Mutagenetix > Incidental Mutation

Incidental Mutation 'R6378:Ganc'

515101

Institutional Source

Beutler Lab

Gene Symbol

Ganc

Ensembl Gene

ENSMUSG00000062646

Gene Name

glucosidase, alpha; neutral C

Synonyms

5830445O15Rik

MMRRC Submission

044528-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.779) question?

Stock #

R6378 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

120234377-120291347 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 120264307 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 420 (M420T)

Ref Sequence

ENSEMBL: ENSMUSP00000116898 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000135074]

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000043755

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000131523

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132835

Predicted Effect

probably damaging
Transcript: ENSMUST00000135074
AA Change: M420T

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000116898
Gene: ENSMUSG00000062646
AA Change: M420T

Domain	Start	End	E-Value	Type
low complexity region	30	44	N/A	INTRINSIC
Pfam:Gal_mutarotas_2	221	292	2.3e-21	PFAM
Pfam:Glyco_hydro_31	333	778	2.5e-137	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136993

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145134

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152437

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.8%

Validation Efficiency

99% (80/81)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Glycosyl hydrolase enzymes hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. This gene encodes a member of glycosyl hydrolases family 31. This enzyme hydrolyses terminal, non-reducing 1,4-linked alpha-D-glucose residues and releases alpha-D-glucose. This is a key enzyme in glycogen metabolism and its gene localizes to a chromosomal region (15q15) that is associated with susceptibility to diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2014]

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ago2	A	T	15: 72,995,774 (GRCm39)	D408E	probably benign	Het
Agpat2	T	C	2: 26,486,147 (GRCm39)	N178S	probably benign	Het
Arsi	T	C	18: 61,049,573 (GRCm39)	F152S	probably damaging	Het
Atp13a2	T	A	4: 140,734,367 (GRCm39)	L1163Q	probably benign	Het
Bpifb2	T	A	2: 153,733,072 (GRCm39)	L385Q	possibly damaging	Het
C330018D20Rik	A	G	18: 57,095,579 (GRCm39)	L2P	probably damaging	Het
Cby3	A	G	11: 50,250,360 (GRCm39)	T189A	probably damaging	Het
Cdc42bpa	A	T	1: 179,921,561 (GRCm39)	D567V	possibly damaging	Het
Cdh5	T	A	8: 104,853,168 (GRCm39)		probably null	Het
Cela1	C	T	15: 100,585,071 (GRCm39)	V20I	probably benign	Het
Cmpk2	G	A	12: 26,519,415 (GRCm39)	G22E	possibly damaging	Het
Ctcf	T	A	8: 106,390,423 (GRCm39)	V10E	possibly damaging	Het
Dpp10	C	A	1: 123,339,468 (GRCm39)	C353F	probably damaging	Het
Efcab3	A	G	11: 104,999,620 (GRCm39)	S5546G	possibly damaging	Het
Elp3	G	T	14: 65,830,420 (GRCm39)	Y10*	probably null	Het
Eml2	G	A	7: 18,935,088 (GRCm39)	V432I	probably damaging	Het
Eml4	T	A	17: 83,755,646 (GRCm39)	W336R	probably damaging	Het
Erich6	T	C	3: 58,529,780 (GRCm39)		probably null	Het
Eya1	T	C	1: 14,373,027 (GRCm39)	N31D	possibly damaging	Het
Fam81a	T	A	9: 70,017,628 (GRCm39)	N106Y	probably damaging	Het
Frrs1	A	G	3: 116,694,639 (GRCm39)	T487A	possibly damaging	Het
Gimap7	A	T	6: 48,701,116 (GRCm39)	E234V	probably damaging	Het
Gpbp1	T	C	13: 111,570,146 (GRCm39)	N400S	probably damaging	Het
Gucy2g	T	C	19: 55,229,377 (GRCm39)	S98G	probably benign	Het
Hoxd10	T	C	2: 74,524,678 (GRCm39)	I330T	possibly damaging	Het
Ik	T	A	18: 36,890,341 (GRCm39)	I539N	probably damaging	Het
Il17rb	G	A	14: 29,722,320 (GRCm39)	T237I	probably damaging	Het
Ing2	T	A	8: 48,122,293 (GRCm39)	Q85L	probably benign	Het
Lrp4	C	A	2: 91,324,174 (GRCm39)	N1208K	probably benign	Het
Lvrn	T	A	18: 47,028,024 (GRCm39)	S888R	probably benign	Het
Map2	T	C	1: 66,454,488 (GRCm39)	V1126A	probably damaging	Het
Mapkapk5	A	G	5: 121,677,233 (GRCm39)		probably null	Het
Mis18bp1	T	C	12: 65,196,021 (GRCm39)	D581G	probably benign	Het
Muc4	T	C	16: 32,599,320 (GRCm39)	V3289A	probably benign	Het
Myom2	T	A	8: 15,149,356 (GRCm39)	I609N	probably benign	Het
Nav1	A	T	1: 135,382,433 (GRCm39)	M1343K	probably damaging	Het
Ndufaf1	T	C	2: 119,486,207 (GRCm39)	I302V	probably damaging	Het
Neb	T	A	2: 52,183,733 (GRCm39)	K978N	probably damaging	Het
Nol8	A	G	13: 49,820,831 (GRCm39)	E878G	probably damaging	Het
Nrde2	A	T	12: 100,097,016 (GRCm39)	I928N	probably damaging	Het
Nxf1	T	A	19: 8,741,910 (GRCm39)	D145E	probably benign	Het
Obox3	T	A	7: 15,360,027 (GRCm39)	H214L	probably benign	Het
Obscn	T	C	11: 58,964,572 (GRCm39)	E3199G	probably damaging	Het
Ogfod3	T	C	11: 121,093,761 (GRCm39)	E83G	probably benign	Het
Or11a4	T	A	17: 37,536,688 (GRCm39)	V224E	probably benign	Het
Or2r3	T	G	6: 42,448,687 (GRCm39)	M142L	probably benign	Het
Or6b2	A	G	1: 92,408,178 (GRCm39)	L55P	probably damaging	Het
Or8s8	T	C	15: 98,354,425 (GRCm39)	V78A	probably benign	Het
Pcsk4	G	T	10: 80,164,809 (GRCm39)	H69N	probably benign	Het
Plcl2	C	T	17: 50,975,188 (GRCm39)		probably null	Het
Pmfbp1	T	C	8: 110,256,898 (GRCm39)	I534T	probably damaging	Het
Prss23	A	T	7: 89,159,241 (GRCm39)	I276N	probably damaging	Het
Ramac	A	G	7: 81,417,387 (GRCm39)	Y29C	probably damaging	Het
Rhd	T	A	4: 134,621,696 (GRCm39)	F403Y	possibly damaging	Het
Rsf1	CG	CGACGGCGGAG	7: 97,229,115 (GRCm39)		probably benign	Homo
Scg5	A	G	2: 113,657,737 (GRCm39)	V58A	possibly damaging	Het
Scn5a	C	T	9: 119,315,102 (GRCm39)	G1868R	probably damaging	Het
Secisbp2l	A	G	2: 125,610,245 (GRCm39)	S225P	possibly damaging	Het
Sema4f	A	T	6: 82,894,613 (GRCm39)	L486*	probably null	Het
Slc25a47	G	A	12: 108,822,069 (GRCm39)	R286H	probably damaging	Het
Slc5a8	A	C	10: 88,740,916 (GRCm39)	K277T	probably damaging	Het
Slc66a3	T	C	12: 17,047,644 (GRCm39)	Y96C	probably damaging	Het
Sorcs1	T	A	19: 50,213,615 (GRCm39)	E704V	possibly damaging	Het
Sptan1	T	C	2: 29,908,527 (GRCm39)	S1768P	probably damaging	Het
Srd5a2	C	T	17: 74,328,378 (GRCm39)		probably null	Het
Sytl2	A	T	7: 90,007,432 (GRCm39)	K65*	probably null	Het
Tas2r109	T	G	6: 132,957,844 (GRCm39)	I29L	probably benign	Het
Tfap2a	A	T	13: 40,876,717 (GRCm39)	V234E	possibly damaging	Het
Tgfbr3	G	A	5: 107,325,679 (GRCm39)	L128F	probably benign	Het
Trappc12	A	T	12: 28,797,082 (GRCm39)	L150Q	probably damaging	Het
Trim43b	T	A	9: 88,967,452 (GRCm39)	I395L	probably benign	Het
U2surp	C	T	9: 95,373,474 (GRCm39)	E232K	probably benign	Het
Vax1	T	C	19: 59,154,656 (GRCm39)	N327S	probably benign	Het
Vmn1r14	T	C	6: 57,210,587 (GRCm39)	V11A	probably benign	Het
Vmn1r60	A	G	7: 5,547,782 (GRCm39)	V106A	probably damaging	Het
Vmn2r106	T	C	17: 20,498,667 (GRCm39)	S415G	probably benign	Het
Vmn2r3	C	T	3: 64,182,517 (GRCm39)	G394D	probably damaging	Het
Ybx2	A	G	11: 69,831,179 (GRCm39)	E63G	possibly damaging	Het
Zfhx4	T	A	3: 5,308,410 (GRCm39)	N545K	probably benign	Het
Zp1	T	C	19: 10,892,217 (GRCm39)	T56A	probably benign	Het

Other mutations in Ganc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00785:Ganc	APN	2	120,272,079 (GRCm39)	missense	probably damaging	1.00
IGL00913:Ganc	APN	2	120,269,933 (GRCm39)	splice site	probably benign
IGL01077:Ganc	APN	2	120,276,996 (GRCm39)	missense	possibly damaging	0.50
IGL01773:Ganc	APN	2	120,290,365 (GRCm39)	missense	possibly damaging	0.87
IGL01812:Ganc	APN	2	120,242,007 (GRCm39)	missense	probably benign	0.00
IGL02029:Ganc	APN	2	120,290,338 (GRCm39)	missense	probably benign	0.00
IGL02067:Ganc	APN	2	120,236,785 (GRCm39)	missense	probably benign	0.16
IGL02290:Ganc	APN	2	120,278,904 (GRCm39)	missense	possibly damaging	0.90
IGL02355:Ganc	APN	2	120,264,238 (GRCm39)	missense	probably damaging	1.00
IGL02362:Ganc	APN	2	120,264,238 (GRCm39)	missense	probably damaging	1.00
IGL02553:Ganc	APN	2	120,288,615 (GRCm39)	missense	probably benign
IGL02808:Ganc	APN	2	120,241,992 (GRCm39)	missense	probably benign	0.00
IGL02966:Ganc	APN	2	120,264,129 (GRCm39)	missense	probably damaging	1.00
IGL03356:Ganc	APN	2	120,265,769 (GRCm39)	missense	probably benign	0.22
IGL03405:Ganc	APN	2	120,264,247 (GRCm39)	missense	probably damaging	1.00
ingenuous	UTSW	2	120,274,630 (GRCm39)	missense	probably damaging	1.00
R0464:Ganc	UTSW	2	120,267,175 (GRCm39)	missense	probably benign	0.07
R0511:Ganc	UTSW	2	120,278,882 (GRCm39)	nonsense	probably null
R0932:Ganc	UTSW	2	120,288,610 (GRCm39)	missense	probably damaging	0.99
R1467:Ganc	UTSW	2	120,261,409 (GRCm39)	splice site	probably benign
R1902:Ganc	UTSW	2	120,276,963 (GRCm39)	missense	probably damaging	1.00
R2087:Ganc	UTSW	2	120,287,738 (GRCm39)	missense	probably damaging	1.00
R4668:Ganc	UTSW	2	120,261,548 (GRCm39)	missense	probably benign	0.02
R4669:Ganc	UTSW	2	120,261,548 (GRCm39)	missense	probably benign	0.02
R4725:Ganc	UTSW	2	120,265,754 (GRCm39)	missense	probably damaging	0.99
R4735:Ganc	UTSW	2	120,267,104 (GRCm39)	splice site	silent
R4738:Ganc	UTSW	2	120,283,075 (GRCm39)	missense	probably damaging	0.97
R4839:Ganc	UTSW	2	120,290,304 (GRCm39)	missense	probably benign
R4951:Ganc	UTSW	2	120,286,528 (GRCm39)	missense	probably benign	0.00
R5841:Ganc	UTSW	2	120,242,020 (GRCm39)	missense	possibly damaging	0.65
R5997:Ganc	UTSW	2	120,261,086 (GRCm39)	missense	possibly damaging	0.55
R6142:Ganc	UTSW	2	120,261,218 (GRCm39)	critical splice donor site	probably null
R6711:Ganc	UTSW	2	120,281,320 (GRCm39)	missense	possibly damaging	0.74
R6777:Ganc	UTSW	2	120,274,630 (GRCm39)	missense	probably damaging	1.00
R7229:Ganc	UTSW	2	120,258,256 (GRCm39)	missense	possibly damaging	0.92
R7235:Ganc	UTSW	2	120,264,198 (GRCm39)	missense	probably damaging	1.00
R7241:Ganc	UTSW	2	120,272,010 (GRCm39)	missense	probably damaging	1.00
R7326:Ganc	UTSW	2	120,261,080 (GRCm39)	missense	probably damaging	1.00
R7567:Ganc	UTSW	2	120,286,582 (GRCm39)	missense	probably benign	0.01
R7685:Ganc	UTSW	2	120,264,273 (GRCm39)	missense	probably damaging	1.00
R7736:Ganc	UTSW	2	120,264,295 (GRCm39)	missense	possibly damaging	0.83
R7784:Ganc	UTSW	2	120,267,149 (GRCm39)	nonsense	probably null
R7955:Ganc	UTSW	2	120,261,181 (GRCm39)	missense	probably damaging	1.00
R8222:Ganc	UTSW	2	120,276,933 (GRCm39)	missense	probably damaging	1.00
R8247:Ganc	UTSW	2	120,267,181 (GRCm39)	missense	probably null	0.52
R8306:Ganc	UTSW	2	120,252,560 (GRCm39)	missense	probably benign	0.02
R9282:Ganc	UTSW	2	120,290,381 (GRCm39)	missense	probably benign
X0027:Ganc	UTSW	2	120,278,931 (GRCm39)	missense	probably damaging	1.00
Z1177:Ganc	UTSW	2	120,264,275 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CATCTCTTTTAGGCACACAAGC -3'
(R):5'- GCATCTTGAACATGGAAAGGTC -3'

Sequencing Primer
(F):5'- GCACACAAGCCATGCCC -3'
(R):5'- CTGGCTGCTCTTAAAGAAACCTGG -3'

Posted On

2018-05-04