Incidental Mutation 'R6338:Rnf149'
ID514300
Institutional Source Beutler Lab
Gene Symbol Rnf149
Ensembl Gene ENSMUSG00000048234
Gene Namering finger protein 149
Synonyms1600023E10Rik, Greul4
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.177) question?
Stock #R6338 (G1)
Quality Score225.009
Status Not validated
Chromosome1
Chromosomal Location39551296-39577405 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 39560742 bp
ZygosityHeterozygous
Amino Acid Change Cysteine to Serine at position 268 (C268S)
Ref Sequence ENSEMBL: ENSMUSP00000050388 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000062525] [ENSMUST00000195136] [ENSMUST00000195705]
Predicted Effect probably null
Transcript: ENSMUST00000062525
AA Change: C268S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000050388
Gene: ENSMUSG00000048234
AA Change: C268S

DomainStartEndE-ValueType
low complexity region 2 24 N/A INTRINSIC
Pfam:PA 72 169 3.9e-13 PFAM
transmembrane domain 196 218 N/A INTRINSIC
RING 265 305 2.09e-7 SMART
low complexity region 347 362 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000153381
Predicted Effect probably damaging
Transcript: ENSMUST00000195136
AA Change: C111S

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000141667
Gene: ENSMUSG00000048234
AA Change: C111S

DomainStartEndE-ValueType
transmembrane domain 39 61 N/A INTRINSIC
SCOP:d1ldjb_ 74 127 3e-3 SMART
Blast:RING 108 127 9e-7 BLAST
Predicted Effect probably null
Transcript: ENSMUST00000195705
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.7%
  • 10x: 98.1%
  • 20x: 93.8%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 56 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acer3 T C 7: 98,257,715 Y128C probably damaging Het
Adam30 T G 3: 98,161,541 I102S probably damaging Het
Adcy8 A T 15: 64,920,617 D163E possibly damaging Het
Agrn C T 4: 156,170,585 E1614K probably benign Het
Aldh16a1 A T 7: 45,141,961 W107R probably damaging Het
Arfgef2 A G 2: 166,845,570 D238G probably damaging Het
Arhgap11a T C 2: 113,833,725 S738G probably benign Het
Arid5b C A 10: 68,098,561 G504* probably null Het
Carmil3 T C 14: 55,499,849 V763A possibly damaging Het
Cd209e T A 8: 3,849,154 D186V probably damaging Het
Cdh23 T C 10: 60,413,151 D882G probably damaging Het
Cdh4 T C 2: 179,890,812 V689A probably damaging Het
Cntn6 T A 6: 104,726,139 V174E probably damaging Het
Col7a1 C T 9: 108,956,633 T390M unknown Het
Crybg1 T C 10: 43,992,509 D1017G probably damaging Het
Csmd1 T G 8: 15,932,492 K2725T possibly damaging Het
Dnaaf2 T C 12: 69,198,122 E55G probably damaging Het
Fam13a A G 6: 58,953,499 V476A probably damaging Het
Fem1b A T 9: 62,797,011 D322E probably benign Het
Frmpd1 G A 4: 45,274,489 V466I probably benign Het
Gm11639 C T 11: 104,843,208 R2027* probably null Het
Gm20671 A T 5: 32,820,647 D1794E probably damaging Het
Gpatch2 G T 1: 187,225,514 R22L probably damaging Het
Gtf2h1 A G 7: 46,816,456 T450A probably benign Het
Kcnc2 G C 10: 112,271,856 G51R probably benign Het
Krit1 T G 5: 3,836,857 M702R probably benign Het
Krt34 T C 11: 100,038,490 N298S probably benign Het
Lrrcc1 T A 3: 14,547,316 N376K possibly damaging Het
Myo15 A G 11: 60,478,133 E573G probably damaging Het
Olfr1224-ps1 T A 2: 89,156,371 K268I probably damaging Het
Olfr1390 A G 11: 49,340,867 S112G probably benign Het
Olfr366 A T 2: 37,219,822 D111V probably damaging Het
Olfr391-ps A T 11: 73,799,319 L146Q possibly damaging Het
Olfr661 T A 7: 104,688,171 V52E possibly damaging Het
Olfr736 T A 14: 50,393,400 F215I possibly damaging Het
Phf20 A T 2: 156,273,686 Q309L possibly damaging Het
Plcd1 G A 9: 119,074,991 R292C probably damaging Het
Pold3 A G 7: 100,088,105 V342A possibly damaging Het
Polr2a A T 11: 69,739,679 probably null Het
Ptprcap A G 19: 4,156,224 E102G probably benign Het
Rab11fip5 T C 6: 85,341,378 E843G possibly damaging Het
Rai14 T C 15: 10,574,976 D632G probably damaging Het
Slc6a13 T A 6: 121,334,839 F392I probably damaging Het
Slc7a11 C T 3: 50,384,043 probably null Het
Slf1 T A 13: 77,084,462 probably null Het
Stard9 G A 2: 120,697,485 V1408I probably benign Het
Suclg1 T C 6: 73,264,246 I183T probably damaging Het
Syne1 T C 10: 5,255,475 E3497G probably benign Het
Tax1bp1 C T 6: 52,729,376 R121* probably null Het
Tdpoz2 C T 3: 93,652,336 V110I probably benign Het
Ubn2 A G 6: 38,490,714 T788A probably benign Het
Unc13c T A 9: 73,734,447 I1255F probably damaging Het
Usp44 G T 10: 93,846,513 R275I probably damaging Het
Uspl1 A G 5: 149,215,034 N1015D probably benign Het
Wbp2nl G T 15: 82,299,045 W13C possibly damaging Het
Zc3h14 T A 12: 98,758,590 D170E possibly damaging Het
Other mutations in Rnf149
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02586:Rnf149 APN 1 39565215 missense probably benign 0.19
R1316:Rnf149 UTSW 1 39565320 makesense probably null
R2917:Rnf149 UTSW 1 39552483 missense probably benign 0.00
R4690:Rnf149 UTSW 1 39577214 intron probably benign
R5095:Rnf149 UTSW 1 39555656 missense probably benign 0.19
R6084:Rnf149 UTSW 1 39577174 missense probably benign 0.01
Predicted Primers PCR Primer
(F):5'- CAGCAGCTCTTAGTGGTCAC -3'
(R):5'- GGCAGACAGTGGTTTTCTTTCC -3'

Sequencing Primer
(F):5'- AGCTCTTAGTGGTCACCTACAGG -3'
(R):5'- CTGCTAGCGGGAAGAAGGAAATATG -3'
Posted On2018-04-27