Mutagenetix > Incidental Mutation

Incidental Mutation 'R6340:Vmn2r82'

513889

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r82

Ensembl Gene

ENSMUSG00000091468

Gene Name

vomeronasal 2, receptor 82

Synonyms

EG624845

MMRRC Submission

044494-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R6340 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

79192425-79232600 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 79231727 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 575 (R575S)

Ref Sequence

ENSEMBL: ENSMUSP00000130114 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170596]

AlphaFold

G3UWA2

Predicted Effect

probably benign
Transcript: ENSMUST00000170596
AA Change: R575S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000130114
Gene: ENSMUSG00000091468
AA Change: R575S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	79	474	6e-35	PFAM
Pfam:NCD3G	517	570	9.3e-22	PFAM
Pfam:7tm_3	603	838	6.5e-49	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.7%

Validation Efficiency

98% (41/42)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgef4	C	T	1: 34,771,304 (GRCm39)	L1204F	probably damaging	Het
Atp9b	T	C	18: 80,822,115 (GRCm39)	D495G	probably benign	Het
B020011L13Rik	G	A	1: 117,729,614 (GRCm39)	V374I	probably benign	Het
Babam1	T	A	8: 71,855,449 (GRCm39)	V214D	probably damaging	Het
Chpf2	T	C	5: 24,796,773 (GRCm39)	V573A	probably damaging	Het
Elmo3	T	A	8: 106,033,379 (GRCm39)	I115N	probably damaging	Het
Emc1	T	A	4: 139,092,874 (GRCm39)	Y564N	probably damaging	Het
Fam180a	A	C	6: 35,292,322 (GRCm39)	L28R	probably damaging	Het
Fiz1	C	T	7: 5,011,400 (GRCm39)	A373T	possibly damaging	Het
Fnip2	A	G	3: 79,415,152 (GRCm39)	F243L	probably damaging	Het
Gabra5	A	T	7: 57,063,496 (GRCm39)	N309K	probably damaging	Het
Gm17654	A	G	14: 43,815,534 (GRCm39)	L91P	unknown	Het
Gm19684	G	A	17: 36,438,402 (GRCm39)		probably null	Het
Gm9376	A	T	14: 118,504,669 (GRCm39)	M34L	unknown	Het
Hmgcr	A	G	13: 96,802,366 (GRCm39)	F106S	probably damaging	Het
Hydin	T	G	8: 111,081,574 (GRCm39)		probably null	Het
Igf2bp3	A	G	6: 49,191,393 (GRCm39)	Y5H	probably damaging	Het
Igsf11	A	G	16: 38,829,336 (GRCm39)	T77A	probably benign	Het
Il20	A	G	1: 130,836,118 (GRCm39)	Y142H	probably benign	Het
Macf1	A	T	4: 123,342,042 (GRCm39)	D2160E	probably benign	Het
Melk	A	G	4: 44,340,633 (GRCm39)	Y310C	probably damaging	Het
Mep1a	C	T	17: 43,789,949 (GRCm39)	V454M	probably benign	Het
Mep1a	A	T	17: 43,790,124 (GRCm39)	N395K	probably benign	Het
Mnt	A	G	11: 74,727,242 (GRCm39)	K43E	probably damaging	Het
Nrap	A	G	19: 56,335,616 (GRCm39)	I853T	probably damaging	Het
Ogfrl1	T	G	1: 23,408,944 (GRCm39)	K427N	probably benign	Het
Or1j13	T	C	2: 36,370,032 (GRCm39)	T37A	probably benign	Het
Or8d1b	T	G	9: 38,887,062 (GRCm39)	L30R	probably damaging	Het
Pdia4	A	G	6: 47,777,952 (GRCm39)	S347P	probably benign	Het
Pkd1l1	T	G	11: 8,794,649 (GRCm39)	Q1837H	probably benign	Het
Pramel11	T	C	4: 143,623,877 (GRCm39)	N99S	possibly damaging	Het
Pygo1	T	A	9: 72,852,711 (GRCm39)	N299K	probably damaging	Het
Sirpb1b	A	T	3: 15,613,725 (GRCm39)	V52E	probably damaging	Het
Tdrd7	T	A	4: 45,994,517 (GRCm39)	I305N	probably damaging	Het
Trav14-1	T	C	14: 53,791,955 (GRCm39)	S102P	probably damaging	Het
Tsga10	C	A	1: 37,874,266 (GRCm39)		probably benign	Het
Uso1	T	A	5: 92,347,711 (GRCm39)	H837Q	probably benign	Het
Yae1d1	C	A	13: 18,167,861 (GRCm39)	A11S	probably benign	Het
Zfp277	A	G	12: 40,368,548 (GRCm39)	I459T	possibly damaging	Het

Other mutations in Vmn2r82

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01800:Vmn2r82	APN	10	79,192,581 (GRCm39)	missense	probably benign	0.03
IGL01860:Vmn2r82	APN	10	79,214,691 (GRCm39)	missense	probably benign	0.18
IGL01927:Vmn2r82	APN	10	79,213,906 (GRCm39)	missense	probably damaging	1.00
IGL01929:Vmn2r82	APN	10	79,214,545 (GRCm39)	missense	probably damaging	1.00
IGL02028:Vmn2r82	APN	10	79,215,057 (GRCm39)	missense	probably benign
IGL02112:Vmn2r82	APN	10	79,231,833 (GRCm39)	missense	probably benign	0.19
IGL02632:Vmn2r82	APN	10	79,192,542 (GRCm39)	missense	probably benign	0.45
IGL02665:Vmn2r82	APN	10	79,215,205 (GRCm39)	missense	probably damaging	0.99
IGL02716:Vmn2r82	APN	10	79,213,678 (GRCm39)	missense	probably benign	0.20
IGL03030:Vmn2r82	APN	10	79,217,149 (GRCm39)	missense	possibly damaging	0.85
IGL03190:Vmn2r82	APN	10	79,192,643 (GRCm39)	splice site	probably null
IGL03349:Vmn2r82	APN	10	79,213,703 (GRCm39)	missense	probably benign	0.25
IGL03048:Vmn2r82	UTSW	10	79,232,460 (GRCm39)	missense	probably damaging	0.98
R0080:Vmn2r82	UTSW	10	79,232,339 (GRCm39)	missense	probably benign	0.00
R0193:Vmn2r82	UTSW	10	79,217,129 (GRCm39)	missense	probably damaging	1.00
R0217:Vmn2r82	UTSW	10	79,214,634 (GRCm39)	missense	possibly damaging	0.46
R0285:Vmn2r82	UTSW	10	79,232,391 (GRCm39)	missense	probably damaging	1.00
R1193:Vmn2r82	UTSW	10	79,213,739 (GRCm39)	nonsense	probably null
R1385:Vmn2r82	UTSW	10	79,232,325 (GRCm39)	nonsense	probably null
R1386:Vmn2r82	UTSW	10	79,214,545 (GRCm39)	missense	probably damaging	1.00
R1442:Vmn2r82	UTSW	10	79,215,201 (GRCm39)	missense	probably benign	0.03
R1467:Vmn2r82	UTSW	10	79,232,133 (GRCm39)	missense	probably benign	0.00
R1467:Vmn2r82	UTSW	10	79,232,133 (GRCm39)	missense	probably benign	0.00
R1518:Vmn2r82	UTSW	10	79,214,702 (GRCm39)	missense	probably damaging	1.00
R1538:Vmn2r82	UTSW	10	79,192,578 (GRCm39)	missense	possibly damaging	0.92
R1607:Vmn2r82	UTSW	10	79,215,253 (GRCm39)	missense	possibly damaging	0.67
R1812:Vmn2r82	UTSW	10	79,215,046 (GRCm39)	missense	probably benign	0.33
R1906:Vmn2r82	UTSW	10	79,232,344 (GRCm39)	missense	probably damaging	1.00
R1954:Vmn2r82	UTSW	10	79,231,890 (GRCm39)	missense	probably damaging	1.00
R1972:Vmn2r82	UTSW	10	79,214,680 (GRCm39)	missense	probably damaging	1.00
R2093:Vmn2r82	UTSW	10	79,231,813 (GRCm39)	missense	probably benign	0.30
R2156:Vmn2r82	UTSW	10	79,214,722 (GRCm39)	missense	probably damaging	1.00
R2202:Vmn2r82	UTSW	10	79,192,519 (GRCm39)	missense	probably benign
R2442:Vmn2r82	UTSW	10	79,221,210 (GRCm39)	missense	probably damaging	1.00
R2444:Vmn2r82	UTSW	10	79,213,702 (GRCm39)	missense	possibly damaging	0.65
R2857:Vmn2r82	UTSW	10	79,217,090 (GRCm39)	missense	probably damaging	0.98
R2858:Vmn2r82	UTSW	10	79,217,090 (GRCm39)	missense	probably damaging	0.98
R2884:Vmn2r82	UTSW	10	79,232,082 (GRCm39)	missense	probably benign	0.00
R2886:Vmn2r82	UTSW	10	79,232,082 (GRCm39)	missense	probably benign	0.00
R4369:Vmn2r82	UTSW	10	79,231,914 (GRCm39)	missense	probably benign	0.01
R4445:Vmn2r82	UTSW	10	79,214,874 (GRCm39)	missense	possibly damaging	0.87
R4589:Vmn2r82	UTSW	10	79,192,548 (GRCm39)	missense	probably damaging	1.00
R4703:Vmn2r82	UTSW	10	79,214,641 (GRCm39)	missense	probably damaging	1.00
R4908:Vmn2r82	UTSW	10	79,214,589 (GRCm39)	missense	probably benign	0.00
R4937:Vmn2r82	UTSW	10	79,215,010 (GRCm39)	missense	probably benign	0.01
R5199:Vmn2r82	UTSW	10	79,231,921 (GRCm39)	missense	probably damaging	1.00
R5391:Vmn2r82	UTSW	10	79,192,491 (GRCm39)	missense	probably null	0.01
R5601:Vmn2r82	UTSW	10	79,232,025 (GRCm39)	missense	probably damaging	1.00
R5635:Vmn2r82	UTSW	10	79,214,652 (GRCm39)	missense	probably benign	0.33
R6065:Vmn2r82	UTSW	10	79,221,210 (GRCm39)	missense	probably damaging	1.00
R6074:Vmn2r82	UTSW	10	79,232,377 (GRCm39)	missense	probably damaging	1.00
R6474:Vmn2r82	UTSW	10	79,214,871 (GRCm39)	missense	possibly damaging	0.55
R6995:Vmn2r82	UTSW	10	79,232,377 (GRCm39)	missense	probably damaging	1.00
R7111:Vmn2r82	UTSW	10	79,214,605 (GRCm39)	missense	probably benign	0.22
R7212:Vmn2r82	UTSW	10	79,215,268 (GRCm39)	missense	probably benign	0.00
R7335:Vmn2r82	UTSW	10	79,214,722 (GRCm39)	missense	probably damaging	1.00
R7353:Vmn2r82	UTSW	10	79,232,452 (GRCm39)	missense	probably benign	0.11
R7354:Vmn2r82	UTSW	10	79,192,464 (GRCm39)	missense	probably benign	0.00
R7362:Vmn2r82	UTSW	10	79,232,451 (GRCm39)	missense	probably benign	0.00
R7378:Vmn2r82	UTSW	10	79,232,276 (GRCm39)	nonsense	probably null
R7430:Vmn2r82	UTSW	10	79,217,087 (GRCm39)	missense	probably damaging	1.00
R7509:Vmn2r82	UTSW	10	79,231,842 (GRCm39)	missense	possibly damaging	0.82
R7874:Vmn2r82	UTSW	10	79,232,345 (GRCm39)	missense	probably damaging	1.00
R7943:Vmn2r82	UTSW	10	79,232,079 (GRCm39)	missense	possibly damaging	0.74
R8158:Vmn2r82	UTSW	10	79,213,636 (GRCm39)	missense	probably benign	0.12
R8324:Vmn2r82	UTSW	10	79,214,727 (GRCm39)	nonsense	probably null
R8340:Vmn2r82	UTSW	10	79,217,036 (GRCm39)	missense	probably benign	0.00
R8787:Vmn2r82	UTSW	10	79,213,894 (GRCm39)	missense	probably damaging	1.00
R8929:Vmn2r82	UTSW	10	79,232,541 (GRCm39)	missense	probably benign	0.00
R9018:Vmn2r82	UTSW	10	79,232,539 (GRCm39)	missense	probably damaging	1.00
R9399:Vmn2r82	UTSW	10	79,214,768 (GRCm39)	nonsense	probably null
R9517:Vmn2r82	UTSW	10	79,213,641 (GRCm39)	nonsense	probably null
R9587:Vmn2r82	UTSW	10	79,214,936 (GRCm39)	missense	possibly damaging	0.70
R9602:Vmn2r82	UTSW	10	79,214,880 (GRCm39)	missense	probably benign	0.07
Z1088:Vmn2r82	UTSW	10	79,192,456 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79,232,369 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79,192,429 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- GGCTTTAGCAATATGGTTTTCTGCA -3'
(R):5'- TGAGAGTGATGAGCAGTATGTAACT -3'

Sequencing Primer
(F):5'- ACATGTTTCTCCAGAAGGGGC -3'
(R):5'- AACTGAGGGATCTATTATTGGCC -3'

Posted On

2018-04-27