Mutagenetix > Incidental Mutation

Incidental Mutation 'R6444:Mrgprx1'

519128

Institutional Source

Beutler Lab

Gene Symbol

Mrgprx1

Ensembl Gene

ENSMUSG00000070552

Gene Name

MAS-related GPR, member X1

Synonyms

Mrgprc11, MrgC11

MMRRC Submission

044582-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6444 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

47670719-47677345 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 47671562 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 62 (V62I)

Ref Sequence

ENSEMBL: ENSMUSP00000091954 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094390]

AlphaFold

Q8CIP3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094390
AA Change: V62I

PolyPhen 2 Score 0.872 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000091954
Gene: ENSMUSG00000070552
AA Change: V62I

Domain	Start	End	E-Value	Type
Pfam:7tm_1	43	202	1.9e-7	PFAM
low complexity region	227	245	N/A	INTRINSIC

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.4%

Validation Efficiency

100% (37/37)

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A430033K04Rik	ACAGAGCAGTGCCTACCAG	ACAG	5: 138,637,831 (GRCm39)		probably benign	Het
Bcas2	T	A	3: 103,079,362 (GRCm39)		probably null	Het
Camk1d	A	T	2: 5,317,956 (GRCm39)	I233N	probably damaging	Het
Chd2	T	C	7: 73,150,785 (GRCm39)		probably null	Het
Cnot2	T	C	10: 116,335,260 (GRCm39)	D246G	probably benign	Het
Cped1	A	C	6: 21,986,930 (GRCm39)	I41L	probably benign	Het
Dcdc2c	A	G	12: 28,585,475 (GRCm39)	V174A	probably damaging	Het
Dscam	G	A	16: 96,420,844 (GRCm39)	R1681C	probably damaging	Het
Dysf	G	A	6: 84,167,822 (GRCm39)	V1755M	probably benign	Het
Eif5b	G	A	1: 38,075,292 (GRCm39)	D590N	probably damaging	Het
Fntb	A	G	12: 76,963,214 (GRCm39)	Y399C	probably damaging	Het
Galns	T	C	8: 123,338,077 (GRCm39)	M1V	probably null	Het
Galnt15	T	C	14: 31,762,368 (GRCm39)	F199L	probably damaging	Het
Gm8229	A	T	14: 44,602,928 (GRCm39)	H38L	unknown	Het
Magel2	T	C	7: 62,029,747 (GRCm39)	Y884H	unknown	Het
Miga1	A	T	3: 151,989,468 (GRCm39)	V473E	probably damaging	Het
Mybl1	G	A	1: 9,755,917 (GRCm39)	P211S	possibly damaging	Het
Myo19	A	G	11: 84,786,134 (GRCm39)	H254R	probably benign	Het
Myo1h	A	G	5: 114,453,017 (GRCm39)	T6A	possibly damaging	Het
Ncbp2	CGTCTGGATG	CG	16: 31,775,161 (GRCm39)		probably null	Het
Or5b119	A	T	19: 13,456,794 (GRCm39)	M256K	possibly damaging	Het
Or8g20	T	C	9: 39,395,614 (GRCm39)	T309A	probably benign	Het
Psmd12	A	G	11: 107,377,280 (GRCm39)	E113G	possibly damaging	Het
Ptpn3	T	C	4: 57,195,730 (GRCm39)	D879G	possibly damaging	Het
Rufy3	A	G	5: 88,785,166 (GRCm39)	Q414R	probably damaging	Het
Slc14a2	A	G	18: 78,197,317 (GRCm39)	I813T	probably damaging	Het
Smok2a	G	T	17: 13,444,500 (GRCm39)	A26S	probably benign	Het
Spsb3	T	A	17: 25,110,550 (GRCm39)	L459Q	probably damaging	Het
Tacc2	T	C	7: 130,225,142 (GRCm39)	V609A	possibly damaging	Het
Tmem185b	C	A	1: 119,454,365 (GRCm39)	A42E	probably damaging	Het
Trim56	A	G	5: 137,141,470 (GRCm39)	V682A	probably damaging	Het
Trim75	T	C	8: 65,435,488 (GRCm39)	K321E	possibly damaging	Het
Ttc17	A	C	2: 94,133,891 (GRCm39)	M1098R	possibly damaging	Het
Ydjc	C	A	16: 16,965,545 (GRCm39)	H136Q	probably damaging	Het
Zfp568	A	T	7: 29,716,682 (GRCm39)	H193L	probably benign	Het
Znhit1	A	G	5: 137,011,254 (GRCm39)	V153A	probably benign	Het

Other mutations in Mrgprx1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01152:Mrgprx1	APN	7	47,671,234 (GRCm39)	missense	probably benign	0.00
IGL01326:Mrgprx1	APN	7	47,671,517 (GRCm39)	missense	probably benign	0.26
IGL02117:Mrgprx1	APN	7	47,671,371 (GRCm39)	nonsense	probably null
IGL02219:Mrgprx1	APN	7	47,671,477 (GRCm39)	missense	probably benign	0.20
IGL02431:Mrgprx1	APN	7	47,670,875 (GRCm39)	missense	probably benign	0.00
IGL02441:Mrgprx1	APN	7	47,671,336 (GRCm39)	missense	probably benign	0.39
IGL02682:Mrgprx1	APN	7	47,671,740 (GRCm39)	missense	probably damaging	1.00
R0219:Mrgprx1	UTSW	7	47,671,294 (GRCm39)	missense	probably damaging	1.00
R4366:Mrgprx1	UTSW	7	47,670,941 (GRCm39)	missense	probably damaging	0.98
R4521:Mrgprx1	UTSW	7	47,671,447 (GRCm39)	missense	probably benign
R4801:Mrgprx1	UTSW	7	47,670,959 (GRCm39)	missense	possibly damaging	0.89
R4802:Mrgprx1	UTSW	7	47,670,959 (GRCm39)	missense	possibly damaging	0.89
R5452:Mrgprx1	UTSW	7	47,671,556 (GRCm39)	missense	probably benign	0.07
R5537:Mrgprx1	UTSW	7	47,670,898 (GRCm39)	missense	probably benign
R6834:Mrgprx1	UTSW	7	47,671,385 (GRCm39)	missense	probably damaging	0.99
R7406:Mrgprx1	UTSW	7	47,671,733 (GRCm39)	missense	possibly damaging	0.62
R9664:Mrgprx1	UTSW	7	47,671,273 (GRCm39)	missense	probably benign	0.38
RF020:Mrgprx1	UTSW	7	47,671,259 (GRCm39)	small insertion	probably benign
RF024:Mrgprx1	UTSW	7	47,671,259 (GRCm39)	small insertion	probably benign
RF026:Mrgprx1	UTSW	7	47,671,257 (GRCm39)	small insertion	probably benign
RF043:Mrgprx1	UTSW	7	47,671,257 (GRCm39)	small insertion	probably benign
Z1088:Mrgprx1	UTSW	7	47,670,877 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TTGGCCACAATACACACAGG -3'
(R):5'- TGTGAGCATGGATCCAACCATC -3'

Sequencing Primer
(F):5'- TACACACAGGCAGCGCTCTG -3'
(R):5'- TGAGCATGGATCCAACCATCTCATC -3'

Posted On

2018-05-24