Mutagenetix > Incidental Mutation

Incidental Mutation 'R6464:Or10ag55-ps1'

517715

Institutional Source

Beutler Lab

Gene Symbol

Or10ag55-ps1

Ensembl Gene

ENSMUSG00000083706

Gene Name

olfactory receptor family 10 subfamily AG member 55, pseudogene 1

Synonyms

GA_x6K02T2Q125-48769330-48770299, Olfr1117-ps1, MOR264-27_p, MOR264-10P

MMRRC Submission

044597-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R6464 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

87114651-87115618 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 87139951 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 273 (I273F)

Ref Sequence

ENSEMBL: ENSMUSP00000144143 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000121296] [ENSMUST00000216396]

AlphaFold

A0A1L1STN9

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000120328

Predicted Effect

probably damaging
Transcript: ENSMUST00000121296
AA Change: I273F

PolyPhen 2 Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000144143
Gene: ENSMUSG00000083706
AA Change: I273F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	22	298	1.2e-51	PFAM
Pfam:7tm_1	32	281	7.2e-21	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000216396
AA Change: I293F

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

Meta Mutation Damage Score

0.3552

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.8%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc1	T	A	16: 14,265,354 (GRCm39)	S769T	probably damaging	Het
Apoe	G	A	7: 19,431,461 (GRCm39)	T52M	probably damaging	Het
Arhgef10l	A	C	4: 140,314,126 (GRCm39)	M230R	probably benign	Het
Cdkl4	A	T	17: 80,832,781 (GRCm39)	I321N	probably benign	Het
Ceacam5	T	C	7: 17,481,391 (GRCm39)		probably null	Het
Cfap70	T	C	14: 20,451,107 (GRCm39)		probably null	Het
Clasp2	A	G	9: 113,602,785 (GRCm39)	Y195C	probably damaging	Het
Clip2	T	C	5: 134,520,779 (GRCm39)	I999V	probably benign	Het
Cntnap5a	A	G	1: 116,112,138 (GRCm39)	D476G	probably benign	Het
Col6a6	C	T	9: 105,666,152 (GRCm39)	M1I	probably null	Het
Cyp27a1	T	C	1: 74,775,047 (GRCm39)	V292A	possibly damaging	Het
Cyp2c29	A	G	19: 39,317,669 (GRCm39)	Y385C	probably damaging	Het
Dsg3	A	C	18: 20,666,583 (GRCm39)	R597S	probably benign	Het
Fam131c	T	C	4: 141,109,653 (GRCm39)	I95T	probably damaging	Het
Fam13b	A	T	18: 34,606,684 (GRCm39)	C302*	probably null	Het
Fkbp15	T	C	4: 62,226,315 (GRCm39)	K745E	possibly damaging	Het
Flg	T	A	3: 93,188,688 (GRCm39)		probably benign	Het
Gm11559	T	C	11: 99,755,542 (GRCm39)	C64R	unknown	Het
Helt	T	C	8: 46,745,571 (GRCm39)	D104G	probably damaging	Het
Helz2	T	C	2: 180,876,862 (GRCm39)	T1211A	probably benign	Het
Ifna9	T	C	4: 88,510,487 (GRCm39)	R46G	possibly damaging	Het
Kif20b	G	A	19: 34,911,841 (GRCm39)	V235I	probably benign	Het
Kif7	A	G	7: 79,363,842 (GRCm39)	V22A	possibly damaging	Het
Klra9	G	T	6: 130,155,995 (GRCm39)	Y253*	probably null	Het
Lipf	A	T	19: 33,950,944 (GRCm39)	Y305F	probably benign	Het
Lpar1	A	G	4: 58,486,875 (GRCm39)	L132P	possibly damaging	Het
Magi1	A	T	6: 93,676,770 (GRCm39)	V834E	probably damaging	Het
Meltf	T	C	16: 31,709,594 (GRCm39)	Y432H	probably benign	Het
Mroh2a	G	C	1: 88,185,524 (GRCm39)	E1510D	probably damaging	Het
Myo1f	T	C	17: 33,795,621 (GRCm39)	L59P	probably damaging	Het
Naa25	G	A	5: 121,556,024 (GRCm39)	D271N	probably damaging	Het
Or8u3-ps	A	T	2: 85,952,752 (GRCm39)	I162F	possibly damaging	Het
Otoa	A	G	7: 120,701,828 (GRCm39)	Q169R	probably damaging	Het
Pde4dip	C	T	3: 97,617,660 (GRCm39)	D1723N	probably damaging	Het
Ptdss2	T	C	7: 140,732,124 (GRCm39)	V175A	probably damaging	Het
Rdh8	G	T	9: 20,734,696 (GRCm39)	R121L	probably damaging	Het
Rpl23	T	C	11: 97,669,111 (GRCm39)		probably null	Het
Scn5a	T	C	9: 119,363,646 (GRCm39)	D498G	probably damaging	Het
Slc39a14	G	C	14: 70,544,177 (GRCm39)	L470V	probably damaging	Het
Smok2a	C	T	17: 13,445,317 (GRCm39)	A298V	probably damaging	Het
Tesk2	A	C	4: 116,660,046 (GRCm39)	D388A	probably damaging	Het
Tnik	G	A	3: 28,666,119 (GRCm39)		probably null	Het
Ube3a	T	A	7: 58,925,931 (GRCm39)	Y236*	probably null	Het
Ugt1a1	CAGAGAGAGAGAGA	CAGAGAGAGAGA	1: 88,139,706 (GRCm39)		probably benign	Het
Vinac1	T	C	2: 128,881,465 (GRCm39)	T154A	possibly damaging	Het
Vmn1r74	A	T	7: 11,581,131 (GRCm39)	I144L	possibly damaging	Het
Vmn2r1	G	A	3: 64,008,766 (GRCm39)	D482N	probably benign	Het
Vwf	T	C	6: 125,616,363 (GRCm39)		probably null	Het
Wdr97	A	G	15: 76,246,977 (GRCm39)	T1413A	probably benign	Het
Zfp703	C	G	8: 27,469,355 (GRCm39)	P340A	probably damaging	Het
Znfx1	T	C	2: 166,888,842 (GRCm39)	S789G	probably benign	Het

Other mutations in Or10ag55-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R6154:Or10ag55-ps1	UTSW	2	87,139,902 (GRCm39)	missense	probably benign	0.01
R6494:Or10ag55-ps1	UTSW	2	87,139,520 (GRCm39)	missense	possibly damaging	0.59
R6692:Or10ag55-ps1	UTSW	2	87,139,336 (GRCm39)	missense	possibly damaging	0.95
R6826:Or10ag55-ps1	UTSW	2	87,114,672 (GRCm39)	missense	probably benign	0.02
R7054:Or10ag55-ps1	UTSW	2	87,114,775 (GRCm39)	missense	probably benign	0.42
R7176:Or10ag55-ps1	UTSW	2	87,115,378 (GRCm39)	missense	probably damaging	0.99
R7271:Or10ag55-ps1	UTSW	2	87,114,725 (GRCm39)	missense	probably benign	0.23
R7616:Or10ag55-ps1	UTSW	2	87,115,617 (GRCm39)	makesense	probably null
R8434:Or10ag55-ps1	UTSW	2	87,114,978 (GRCm39)	missense	probably benign	0.01
R8673:Or10ag55-ps1	UTSW	2	87,114,658 (GRCm39)	missense	probably benign	0.05
R8733:Or10ag55-ps1	UTSW	2	87,115,116 (GRCm39)	missense	probably benign	0.24
R8981:Or10ag55-ps1	UTSW	2	87,115,561 (GRCm39)	missense	probably benign	0.01
R9567:Or10ag55-ps1	UTSW	2	87,115,037 (GRCm39)	nonsense	probably null
R9654:Or10ag55-ps1	UTSW	2	87,115,071 (GRCm39)	missense	probably benign	0.12
R9716:Or10ag55-ps1	UTSW	2	87,115,491 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGAAACTGTCTTCAGCCAAAG -3'
(R):5'- ACACTGAGTTGAACAAGTAACCTAG -3'

Sequencing Primer
(F):5'- AAAGGGATGGCCAAGGCTTTTTC -3'
(R):5'- TGTGAGCATAGATGGATATGA -3'

Posted On

2018-05-21