Mutagenetix > Incidental Mutation

Incidental Mutation 'FR4589:Bcas3'

511387

Institutional Source

Beutler Lab

Gene Symbol

Bcas3

Ensembl Gene

ENSMUSG00000059439

Gene Name

BCAS3 microtubule associated cell migration factor

Synonyms

rudhira, 1500019F07Rik, Phaf2, breast carcinoma amplified sequence 3, K20D4, 2610028P08Rik

Accession Numbers

Essential gene?

Probably essential (E-score: 0.768) question?

Stock #

FR4589 ()

Quality Score

221.999

Status

Not validated

Chromosome

Chromosomal Location

85243993-85716884 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 85400323 bp (GRCm39)

Zygosity

Homozygous

Amino Acid Change

Valine to Isoleucine at position 431 (V431I)

Ref Sequence

ENSEMBL: ENSMUSP00000103697 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074875] [ENSMUST00000092821] [ENSMUST00000092822] [ENSMUST00000108056] [ENSMUST00000108061] [ENSMUST00000108062]

AlphaFold

Q8CCN5

Predicted Effect

probably benign
Transcript: ENSMUST00000074875
AA Change: V431I

PolyPhen 2 Score 0.076 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000074416
Gene: ENSMUSG00000059439
AA Change: V431I

Domain	Start	End	E-Value	Type
Blast:WD40	56	104	3e-17	BLAST
WD40	340	380	7.7e-1	SMART
WD40	390	433	2.47e1	SMART
low complexity region	480	494	N/A	INTRINSIC
low complexity region	505	514	N/A	INTRINSIC
Pfam:BCAS3	521	792	2.3e-33	PFAM
low complexity region	885	901	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000092821
AA Change: V431I

PolyPhen 2 Score 0.036 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000090496
Gene: ENSMUSG00000059439
AA Change: V431I

Domain	Start	End	E-Value	Type
Blast:WD40	56	104	3e-17	BLAST
WD40	340	380	7.7e-1	SMART
WD40	390	433	2.47e1	SMART
low complexity region	480	494	N/A	INTRINSIC
low complexity region	505	514	N/A	INTRINSIC
Pfam:BCAS3	521	776	3.8e-35	PFAM
low complexity region	870	886	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000092822

SMART Domains

Protein: ENSMUSP00000090497
Gene: ENSMUSG00000059439

Domain	Start	End	E-Value	Type
low complexity region	3	17	N/A	INTRINSIC
low complexity region	28	37	N/A	INTRINSIC
Pfam:BCAS3	44	298	1.2e-35	PFAM
low complexity region	415	431	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000108056
AA Change: V431I

PolyPhen 2 Score 0.076 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000103691
Gene: ENSMUSG00000059439
AA Change: V431I

Domain	Start	End	E-Value	Type
Blast:WD40	56	104	7e-18	BLAST
WD40	340	380	7.7e-1	SMART
WD40	390	433	2.47e1	SMART
low complexity region	480	494	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000108061
AA Change: V431I

PolyPhen 2 Score 0.115 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000103696
Gene: ENSMUSG00000059439
AA Change: V431I

Domain	Start	End	E-Value	Type
Blast:WD40	56	104	2e-17	BLAST
WD40	340	380	7.7e-1	SMART
WD40	390	433	2.47e1	SMART
low complexity region	480	494	N/A	INTRINSIC
low complexity region	505	514	N/A	INTRINSIC
Pfam:BCAS3	521	789	1e-33	PFAM
low complexity region	899	913	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000108062
AA Change: V431I

PolyPhen 2 Score 0.115 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000103697
Gene: ENSMUSG00000059439
AA Change: V431I

Domain	Start	End	E-Value	Type
Blast:WD40	56	104	2e-17	BLAST
WD40	340	380	7.7e-1	SMART
WD40	390	433	2.47e1	SMART
low complexity region	480	494	N/A	INTRINSIC
low complexity region	505	514	N/A	INTRINSIC
Pfam:BCAS3	521	796	1.3e-28	PFAM
low complexity region	899	913	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000142596

SMART Domains

Protein: ENSMUSP00000122571
Gene: ENSMUSG00000059439

Domain	Start	End	E-Value	Type
low complexity region	7	21	N/A	INTRINSIC
low complexity region	32	41	N/A	INTRINSIC
Pfam:BCAS3	48	323	3e-29	PFAM
low complexity region	434	450	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000154396
AA Change: V210I

SMART Domains

Protein: ENSMUSP00000122154
Gene: ENSMUSG00000059439
AA Change: V210I

Domain	Start	End	E-Value	Type
WD40	120	160	7.7e-1	SMART
WD40	170	213	2.47e1	SMART
low complexity region	260	274	N/A	INTRINSIC
low complexity region	285	294	N/A	INTRINSIC
Pfam:BCAS3	301	561	1e-30	PFAM
low complexity region	650	666	N/A	INTRINSIC

Coding Region Coverage

1x: 99.8%
3x: 99.5%
10x: 98.4%
20x: 96.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 126 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
7530416G11Rik	T	A	15: 85,378,508 (GRCm39)	E45V	unknown	Homo
A530064D06Rik	GTAGGAAGCTTAG	GTAG	17: 48,470,549 (GRCm39)		probably benign	Homo
Anxa2	C	CCCA	9: 69,387,492 (GRCm39)		probably benign	Het
Apol6	GTTT	GTTTTTTT	15: 76,935,638 (GRCm39)		probably null	Het
Arrb2	C	T	11: 70,329,497 (GRCm39)	T269M	probably damaging	Homo
AY761185	CACTGTGGG	C	8: 21,433,919 (GRCm39)		probably null	Het
Blm	TACC	TACCGACC	7: 80,113,518 (GRCm39)		probably null	Het
Btnl10	AGA	AGAGGA	11: 58,814,755 (GRCm39)		probably benign	Homo
Btnl4	T	A	17: 34,691,610 (GRCm39)	K293M	probably benign	Het
Catsper2	TGTC	TGTCGTC	2: 121,228,260 (GRCm39)		probably benign	Het
Ccdc121	GAGAAG	GAG	5: 31,644,717 (GRCm39)		probably benign	Het
Cd109	ATTTAT	ATTTATTTATTTCTTTAT	9: 78,619,811 (GRCm39)		probably benign	Het
Cd164	G	T	10: 41,397,922 (GRCm39)	A59S	probably benign	Homo
Cd22	C	T	7: 30,577,507 (GRCm39)	R2H	possibly damaging	Homo
Chd4	C	T	6: 125,099,096 (GRCm39)	P1597L	probably benign	Homo
Chd4	CCCCTGCCCCTGCCACTGCCCCTGCC	CCCCTGCCCCTGCCCCTGCCACTGCCCCTGCC	6: 125,099,102 (GRCm39)		probably benign	Homo
Chga	AGC	AGCTGC	12: 102,527,661 (GRCm39)		probably benign	Het
Cluh	AGCC	AGCCTGGGCC	11: 74,560,357 (GRCm39)		probably benign	Het
Cnpy3	ACCC	ACCCCCC	17: 47,047,665 (GRCm39)		probably benign	Het
Cntnap1	CCCAGC	CCCAGCTCCAGC	11: 101,080,392 (GRCm39)		probably benign	Het
Cntnap1	AGCCCC	AGCCCCCGCCCC	11: 101,080,407 (GRCm39)		probably benign	Het
Cntnap1	CAGCCC	CAGCCCGAGCCC	11: 101,080,406 (GRCm39)		probably benign	Het
Cntnap1	AGCCCC	AGCCCCCGCCCC	11: 101,080,401 (GRCm39)		probably benign	Het
Col2a1	C	A	15: 97,886,862 (GRCm39)		probably null	Het
Col6a5	A	T	9: 105,811,373 (GRCm39)	N715K	unknown	Homo
Cttnbp2	ATT	ATTTCTGTT	6: 18,367,457 (GRCm39)		probably benign	Het
Dbr1	AGGAGG	AGGAGGGGGAGG	9: 99,465,730 (GRCm39)		probably benign	Het
Dbr1	AGGAGG	AGGAGGGGGAGG	9: 99,465,733 (GRCm39)		probably benign	Het
Dbr1	AGGAGG	AGGAGGCGGAGG	9: 99,465,736 (GRCm39)		probably benign	Het
Dbr1	GGAGGA	GGAGGAAGAGGA	9: 99,465,749 (GRCm39)		probably benign	Het
Dclre1a	AGGCTTTG	AG	19: 56,532,555 (GRCm39)		probably benign	Het
Dcpp1	A	C	17: 24,100,428 (GRCm39)	K53Q	probably benign	Het
Dhx8	CGAGAC	CGAGACAGAGAC	11: 101,629,014 (GRCm39)		probably benign	Homo
Dnaaf9	CC	CCTGC	2: 130,612,672 (GRCm39)		probably benign	Het
Dnaaf9	TCC	TCCCCC	2: 130,612,665 (GRCm39)		probably benign	Het
Dnah12	G	T	14: 26,571,342 (GRCm39)	G2817V	probably damaging	Homo
Dthd1	C	CTT	5: 63,000,369 (GRCm39)		probably null	Homo
Efhd2	GCCGCC	GCCGCCTCCGCC	4: 141,602,075 (GRCm39)		probably benign	Het
Eps8	AC	ACTCGC	6: 137,494,067 (GRCm39)		probably null	Het
Ermn	TTC	TTCATC	2: 57,938,081 (GRCm39)		probably benign	Het
Fam81b	TC	TCTCC	13: 76,419,442 (GRCm39)		probably benign	Het
Fbrsl1	TG	TGCGTGTGCTGGCG	5: 110,526,016 (GRCm39)		probably benign	Het
Fbxo43	CCTGTG	CCTGTGTCTGTG	15: 36,152,247 (GRCm39)		probably benign	Het
Fbxo43	GCCTGT	GCCTGTTCCTGT	15: 36,152,246 (GRCm39)		probably benign	Het
Fmn1	CTCCTC	CTCCTCTTCCTC	2: 113,356,118 (GRCm39)		probably benign	Het
Fmn1	TCCTCC	TCCTCCCCCTCC	2: 113,356,119 (GRCm39)		probably benign	Het
Frmpd2	G	T	14: 33,232,978 (GRCm39)	L399F	probably damaging	Homo
Gabre	GCTCCGACTCCGACTCCG	GCTCCGACTCCGACTCCGACTCCG	X: 71,313,636 (GRCm39)		probably benign	Homo
Gabre	ACTCCGGCTCCGGCTCAGGCTCAGGCTCCGGCTCAGGCTCCGGCTCCGGCTCCGGCTC	ACTCCGGCTCCGGCTCCGGCTCAGGCTCAGGCTCCGGCTCAGGCTCCGGCTCCGGCTCCGGCTC	X: 71,313,648 (GRCm39)		probably benign	Homo
Gbp2b	A	G	3: 142,309,413 (GRCm39)	I175V	probably benign	Het
Gm4340	AG	AGCCG	10: 104,031,961 (GRCm39)		probably benign	Het
Gm4340	CAG	CAGTAG	10: 104,031,939 (GRCm39)		probably null	Het
Gm4340	AGC	AGCCGC	10: 104,031,940 (GRCm39)		probably benign	Het
H2-Q4	G	A	17: 35,599,381 (GRCm39)	D155N	probably damaging	Het
Ighv5-9	C	T	12: 113,625,497 (GRCm39)	S82N	probably benign	Homo
Ipo9	TCC	TCCCCC	1: 135,314,019 (GRCm39)		probably benign	Het
Ipo9	CCATC	CCATCATC	1: 135,314,004 (GRCm39)		probably benign	Het
Isg20l2	GAAA	GAAAAAA	3: 87,839,024 (GRCm39)		probably benign	Homo
Klra9	C	G	6: 130,159,366 (GRCm39)	D216H	probably benign	Het
Kmt2b	TCC	TCCTCCGCC	7: 30,285,806 (GRCm39)		probably benign	Het
Kmt2b	CCTCCT	CCTCCTGCTCCT	7: 30,285,786 (GRCm39)		probably benign	Het
Kmt2b	CCTCCT	CCTCCTACTCCT	7: 30,285,789 (GRCm39)		probably null	Het
Krt10	TCC	TCCGCCGCC	11: 99,280,102 (GRCm39)		probably benign	Het
Las1l	TC	TCTTCCAC	X: 94,984,231 (GRCm39)		probably benign	Het
Las1l	TCTTCC	TCTTCCGCTTCC	X: 94,984,225 (GRCm39)		probably benign	Het
Las1l	TTCCTCCTCCTC	TTCCTC	X: 94,984,227 (GRCm39)		probably benign	Het
Lce1m	TGCTGCCACC	TGCTGCCACCACGGCTGCCACC	3: 92,925,575 (GRCm39)		probably benign	Homo
Loricrin	GCCGCCGCC	GC	3: 91,989,201 (GRCm39)		probably null	Het
Lrit3	AC	ACATCC	3: 129,597,562 (GRCm39)		probably null	Het
Lrrc63	CGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG	CGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG	14: 75,362,622 (GRCm39)		probably benign	Homo
Mapk7	GG	GGTGCTAG	11: 61,381,048 (GRCm39)		probably benign	Het
Med12l	GCAACA	GCAACAACA	3: 59,183,377 (GRCm39)		probably benign	Het
Nacad	GGGTCA	GGGTCATGGTCA	11: 6,549,753 (GRCm39)		probably benign	Het
Ndufc2	G	C	7: 97,049,497 (GRCm39)	M34I	probably benign	Het
Nphp3	CACG	C	9: 103,903,138 (GRCm39)		probably benign	Het
Nrg3	AG	AGCCTTTG	14: 38,119,223 (GRCm39)		probably benign	Het
Pdik1l	TTTTGTTTT	TTTTGTTTTGTGTTTGTTTT	4: 134,006,680 (GRCm39)		probably null	Homo
Pdik1l	TTTTTGTTTT	TTTTTGTTTTGATTTTGTTTT	4: 134,006,679 (GRCm39)		probably null	Homo
Phaf1	G	A	8: 105,967,730 (GRCm39)	G207E	probably benign	Homo
Plekhs1	AC	ACCTCCCCCGAGGC	19: 56,468,295 (GRCm39)		probably benign	Het
Prag1	CCGC	CCGCCGC	8: 36,571,037 (GRCm39)		probably benign	Homo
Prtg	G	A	9: 72,764,147 (GRCm39)	R540Q	probably damaging	Het
Raet1d	T	TCCTCTCTGGTAG	10: 22,246,817 (GRCm39)		probably null	Homo
Rhbdf1	A	ATTTT	11: 32,164,391 (GRCm39)		probably benign	Het
Rps19	AAAATT	AAAATTGAAATT	7: 24,588,607 (GRCm39)		probably benign	Het
Rtbdn	GGCAGC	GGCAGCCGCAGC	8: 85,682,800 (GRCm39)		probably benign	Het
Scaf4	TGCGGC	TGC	16: 90,026,742 (GRCm39)		probably benign	Homo
Serac1	T	A	17: 6,121,083 (GRCm39)	K70N	probably damaging	Homo
Setd1a	CTGGTGGTGGTGGTGGTGGTAGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG	CTGGTGGTGGTGGTGGTGGTGGTGGTGGTAGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG	7: 127,384,469 (GRCm39)		probably benign	Het
Shf	TCT	TCTGCT	2: 122,184,658 (GRCm39)		probably benign	Homo
Shroom4	TGCAGCAGCAGCAGCAGCA	TGCAGCAGCAGCAGCA	X: 6,536,115 (GRCm39)		probably benign	Homo
Six3	CGG	CGGAGG	17: 85,928,793 (GRCm39)		probably benign	Het
Snx1	TCT	TCTCCT	9: 66,012,208 (GRCm39)		probably benign	Homo
Spag17	AGG	AGGGGG	3: 99,963,561 (GRCm39)		probably benign	Het
Spag17	GGA	GGATGA	3: 99,963,574 (GRCm39)		probably benign	Het
Speer4a1	C	A	5: 26,241,746 (GRCm39)	E127*	probably null	Het
Sry	ACTG	ACTGCTG	Y: 2,662,818 (GRCm39)		probably benign	Het
Supt20	A	AGCAGCT	3: 54,635,092 (GRCm39)		probably benign	Het
Supt20	GCAGCA	GCAGCATCAGCA	3: 54,635,072 (GRCm39)		probably benign	Het
Supt20	CAGCAG	CAGCAGGAGCAG	3: 54,635,076 (GRCm39)		probably benign	Het
Tcof1	GGGTA	G	18: 60,961,722 (GRCm39)		probably benign	Homo
Tert	C	CAAGGGTGCG	13: 73,796,423 (GRCm39)		probably benign	Het
Tmbim7	C	T	5: 3,720,064 (GRCm39)	R100C	possibly damaging	Het
Tmed6	C	CTAGA	8: 107,788,230 (GRCm39)		probably null	Homo
Tob1	CACA	CACAACA	11: 94,105,277 (GRCm39)		probably benign	Het
Tob1	CA	CAGCAA	11: 94,105,303 (GRCm39)		probably null	Het
Trcg1	AGCTCCTGTGTCTGT	A	9: 57,149,485 (GRCm39)		probably null	Homo
Trim16	A	AAGC	11: 62,711,521 (GRCm39)		probably benign	Homo
Tsbp1	GCA	GCACCA	17: 34,679,027 (GRCm39)		probably benign	Het
Tsbp1	AGC	AGCCGC	17: 34,679,047 (GRCm39)		probably benign	Het
Tubgcp4	GTGA	G	2: 121,005,944 (GRCm39)		probably benign	Het
Tusc1	ACCGCC	ACCGCCCCCGCC	4: 93,223,544 (GRCm39)		probably benign	Het
Ubtf	CTTC	CTTCTTC	11: 102,197,771 (GRCm39)		probably benign	Het
Ubtf	CTCTTC	CTCTTCTTC	11: 102,197,769 (GRCm39)		probably benign	Het
Vars1	GTGG	GTGGAGTCCTGGTTGG	17: 35,234,964 (GRCm39)		probably benign	Homo
Vmn2r52	C	T	7: 9,892,947 (GRCm39)	E731K	probably damaging	Het
Vmn2r87	C	T	10: 130,314,583 (GRCm39)	M334I	probably benign	Homo
Zc3h13	GTGCGAGAT	GTGCGAGATTTGCGAGAT	14: 75,561,038 (GRCm39)		probably benign	Het
Zc3h13	CGGGATGTGCG	CGGGATGTGCGGGATGTGCG	14: 75,561,032 (GRCm39)		probably benign	Homo
Zc3h13	TGTGCGAG	TGTGCGAGGAGTGCGAG	14: 75,561,037 (GRCm39)		probably benign	Het
Zfhx3	CAGCA	CAGCAACAGAAGCA	8: 109,682,733 (GRCm39)		probably benign	Het
Zfp282	GGC	GGCAGC	6: 47,881,725 (GRCm39)		probably benign	Het
Zfp459	GA	GAGTTA	13: 67,556,394 (GRCm39)		probably null	Homo
Zfp598	ACCACC	ACCACCCCCACC	17: 24,899,753 (GRCm39)		probably benign	Het
Zfp831	TCC	TCCCCC	2: 174,487,261 (GRCm39)		probably benign	Het
Zfp997	G	A	13: 66,270,022 (GRCm39)	S396N	probably benign	Het

Other mutations in Bcas3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00340:Bcas3	APN	11	85,256,417 (GRCm39)	missense	probably damaging	0.98
IGL00754:Bcas3	APN	11	85,386,649 (GRCm39)	splice site	probably benign
IGL01712:Bcas3	APN	11	85,471,874 (GRCm39)	missense	probably damaging	0.99
IGL02073:Bcas3	APN	11	85,448,263 (GRCm39)	missense	probably damaging	1.00
IGL02261:Bcas3	APN	11	85,422,756 (GRCm39)	missense	probably damaging	1.00
IGL02323:Bcas3	APN	11	85,386,671 (GRCm39)	missense	probably damaging	0.97
IGL02493:Bcas3	APN	11	85,386,708 (GRCm39)	missense	probably damaging	0.99
IGL02609:Bcas3	APN	11	85,348,720 (GRCm39)	missense	probably damaging	1.00
IGL02808:Bcas3	APN	11	85,386,677 (GRCm39)	missense	probably benign	0.02
IGL03085:Bcas3	APN	11	85,367,609 (GRCm39)	missense	probably damaging	1.00
IGL03263:Bcas3	APN	11	85,712,948 (GRCm39)	intron	probably benign
FR4340:Bcas3	UTSW	11	85,400,323 (GRCm39)	missense	probably benign	0.12
FR4342:Bcas3	UTSW	11	85,400,323 (GRCm39)	missense	probably benign	0.12
IGL02991:Bcas3	UTSW	11	85,348,713 (GRCm39)	nonsense	probably null
PIT4377001:Bcas3	UTSW	11	85,386,668 (GRCm39)	missense	probably damaging	0.98
PIT4472001:Bcas3	UTSW	11	85,422,726 (GRCm39)	missense	probably damaging	0.99
R0145:Bcas3	UTSW	11	85,250,436 (GRCm39)	splice site	probably benign
R0257:Bcas3	UTSW	11	85,712,865 (GRCm39)	missense	probably benign	0.00
R0276:Bcas3	UTSW	11	85,361,663 (GRCm39)	critical splice donor site	probably null
R0485:Bcas3	UTSW	11	85,386,676 (GRCm39)	missense	probably damaging	0.99
R1053:Bcas3	UTSW	11	85,448,236 (GRCm39)	missense	probably benign	0.10
R1833:Bcas3	UTSW	11	85,474,775 (GRCm39)	missense	probably benign	0.00
R2107:Bcas3	UTSW	11	85,348,704 (GRCm39)	missense	probably damaging	0.97
R2108:Bcas3	UTSW	11	85,348,704 (GRCm39)	missense	probably damaging	0.97
R2215:Bcas3	UTSW	11	85,692,769 (GRCm39)	missense	probably damaging	0.99
R2404:Bcas3	UTSW	11	85,245,715 (GRCm39)	splice site	probably benign
R2413:Bcas3	UTSW	11	85,422,681 (GRCm39)	missense	probably damaging	1.00
R3694:Bcas3	UTSW	11	85,692,628 (GRCm39)	missense	probably benign	0.00
R3880:Bcas3	UTSW	11	85,261,948 (GRCm39)	missense	probably benign	0.02
R4241:Bcas3	UTSW	11	85,361,652 (GRCm39)	missense	probably damaging	0.99
R4794:Bcas3	UTSW	11	85,400,294 (GRCm39)	missense	probably damaging	1.00
R5035:Bcas3	UTSW	11	85,434,771 (GRCm39)	missense	probably damaging	1.00
R5073:Bcas3	UTSW	11	85,261,958 (GRCm39)	missense	probably damaging	1.00
R5245:Bcas3	UTSW	11	85,449,912 (GRCm39)	missense	probably damaging	1.00
R5358:Bcas3	UTSW	11	85,342,581 (GRCm39)	missense	probably benign	0.02
R5395:Bcas3	UTSW	11	85,716,075 (GRCm39)	missense	probably damaging	0.99
R5615:Bcas3	UTSW	11	85,361,587 (GRCm39)	missense	probably damaging	1.00
R5753:Bcas3	UTSW	11	85,712,910 (GRCm39)	intron	probably benign
R6198:Bcas3	UTSW	11	85,400,261 (GRCm39)	missense	probably damaging	0.99
R6668:Bcas3	UTSW	11	85,692,677 (GRCm39)	missense	probably damaging	0.98
R7170:Bcas3	UTSW	11	85,386,744 (GRCm39)	missense	probably damaging	0.96
R7171:Bcas3	UTSW	11	85,474,763 (GRCm39)	missense	probably damaging	1.00
R7672:Bcas3	UTSW	11	85,286,213 (GRCm39)	nonsense	probably null
R7689:Bcas3	UTSW	11	85,386,713 (GRCm39)	missense	probably benign	0.10
R7912:Bcas3	UTSW	11	85,261,954 (GRCm39)	missense	probably damaging	1.00
R8260:Bcas3	UTSW	11	85,400,372 (GRCm39)	missense	possibly damaging	0.50
R8292:Bcas3	UTSW	11	85,348,729 (GRCm39)	missense	probably damaging	0.99
R8334:Bcas3	UTSW	11	85,467,637 (GRCm39)	missense	possibly damaging	0.60
R8716:Bcas3	UTSW	11	85,471,868 (GRCm39)	missense	probably damaging	1.00
R8812:Bcas3	UTSW	11	85,449,973 (GRCm39)	missense	probably benign
R9300:Bcas3	UTSW	11	85,448,184 (GRCm39)	missense	probably damaging	1.00
R9709:Bcas3	UTSW	11	85,474,749 (GRCm39)	missense	probably damaging	1.00
V3553:Bcas3	UTSW	11	85,712,926 (GRCm39)	intron	probably benign
X0020:Bcas3	UTSW	11	85,422,634 (GRCm39)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- AATCTGCTAAAGAGGCAACCATTTC -3'
(R):5'- TCTACTGGAAAGCAGCATGAG -3'

Sequencing Primer
(F):5'- AGAGGCAACCATTTCTATTTGC -3'
(R):5'- AAGTGTTTACCATGCAGCGG -3'

Posted On

2018-04-05