Mutagenetix > Incidental Mutation

Incidental Mutation 'R6413:Vmn2r73'

514815

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r73

Ensembl Gene

ENSMUSG00000070458

Gene Name

vomeronasal 2, receptor 73

Synonyms

EG620928

MMRRC Submission

044555-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.141) question?

Stock #

R6413 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85506755-85525146 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 85519544 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 471 (Y471*)

Ref Sequence

ENSEMBL: ENSMUSP00000076687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077478]

AlphaFold

D3Z7M3

Predicted Effect

probably null
Transcript: ENSMUST00000077478
AA Change: Y471*

SMART Domains

Protein: ENSMUSP00000076687
Gene: ENSMUSG00000070458
AA Change: Y471*

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.4e-30	PFAM
Pfam:NCD3G	508	560	5.9e-21	PFAM
Pfam:7tm_3	590	827	4.4e-51	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.1%

Validation Efficiency

100% (37/37)

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110070M22Rik	C	A	13: 119,624,647 (GRCm39)		probably benign	Het
Agtpbp1	A	G	13: 59,647,834 (GRCm39)	V662A	possibly damaging	Het
Ambra1	T	G	2: 91,599,429 (GRCm39)	V183G	possibly damaging	Het
Amdhd2	A	G	17: 24,377,290 (GRCm39)	S208P	probably damaging	Het
Ankdd1a	C	T	9: 65,417,654 (GRCm39)	V133I	probably benign	Het
Ankrd35	A	G	3: 96,592,129 (GRCm39)	K805R	probably damaging	Het
Arhgap28	T	C	17: 68,182,583 (GRCm39)	Q270R	probably benign	Het
Cabp2	G	A	19: 4,135,698 (GRCm39)		probably null	Het
Ccnt1	A	G	15: 98,441,850 (GRCm39)	S473P	probably benign	Het
Cenpt	G	A	8: 106,572,973 (GRCm39)	P373S	possibly damaging	Het
Cyp3a44	T	A	5: 145,731,254 (GRCm39)	D182V	probably damaging	Het
Ddx54	A	G	5: 120,765,127 (GRCm39)	T799A	probably benign	Het
Dtna	T	A	18: 23,755,071 (GRCm39)	D464E	probably damaging	Het
E030025P04Rik	T	C	11: 109,030,425 (GRCm39)	Y154C	unknown	Het
Ephb2	C	A	4: 136,498,433 (GRCm39)	E215D	probably benign	Het
Eya4	T	C	10: 22,992,724 (GRCm39)	D445G	probably damaging	Het
Flg2	A	T	3: 93,127,683 (GRCm39)	L2198F	unknown	Het
Idh2	TCCCAGG	T	7: 79,748,079 (GRCm39)		probably benign	Het
Klhl22	T	C	16: 17,607,181 (GRCm39)	F496L	probably benign	Het
Numa1	A	T	7: 101,639,778 (GRCm39)		probably null	Het
Papola	T	C	12: 105,772,763 (GRCm39)		probably benign	Het
Phldb1	T	C	9: 44,607,440 (GRCm39)	E1255G	probably damaging	Het
Rgs8	A	T	1: 153,568,619 (GRCm39)	D168V	probably damaging	Het
Rlf	A	G	4: 121,004,522 (GRCm39)	V1486A	probably damaging	Het
Rpa2	G	T	4: 132,501,156 (GRCm39)	A118S	probably benign	Het
Rsf1	G	A	7: 97,229,117 (GRCm39)		probably benign	Het
Serpina1f	T	A	12: 103,659,953 (GRCm39)	I110F	probably damaging	Het
Serpini2	T	C	3: 75,166,921 (GRCm39)	Y112C	probably damaging	Het
Sh2b3	A	G	5: 121,966,986 (GRCm39)	Y43H	probably damaging	Het
Shank2	A	G	7: 143,963,955 (GRCm39)	E514G	probably damaging	Het
Slc44a5	T	C	3: 153,963,387 (GRCm39)	F388S	probably benign	Het
Spmip6	A	T	4: 41,505,135 (GRCm39)	V196D	possibly damaging	Het
Tmem176b	T	C	6: 48,815,266 (GRCm39)	N9S	possibly damaging	Het
Trip11	T	A	12: 101,851,790 (GRCm39)	Q758L	probably benign	Het
Trrap	T	A	5: 144,720,856 (GRCm39)	I231N	possibly damaging	Het
Ttn	A	T	2: 76,730,397 (GRCm39)		probably benign	Het
Vmn2r68	T	C	7: 84,870,973 (GRCm39)	N770S	probably damaging	Het
Vmn2r8	T	G	5: 108,949,589 (GRCm39)	E419D	probably benign	Het
Wdfy4	C	A	14: 32,689,604 (GRCm39)	L2818F	probably damaging	Het
Zfhx4	A	T	3: 5,308,205 (GRCm39)	Y477F	probably damaging	Het

Other mutations in Vmn2r73

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r73	APN	7	85,506,795 (GRCm39)	missense
IGL01151:Vmn2r73	APN	7	85,507,086 (GRCm39)	missense	probably damaging	0.99
IGL01408:Vmn2r73	APN	7	85,521,455 (GRCm39)	missense	probably benign	0.45
IGL01505:Vmn2r73	APN	7	85,507,267 (GRCm39)	nonsense	probably null
IGL01731:Vmn2r73	APN	7	85,506,757 (GRCm39)	makesense	probably null
IGL01818:Vmn2r73	APN	7	85,519,109 (GRCm39)	splice site	probably benign
IGL02236:Vmn2r73	APN	7	85,521,902 (GRCm39)	missense	probably benign	0.00
IGL02265:Vmn2r73	APN	7	85,520,847 (GRCm39)	missense	probably benign	0.02
IGL02266:Vmn2r73	APN	7	85,525,007 (GRCm39)	missense	possibly damaging	0.52
IGL02276:Vmn2r73	APN	7	85,518,980 (GRCm39)	critical splice donor site	probably null
IGL02284:Vmn2r73	APN	7	85,507,123 (GRCm39)	missense	possibly damaging	0.92
IGL02380:Vmn2r73	APN	7	85,507,383 (GRCm39)	missense	probably benign	0.00
IGL02466:Vmn2r73	APN	7	85,522,084 (GRCm39)	missense	probably damaging	1.00
IGL03172:Vmn2r73	APN	7	85,507,495 (GRCm39)	missense	probably benign	0.01
IGL03179:Vmn2r73	APN	7	85,519,468 (GRCm39)	missense	probably benign	0.04
G1Funyon:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R0077:Vmn2r73	UTSW	7	85,525,075 (GRCm39)	missense	probably benign	0.00
R0311:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R0413:Vmn2r73	UTSW	7	85,521,087 (GRCm39)	missense	possibly damaging	0.68
R0494:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R1523:Vmn2r73	UTSW	7	85,519,486 (GRCm39)	missense	probably benign	0.02
R1618:Vmn2r73	UTSW	7	85,525,120 (GRCm39)	nonsense	probably null
R1667:Vmn2r73	UTSW	7	85,506,889 (GRCm39)	missense	probably benign	0.00
R1728:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1729:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1747:Vmn2r73	UTSW	7	85,507,375 (GRCm39)	missense	probably damaging	1.00
R1784:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R2152:Vmn2r73	UTSW	7	85,506,936 (GRCm39)	missense	probably benign	0.03
R2395:Vmn2r73	UTSW	7	85,506,975 (GRCm39)	missense	probably damaging	1.00
R2415:Vmn2r73	UTSW	7	85,521,431 (GRCm39)	missense	probably damaging	1.00
R2926:Vmn2r73	UTSW	7	85,520,871 (GRCm39)	missense	probably benign	0.17
R3719:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R3766:Vmn2r73	UTSW	7	85,521,198 (GRCm39)	missense	probably benign
R3838:Vmn2r73	UTSW	7	85,507,258 (GRCm39)	missense	probably benign	0.00
R3890:Vmn2r73	UTSW	7	85,507,144 (GRCm39)	missense	probably benign
R4030:Vmn2r73	UTSW	7	85,521,044 (GRCm39)	missense	possibly damaging	0.92
R4247:Vmn2r73	UTSW	7	85,521,883 (GRCm39)	missense	probably damaging	0.99
R4409:Vmn2r73	UTSW	7	85,520,768 (GRCm39)	missense	probably damaging	0.97
R4427:Vmn2r73	UTSW	7	85,506,981 (GRCm39)	missense	probably damaging	1.00
R4430:Vmn2r73	UTSW	7	85,519,449 (GRCm39)	missense	probably benign
R4552:Vmn2r73	UTSW	7	85,525,055 (GRCm39)	missense	probably benign	0.12
R4779:Vmn2r73	UTSW	7	85,520,923 (GRCm39)	nonsense	probably null
R4906:Vmn2r73	UTSW	7	85,506,829 (GRCm39)	missense	probably damaging	0.99
R4942:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r73	UTSW	7	85,507,155 (GRCm39)	missense	probably damaging	0.98
R5344:Vmn2r73	UTSW	7	85,525,046 (GRCm39)	missense	probably benign	0.00
R5478:Vmn2r73	UTSW	7	85,518,996 (GRCm39)	missense	probably damaging	1.00
R5527:Vmn2r73	UTSW	7	85,507,575 (GRCm39)	missense	possibly damaging	0.65
R5691:Vmn2r73	UTSW	7	85,507,299 (GRCm39)	missense	probably damaging	1.00
R5826:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R6018:Vmn2r73	UTSW	7	85,521,875 (GRCm39)	missense	possibly damaging	0.48
R6082:Vmn2r73	UTSW	7	85,507,429 (GRCm39)	missense	probably benign	0.00
R6111:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R6233:Vmn2r73	UTSW	7	85,519,099 (GRCm39)	missense	probably benign	0.00
R6278:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R6283:Vmn2r73	UTSW	7	85,521,049 (GRCm39)	missense	probably benign	0.00
R6307:Vmn2r73	UTSW	7	85,506,828 (GRCm39)	missense	probably damaging	1.00
R6782:Vmn2r73	UTSW	7	85,519,563 (GRCm39)	missense	probably benign	0.00
R6884:Vmn2r73	UTSW	7	85,507,213 (GRCm39)	missense	probably benign	0.05
R6921:Vmn2r73	UTSW	7	85,507,446 (GRCm39)	missense	probably benign	0.27
R7169:Vmn2r73	UTSW	7	85,507,663 (GRCm39)	nonsense	probably null
R7206:Vmn2r73	UTSW	7	85,522,075 (GRCm39)	missense	probably benign	0.03
R7311:Vmn2r73	UTSW	7	85,521,192 (GRCm39)	missense	possibly damaging	0.80
R7650:Vmn2r73	UTSW	7	85,521,147 (GRCm39)	missense	probably benign
R7811:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R8263:Vmn2r73	UTSW	7	85,507,619 (GRCm39)	missense	probably benign
R8301:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R8341:Vmn2r73	UTSW	7	85,507,128 (GRCm39)	missense	probably benign	0.01
R8500:Vmn2r73	UTSW	7	85,519,638 (GRCm39)	missense	probably benign	0.01
R8673:Vmn2r73	UTSW	7	85,521,902 (GRCm39)	missense	probably benign	0.01
R8719:Vmn2r73	UTSW	7	85,521,095 (GRCm39)	missense	probably damaging	0.96
R8783:Vmn2r73	UTSW	7	85,507,668 (GRCm39)	missense	probably damaging	0.96
R8828:Vmn2r73	UTSW	7	85,521,179 (GRCm39)	missense	probably damaging	0.98
R8860:Vmn2r73	UTSW	7	85,522,149 (GRCm39)	splice site	probably benign
R9002:Vmn2r73	UTSW	7	85,507,284 (GRCm39)	missense	probably benign	0.32
R9008:Vmn2r73	UTSW	7	85,521,904 (GRCm39)	missense	probably damaging	0.96
R9159:Vmn2r73	UTSW	7	85,521,931 (GRCm39)	missense	possibly damaging	0.90
R9180:Vmn2r73	UTSW	7	85,507,123 (GRCm39)	missense	probably benign	0.07
R9198:Vmn2r73	UTSW	7	85,518,987 (GRCm39)	missense	probably benign	0.01
R9329:Vmn2r73	UTSW	7	85,519,073 (GRCm39)	missense	probably benign
R9448:Vmn2r73	UTSW	7	85,522,027 (GRCm39)	missense	probably benign	0.00
R9463:Vmn2r73	UTSW	7	85,506,795 (GRCm39)	missense
R9589:Vmn2r73	UTSW	7	85,519,659 (GRCm39)	missense	probably benign	0.03
X0023:Vmn2r73	UTSW	7	85,507,664 (GRCm39)	missense	probably benign	0.31
Z1176:Vmn2r73	UTSW	7	85,521,176 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r73	UTSW	7	85,521,480 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GATGTCTAGGGCAGTTTATAGTAAGTC -3'
(R):5'- TACCTACTATTCTAAAGTGGAAGCC -3'

Sequencing Primer
(F):5'- CCAAGTATTTATTCCATGGCAAAGC -3'
(R):5'- CTATTCTAAAGTGGAAGCCTAGTTG -3'

Posted On

2018-05-04