Mutagenetix > Incidental Mutation

Incidental Mutation 'R6337:Vipr2'

513769

Institutional Source

Beutler Lab

Gene Symbol

Vipr2

Ensembl Gene

ENSMUSG00000011171

Gene Name

vasoactive intestinal peptide receptor 2

Synonyms

VPAC2R, VPAC2, VIP receptor subtype 2, Vip2

MMRRC Submission

044491-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R6337 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

116041346-116109881 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 116086363 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 129 (Y129*)

Ref Sequence

ENSEMBL: ENSMUSP00000011315 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000011315]

AlphaFold

P41588

Predicted Effect

probably null
Transcript: ENSMUST00000011315
AA Change: Y129*

SMART Domains

Protein: ENSMUSP00000011315
Gene: ENSMUSG00000011171
AA Change: Y129*

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
HormR	47	117	8.35e-25	SMART
Pfam:7tm_2	122	370	1.5e-81	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000175871

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000176078

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000177059

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000177199

Meta Mutation Damage Score

0.9711

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 94.1%

Validation Efficiency

98% (61/62)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase. [provided by RefSeq, Aug 2011]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit enhanced delayed-type hypersensitivity (type IV) and reduced immediate-type hypersensitivity (type I). [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933434E20Rik	T	G	3: 89,969,040 (GRCm39)	V221G	probably benign	Het
Abca12	G	A	1: 71,334,172 (GRCm39)	A1110V	probably damaging	Het
Acsl6	C	A	11: 54,231,368 (GRCm39)	P462T	probably damaging	Het
Acsm5	G	A	7: 119,133,458 (GRCm39)	A208T	probably benign	Het
Adcyap1	T	A	17: 93,509,709 (GRCm39)	Y53*	probably null	Het
Adgrg7	T	A	16: 56,572,788 (GRCm39)	I343F	probably damaging	Het
Agl	T	A	3: 116,580,426 (GRCm39)	K376M	possibly damaging	Het
Akna	A	G	4: 63,292,240 (GRCm39)	Y1142H	probably benign	Het
Anks1b	A	T	10: 90,757,158 (GRCm39)	T182S	probably benign	Het
Apol7e	T	A	15: 77,598,582 (GRCm39)	Y16N	possibly damaging	Het
Bptf	G	T	11: 106,949,605 (GRCm39)	T2238K	possibly damaging	Het
Ccdc87	A	G	19: 4,889,829 (GRCm39)	E107G	probably benign	Het
Ccng2	C	T	5: 93,418,780 (GRCm39)	A135V	probably benign	Het
Chd8	C	A	14: 52,441,566 (GRCm39)	R842L	probably damaging	Het
Cldn18	T	A	9: 99,591,995 (GRCm39)	T3S	probably benign	Het
Dhcr7	T	C	7: 143,390,468 (GRCm39)		probably null	Het
Dnah8	G	A	17: 30,967,542 (GRCm39)	D2585N	probably benign	Het
Dusp26	T	C	8: 31,586,325 (GRCm39)	V182A	probably damaging	Het
Eif3e	T	C	15: 43,115,692 (GRCm39)	D358G	possibly damaging	Het
Epha3	A	T	16: 63,388,806 (GRCm39)	L814H	probably damaging	Het
Flnc	A	G	6: 29,454,318 (GRCm39)	N1877S	probably damaging	Het
Fpgs	A	T	2: 32,577,953 (GRCm39)	Y156*	probably null	Het
Gm10549	C	A	18: 33,597,358 (GRCm39)		probably benign	Het
Gm11595	G	A	11: 99,663,381 (GRCm39)	R100C	unknown	Het
Gm5591	T	C	7: 38,221,319 (GRCm39)	D250G	probably benign	Het
Gtf2e2	C	T	8: 34,266,043 (GRCm39)	R240*	probably null	Het
Hells	G	T	19: 38,943,254 (GRCm39)	Q519H	probably benign	Het
Hscb	A	G	5: 110,987,360 (GRCm39)		probably null	Het
Inpp5k	T	A	11: 75,537,640 (GRCm39)	I350N	probably damaging	Het
Irf9	G	A	14: 55,843,799 (GRCm39)	V221I	possibly damaging	Het
Itga8	A	T	2: 12,258,280 (GRCm39)	Y261*	probably null	Het
Itprid2	G	A	2: 79,485,463 (GRCm39)	D506N	probably damaging	Het
Lama1	C	T	17: 68,093,014 (GRCm39)	T1684M	probably benign	Het
Lrp2	A	T	2: 69,268,811 (GRCm39)	H4157Q	probably damaging	Het
Nceh1	G	T	3: 27,276,956 (GRCm39)	R93L	probably damaging	Het
Nell2	A	G	15: 95,283,025 (GRCm39)	F339S	probably damaging	Het
Or2t49	A	T	11: 58,392,838 (GRCm39)	C181*	probably null	Het
Phf11a	C	A	14: 59,521,817 (GRCm39)	C118F	probably damaging	Het
Purg	A	G	8: 33,876,451 (GRCm39)	K30E	possibly damaging	Het
Qprt	C	T	7: 126,708,101 (GRCm39)	R110H	probably damaging	Het
Robo2	T	C	16: 73,725,039 (GRCm39)	T1055A	probably benign	Het
Serpina3a	T	C	12: 104,079,137 (GRCm39)	F10L	probably benign	Het
Sidt1	A	T	16: 44,121,298 (GRCm39)		probably null	Het
Slc4a4	T	A	5: 89,194,231 (GRCm39)	M237K	probably benign	Het
Slitrk5	A	T	14: 111,917,684 (GRCm39)	D436V	probably damaging	Het
Snd1	T	C	6: 28,888,288 (GRCm39)	Y908H	probably damaging	Het
Sorcs1	A	G	19: 50,132,562 (GRCm39)	V1132A	probably benign	Het
Speer3	A	G	5: 13,843,369 (GRCm39)	E92G	probably damaging	Het
Strada	T	C	11: 106,064,143 (GRCm39)	E58G	possibly damaging	Het
Tbx10	A	T	19: 4,047,312 (GRCm39)	K139*	probably null	Het
Tcf4	T	C	18: 69,766,651 (GRCm39)	Y25H	probably damaging	Het
Tmem108	C	T	9: 103,376,960 (GRCm39)	R163H	possibly damaging	Het
Top2b	A	G	14: 16,399,026 (GRCm38)	T549A	possibly damaging	Het
Tpcn2	A	G	7: 144,833,080 (GRCm39)	I46T	probably damaging	Het
Uox	C	T	3: 146,330,332 (GRCm39)	R163*	probably null	Het
Vps37a	A	T	8: 40,993,749 (GRCm39)	Q248L	probably benign	Het
Wrap53	T	C	11: 69,468,511 (GRCm39)	I179V	probably benign	Het
Zan	G	A	5: 137,450,750 (GRCm39)	A1609V	unknown	Het
Zbtb17	G	A	4: 141,190,694 (GRCm39)	G171S	probably benign	Het
Zbtb5	G	A	4: 44,993,459 (GRCm39)	R642W	probably damaging	Het
Zfyve27	A	G	19: 42,171,096 (GRCm39)		probably null	Het
Zup1	T	C	10: 33,825,252 (GRCm39)	N77D	probably benign	Het

Other mutations in Vipr2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00691:Vipr2	APN	12	116,102,368 (GRCm39)	splice site	probably null
IGL02233:Vipr2	APN	12	116,058,356 (GRCm39)	missense	probably damaging	0.99
IGL02691:Vipr2	APN	12	116,099,849 (GRCm39)	missense	probably benign	0.11
PIT4377001:Vipr2	UTSW	12	116,058,418 (GRCm39)	missense	probably benign	0.01
R0135:Vipr2	UTSW	12	116,106,447 (GRCm39)	missense	probably benign	0.00
R0207:Vipr2	UTSW	12	116,106,502 (GRCm39)	missense	probably damaging	1.00
R1389:Vipr2	UTSW	12	116,100,950 (GRCm39)	missense	probably benign	0.01
R1560:Vipr2	UTSW	12	116,058,401 (GRCm39)	missense	probably benign	0.18
R1575:Vipr2	UTSW	12	116,107,892 (GRCm39)	missense	probably benign
R1696:Vipr2	UTSW	12	116,102,777 (GRCm39)	missense	probably benign	0.13
R1970:Vipr2	UTSW	12	116,099,826 (GRCm39)	missense	probably benign	0.01
R2010:Vipr2	UTSW	12	116,086,430 (GRCm39)	critical splice donor site	probably null
R3873:Vipr2	UTSW	12	116,099,724 (GRCm39)	unclassified	probably benign
R4713:Vipr2	UTSW	12	116,043,751 (GRCm39)	missense	probably benign	0.00
R4953:Vipr2	UTSW	12	116,107,876 (GRCm39)	missense	probably benign	0.07
R6041:Vipr2	UTSW	12	116,106,604 (GRCm39)	missense	probably damaging	1.00
R6902:Vipr2	UTSW	12	116,102,819 (GRCm39)	missense	possibly damaging	0.46
R6946:Vipr2	UTSW	12	116,102,819 (GRCm39)	missense	possibly damaging	0.46
R7763:Vipr2	UTSW	12	116,086,338 (GRCm39)	missense	probably damaging	1.00
R9339:Vipr2	UTSW	12	116,058,344 (GRCm39)	missense	probably damaging	0.96
R9523:Vipr2	UTSW	12	116,093,788 (GRCm39)	missense	probably damaging	1.00
X0066:Vipr2	UTSW	12	116,106,565 (GRCm39)	splice site	probably null
X0067:Vipr2	UTSW	12	116,102,792 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAAATGACCCTGTCTCCTGTC -3'
(R):5'- TCTCTATAGGTTGGTACACAGAGG -3'

Sequencing Primer
(F):5'- GCACTTGGCATGCAAGAG -3'
(R):5'- GGGTCAAGAGTCTAAACCTCTG -3'

Posted On

2018-04-27