GRCm39)	missense	probably damaging	1.00
R8955:Apc	UTSW	18	34,401,370 (GRCm39)	missense	probably damaging	1.00
R9014:Apc	UTSW	18	34,354,074 (GRCm39)	start gained	probably benign
R9061:Apc	UTSW	18	34,446,251 (GRCm39)	missense	probably damaging	1.00
R9147:Apc	UTSW	18	34,450,710 (GRCm39)	missense	probably damaging	1.00
R9318:Apc	UTSW	18	34,447,040 (GRCm39)	missense	possibly damaging	0.69
R9521:Apc	UTSW	18	34,445,738 (GRCm39)	missense	probably benign	0.24
R9546:Apc	UTSW	18	34,445,311 (GRCm39)	missense	possibly damaging	0.86
R9547:Apc	UTSW	18	34,445,311 (GRCm39)	missense	possibly damaging	0.86
R9557:Apc	UTSW	18	34,451,412 (GRCm39)	missense	probably damaging	1.00
R9592:Apc	UTSW	18	34,443,823 (GRCm39)	nonsense	probably null
R9675:Apc	UTSW	18	34,449,247 (GRCm39)	missense	probably damaging	1.00
R9736:Apc	UTSW	18	34,450,823 (GRCm39)	missense	probably damaging	1.00
R9792:Apc	UTSW	18	34,447,628 (GRCm39)	missense	probably damaging	1.00
R9793:Apc	UTSW	18	34,447,628 (GRCm39)	missense	probably damaging	1.00
R9795:Apc	UTSW	18	34,447,628 (GRCm39)	missense	probably damaging	1.00
RF046:Apc	UTSW	18	34,415,062 (GRCm39)	critical splice donor site	probably benign
RF063:Apc	UTSW	18	34,415,062 (GRCm39)	critical splice donor site	probably benign
X0021:Apc	UTSW	18	34,445,161 (GRCm39)	missense	probably damaging	1.00
X0025:Apc	UTSW	18	34,445,429 (GRCm39)	missense	probably damaging	1.00
Z1088:Apc	UTSW	18	34,446,220 (GRCm39)	nonsense	probably null
Z1177:Apc	UTSW	18	34,447,516 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- CCTGTTACAGTCATTCACTCAGGAG -3'
(R):5'- GGTAACTGAACTGAATTGTGGG -3'

Sequencing Primer
(F):5'- TACTCAGCAAATGCAGGTGTC -3'
(R):5'- CTGAACTGAATTGTGGGACAAAG -3'

Posted On

2018-04-05