Mutagenetix > Incidental Mutation

Incidental Mutation 'R6353:Vmn2r16'

512291

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r16

Ensembl Gene

ENSMUSG00000092080

Gene Name

vomeronasal 2, receptor 16

Synonyms

EG384220

MMRRC Submission

044505-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.154) question?

Stock #

R6353 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

109478247-109512347 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 109488119 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Aspartic acid at position 331 (N331D)

Ref Sequence

ENSEMBL: ENSMUSP00000127838 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165180]

AlphaFold

A0A3B2WCY4

Predicted Effect

probably benign
Transcript: ENSMUST00000165180
AA Change: N331D

PolyPhen 2 Score 0.331 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000127838
Gene: ENSMUSG00000092080
AA Change: N331D

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	76	425	2.3e-28	PFAM
Pfam:NCD3G	509	563	8.2e-19	PFAM
Pfam:7tm_3	596	831	3.5e-56	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.8%
20x: 93.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930550C14Rik	C	A	9: 53,325,642 (GRCm39)	Q60K	probably benign	Het
Aadacl2fm2	A	T	3: 59,659,529 (GRCm39)	L327F	probably damaging	Het
Aamp	T	C	1: 74,319,987 (GRCm39)	D397G	probably benign	Het
Aco1	G	A	4: 40,186,367 (GRCm39)	R593Q	probably benign	Het
Acta1	T	C	8: 124,620,426 (GRCm39)	E4G	probably benign	Het
Apob	A	T	12: 8,059,421 (GRCm39)	K2601N	probably damaging	Het
Arpin	C	A	7: 79,585,093 (GRCm39)		probably benign	Het
Asnsd1	T	C	1: 53,386,938 (GRCm39)	I230V	probably benign	Het
Baiap2l1	C	T	5: 144,218,898 (GRCm39)	E237K	possibly damaging	Het
Chek1	T	C	9: 36,635,255 (GRCm39)	K43E	probably benign	Het
Clec2g	T	A	6: 128,959,895 (GRCm39)		probably null	Het
Cnot10	A	G	9: 114,426,614 (GRCm39)	L646P	probably damaging	Het
Cyp2j9	T	C	4: 96,474,135 (GRCm39)	T102A	probably benign	Het
Dnase1l2	A	T	17: 24,661,219 (GRCm39)	L30Q	probably damaging	Het
Edc3	T	C	9: 57,623,520 (GRCm39)	S152P	probably benign	Het
Gask1b	A	T	3: 79,848,647 (GRCm39)	R464S	probably damaging	Het
Gpr160	A	T	3: 30,950,171 (GRCm39)	D81V	probably damaging	Het
Intu	A	T	3: 40,608,138 (GRCm39)	D32V	probably damaging	Het
Itga5	T	C	15: 103,260,950 (GRCm39)	E512G	probably damaging	Het
Khnyn	T	C	14: 56,131,760 (GRCm39)	F561L	possibly damaging	Het
Kmt2e	T	A	5: 23,698,243 (GRCm39)	V645E	probably damaging	Het
Mcoln3	T	C	3: 145,836,909 (GRCm39)	F247S	probably damaging	Het
Nek9	G	A	12: 85,348,603 (GRCm39)	T977I	probably damaging	Het
Nphs1	A	G	7: 30,173,969 (GRCm39)	T1015A	probably damaging	Het
Ntmt2	A	G	1: 163,531,680 (GRCm39)	Y158H	possibly damaging	Het
Nudcd1	T	C	15: 44,284,158 (GRCm39)	Y76C	probably damaging	Het
Or2a54	C	A	6: 43,093,070 (GRCm39)	Y131*	probably null	Het
Or8b44	T	C	9: 38,410,112 (GRCm39)	I49T	probably benign	Het
Pgd	C	T	4: 149,245,209 (GRCm39)		probably null	Het
Prl7c1	A	G	13: 27,957,709 (GRCm39)	S244P	possibly damaging	Het
Rpap1	G	T	2: 119,607,377 (GRCm39)		probably null	Het
Rrp8	G	A	7: 105,383,325 (GRCm39)	R314*	probably null	Het
Smarca4	T	A	9: 21,590,445 (GRCm39)		probably null	Het
Stambpl1	A	G	19: 34,211,520 (GRCm39)		probably null	Het
Tmeff2	T	C	1: 51,220,985 (GRCm39)	V320A	probably damaging	Het
Top2b	A	G	14: 16,416,671 (GRCm38)	K83E	probably damaging	Het
Ttc39b	C	G	4: 83,148,730 (GRCm39)	V560L	probably benign	Het
Ttn	A	T	2: 76,672,153 (GRCm39)		probably benign	Het
Usp32	T	C	11: 84,913,107 (GRCm39)	I934V	probably benign	Het
Uxs1	T	C	1: 43,836,410 (GRCm39)	I122V	probably damaging	Het
Vmn1r168	A	G	7: 23,240,944 (GRCm39)	N267S	probably benign	Het

Other mutations in Vmn2r16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01116:Vmn2r16	APN	5	109,488,294 (GRCm39)	missense	probably damaging	1.00
IGL01374:Vmn2r16	APN	5	109,478,283 (GRCm39)	missense	probably benign	0.00
IGL01391:Vmn2r16	APN	5	109,511,627 (GRCm39)	missense	possibly damaging	0.50
IGL01419:Vmn2r16	APN	5	109,510,267 (GRCm39)	splice site	probably benign
IGL01634:Vmn2r16	APN	5	109,488,177 (GRCm39)	missense	probably benign	0.00
IGL01845:Vmn2r16	APN	5	109,511,762 (GRCm39)	missense	probably damaging	1.00
IGL01875:Vmn2r16	APN	5	109,478,277 (GRCm39)	missense	probably benign	0.01
IGL01910:Vmn2r16	APN	5	109,487,951 (GRCm39)	missense	probably damaging	1.00
IGL02217:Vmn2r16	APN	5	109,487,676 (GRCm39)	missense	probably damaging	0.98
IGL02327:Vmn2r16	APN	5	109,487,987 (GRCm39)	missense	probably benign	0.01
IGL02491:Vmn2r16	APN	5	109,487,703 (GRCm39)	nonsense	probably null
IGL02531:Vmn2r16	APN	5	109,488,134 (GRCm39)	missense	probably damaging	0.99
IGL02680:Vmn2r16	APN	5	109,487,948 (GRCm39)	missense	probably benign	0.44
IGL02884:Vmn2r16	APN	5	109,508,757 (GRCm39)	missense	possibly damaging	0.94
IGL03084:Vmn2r16	APN	5	109,478,292 (GRCm39)	missense	probably damaging	0.99
IGL03096:Vmn2r16	APN	5	109,487,751 (GRCm39)	missense	probably damaging	0.99
IGL03355:Vmn2r16	APN	5	109,511,580 (GRCm39)	missense	possibly damaging	0.74
R0280:Vmn2r16	UTSW	5	109,488,005 (GRCm39)	missense	possibly damaging	0.88
R0594:Vmn2r16	UTSW	5	109,511,762 (GRCm39)	missense	probably damaging	1.00
R1016:Vmn2r16	UTSW	5	109,487,754 (GRCm39)	missense	probably damaging	1.00
R1109:Vmn2r16	UTSW	5	109,487,652 (GRCm39)	missense	probably damaging	0.98
R1248:Vmn2r16	UTSW	5	109,508,643 (GRCm39)	missense	probably benign	0.10
R1626:Vmn2r16	UTSW	5	109,511,443 (GRCm39)	missense	probably damaging	1.00
R1909:Vmn2r16	UTSW	5	109,511,853 (GRCm39)	missense	probably benign	0.01
R1929:Vmn2r16	UTSW	5	109,487,124 (GRCm39)	missense	possibly damaging	0.92
R1982:Vmn2r16	UTSW	5	109,511,890 (GRCm39)	missense	probably benign	0.01
R3038:Vmn2r16	UTSW	5	109,487,199 (GRCm39)	missense	probably damaging	1.00
R3437:Vmn2r16	UTSW	5	109,478,362 (GRCm39)	missense	probably damaging	0.99
R3734:Vmn2r16	UTSW	5	109,478,280 (GRCm39)	missense	probably benign	0.11
R3820:Vmn2r16	UTSW	5	109,510,143 (GRCm39)	missense	probably benign	0.36
R3873:Vmn2r16	UTSW	5	109,488,177 (GRCm39)	missense	probably benign	0.33
R4165:Vmn2r16	UTSW	5	109,478,427 (GRCm39)	missense	possibly damaging	0.80
R4373:Vmn2r16	UTSW	5	109,511,667 (GRCm39)	missense	probably damaging	1.00
R4575:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4576:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4578:Vmn2r16	UTSW	5	109,511,665 (GRCm39)	missense	possibly damaging	0.81
R4637:Vmn2r16	UTSW	5	109,478,280 (GRCm39)	missense	probably benign	0.00
R4696:Vmn2r16	UTSW	5	109,487,168 (GRCm39)	missense	probably benign	0.01
R5026:Vmn2r16	UTSW	5	109,508,722 (GRCm39)	nonsense	probably null
R5180:Vmn2r16	UTSW	5	109,478,391 (GRCm39)	missense	probably benign	0.01
R5433:Vmn2r16	UTSW	5	109,511,708 (GRCm39)	missense	probably damaging	1.00
R5955:Vmn2r16	UTSW	5	109,511,613 (GRCm39)	missense	possibly damaging	0.78
R5958:Vmn2r16	UTSW	5	109,510,153 (GRCm39)	missense	possibly damaging	0.81
R6389:Vmn2r16	UTSW	5	109,478,344 (GRCm39)	missense	probably benign	0.19
R6819:Vmn2r16	UTSW	5	109,488,412 (GRCm39)	missense	probably benign	0.04
R6994:Vmn2r16	UTSW	5	109,487,969 (GRCm39)	missense	probably damaging	1.00
R7061:Vmn2r16	UTSW	5	109,511,620 (GRCm39)	missense	probably damaging	0.99
R7063:Vmn2r16	UTSW	5	109,511,650 (GRCm39)	missense	probably damaging	1.00
R7220:Vmn2r16	UTSW	5	109,508,772 (GRCm39)	missense	probably damaging	0.97
R7268:Vmn2r16	UTSW	5	109,488,331 (GRCm39)	nonsense	probably null
R7420:Vmn2r16	UTSW	5	109,511,736 (GRCm39)	missense	probably damaging	0.96
R7591:Vmn2r16	UTSW	5	109,510,223 (GRCm39)	missense	probably damaging	0.99
R7644:Vmn2r16	UTSW	5	109,487,837 (GRCm39)	missense	probably damaging	1.00
R7939:Vmn2r16	UTSW	5	109,487,705 (GRCm39)	missense	possibly damaging	0.79
R7977:Vmn2r16	UTSW	5	109,488,015 (GRCm39)	missense	probably damaging	1.00
R7987:Vmn2r16	UTSW	5	109,488,015 (GRCm39)	missense	probably damaging	1.00
R8023:Vmn2r16	UTSW	5	109,488,272 (GRCm39)	nonsense	probably null
R8427:Vmn2r16	UTSW	5	109,488,138 (GRCm39)	missense	probably benign	0.03
R8436:Vmn2r16	UTSW	5	109,511,649 (GRCm39)	missense	probably damaging	1.00
R8475:Vmn2r16	UTSW	5	109,487,073 (GRCm39)	missense	probably benign	0.01
R8554:Vmn2r16	UTSW	5	109,511,997 (GRCm39)	missense	probably benign	0.13
R8771:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8774:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8774-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8775:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8775-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8777:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8777-TAIL:Vmn2r16	UTSW	5	109,488,231 (GRCm39)	missense	probably benign	0.00
R8825:Vmn2r16	UTSW	5	109,487,019 (GRCm39)	missense	probably benign	0.41
R8865:Vmn2r16	UTSW	5	109,487,910 (GRCm39)	missense	probably benign	0.33
R8900:Vmn2r16	UTSW	5	109,511,619 (GRCm39)	missense	probably benign	0.04
R9365:Vmn2r16	UTSW	5	109,488,064 (GRCm39)	missense	probably damaging	0.99
R9683:Vmn2r16	UTSW	5	109,511,677 (GRCm39)	missense	probably damaging	1.00
X0027:Vmn2r16	UTSW	5	109,487,175 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r16	UTSW	5	109,511,779 (GRCm39)	frame shift	probably null
Z1088:Vmn2r16	UTSW	5	109,488,381 (GRCm39)	missense	probably benign	0.03
Z1177:Vmn2r16	UTSW	5	109,487,864 (GRCm39)	missense	possibly damaging	0.79

Predicted Primers

PCR Primer
(F):5'- TGCAGATATACATGACAAGGGCTG -3'
(R):5'- ACTCAGGACCATGTCAATTTTGTG -3'

Sequencing Primer
(F):5'- GAATCAACAGCAAAGGTTGTTATC -3'
(R):5'- CAGGACCATGTCAATTTTGTGTTGTG -3'

Posted On

2018-04-27