Mutagenetix > Incidental Mutation

Incidental Mutation 'R6459:Prg4'

517496

Institutional Source

Beutler Lab

Gene Symbol

Prg4

Ensembl Gene

ENSMUSG00000006014

Gene Name

proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)

Synonyms

MSF, SZP, lubricin, DOL54

MMRRC Submission

044594-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.125) question?

Stock #

R6459 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

150325163-150341916 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

T to C at 150330052 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000125551 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006171] [ENSMUST00000111901] [ENSMUST00000111902] [ENSMUST00000119161] [ENSMUST00000124484] [ENSMUST00000124973] [ENSMUST00000161611] [ENSMUST00000164600] [ENSMUST00000159035] [ENSMUST00000161320] [ENSMUST00000162367]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000006171

SMART Domains

Protein: ENSMUSP00000006171
Gene: ENSMUSG00000006014

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	26	68	9.21e-18	SMART
SO	69	108	4.21e-12	SMART
low complexity region	110	124	N/A	INTRINSIC
HX	169	211	1.67e-7	SMART
HX	213	256	3.76e-10	SMART
Blast:HX	369	422	2e-27	BLAST

Predicted Effect

unknown
Transcript: ENSMUST00000111901
AA Change: T618A

SMART Domains

Protein: ENSMUSP00000107532
Gene: ENSMUSG00000006014
AA Change: T618A

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	26	68	9.21e-18	SMART
SO	69	108	4.21e-12	SMART
low complexity region	110	124	N/A	INTRINSIC
low complexity region	141	151	N/A	INTRINSIC
low complexity region	189	221	N/A	INTRINSIC
low complexity region	241	535	N/A	INTRINSIC
low complexity region	570	623	N/A	INTRINSIC
HX	711	753	1.67e-7	SMART
HX	755	798	3.76e-10	SMART
Blast:HX	911	964	5e-27	BLAST

Predicted Effect

unknown
Transcript: ENSMUST00000111902
AA Change: T665A

SMART Domains

Protein: ENSMUSP00000107533
Gene: ENSMUSG00000006014
AA Change: T665A

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	25	67	2.71e-15	SMART
low complexity region	69	81	N/A	INTRINSIC
low complexity region	86	143	N/A	INTRINSIC
low complexity region	150	171	N/A	INTRINSIC
low complexity region	188	198	N/A	INTRINSIC
low complexity region	236	268	N/A	INTRINSIC
low complexity region	288	582	N/A	INTRINSIC
internal_repeat_1	588	614	2.72e-5	PROSPERO
low complexity region	617	670	N/A	INTRINSIC
internal_repeat_1	680	706	2.72e-5	PROSPERO
HX	758	800	1.67e-7	SMART
HX	802	845	3.76e-10	SMART
Blast:HX	958	1011	4e-27	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000119161

SMART Domains

Protein: ENSMUSP00000112606
Gene: ENSMUSG00000006005

Domain	Start	End	E-Value	Type
coiled coil region	49	370	N/A	INTRINSIC
coiled coil region	423	515	N/A	INTRINSIC
low complexity region	518	534	N/A	INTRINSIC
coiled coil region	539	604	N/A	INTRINSIC
low complexity region	690	703	N/A	INTRINSIC
low complexity region	782	795	N/A	INTRINSIC
low complexity region	811	826	N/A	INTRINSIC
low complexity region	1003	1019	N/A	INTRINSIC
Pfam:TPR_MLP1_2	1036	1167	9.1e-33	PFAM
coiled coil region	1215	1421	N/A	INTRINSIC
coiled coil region	1473	1629	N/A	INTRINSIC
internal_repeat_3	1630	1691	1.48e-5	PROSPERO
low complexity region	1695	1717	N/A	INTRINSIC
low complexity region	1761	1777	N/A	INTRINSIC
internal_repeat_5	1814	1827	5.58e-5	PROSPERO
internal_repeat_3	1819	1881	1.48e-5	PROSPERO
internal_repeat_4	1875	1895	5.58e-5	PROSPERO
internal_repeat_1	1893	1919	2.03e-6	PROSPERO
low complexity region	1920	1933	N/A	INTRINSIC
low complexity region	1942	1981	N/A	INTRINSIC
low complexity region	1989	2014	N/A	INTRINSIC
internal_repeat_4	2017	2036	5.58e-5	PROSPERO
low complexity region	2059	2078	N/A	INTRINSIC
internal_repeat_2	2084	2135	3.95e-6	PROSPERO
internal_repeat_5	2127	2140	5.58e-5	PROSPERO
internal_repeat_1	2154	2179	2.03e-6	PROSPERO
internal_repeat_2	2156	2212	3.95e-6	PROSPERO
low complexity region	2239	2251	N/A	INTRINSIC
low complexity region	2263	2277	N/A	INTRINSIC
low complexity region	2292	2314	N/A	INTRINSIC
low complexity region	2346	2357	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000124484

SMART Domains

Protein: ENSMUSP00000121991
Gene: ENSMUSG00000006005

Domain	Start	End	E-Value	Type
low complexity region	26	38	N/A	INTRINSIC
low complexity region	50	64	N/A	INTRINSIC
low complexity region	79	101	N/A	INTRINSIC
low complexity region	133	144	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000124973

SMART Domains

Protein: ENSMUSP00000117616
Gene: ENSMUSG00000006005

Domain	Start	End	E-Value	Type
low complexity region	3	14	N/A	INTRINSIC
low complexity region	24	77	N/A	INTRINSIC
coiled coil region	123	444	N/A	INTRINSIC
coiled coil region	497	589	N/A	INTRINSIC
low complexity region	592	608	N/A	INTRINSIC
coiled coil region	613	678	N/A	INTRINSIC
low complexity region	764	777	N/A	INTRINSIC
low complexity region	856	869	N/A	INTRINSIC
low complexity region	885	900	N/A	INTRINSIC
low complexity region	1077	1093	N/A	INTRINSIC
Pfam:TPR_MLP1_2	1112	1240	5.1e-37	PFAM
coiled coil region	1289	1495	N/A	INTRINSIC
low complexity region	1682	1698	N/A	INTRINSIC
internal_repeat_5	1703	1750	8.04e-5	PROSPERO
internal_repeat_3	1704	1765	1.07e-5	PROSPERO
low complexity region	1769	1791	N/A	INTRINSIC
low complexity region	1835	1851	N/A	INTRINSIC
internal_repeat_5	1857	1900	8.04e-5	PROSPERO
internal_repeat_6	1887	1911	8.04e-5	PROSPERO
internal_repeat_3	1893	1955	1.07e-5	PROSPERO
internal_repeat_4	1949	1969	4.1e-5	PROSPERO
internal_repeat_1	1967	1993	1.42e-6	PROSPERO
low complexity region	1994	2007	N/A	INTRINSIC
low complexity region	2016	2055	N/A	INTRINSIC
low complexity region	2063	2088	N/A	INTRINSIC
internal_repeat_4	2091	2110	4.1e-5	PROSPERO
internal_repeat_6	2108	2132	8.04e-5	PROSPERO
low complexity region	2133	2152	N/A	INTRINSIC
internal_repeat_2	2158	2209	2.78e-6	PROSPERO
internal_repeat_1	2228	2253	1.42e-6	PROSPERO
internal_repeat_2	2230	2286	2.78e-6	PROSPERO
low complexity region	2313	2325	N/A	INTRINSIC
low complexity region	2337	2351	N/A	INTRINSIC
low complexity region	2366	2388	N/A	INTRINSIC
low complexity region	2420	2431	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000128417

Predicted Effect

unknown
Transcript: ENSMUST00000161611
AA Change: T874A

SMART Domains

Protein: ENSMUSP00000125677
Gene: ENSMUSG00000006014
AA Change: T874A

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	26	68	9.21e-18	SMART
SO	69	108	2.77e-12	SMART
low complexity region	110	122	N/A	INTRINSIC
low complexity region	127	184	N/A	INTRINSIC
low complexity region	191	212	N/A	INTRINSIC
low complexity region	229	239	N/A	INTRINSIC
internal_repeat_3	244	271	7.42e-5	PROSPERO
low complexity region	277	309	N/A	INTRINSIC
Pfam:Cornifin	578	691	2.2e-9	PFAM
internal_repeat_3	796	825	7.42e-5	PROSPERO
internal_repeat_2	797	823	1.24e-5	PROSPERO
low complexity region	826	879	N/A	INTRINSIC
internal_repeat_2	889	915	1.24e-5	PROSPERO
HX	967	1009	1.67e-7	SMART
HX	1011	1054	3.76e-10	SMART
Blast:HX	1167	1220	6e-27	BLAST

Predicted Effect

unknown
Transcript: ENSMUST00000164600
AA Change: T874A

SMART Domains

Protein: ENSMUSP00000128943
Gene: ENSMUSG00000006014
AA Change: T874A

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	26	68	9.21e-18	SMART
SO	69	108	2.77e-12	SMART
low complexity region	110	122	N/A	INTRINSIC
low complexity region	127	184	N/A	INTRINSIC
low complexity region	191	212	N/A	INTRINSIC
low complexity region	229	239	N/A	INTRINSIC
internal_repeat_3	244	271	7.42e-5	PROSPERO
low complexity region	277	309	N/A	INTRINSIC
Pfam:Cornifin	404	497	2.4e-14	PFAM
Pfam:Cornifin	468	584	3.2e-15	PFAM
Pfam:Cornifin	550	648	3.3e-16	PFAM
Pfam:Cornifin	625	722	2.1e-15	PFAM
Pfam:Cornifin	647	743	7.8e-16	PFAM
Pfam:Cornifin	706	822	1.4e-12	PFAM
low complexity region	826	879	N/A	INTRINSIC
internal_repeat_2	889	915	1.24e-5	PROSPERO
HX	967	1009	1.67e-7	SMART
HX	1011	1054	3.76e-10	SMART
Blast:HX	1167	1220	6e-27	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000159035

SMART Domains

Protein: ENSMUSP00000124410
Gene: ENSMUSG00000006014

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	25	67	2.71e-15	SMART
low complexity region	69	81	N/A	INTRINSIC
low complexity region	88	99	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000161320

SMART Domains

Protein: ENSMUSP00000124801
Gene: ENSMUSG00000006014

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	25	67	3.83e-15	SMART
low complexity region	69	83	N/A	INTRINSIC
low complexity region	100	110	N/A	INTRINSIC
low complexity region	148	179	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000138718

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152967

Predicted Effect

probably benign
Transcript: ENSMUST00000162367

SMART Domains

Protein: ENSMUSP00000125551
Gene: ENSMUSG00000006014

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	26	68	9.21e-18	SMART
SO	69	108	4.21e-12	SMART
low complexity region	110	124	N/A	INTRINSIC
HX	169	211	1.67e-7	SMART
HX	213	256	3.76e-10	SMART
Blast:HX	369	422	2e-27	BLAST

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130779

Meta Mutation Damage Score

0.0966

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.6%

Validation Efficiency

98% (52/53)

MGI Phenotype

PHENOTYPE: Aging homozygous null mice develop a hopping gait, irregular endochondral growth plates, altered cartilage surface, camptodactyly, progressive synovial hyperplasia and, ultimately, joint failure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abr	C	A	11: 76,315,815 (GRCm39)	R583L	probably damaging	Het
Acss1	A	G	2: 150,509,840 (GRCm39)	I93T	probably damaging	Het
Ank3	C	T	10: 69,827,577 (GRCm39)		probably benign	Het
Aoah	A	G	13: 21,184,112 (GRCm39)	Y392C	probably damaging	Het
Atl3	A	T	19: 7,498,163 (GRCm39)	E186D	probably benign	Het
Atp4a	A	G	7: 30,411,887 (GRCm39)	K41E	probably benign	Het
Atp9a	G	A	2: 168,509,933 (GRCm39)	P500L	probably damaging	Het
Ccdc88b	A	T	19: 6,832,246 (GRCm39)	V363D	possibly damaging	Het
Cftr	T	C	6: 18,258,235 (GRCm39)	V532A	probably damaging	Het
Cldn13	T	C	5: 134,943,769 (GRCm39)	T139A	possibly damaging	Het
Cnksr3	T	C	10: 7,076,820 (GRCm39)	Y124C	probably benign	Het
Cyb5r2	T	C	7: 107,352,462 (GRCm39)	K161E	possibly damaging	Het
Epop	T	C	11: 97,519,333 (GRCm39)	S259G	possibly damaging	Het
Fhl2	G	A	1: 43,162,813 (GRCm39)	T234I	possibly damaging	Het
Fnip2	T	C	3: 79,388,941 (GRCm39)	T567A	possibly damaging	Het
Frmd4b	A	G	6: 97,464,601 (GRCm39)	C39R	probably damaging	Het
Grhl3	T	C	4: 135,284,744 (GRCm39)	N116S	possibly damaging	Het
Igkv14-111	A	T	6: 68,233,725 (GRCm39)	R75S	probably benign	Het
Il16	A	T	7: 83,371,529 (GRCm39)	D92E	probably damaging	Het
Il16	C	A	7: 83,371,536 (GRCm39)	G90V	probably damaging	Het
Ipo11	T	C	13: 107,002,277 (GRCm39)		probably null	Het
Kng2	A	G	16: 22,830,865 (GRCm39)	I148T	probably damaging	Het
Lrrc41	T	C	4: 115,945,977 (GRCm39)	S231P	possibly damaging	Het
Maneal	C	T	4: 124,750,635 (GRCm39)	V374I	possibly damaging	Het
Mgat4c	T	A	10: 102,220,988 (GRCm39)	L90Q	probably damaging	Het
Mrps28	A	G	3: 8,965,040 (GRCm39)		probably null	Het
Ncapd3	A	G	9: 26,963,051 (GRCm39)	D452G	probably benign	Het
Nefh	T	C	11: 4,889,551 (GRCm39)	T1023A	unknown	Het
Nipa1	A	G	7: 55,629,354 (GRCm39)	V253A	probably benign	Het
Or5ac17	C	T	16: 59,036,383 (GRCm39)	V198M	probably benign	Het
Or5b97	G	T	19: 12,878,369 (GRCm39)	F258L	possibly damaging	Het
Or5m11	G	T	2: 85,781,862 (GRCm39)	G152C	probably damaging	Het
Or8d23	A	G	9: 38,841,961 (GRCm39)	S165G	probably benign	Het
Or8k41	T	A	2: 86,313,573 (GRCm39)	H171L	probably benign	Het
Pak1	A	G	7: 97,557,088 (GRCm39)	D495G	probably benign	Het
Pcm1	G	A	8: 41,714,073 (GRCm39)	R213H	probably damaging	Het
Proser1	C	T	3: 53,385,750 (GRCm39)	T544M	possibly damaging	Het
Rftn1	T	C	17: 50,354,334 (GRCm39)	M343V	probably benign	Het
Ryr1	T	C	7: 28,715,079 (GRCm39)	I4656V	probably benign	Het
Scg2	T	A	1: 79,414,007 (GRCm39)	N239Y	probably damaging	Het
Sec16a	C	A	2: 26,313,512 (GRCm39)	M1949I	probably benign	Het
Sipa1l2	A	G	8: 126,171,223 (GRCm39)		probably null	Het
Slc10a2	A	C	8: 5,148,581 (GRCm39)		probably null	Het
Slc25a16	C	T	10: 62,773,256 (GRCm39)	Q164*	probably null	Het
Specc1l	T	C	10: 75,082,001 (GRCm39)	Y483H	probably damaging	Het
Ston1	T	C	17: 88,943,896 (GRCm39)	V434A	probably benign	Het
Tarbp2	A	G	15: 102,426,914 (GRCm39)		probably benign	Het
Trappc8	C	T	18: 20,969,925 (GRCm39)	V1022M	probably benign	Het
Tsen54	T	C	11: 115,712,506 (GRCm39)	V269A	probably damaging	Het
Vps13a	A	T	19: 16,641,382 (GRCm39)	M78K	possibly damaging	Het
Vwa5b2	A	G	16: 20,413,429 (GRCm39)	T215A	probably damaging	Het
Zc3h7a	A	G	16: 10,971,025 (GRCm39)	Y335H	probably damaging	Het
Zfp994	T	A	17: 22,419,527 (GRCm39)	Q474L	possibly damaging	Het

Other mutations in Prg4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00493:Prg4	APN	1	150,327,671 (GRCm39)	missense	probably damaging	0.99
IGL02033:Prg4	APN	1	150,331,619 (GRCm39)	intron	probably benign
IGL02154:Prg4	APN	1	150,330,613 (GRCm39)	intron	probably benign
IGL03111:Prg4	APN	1	150,327,653 (GRCm39)	missense	probably benign	0.06
IGL03177:Prg4	APN	1	150,331,354 (GRCm39)	intron	probably benign
IGL03260:Prg4	APN	1	150,331,378 (GRCm39)	intron	probably benign
IGL03281:Prg4	APN	1	150,325,839 (GRCm39)	splice site	probably benign
R0046:Prg4	UTSW	1	150,331,837 (GRCm39)	missense	possibly damaging	0.53
R0046:Prg4	UTSW	1	150,331,837 (GRCm39)	missense	possibly damaging	0.53
R0196:Prg4	UTSW	1	150,330,243 (GRCm39)	intron	probably benign
R0233:Prg4	UTSW	1	150,329,298 (GRCm39)	splice site	probably benign
R0255:Prg4	UTSW	1	150,331,558 (GRCm39)	intron	probably benign
R0616:Prg4	UTSW	1	150,336,462 (GRCm39)	missense	probably damaging	1.00
R1016:Prg4	UTSW	1	150,330,442 (GRCm39)	intron	probably benign
R1826:Prg4	UTSW	1	150,327,760 (GRCm39)	missense	probably benign	0.09
R1862:Prg4	UTSW	1	150,336,420 (GRCm39)	missense	probably damaging	0.99
R1863:Prg4	UTSW	1	150,336,420 (GRCm39)	missense	probably damaging	0.99
R1922:Prg4	UTSW	1	150,325,750 (GRCm39)	nonsense	probably null
R1940:Prg4	UTSW	1	150,331,774 (GRCm39)	missense	possibly damaging	0.53
R3765:Prg4	UTSW	1	150,327,122 (GRCm39)	missense	probably damaging	0.97
R3855:Prg4	UTSW	1	150,327,751 (GRCm39)	missense	probably damaging	1.00
R3894:Prg4	UTSW	1	150,330,510 (GRCm39)	intron	probably benign
R3895:Prg4	UTSW	1	150,330,510 (GRCm39)	intron	probably benign
R3912:Prg4	UTSW	1	150,327,619 (GRCm39)	missense	probably damaging	1.00
R3935:Prg4	UTSW	1	150,333,908 (GRCm39)	missense	possibly damaging	0.68
R4050:Prg4	UTSW	1	150,330,510 (GRCm39)	intron	probably benign
R4475:Prg4	UTSW	1	150,330,610 (GRCm39)	intron	probably benign
R4794:Prg4	UTSW	1	150,330,297 (GRCm39)	intron	probably benign
R4910:Prg4	UTSW	1	150,331,574 (GRCm39)	intron	probably benign
R4911:Prg4	UTSW	1	150,331,574 (GRCm39)	intron	probably benign
R4993:Prg4	UTSW	1	150,336,432 (GRCm39)	missense	probably damaging	1.00
R5378:Prg4	UTSW	1	150,330,977 (GRCm39)	intron	probably benign
R5381:Prg4	UTSW	1	150,330,204 (GRCm39)	intron	probably benign
R5452:Prg4	UTSW	1	150,331,519 (GRCm39)	intron	probably benign
R5870:Prg4	UTSW	1	150,331,300 (GRCm39)	nonsense	probably null
R5888:Prg4	UTSW	1	150,328,101 (GRCm39)	missense	probably damaging	1.00
R5929:Prg4	UTSW	1	150,329,880 (GRCm39)	missense	probably benign	0.01
R6058:Prg4	UTSW	1	150,327,197 (GRCm39)	missense	probably damaging	0.99
R6059:Prg4	UTSW	1	150,325,748 (GRCm39)	missense	possibly damaging	0.67
R6232:Prg4	UTSW	1	150,331,567 (GRCm39)	intron	probably benign
R6272:Prg4	UTSW	1	150,330,517 (GRCm39)	intron	probably benign
R6659:Prg4	UTSW	1	150,336,432 (GRCm39)	missense	probably damaging	1.00
R6663:Prg4	UTSW	1	150,330,852 (GRCm39)	intron	probably benign
R6882:Prg4	UTSW	1	150,329,246 (GRCm39)	missense	probably damaging	1.00
R6970:Prg4	UTSW	1	150,331,657 (GRCm39)	intron	probably benign
R7078:Prg4	UTSW	1	150,334,014 (GRCm39)	missense	possibly damaging	0.91
R7102:Prg4	UTSW	1	150,328,005 (GRCm39)	missense	probably damaging	1.00
R7264:Prg4	UTSW	1	150,329,818 (GRCm39)	missense	not run
R7487:Prg4	UTSW	1	150,331,656 (GRCm39)	missense	unknown
R7531:Prg4	UTSW	1	150,330,786 (GRCm39)	missense	unknown
R7651:Prg4	UTSW	1	150,330,696 (GRCm39)	missense	unknown
R7701:Prg4	UTSW	1	150,333,293 (GRCm39)	missense	possibly damaging	0.53
R8072:Prg4	UTSW	1	150,331,774 (GRCm39)	missense	possibly damaging	0.53
R8168:Prg4	UTSW	1	150,331,601 (GRCm39)	missense	unknown
R8248:Prg4	UTSW	1	150,330,877 (GRCm39)	missense	unknown
R8436:Prg4	UTSW	1	150,331,318 (GRCm39)	missense	unknown
R8460:Prg4	UTSW	1	150,331,692 (GRCm39)	missense	possibly damaging	0.83
R8514:Prg4	UTSW	1	150,330,396 (GRCm39)	missense	unknown
R8904:Prg4	UTSW	1	150,331,810 (GRCm39)	missense	possibly damaging	0.83
R9072:Prg4	UTSW	1	150,331,288 (GRCm39)	missense	unknown
R9073:Prg4	UTSW	1	150,331,288 (GRCm39)	missense	unknown
R9274:Prg4	UTSW	1	150,331,924 (GRCm39)	missense	possibly damaging	0.53
R9337:Prg4	UTSW	1	150,327,116 (GRCm39)	missense	probably damaging	1.00
R9488:Prg4	UTSW	1	150,327,024 (GRCm39)	missense	probably benign
R9613:Prg4	UTSW	1	150,331,660 (GRCm39)	missense	unknown
R9670:Prg4	UTSW	1	150,326,618 (GRCm39)	missense	probably benign	0.01
X0024:Prg4	UTSW	1	150,330,243 (GRCm39)	intron	probably benign

Predicted Primers

PCR Primer
(F):5'- GGCCAAAAGATCAGTGCCTG -3'
(R):5'- TCCAGCAGAAGAGATTCAGAAC -3'

Sequencing Primer
(F):5'- ATCAGTGCCTGGAATAGCTG -3'
(R):5'- TGAAGAAACAACTCCTGCATCAG -3'

Posted On

2018-05-21