Mutagenetix > Incidental Mutation

Incidental Mutation 'R6360:Tnc'

513054

Institutional Source

Beutler Lab

Gene Symbol

Tnc

Ensembl Gene

ENSMUSG00000028364

Gene Name

tenascin C

Synonyms

cytotactin, C130033P17Rik, TN-C, hexabrachion, tenascin-C, Hxb, TN

MMRRC Submission

044510-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6360 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

63878022-63965252 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 63918970 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 1151 (Y1151C)

Ref Sequence

ENSEMBL: ENSMUSP00000103000 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030056] [ENSMUST00000107372] [ENSMUST00000107377]

AlphaFold

Q80YX1

Predicted Effect

probably damaging
Transcript: ENSMUST00000030056
AA Change: Y1151C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000030056
Gene: ENSMUSG00000028364
AA Change: Y1151C

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000102995
Gene: ENSMUSG00000028364
AA Change: Y1151C

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	2.75e0	SMART
FN3	1529	1608	3.4e-4	SMART
FN3	1619	1697	1.55e-7	SMART
FN3	1708	1785	1.53e-6	SMART
FN3	1796	1873	7.75e-8	SMART
FBG	1888	2098	4.08e-124	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000107377
AA Change: Y1151C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000103000
Gene: ENSMUSG00000028364
AA Change: Y1151C

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135042

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141428

Meta Mutation Damage Score

0.1944

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.2%

Validation Efficiency

98% (59/60)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]
PHENOTYPE: Mice homozygous for several different targeted mutations show variable behavioral and nervous system phenotypes such as abnormal circadian rhythm, anxiety behavior, novelty-induced activity, swimming, impaired synaptic plasticity, long term potentiation and serotonin and dopamine neurotransmission. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgap10	G	A	8: 77,985,831 (GRCm39)	Q657*	probably null	Het
Car15	T	C	16: 17,655,930 (GRCm39)	T560A	probably benign	Het
Cass4	C	T	2: 172,274,531 (GRCm39)	H769Y	probably damaging	Het
Cdh6	A	G	15: 13,041,546 (GRCm39)	I506T	possibly damaging	Het
Clstn3	G	T	6: 124,415,388 (GRCm39)	R659S	possibly damaging	Het
Cntnap5b	C	T	1: 100,359,461 (GRCm39)	R695*	probably null	Het
Cpsf1	CCCCTGCATGAGGCAGGTCCC	CCCC	15: 76,481,655 (GRCm39)		probably null	Het
Dab2ip	A	G	2: 35,600,278 (GRCm39)	H355R	probably benign	Het
Dennd2a	C	T	6: 39,470,076 (GRCm39)	A539T	probably benign	Het
Dnajc18	T	C	18: 35,819,762 (GRCm39)	E173G	probably damaging	Het
Dock7	T	C	4: 98,857,899 (GRCm39)	I1472V	probably benign	Het
Esco1	A	T	18: 10,574,931 (GRCm39)	F714I	probably damaging	Het
Fam107a	T	G	14: 8,299,619 (GRCm38)	H73P	probably damaging	Het
Flg	A	G	3: 93,197,908 (GRCm39)		probably benign	Het
Fyn	C	A	10: 39,402,879 (GRCm39)	T217K	possibly damaging	Het
Gbp9	T	C	5: 105,231,596 (GRCm39)	D330G	probably benign	Het
Gin1	T	C	1: 97,720,264 (GRCm39)	S509P	possibly damaging	Het
Gm17067	A	C	7: 42,357,906 (GRCm39)	S199A	probably benign	Het
Grk4	A	T	5: 34,831,881 (GRCm39)	K50M	probably damaging	Het
Inpp4b	A	G	8: 82,629,481 (GRCm39)	H272R	probably benign	Het
Ipo7	T	A	7: 109,626,336 (GRCm39)	L48Q	probably damaging	Het
Kbtbd2	A	G	6: 56,756,191 (GRCm39)	I515T	probably damaging	Het
Kcnu1	T	A	8: 26,351,208 (GRCm39)	S190R	possibly damaging	Het
Kpnb1	T	C	11: 97,064,096 (GRCm39)	N336S	probably benign	Het
Lbr	G	T	1: 181,659,720 (GRCm39)	D158E	probably benign	Het
Mphosph10	T	C	7: 64,039,703 (GRCm39)	Q89R	probably benign	Het
Nectin3	T	A	16: 46,231,472 (GRCm39)	T21S	probably benign	Het
Numb	C	A	12: 83,844,036 (GRCm39)	R383L	probably damaging	Het
Or2p2	A	T	13: 21,256,753 (GRCm39)	N239K	probably damaging	Het
Or9a7	G	T	6: 40,521,647 (GRCm39)	Q89K	possibly damaging	Het
Pcdhb11	A	T	18: 37,555,212 (GRCm39)	I181F	probably benign	Het
Pcgf2	T	A	11: 97,583,235 (GRCm39)		probably null	Het
Pdzd8	T	C	19: 59,289,415 (GRCm39)	T662A	probably benign	Het
Pex13	A	C	11: 23,605,690 (GRCm39)	V180G	probably benign	Het
Pira1	A	T	7: 3,739,503 (GRCm39)	L455Q	probably damaging	Het
Pkp4	T	A	2: 59,045,091 (GRCm39)	V22D	probably benign	Het
Prkn	T	C	17: 12,222,939 (GRCm39)	F363S	probably damaging	Het
Prpf4b	A	C	13: 35,085,416 (GRCm39)	D954A	probably damaging	Het
Rfx8	T	C	1: 39,720,125 (GRCm39)	I317V	probably benign	Het
Rnaseh2b	T	C	14: 62,598,868 (GRCm39)	S198P	probably damaging	Het
Rock1	A	T	18: 10,116,778 (GRCm39)	C453S	possibly damaging	Het
Saxo4	T	C	19: 10,456,845 (GRCm39)	N167D	probably damaging	Het
Scarf1	G	T	11: 75,406,495 (GRCm39)	G260W	probably damaging	Het
Scyl1	T	C	19: 5,810,599 (GRCm39)	E538G	probably damaging	Het
Sec14l5	A	T	16: 4,990,859 (GRCm39)	I267F	probably damaging	Het
Senp6	T	A	9: 80,021,088 (GRCm39)	V256D	probably benign	Het
Sf3b4	G	A	3: 96,084,044 (GRCm39)		probably benign	Het
Ssh1	T	C	5: 114,099,408 (GRCm39)		probably null	Het
Tas2r143	A	C	6: 42,377,769 (GRCm39)	M200L	probably benign	Het
Tbc1d22a	C	T	15: 86,098,830 (GRCm39)	P19S	probably damaging	Het
Tent5b	T	C	4: 133,214,067 (GRCm39)	F313L	probably damaging	Het
Tmc5	T	A	7: 118,233,189 (GRCm39)	M1K	probably null	Het
Tshz3	A	G	7: 36,468,866 (GRCm39)	E285G	probably damaging	Het
Txndc11	C	T	16: 10,902,656 (GRCm39)	V664M	probably damaging	Het
Ube2g1	A	T	11: 72,553,908 (GRCm39)	N20Y	probably damaging	Het
Ufl1	T	A	4: 25,265,476 (GRCm39)	I369L	probably benign	Het
Vwf	A	T	6: 125,660,489 (GRCm39)	T2666S	probably benign	Het
Yif1a	A	G	19: 5,142,369 (GRCm39)	M259V	probably benign	Het
Zbtb46	T	C	2: 181,033,248 (GRCm39)	D471G	probably damaging	Het

Other mutations in Tnc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00229:Tnc	APN	4	63,935,061 (GRCm39)	splice site	probably benign
IGL00531:Tnc	APN	4	63,889,390 (GRCm39)	splice site	probably benign
IGL00674:Tnc	APN	4	63,883,844 (GRCm39)	missense	probably damaging	1.00
IGL01015:Tnc	APN	4	63,935,571 (GRCm39)	missense	probably benign	0.19
IGL01090:Tnc	APN	4	63,918,317 (GRCm39)	missense	probably damaging	1.00
IGL01310:Tnc	APN	4	63,931,314 (GRCm39)	missense	probably benign	0.03
IGL01331:Tnc	APN	4	63,901,112 (GRCm39)	missense	probably damaging	0.99
IGL01393:Tnc	APN	4	63,932,291 (GRCm39)	splice site	probably benign
IGL01411:Tnc	APN	4	63,918,959 (GRCm39)	missense	probably damaging	0.96
IGL01472:Tnc	APN	4	63,924,656 (GRCm39)	missense	probably benign	0.00
IGL01552:Tnc	APN	4	63,888,645 (GRCm39)	missense	probably damaging	1.00
IGL01661:Tnc	APN	4	63,888,544 (GRCm39)	splice site	probably benign
IGL01669:Tnc	APN	4	63,918,938 (GRCm39)	missense	probably damaging	1.00
IGL01912:Tnc	APN	4	63,926,977 (GRCm39)	missense	probably damaging	1.00
IGL02028:Tnc	APN	4	63,884,909 (GRCm39)	splice site	probably benign
IGL02100:Tnc	APN	4	63,918,398 (GRCm39)	missense	possibly damaging	0.84
IGL02549:Tnc	APN	4	63,933,309 (GRCm39)	missense	probably damaging	1.00
IGL02642:Tnc	APN	4	63,883,816 (GRCm39)	splice site	probably benign
IGL02712:Tnc	APN	4	63,893,493 (GRCm39)	missense	probably damaging	1.00
IGL02876:Tnc	APN	4	63,933,338 (GRCm39)	missense	possibly damaging	0.56
IGL02886:Tnc	APN	4	63,918,344 (GRCm39)	missense	probably damaging	0.96
IGL02972:Tnc	APN	4	63,894,715 (GRCm39)	missense	probably benign	0.11
IGL03073:Tnc	APN	4	63,889,461 (GRCm39)	missense	possibly damaging	0.58
IGL03116:Tnc	APN	4	63,932,270 (GRCm39)	missense	probably damaging	1.00
IGL03181:Tnc	APN	4	63,885,543 (GRCm39)	missense	possibly damaging	0.95
IGL03358:Tnc	APN	4	63,935,852 (GRCm39)	nonsense	probably null
tancredo	UTSW	4	63,911,534 (GRCm39)	nonsense	probably null
BB009:Tnc	UTSW	4	63,926,857 (GRCm39)	missense	probably benign
BB019:Tnc	UTSW	4	63,926,857 (GRCm39)	missense	probably benign
P0020:Tnc	UTSW	4	63,927,094 (GRCm39)	missense	possibly damaging	0.63
PIT4377001:Tnc	UTSW	4	63,935,973 (GRCm39)	missense	probably damaging	1.00
PIT4403001:Tnc	UTSW	4	63,882,904 (GRCm39)	missense	probably damaging	1.00
PIT4468001:Tnc	UTSW	4	63,882,904 (GRCm39)	missense	probably damaging	1.00
R0243:Tnc	UTSW	4	63,888,657 (GRCm39)	missense	probably damaging	0.98
R0362:Tnc	UTSW	4	63,935,679 (GRCm39)	missense	probably damaging	1.00
R0410:Tnc	UTSW	4	63,925,931 (GRCm39)	missense	probably benign	0.00
R0420:Tnc	UTSW	4	63,918,396 (GRCm39)	missense	probably benign	0.00
R0540:Tnc	UTSW	4	63,938,692 (GRCm39)	missense	probably damaging	1.00
R0650:Tnc	UTSW	4	63,926,971 (GRCm39)	missense	probably benign	0.00
R1019:Tnc	UTSW	4	63,880,319 (GRCm39)	missense	probably damaging	1.00
R1102:Tnc	UTSW	4	63,938,705 (GRCm39)	missense	probably benign	0.05
R1126:Tnc	UTSW	4	63,936,357 (GRCm39)	missense	probably damaging	0.99
R1141:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1142:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1307:Tnc	UTSW	4	63,927,096 (GRCm39)	missense	probably damaging	0.98
R1322:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1414:Tnc	UTSW	4	63,883,932 (GRCm39)	splice site	probably benign
R1470:Tnc	UTSW	4	63,884,811 (GRCm39)	missense	probably damaging	1.00
R1470:Tnc	UTSW	4	63,884,811 (GRCm39)	missense	probably damaging	1.00
R1499:Tnc	UTSW	4	63,882,991 (GRCm39)	missense	probably benign	0.15
R1506:Tnc	UTSW	4	63,925,921 (GRCm39)	missense	possibly damaging	0.90
R1597:Tnc	UTSW	4	63,924,621 (GRCm39)	missense	probably benign
R1750:Tnc	UTSW	4	63,890,972 (GRCm39)	missense	probably damaging	1.00
R1765:Tnc	UTSW	4	63,932,231 (GRCm39)	missense	probably damaging	1.00
R1783:Tnc	UTSW	4	63,936,333 (GRCm39)	missense	probably damaging	0.98
R1808:Tnc	UTSW	4	63,918,168 (GRCm39)	missense	probably damaging	1.00
R1903:Tnc	UTSW	4	63,918,299 (GRCm39)	missense	probably benign	0.00
R1932:Tnc	UTSW	4	63,911,262 (GRCm39)	critical splice donor site	probably null
R1941:Tnc	UTSW	4	63,933,201 (GRCm39)	missense	probably damaging	1.00
R1983:Tnc	UTSW	4	63,902,867 (GRCm39)	missense	possibly damaging	0.95
R2024:Tnc	UTSW	4	63,882,858 (GRCm39)	missense	probably damaging	1.00
R2075:Tnc	UTSW	4	63,913,903 (GRCm39)	missense	possibly damaging	0.94
R2327:Tnc	UTSW	4	63,893,475 (GRCm39)	missense	possibly damaging	0.78
R2444:Tnc	UTSW	4	63,933,200 (GRCm39)	missense	probably damaging	1.00
R2982:Tnc	UTSW	4	63,938,756 (GRCm39)	missense	possibly damaging	0.81
R3874:Tnc	UTSW	4	63,926,947 (GRCm39)	missense	probably damaging	1.00
R4110:Tnc	UTSW	4	63,933,188 (GRCm39)	missense	probably damaging	1.00
R4360:Tnc	UTSW	4	63,935,161 (GRCm39)	missense	probably benign	0.35
R4371:Tnc	UTSW	4	63,888,588 (GRCm39)	missense	probably damaging	1.00
R4434:Tnc	UTSW	4	63,926,066 (GRCm39)	missense	possibly damaging	0.91
R4438:Tnc	UTSW	4	63,926,066 (GRCm39)	missense	possibly damaging	0.91
R4570:Tnc	UTSW	4	63,913,909 (GRCm39)	missense	probably damaging	0.99
R4595:Tnc	UTSW	4	63,913,982 (GRCm39)	missense	probably damaging	1.00
R4749:Tnc	UTSW	4	63,913,876 (GRCm39)	missense	possibly damaging	0.56
R4756:Tnc	UTSW	4	63,885,580 (GRCm39)	missense	probably damaging	0.99
R4824:Tnc	UTSW	4	63,935,857 (GRCm39)	nonsense	probably null
R4957:Tnc	UTSW	4	63,894,793 (GRCm39)	missense	probably damaging	1.00
R4977:Tnc	UTSW	4	63,924,485 (GRCm39)	missense	possibly damaging	0.82
R5001:Tnc	UTSW	4	63,918,299 (GRCm39)	missense	probably benign	0.16
R5001:Tnc	UTSW	4	63,902,726 (GRCm39)	missense	probably damaging	1.00
R5015:Tnc	UTSW	4	63,924,739 (GRCm39)	missense	probably damaging	1.00
R5049:Tnc	UTSW	4	63,936,223 (GRCm39)	missense	probably damaging	1.00
R5066:Tnc	UTSW	4	63,893,466 (GRCm39)	missense	probably damaging	0.96
R5073:Tnc	UTSW	4	63,938,648 (GRCm39)	missense	probably damaging	1.00
R5116:Tnc	UTSW	4	63,885,452 (GRCm39)	critical splice donor site	probably null
R5195:Tnc	UTSW	4	63,885,489 (GRCm39)	missense	probably damaging	1.00
R5200:Tnc	UTSW	4	63,889,515 (GRCm39)	missense	probably damaging	1.00
R5221:Tnc	UTSW	4	63,911,534 (GRCm39)	nonsense	probably null
R5237:Tnc	UTSW	4	63,880,333 (GRCm39)	missense	probably damaging	1.00
R5265:Tnc	UTSW	4	63,911,443 (GRCm39)	missense	probably benign	0.00
R5275:Tnc	UTSW	4	63,882,967 (GRCm39)	nonsense	probably null
R5346:Tnc	UTSW	4	63,926,892 (GRCm39)	missense	probably benign
R5409:Tnc	UTSW	4	63,925,654 (GRCm39)	missense	probably damaging	1.00
R5409:Tnc	UTSW	4	63,884,773 (GRCm39)	missense	probably damaging	1.00
R5469:Tnc	UTSW	4	63,932,162 (GRCm39)	splice site	probably null
R5518:Tnc	UTSW	4	63,935,916 (GRCm39)	missense	probably damaging	1.00
R5560:Tnc	UTSW	4	63,926,946 (GRCm39)	missense	probably damaging	1.00
R5588:Tnc	UTSW	4	63,924,659 (GRCm39)	missense	possibly damaging	0.57
R5686:Tnc	UTSW	4	63,925,967 (GRCm39)	splice site	probably null
R5686:Tnc	UTSW	4	63,927,032 (GRCm39)	missense	possibly damaging	0.78
R5837:Tnc	UTSW	4	63,931,451 (GRCm39)	missense	probably damaging	1.00
R5976:Tnc	UTSW	4	63,936,403 (GRCm39)	missense	probably benign	0.17
R6156:Tnc	UTSW	4	63,888,589 (GRCm39)	missense	probably damaging	1.00
R6182:Tnc	UTSW	4	63,927,033 (GRCm39)	missense	probably damaging	0.99
R6416:Tnc	UTSW	4	63,926,053 (GRCm39)	missense	probably benign	0.05
R6778:Tnc	UTSW	4	63,913,835 (GRCm39)	missense	probably benign	0.12
R6798:Tnc	UTSW	4	63,883,841 (GRCm39)	missense	probably benign	0.02
R6799:Tnc	UTSW	4	63,883,841 (GRCm39)	missense	probably benign	0.02
R6943:Tnc	UTSW	4	63,900,982 (GRCm39)	missense	probably damaging	0.97
R7027:Tnc	UTSW	4	63,902,826 (GRCm39)	missense	probably benign	0.02
R7183:Tnc	UTSW	4	63,931,365 (GRCm39)	missense	probably damaging	1.00
R7204:Tnc	UTSW	4	63,889,392 (GRCm39)	splice site	probably null
R7317:Tnc	UTSW	4	63,890,959 (GRCm39)	missense	probably damaging	0.99
R7323:Tnc	UTSW	4	63,889,469 (GRCm39)	missense	probably damaging	0.96
R7327:Tnc	UTSW	4	63,882,999 (GRCm39)	splice site	probably null
R7382:Tnc	UTSW	4	63,932,280 (GRCm39)	nonsense	probably null
R7399:Tnc	UTSW	4	63,938,894 (GRCm39)	start gained	probably benign
R7479:Tnc	UTSW	4	63,935,865 (GRCm39)	missense	possibly damaging	0.95
R7585:Tnc	UTSW	4	63,938,648 (GRCm39)	missense	probably damaging	1.00
R7932:Tnc	UTSW	4	63,926,857 (GRCm39)	missense	probably benign
R7947:Tnc	UTSW	4	63,935,580 (GRCm39)	missense	probably damaging	1.00
R7974:Tnc	UTSW	4	63,918,961 (GRCm39)	missense	possibly damaging	0.84
R7991:Tnc	UTSW	4	63,926,983 (GRCm39)	missense	probably benign	0.42
R8004:Tnc	UTSW	4	63,902,894 (GRCm39)	missense	probably benign	0.04
R8080:Tnc	UTSW	4	63,894,706 (GRCm39)	missense	possibly damaging	0.52
R8109:Tnc	UTSW	4	63,927,000 (GRCm39)	missense	probably benign	0.11
R8145:Tnc	UTSW	4	63,935,716 (GRCm39)	missense	probably benign
R8340:Tnc	UTSW	4	63,926,036 (GRCm39)	missense	probably damaging	1.00
R8360:Tnc	UTSW	4	63,885,511 (GRCm39)	missense	probably benign	0.00
R8671:Tnc	UTSW	4	63,935,683 (GRCm39)	missense	probably damaging	1.00
R8691:Tnc	UTSW	4	63,880,313 (GRCm39)	missense	probably damaging	1.00
R8759:Tnc	UTSW	4	63,924,501 (GRCm39)	missense	possibly damaging	0.86
R8864:Tnc	UTSW	4	63,911,296 (GRCm39)	missense	probably damaging	0.98
R8927:Tnc	UTSW	4	63,925,595 (GRCm39)	missense	probably damaging	1.00
R8928:Tnc	UTSW	4	63,925,595 (GRCm39)	missense	probably damaging	1.00
R8949:Tnc	UTSW	4	63,927,087 (GRCm39)	missense	probably damaging	1.00
R8956:Tnc	UTSW	4	63,918,970 (GRCm39)	missense	probably damaging	1.00
R9016:Tnc	UTSW	4	63,935,331 (GRCm39)	missense	probably benign	0.23
R9049:Tnc	UTSW	4	63,918,247 (GRCm39)	missense	possibly damaging	0.83
R9097:Tnc	UTSW	4	63,888,622 (GRCm39)	missense	possibly damaging	0.62
R9114:Tnc	UTSW	4	63,890,973 (GRCm39)	missense	probably benign	0.03
R9151:Tnc	UTSW	4	63,938,686 (GRCm39)	missense	possibly damaging	0.46
R9488:Tnc	UTSW	4	63,913,942 (GRCm39)	missense	probably damaging	0.99
R9537:Tnc	UTSW	4	63,884,821 (GRCm39)	missense	probably damaging	0.99
R9666:Tnc	UTSW	4	63,926,045 (GRCm39)	missense	probably damaging	1.00
R9700:Tnc	UTSW	4	63,933,186 (GRCm39)	missense	probably damaging	0.99
R9703:Tnc	UTSW	4	63,889,412 (GRCm39)	missense	probably benign	0.00
R9771:Tnc	UTSW	4	63,925,600 (GRCm39)	missense	probably damaging	1.00
S24628:Tnc	UTSW	4	63,936,249 (GRCm39)	missense	probably damaging	1.00
Z1177:Tnc	UTSW	4	63,925,663 (GRCm39)	nonsense	probably null
Z1177:Tnc	UTSW	4	63,878,781 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- TGGCTATCCACCATCAGACC -3'
(R):5'- GTGCCTTCCCTGGAAAATCTCAC -3'

Sequencing Primer
(F):5'- GACCCCCATGTCACTTCAG -3'
(R):5'- AATCTCACTGTGACTGAGGC -3'

Posted On

2018-04-27