Mutagenetix > Incidental Mutation

Incidental Mutation 'R6418:Vmn2r68'

518073

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r68

Ensembl Gene

ENSMUSG00000096861

Gene Name

vomeronasal 2, receptor 68

Synonyms

EG620697, Vmn2r68-ps

MMRRC Submission

044560-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R6418 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

84870726-84886912 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 84882915 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 279 (H279L)

Ref Sequence

ENSEMBL: ENSMUSP00000129411 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061074]

AlphaFold

L7N2B3

Predicted Effect

probably benign
Transcript: ENSMUST00000061074
AA Change: H279L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000129411
Gene: ENSMUSG00000096861
AA Change: H279L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	463	4.5e-28	PFAM
Pfam:NCD3G	507	559	1.1e-18	PFAM
Pfam:7tm_3	589	827	3.7e-53	PFAM

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 93.8%

Validation Efficiency

99% (67/68)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930507D05Rik	C	T	10: 62,285,441 (GRCm39)	A55V	unknown	Het
Adra1b	T	G	11: 43,667,028 (GRCm39)	D403A	probably benign	Het
Ankdd1b	A	T	13: 96,597,405 (GRCm39)	L9Q	probably damaging	Het
Ankrd17	A	T	5: 90,426,204 (GRCm39)	S789R	possibly damaging	Het
B3galnt1	A	G	3: 69,482,326 (GRCm39)	S312P	probably damaging	Het
C4bp	C	T	1: 130,583,750 (GRCm39)	V73M	probably damaging	Het
Ccdc121rt2	T	C	5: 112,598,019 (GRCm39)	S189P	probably benign	Het
Cenpe	T	A	3: 134,957,305 (GRCm39)	N1854K	probably damaging	Het
Chrm2	A	C	6: 36,500,674 (GRCm39)	Y177S	probably damaging	Het
Clca4b	C	T	3: 144,633,996 (GRCm39)	V140I	probably benign	Het
Csad	A	G	15: 102,087,958 (GRCm39)	S322P	probably damaging	Het
Depdc1b	T	G	13: 108,493,956 (GRCm39)	Y115D	probably damaging	Het
Dnah17	T	C	11: 118,020,023 (GRCm39)	Y8C	probably damaging	Het
Dsg3	T	C	18: 20,656,817 (GRCm39)		probably null	Het
Elf5	A	G	2: 103,269,697 (GRCm39)	T93A	possibly damaging	Het
Eogt	C	T	6: 97,122,353 (GRCm39)	D27N	possibly damaging	Het
Fam118b	T	C	9: 35,146,633 (GRCm39)	D78G	probably damaging	Het
Fhip2a	T	A	19: 57,370,166 (GRCm39)	M470K	probably benign	Het
Frem3	A	G	8: 81,337,781 (GRCm39)	T25A	probably benign	Het
Gab1	C	A	8: 81,515,226 (GRCm39)	R364L	possibly damaging	Het
Golm1	T	C	13: 59,813,375 (GRCm39)	Y32C	probably damaging	Het
Has1	A	T	17: 18,070,207 (GRCm39)	V238E	probably damaging	Het
Hkdc1	T	C	10: 62,219,583 (GRCm39)	R907G	possibly damaging	Het
Igkv4-61	C	A	6: 69,394,138 (GRCm39)	A31S	possibly damaging	Het
Krtap24-1	C	T	16: 88,408,761 (GRCm39)	V122I	probably damaging	Het
Lrrc10	T	G	10: 116,881,616 (GRCm39)	L97V	probably damaging	Het
Map3k20	T	A	2: 72,232,457 (GRCm39)	M371K	probably benign	Het
Mis18bp1	A	T	12: 65,205,317 (GRCm39)	M2K	possibly damaging	Het
Mob3b	T	A	4: 34,954,049 (GRCm39)		probably null	Het
Muc4	A	G	16: 32,570,607 (GRCm39)	T556A	possibly damaging	Het
Muc6	A	C	7: 141,224,032 (GRCm39)		probably benign	Het
Ncapg	A	T	5: 45,839,158 (GRCm39)	L519F	probably damaging	Het
Nfix	A	G	8: 85,453,778 (GRCm39)	S265P	probably benign	Het
Or13p10	T	A	4: 118,522,808 (GRCm39)	F31L	possibly damaging	Het
Or4c121	T	C	2: 89,023,823 (GRCm39)	K185R	probably damaging	Het
Or4k49	T	C	2: 111,494,817 (GRCm39)	V82A	probably benign	Het
Or52e5	T	C	7: 104,719,514 (GRCm39)	L280P	probably damaging	Het
Or8g35	C	A	9: 39,381,112 (GRCm39)	K303N	probably benign	Het
Osgin2	T	C	4: 16,006,410 (GRCm39)	T95A	probably benign	Het
Parp4	T	C	14: 56,858,108 (GRCm39)		probably null	Het
Pcdh7	G	A	5: 57,879,046 (GRCm39)	G867D	probably damaging	Het
Pcdhb22	T	C	18: 37,652,959 (GRCm39)	S476P	possibly damaging	Het
Phldb1	A	G	9: 44,623,197 (GRCm39)	I619T	probably damaging	Het
Ppp1r13l	C	T	7: 19,105,256 (GRCm39)	R343C	probably damaging	Het
Rab2b	T	A	14: 52,506,164 (GRCm39)		probably benign	Het
Rims2	A	T	15: 39,373,092 (GRCm39)	E972V	probably damaging	Het
Slc26a9	G	T	1: 131,686,228 (GRCm39)	C337F	probably benign	Het
Spty2d1	A	G	7: 46,647,751 (GRCm39)	S393P	probably damaging	Het
Stkld1	A	T	2: 26,831,093 (GRCm39)	N90I	possibly damaging	Het
Svep1	T	A	4: 58,053,126 (GRCm39)	R3406S	probably benign	Het
Svil	A	T	18: 5,040,171 (GRCm39)	D48V	probably benign	Het
Thsd7a	T	A	6: 12,555,081 (GRCm39)	K268*	probably null	Het
Tmc6	A	G	11: 117,661,326 (GRCm39)	L618P	probably damaging	Het
Tmem231	G	A	8: 112,653,524 (GRCm39)		probably benign	Het
Ube2q2l	C	A	6: 136,378,099 (GRCm39)	D244Y	probably damaging	Het
Ubn1	A	G	16: 4,899,791 (GRCm39)	H1101R	probably benign	Het
Upf2	G	A	2: 6,032,150 (GRCm39)	V899I	unknown	Het
Ush2a	A	T	1: 188,360,763 (GRCm39)	N2161I	probably damaging	Het
Usp25	A	T	16: 76,859,330 (GRCm39)	D276V	probably damaging	Het
Vps13d	G	A	4: 144,818,850 (GRCm39)	R3019W	probably damaging	Het
Vsig10	A	T	5: 117,486,361 (GRCm39)	E423D	probably benign	Het
Wdfy2	T	A	14: 63,162,582 (GRCm39)	F95I	probably benign	Het
Xrn1	A	G	9: 95,915,763 (GRCm39)		probably null	Het
Zdhhc13	A	G	7: 48,461,149 (GRCm39)	T362A	possibly damaging	Het
Zfp984	C	T	4: 147,845,703 (GRCm39)	S2N	probably benign	Het

Other mutations in Vmn2r68

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01391:Vmn2r68	APN	7	84,886,819 (GRCm39)	missense	probably benign
IGL01477:Vmn2r68	APN	7	84,882,691 (GRCm39)	missense	probably damaging	1.00
IGL01600:Vmn2r68	APN	7	84,871,468 (GRCm39)	missense	probably benign	0.39
IGL01979:Vmn2r68	APN	7	84,871,325 (GRCm39)	missense	probably benign
IGL01999:Vmn2r68	APN	7	84,871,439 (GRCm39)	missense	probably damaging	1.00
IGL02269:Vmn2r68	APN	7	84,870,947 (GRCm39)	missense	possibly damaging	0.84
IGL02517:Vmn2r68	APN	7	84,871,153 (GRCm39)	nonsense	probably null
IGL02827:Vmn2r68	APN	7	84,886,800 (GRCm39)	missense	probably damaging	1.00
IGL02852:Vmn2r68	APN	7	84,882,595 (GRCm39)	missense	probably damaging	1.00
IGL02982:Vmn2r68	APN	7	84,883,649 (GRCm39)	missense	probably benign	0.12
IGL03099:Vmn2r68	APN	7	84,871,448 (GRCm39)	nonsense	probably null
IGL03166:Vmn2r68	APN	7	84,871,331 (GRCm39)	missense	probably benign	0.01
IGL03168:Vmn2r68	APN	7	84,870,972 (GRCm39)	missense	probably damaging	1.00
IGL03243:Vmn2r68	APN	7	84,882,963 (GRCm39)	missense	possibly damaging	0.66
F5770:Vmn2r68	UTSW	7	84,871,088 (GRCm39)	missense	probably benign	0.01
R0280:Vmn2r68	UTSW	7	84,882,466 (GRCm39)	critical splice donor site	probably null
R0280:Vmn2r68	UTSW	7	84,882,457 (GRCm39)	splice site	probably benign
R0281:Vmn2r68	UTSW	7	84,882,466 (GRCm39)	critical splice donor site	probably null
R0281:Vmn2r68	UTSW	7	84,882,457 (GRCm39)	splice site	probably benign
R0348:Vmn2r68	UTSW	7	84,870,884 (GRCm39)	missense	possibly damaging	0.50
R0390:Vmn2r68	UTSW	7	84,882,466 (GRCm39)	critical splice donor site	probably null
R0390:Vmn2r68	UTSW	7	84,882,457 (GRCm39)	splice site	probably benign
R0722:Vmn2r68	UTSW	7	84,870,794 (GRCm39)	missense	possibly damaging	0.95
R1129:Vmn2r68	UTSW	7	84,886,712 (GRCm39)	splice site	probably null
R1136:Vmn2r68	UTSW	7	84,871,549 (GRCm39)	missense	possibly damaging	0.81
R1319:Vmn2r68	UTSW	7	84,881,700 (GRCm39)	missense	probably damaging	0.96
R1614:Vmn2r68	UTSW	7	84,870,946 (GRCm39)	missense	possibly damaging	0.93
R1682:Vmn2r68	UTSW	7	84,882,574 (GRCm39)	missense	possibly damaging	0.68
R1837:Vmn2r68	UTSW	7	84,882,886 (GRCm39)	missense	probably damaging	0.96
R1893:Vmn2r68	UTSW	7	84,883,867 (GRCm39)	nonsense	probably null
R1908:Vmn2r68	UTSW	7	84,883,260 (GRCm39)	missense	probably benign	0.09
R1909:Vmn2r68	UTSW	7	84,883,260 (GRCm39)	missense	probably benign	0.09
R1951:Vmn2r68	UTSW	7	84,883,102 (GRCm39)	missense	probably damaging	1.00
R2177:Vmn2r68	UTSW	7	84,871,123 (GRCm39)	missense	probably benign	0.01
R2178:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R2185:Vmn2r68	UTSW	7	84,882,901 (GRCm39)	nonsense	probably null
R2188:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R2282:Vmn2r68	UTSW	7	84,870,859 (GRCm39)	missense	possibly damaging	0.65
R2567:Vmn2r68	UTSW	7	84,883,803 (GRCm39)	missense	probably benign
R2869:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2869:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2870:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2870:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2871:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2871:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2873:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R2874:Vmn2r68	UTSW	7	84,882,834 (GRCm39)	missense	probably benign	0.25
R3149:Vmn2r68	UTSW	7	84,886,875 (GRCm39)	missense	probably benign	0.00
R3401:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R3978:Vmn2r68	UTSW	7	84,881,670 (GRCm39)	missense	probably benign	0.00
R4399:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4401:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4421:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4478:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4479:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4495:Vmn2r68	UTSW	7	84,870,758 (GRCm39)	frame shift	probably null
R4628:Vmn2r68	UTSW	7	84,883,673 (GRCm39)	missense	probably benign	0.00
R4649:Vmn2r68	UTSW	7	84,870,743 (GRCm39)	missense	probably benign
R4654:Vmn2r68	UTSW	7	84,882,769 (GRCm39)	nonsense	probably null
R4793:Vmn2r68	UTSW	7	84,883,648 (GRCm39)	missense	probably benign	0.01
R5007:Vmn2r68	UTSW	7	84,881,622 (GRCm39)	missense	probably benign
R5021:Vmn2r68	UTSW	7	84,882,942 (GRCm39)	missense	possibly damaging	0.62
R5082:Vmn2r68	UTSW	7	84,883,076 (GRCm39)	missense	probably benign	0.12
R5177:Vmn2r68	UTSW	7	84,871,199 (GRCm39)	missense	probably damaging	0.99
R5221:Vmn2r68	UTSW	7	84,871,085 (GRCm39)	missense	probably damaging	1.00
R5514:Vmn2r68	UTSW	7	84,886,767 (GRCm39)	missense	possibly damaging	0.92
R5521:Vmn2r68	UTSW	7	84,882,926 (GRCm39)	missense	probably benign	0.03
R5563:Vmn2r68	UTSW	7	84,871,283 (GRCm39)	missense	probably damaging	1.00
R5664:Vmn2r68	UTSW	7	84,882,978 (GRCm39)	missense	probably benign	0.02
R5829:Vmn2r68	UTSW	7	84,886,812 (GRCm39)	missense	probably benign	0.00
R6016:Vmn2r68	UTSW	7	84,871,453 (GRCm39)	missense	probably damaging	0.99
R6356:Vmn2r68	UTSW	7	84,883,048 (GRCm39)	missense	possibly damaging	0.85
R6413:Vmn2r68	UTSW	7	84,870,973 (GRCm39)	missense	probably damaging	1.00
R6699:Vmn2r68	UTSW	7	84,881,583 (GRCm39)	missense	possibly damaging	0.58
R7287:Vmn2r68	UTSW	7	84,871,460 (GRCm39)	missense	probably benign	0.33
R7319:Vmn2r68	UTSW	7	84,883,042 (GRCm39)	missense	probably benign
R7374:Vmn2r68	UTSW	7	84,881,607 (GRCm39)	missense	possibly damaging	0.66
R7585:Vmn2r68	UTSW	7	84,881,587 (GRCm39)	missense	probably damaging	1.00
R7605:Vmn2r68	UTSW	7	84,883,116 (GRCm39)	missense	probably benign	0.01
R7892:Vmn2r68	UTSW	7	84,883,722 (GRCm39)	missense	probably benign
R7979:Vmn2r68	UTSW	7	84,883,625 (GRCm39)	critical splice donor site	probably null
R8177:Vmn2r68	UTSW	7	84,871,422 (GRCm39)	nonsense	probably null
R8349:Vmn2r68	UTSW	7	84,882,785 (GRCm39)	missense	probably damaging	1.00
R8378:Vmn2r68	UTSW	7	84,871,108 (GRCm39)	missense	probably benign	0.00
R8397:Vmn2r68	UTSW	7	84,886,722 (GRCm39)	missense	possibly damaging	0.71
R8449:Vmn2r68	UTSW	7	84,882,785 (GRCm39)	missense	probably damaging	1.00
R8543:Vmn2r68	UTSW	7	84,883,648 (GRCm39)	missense	probably benign	0.01
R8680:Vmn2r68	UTSW	7	84,871,321 (GRCm39)	missense	possibly damaging	0.68
R9056:Vmn2r68	UTSW	7	84,871,420 (GRCm39)	missense	possibly damaging	0.71
R9342:Vmn2r68	UTSW	7	84,882,993 (GRCm39)	missense	probably benign	0.39
R9734:Vmn2r68	UTSW	7	84,882,757 (GRCm39)	missense	possibly damaging	0.54
V7581:Vmn2r68	UTSW	7	84,871,088 (GRCm39)	missense	probably benign	0.01
Z1176:Vmn2r68	UTSW	7	84,871,289 (GRCm39)	missense	probably benign	0.27
Z1176:Vmn2r68	UTSW	7	84,870,941 (GRCm39)	missense	probably damaging	1.00
Z1176:Vmn2r68	UTSW	7	84,871,307 (GRCm39)	missense	possibly damaging	0.92

Predicted Primers

PCR Primer
(F):5'- CACTACTGTAATTTGAAGGGTGTACTG -3'
(R):5'- AGCGATGGTTTCCCTAATGG -3'

Sequencing Primer
(F):5'- AAGGGTGTACTGTTTGGATAAATTG -3'
(R):5'- CCCTAATGGTTCATTTCAGATGG -3'

Posted On

2018-05-24