Incidental Mutation 'R6353:Nek9'
ID 512308
Institutional Source Beutler Lab
Gene Symbol Nek9
Ensembl Gene ENSMUSG00000034290
Gene Name NIMA (never in mitosis gene a)-related expressed kinase 9
Synonyms C130021H08Rik
MMRRC Submission 044505-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6353 (G1)
Quality Score 206.009
Status Not validated
Chromosome 12
Chromosomal Location 85346288-85386136 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 85348603 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Isoleucine at position 977 (T977I)
Ref Sequence ENSEMBL: ENSMUSP00000049056 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000040992] [ENSMUST00000059341]
AlphaFold Q8K1R7
Predicted Effect probably damaging
Transcript: ENSMUST00000040992
AA Change: T977I

PolyPhen 2 Score 0.963 (Sensitivity: 0.78; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000049056
Gene: ENSMUSG00000034290
AA Change: T977I

DomainStartEndE-ValueType
low complexity region 19 49 N/A INTRINSIC
S_TKc 52 308 1.07e-73 SMART
Pfam:RCC1 389 441 1.2e-9 PFAM
Pfam:RCC1_2 428 457 1.5e-8 PFAM
Pfam:RCC1 444 495 3.6e-13 PFAM
Pfam:RCC1_2 482 511 3.6e-11 PFAM
Pfam:RCC1 499 547 7.6e-14 PFAM
Pfam:RCC1 615 665 4.2e-8 PFAM
Pfam:RCC1_2 652 681 4.4e-7 PFAM
low complexity region 752 767 N/A INTRINSIC
low complexity region 889 900 N/A INTRINSIC
low complexity region 910 927 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000059341
SMART Domains Protein: ENSMUSP00000051664
Gene: ENSMUSG00000045064

DomainStartEndE-ValueType
low complexity region 93 106 N/A INTRINSIC
coiled coil region 207 254 N/A INTRINSIC
Pfam:zf-C2HC_2 378 402 3.6e-10 PFAM
low complexity region 433 444 N/A INTRINSIC
Pfam:zf-C2HC_2 489 513 3.1e-11 PFAM
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.8%
  • 20x: 93.3%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression. [provided by RefSeq, Jul 2016]
Allele List at MGI
Other mutations in this stock
Total: 41 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930550C14Rik C A 9: 53,325,642 (GRCm39) Q60K probably benign Het
Aadacl2fm2 A T 3: 59,659,529 (GRCm39) L327F probably damaging Het
Aamp T C 1: 74,319,987 (GRCm39) D397G probably benign Het
Aco1 G A 4: 40,186,367 (GRCm39) R593Q probably benign Het
Acta1 T C 8: 124,620,426 (GRCm39) E4G probably benign Het
Apob A T 12: 8,059,421 (GRCm39) K2601N probably damaging Het
Arpin C A 7: 79,585,093 (GRCm39) probably benign Het
Asnsd1 T C 1: 53,386,938 (GRCm39) I230V probably benign Het
Baiap2l1 C T 5: 144,218,898 (GRCm39) E237K possibly damaging Het
Chek1 T C 9: 36,635,255 (GRCm39) K43E probably benign Het
Clec2g T A 6: 128,959,895 (GRCm39) probably null Het
Cnot10 A G 9: 114,426,614 (GRCm39) L646P probably damaging Het
Cyp2j9 T C 4: 96,474,135 (GRCm39) T102A probably benign Het
Dnase1l2 A T 17: 24,661,219 (GRCm39) L30Q probably damaging Het
Edc3 T C 9: 57,623,520 (GRCm39) S152P probably benign Het
Gask1b A T 3: 79,848,647 (GRCm39) R464S probably damaging Het
Gpr160 A T 3: 30,950,171 (GRCm39) D81V probably damaging Het
Intu A T 3: 40,608,138 (GRCm39) D32V probably damaging Het
Itga5 T C 15: 103,260,950 (GRCm39) E512G probably damaging Het
Khnyn T C 14: 56,131,760 (GRCm39) F561L possibly damaging Het
Kmt2e T A 5: 23,698,243 (GRCm39) V645E probably damaging Het
Mcoln3 T C 3: 145,836,909 (GRCm39) F247S probably damaging Het
Nphs1 A G 7: 30,173,969 (GRCm39) T1015A probably damaging Het
Ntmt2 A G 1: 163,531,680 (GRCm39) Y158H possibly damaging Het
Nudcd1 T C 15: 44,284,158 (GRCm39) Y76C probably damaging Het
Or2a54 C A 6: 43,093,070 (GRCm39) Y131* probably null Het
Or8b44 T C 9: 38,410,112 (GRCm39) I49T probably benign Het
Pgd C T 4: 149,245,209 (GRCm39) probably null Het
Prl7c1 A G 13: 27,957,709 (GRCm39) S244P possibly damaging Het
Rpap1 G T 2: 119,607,377 (GRCm39) probably null Het
Rrp8 G A 7: 105,383,325 (GRCm39) R314* probably null Het
Smarca4 T A 9: 21,590,445 (GRCm39) probably null Het
Stambpl1 A G 19: 34,211,520 (GRCm39) probably null Het
Tmeff2 T C 1: 51,220,985 (GRCm39) V320A probably damaging Het
Top2b A G 14: 16,416,671 (GRCm38) K83E probably damaging Het
Ttc39b C G 4: 83,148,730 (GRCm39) V560L probably benign Het
Ttn A T 2: 76,672,153 (GRCm39) probably benign Het
Usp32 T C 11: 84,913,107 (GRCm39) I934V probably benign Het
Uxs1 T C 1: 43,836,410 (GRCm39) I122V probably damaging Het
Vmn1r168 A G 7: 23,240,944 (GRCm39) N267S probably benign Het
Vmn2r16 A G 5: 109,488,119 (GRCm39) N331D probably benign Het
Other mutations in Nek9
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01431:Nek9 APN 12 85,361,361 (GRCm39) missense probably benign 0.23
IGL01595:Nek9 APN 12 85,361,194 (GRCm39) missense probably damaging 1.00
IGL01603:Nek9 APN 12 85,352,379 (GRCm39) missense probably damaging 1.00
IGL01893:Nek9 APN 12 85,383,174 (GRCm39) missense probably damaging 1.00
IGL02017:Nek9 APN 12 85,376,697 (GRCm39) missense probably damaging 1.00
IGL02197:Nek9 APN 12 85,354,704 (GRCm39) missense probably null
IGL02207:Nek9 APN 12 85,350,257 (GRCm39) nonsense probably null
IGL02749:Nek9 APN 12 85,352,281 (GRCm39) missense probably benign 0.02
IGL02756:Nek9 APN 12 85,358,110 (GRCm39) critical splice donor site probably null
IGL03343:Nek9 APN 12 85,350,383 (GRCm39) missense probably damaging 1.00
Rose_colored UTSW 12 85,350,311 (GRCm39) missense probably damaging 1.00
R0048:Nek9 UTSW 12 85,348,673 (GRCm39) missense probably benign 0.17
R0331:Nek9 UTSW 12 85,374,149 (GRCm39) splice site probably benign
R0499:Nek9 UTSW 12 85,348,657 (GRCm39) missense probably benign 0.09
R1484:Nek9 UTSW 12 85,348,622 (GRCm39) missense probably damaging 1.00
R1760:Nek9 UTSW 12 85,357,184 (GRCm39) missense probably benign 0.00
R1760:Nek9 UTSW 12 85,352,364 (GRCm39) missense possibly damaging 0.71
R1883:Nek9 UTSW 12 85,379,330 (GRCm39) missense probably damaging 1.00
R1884:Nek9 UTSW 12 85,379,330 (GRCm39) missense probably damaging 1.00
R1999:Nek9 UTSW 12 85,376,677 (GRCm39) missense probably damaging 1.00
R2046:Nek9 UTSW 12 85,367,481 (GRCm39) splice site probably benign
R2096:Nek9 UTSW 12 85,361,322 (GRCm39) missense probably benign 0.00
R2150:Nek9 UTSW 12 85,376,677 (GRCm39) missense probably damaging 1.00
R2368:Nek9 UTSW 12 85,376,661 (GRCm39) missense possibly damaging 0.89
R2570:Nek9 UTSW 12 85,379,320 (GRCm39) nonsense probably null
R4381:Nek9 UTSW 12 85,376,632 (GRCm39) missense probably damaging 1.00
R4570:Nek9 UTSW 12 85,367,508 (GRCm39) missense probably damaging 1.00
R4661:Nek9 UTSW 12 85,367,666 (GRCm39) missense possibly damaging 0.78
R4669:Nek9 UTSW 12 85,360,978 (GRCm39) missense probably benign 0.00
R4993:Nek9 UTSW 12 85,357,194 (GRCm39) missense probably damaging 1.00
R5071:Nek9 UTSW 12 85,374,233 (GRCm39) missense possibly damaging 0.70
R5090:Nek9 UTSW 12 85,376,616 (GRCm39) critical splice donor site probably null
R5248:Nek9 UTSW 12 85,355,751 (GRCm39) missense probably damaging 1.00
R5521:Nek9 UTSW 12 85,374,219 (GRCm39) missense probably benign 0.09
R5734:Nek9 UTSW 12 85,350,289 (GRCm39) missense probably benign
R6039:Nek9 UTSW 12 85,359,859 (GRCm39) missense probably benign 0.08
R6039:Nek9 UTSW 12 85,359,859 (GRCm39) missense probably benign 0.08
R6269:Nek9 UTSW 12 85,379,103 (GRCm39) splice site probably null
R6406:Nek9 UTSW 12 85,385,946 (GRCm39) missense probably damaging 0.97
R6744:Nek9 UTSW 12 85,376,703 (GRCm39) missense probably benign 0.09
R6922:Nek9 UTSW 12 85,350,311 (GRCm39) missense probably damaging 1.00
R7603:Nek9 UTSW 12 85,350,288 (GRCm39) missense probably benign 0.00
R7686:Nek9 UTSW 12 85,350,433 (GRCm39) missense probably benign 0.01
R7905:Nek9 UTSW 12 85,352,370 (GRCm39) missense probably damaging 1.00
R7964:Nek9 UTSW 12 85,385,787 (GRCm39) missense probably benign 0.02
R8165:Nek9 UTSW 12 85,350,417 (GRCm39) missense probably benign 0.01
R8330:Nek9 UTSW 12 85,376,727 (GRCm39) missense probably damaging 1.00
R8802:Nek9 UTSW 12 85,374,192 (GRCm39) missense probably benign 0.19
R8859:Nek9 UTSW 12 85,353,120 (GRCm39) missense probably damaging 1.00
R8997:Nek9 UTSW 12 85,367,565 (GRCm39) missense probably benign 0.00
R9055:Nek9 UTSW 12 85,348,616 (GRCm39) missense probably damaging 0.99
R9261:Nek9 UTSW 12 85,359,841 (GRCm39) missense probably damaging 1.00
R9661:Nek9 UTSW 12 85,361,253 (GRCm39) missense possibly damaging 0.96
X0052:Nek9 UTSW 12 85,368,801 (GRCm39) critical splice donor site probably null
Z1177:Nek9 UTSW 12 85,380,819 (GRCm39) nonsense probably null
Predicted Primers PCR Primer
(F):5'- GCAATGCCACTCTCCAAATG -3'
(R):5'- AAGCCTTCCGTAGACTGACAG -3'

Sequencing Primer
(F):5'- TGAACAAGGAAAGTGCTTTTGAGCC -3'
(R):5'- TGACAGCAAACCCTGAGAGGTC -3'
Posted On 2018-04-27