Incidental Mutation 'R6480:Stk35'
ID 517019
Institutional Source Beutler Lab
Gene Symbol Stk35
Ensembl Gene ENSMUSG00000037885
Gene Name serine/threonine kinase 35
Synonyms CLP-36 interacting kinase, CLIK1, 1700054C12Rik
MMRRC Submission 044612-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6480 (G1)
Quality Score 225.009
Status Validated
Chromosome 2
Chromosomal Location 129642437-129674207 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to A at 129652607 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glutamic Acid at position 369 (D369E)
Ref Sequence ENSEMBL: ENSMUSP00000126541 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000165413] [ENSMUST00000166282]
AlphaFold Q80ZW0
Predicted Effect possibly damaging
Transcript: ENSMUST00000165413
AA Change: D369E

PolyPhen 2 Score 0.953 (Sensitivity: 0.79; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000126541
Gene: ENSMUSG00000037885
AA Change: D369E

DomainStartEndE-ValueType
low complexity region 51 69 N/A INTRINSIC
low complexity region 115 137 N/A INTRINSIC
low complexity region 190 206 N/A INTRINSIC
Pfam:Pkinase_Tyr 207 295 2e-6 PFAM
Pfam:Pkinase 207 531 8.6e-51 PFAM
Pfam:Pkinase_Tyr 304 532 2.1e-23 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000166282
SMART Domains Protein: ENSMUSP00000132862
Gene: ENSMUSG00000037885

DomainStartEndE-ValueType
low complexity region 51 69 N/A INTRINSIC
low complexity region 115 137 N/A INTRINSIC
low complexity region 190 206 N/A INTRINSIC
Pfam:Pkinase_Tyr 207 300 5.9e-7 PFAM
Pfam:Pkinase 207 311 1e-9 PFAM
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.8%
  • 20x: 93.4%
Validation Efficiency 98% (57/58)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 59 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Arl8b T A 6: 108,792,010 (GRCm39) N90K possibly damaging Het
Art3 T C 5: 92,540,676 (GRCm39) F140L probably damaging Het
Ascc3 T A 10: 50,587,049 (GRCm39) M967K probably damaging Het
Ccne1 G A 7: 37,806,279 (GRCm39) probably benign Het
Cdc14b T C 13: 64,373,464 (GRCm39) probably null Het
Ceacam2 T A 7: 25,219,414 (GRCm39) E282V probably damaging Het
Clk4 T A 11: 51,161,373 (GRCm39) C86* probably null Het
Cul2 A C 18: 3,417,561 (GRCm39) K115T possibly damaging Het
Dhx29 A T 13: 113,090,322 (GRCm39) K800* probably null Het
Dlec1 T C 9: 118,976,758 (GRCm39) F1741S probably benign Het
Dnah12 A G 14: 26,594,412 (GRCm39) T3455A probably damaging Het
Dnah8 G A 17: 30,967,542 (GRCm39) D2585N probably benign Het
Drd4 A T 7: 140,874,706 (GRCm39) I366F possibly damaging Het
Fam169b T A 7: 68,003,466 (GRCm39) Y273* probably null Het
Fbn1 T A 2: 125,177,338 (GRCm39) Y1833F probably benign Het
Fsip2 A G 2: 82,820,430 (GRCm39) T5388A possibly damaging Het
Gjb6 T C 14: 57,361,899 (GRCm39) I121V probably benign Het
Glg1 T C 8: 111,924,338 (GRCm39) T217A possibly damaging Het
Igkv5-48 A G 6: 69,703,810 (GRCm39) S32P probably benign Het
Itgax C A 7: 127,747,771 (GRCm39) F1062L probably benign Het
Lct G A 1: 128,222,057 (GRCm39) T1494I probably damaging Het
Lilra6 G A 7: 3,915,932 (GRCm39) T309I probably damaging Het
Mical3 T C 6: 121,011,236 (GRCm39) T321A possibly damaging Het
Mmp23 T A 4: 155,736,798 (GRCm39) N104I probably damaging Het
Muc17 T C 5: 137,171,238 (GRCm39) Y131C Het
Naip2 A C 13: 100,298,549 (GRCm39) S496A probably benign Het
Ndufs2 A G 1: 171,064,267 (GRCm39) S348P probably damaging Het
Nepro T C 16: 44,547,438 (GRCm39) S52P probably damaging Het
Nlrp6 A G 7: 140,507,356 (GRCm39) E874G possibly damaging Het
Nsmce1 A G 7: 125,090,590 (GRCm39) V9A probably benign Het
Or1q1 T C 2: 36,887,007 (GRCm39) F62L probably benign Het
Or2a7 T C 6: 43,151,000 (GRCm39) F27L probably benign Het
Or5b105 T A 19: 13,079,838 (GRCm39) T277S probably benign Het
Or6c3 A T 10: 129,308,590 (GRCm39) I10F possibly damaging Het
Per2 A T 1: 91,357,104 (GRCm39) probably null Het
Pgbd1 T C 13: 21,607,646 (GRCm39) I183V probably benign Het
Pik3c2g T C 6: 139,676,195 (GRCm39) V113A probably benign Het
Pipox A G 11: 77,773,474 (GRCm39) L259P probably damaging Het
Pkd1l3 C T 8: 110,365,019 (GRCm39) Q1124* probably null Het
Plcd3 A G 11: 102,965,757 (GRCm39) S492P possibly damaging Het
Rcor2 T G 19: 7,248,411 (GRCm39) M142R probably benign Het
Rwdd3 T C 3: 120,950,101 (GRCm39) E115G probably damaging Het
Slc13a3 T C 2: 165,250,818 (GRCm39) Y475C probably damaging Het
Slc14a2 A T 18: 78,202,297 (GRCm39) I611N possibly damaging Het
Slc25a24 G T 3: 109,043,617 (GRCm39) M91I probably damaging Het
Spg11 T C 2: 121,922,786 (GRCm39) S888G probably benign Het
Spta1 A T 1: 174,014,714 (GRCm39) probably null Het
Tanc1 T C 2: 59,637,986 (GRCm39) S889P probably damaging Het
Tbc1d10b T C 7: 126,798,050 (GRCm39) N697S probably damaging Het
Tcof1 A T 18: 60,947,852 (GRCm39) probably null Het
Tg T A 15: 66,543,160 (GRCm39) Y25N probably damaging Het
Thbs1 T C 2: 117,949,598 (GRCm39) C563R probably damaging Het
Tlr11 A G 14: 50,600,512 (GRCm39) S833G possibly damaging Het
Uggt2 T A 14: 119,294,976 (GRCm39) H550L probably benign Het
Utp20 A G 10: 88,591,048 (GRCm39) probably null Het
Wbp1l T A 19: 46,642,758 (GRCm39) L253Q probably damaging Het
Zfp433 T A 10: 81,556,078 (GRCm39) S161R possibly damaging Het
Zfp865 A G 7: 5,032,782 (GRCm39) T256A probably damaging Het
Zpr1 A G 9: 46,186,009 (GRCm39) D160G probably benign Het
Other mutations in Stk35
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00850:Stk35 APN 2 129,643,912 (GRCm39) missense probably damaging 1.00
IGL02609:Stk35 APN 2 129,643,721 (GRCm39) missense probably damaging 1.00
fingernails UTSW 2 129,652,855 (GRCm39) missense probably damaging 1.00
R2256_Stk35_331 UTSW 2 129,652,427 (GRCm39) nonsense probably null
skinned UTSW 2 129,653,155 (GRCm39) intron probably benign
R0045:Stk35 UTSW 2 129,642,488 (GRCm39) nonsense probably null
R0306:Stk35 UTSW 2 129,643,683 (GRCm39) nonsense probably null
R0784:Stk35 UTSW 2 129,652,722 (GRCm39) nonsense probably null
R1536:Stk35 UTSW 2 129,653,155 (GRCm39) intron probably benign
R2256:Stk35 UTSW 2 129,652,427 (GRCm39) nonsense probably null
R2507:Stk35 UTSW 2 129,643,435 (GRCm39) missense probably damaging 0.97
R2508:Stk35 UTSW 2 129,643,435 (GRCm39) missense probably damaging 0.97
R3848:Stk35 UTSW 2 129,642,656 (GRCm39) missense probably benign 0.13
R3872:Stk35 UTSW 2 129,652,495 (GRCm39) missense possibly damaging 0.82
R4466:Stk35 UTSW 2 129,643,436 (GRCm39) missense probably damaging 0.99
R5144:Stk35 UTSW 2 129,652,855 (GRCm39) missense probably damaging 1.00
R6267:Stk35 UTSW 2 129,652,808 (GRCm39) nonsense probably null
R6296:Stk35 UTSW 2 129,652,808 (GRCm39) nonsense probably null
R6807:Stk35 UTSW 2 129,643,573 (GRCm39) missense probably damaging 0.97
R7203:Stk35 UTSW 2 129,643,513 (GRCm39) missense probably benign
R7476:Stk35 UTSW 2 129,652,645 (GRCm39) missense probably damaging 1.00
R8505:Stk35 UTSW 2 129,643,649 (GRCm39) missense probably damaging 0.99
R8998:Stk35 UTSW 2 129,652,509 (GRCm39) missense probably damaging 1.00
R8999:Stk35 UTSW 2 129,652,509 (GRCm39) missense probably damaging 1.00
R9224:Stk35 UTSW 2 129,652,491 (GRCm39) missense probably damaging 1.00
R9760:Stk35 UTSW 2 129,642,605 (GRCm39) missense probably benign
Predicted Primers PCR Primer
(F):5'- TATGCTGAGGAGCCCTGCTATC -3'
(R):5'- TGTCCGCCTTGGCTGTATAG -3'

Sequencing Primer
(F):5'- AGGAGCCCTGCTATCTCTGG -3'
(R):5'- CTTCGGGAGCCATGTAGAAGTC -3'
Posted On 2018-05-21