Mutagenetix > Incidental Mutation

Incidental Mutation 'R6456:Cyp4a29'

520159

Institutional Source

Beutler Lab

Gene Symbol

Cyp4a29

Ensembl Gene

ENSMUSG00000083138

Gene Name

cytochrome P450, family 4, subfamily a, polypeptide 29

Synonyms

Cyp4a29-ps

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R6456 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

115099281-115111754 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 115108381 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 368 (M368I)

Ref Sequence

ENSEMBL: ENSMUSP00000139717 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000118278]

AlphaFold

A0A087WPC3

Predicted Effect

probably benign
Transcript: ENSMUST00000118278
AA Change: M368I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000139717
Gene: ENSMUSG00000083138
AA Change: M368I

Domain	Start	End	E-Value	Type
transmembrane domain	9	31	N/A	INTRINSIC
Pfam:p450	51	504	1.1e-127	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.4%
20x: 91.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	T	11: 9,240,474 (GRCm39)	H779L	possibly damaging	Het
Abca7	T	C	10: 79,850,984 (GRCm39)	V2097A	probably null	Het
Adam8	A	G	7: 139,566,701 (GRCm39)	S524P	possibly damaging	Het
Anapc2	T	C	2: 25,170,207 (GRCm39)	M575T	probably damaging	Het
Arhgap42	T	A	9: 9,005,823 (GRCm39)	I736L	probably benign	Het
AU040320	A	G	4: 126,736,284 (GRCm39)	N789S	probably benign	Het
Bmi1	A	G	2: 18,687,058 (GRCm39)	Y46C	probably damaging	Het
Brd10	G	A	19: 29,693,914 (GRCm39)	P1860S	possibly damaging	Het
Ccdc125	T	C	13: 100,832,817 (GRCm39)	S465P	possibly damaging	Het
Cd180	C	T	13: 102,839,344 (GRCm39)	L76F	probably damaging	Het
Cep135	T	C	5: 76,739,571 (GRCm39)		probably benign	Het
Col6a5	G	A	9: 105,822,676 (GRCm39)	T227I	unknown	Het
Ddx28	T	A	8: 106,737,000 (GRCm39)	I353F	possibly damaging	Het
Dhx40	G	A	11: 86,675,800 (GRCm39)	T198M	probably damaging	Het
Dnai4	A	C	4: 102,906,746 (GRCm39)	M689R	probably benign	Het
Dync2i2	T	C	2: 29,922,779 (GRCm39)	S323G	probably benign	Het
Fat4	G	A	3: 39,038,128 (GRCm39)	V3927M	possibly damaging	Het
Garin1b	A	G	6: 29,334,045 (GRCm39)	N299S	probably benign	Het
Gm10226	G	T	17: 21,910,932 (GRCm39)	G56*	probably null	Het
H2-T15	A	T	17: 36,367,502 (GRCm39)	Y279N	probably damaging	Het
Itsn2	T	G	12: 4,679,923 (GRCm39)		probably benign	Het
Lrrc8a	A	G	2: 30,145,486 (GRCm39)	D100G	probably benign	Het
Madd	T	C	2: 91,008,536 (GRCm39)	H122R	probably benign	Het
Mfsd4b3-ps	C	G	10: 39,823,316 (GRCm39)	V315L	probably benign	Het
Mki67	C	G	7: 135,301,204 (GRCm39)	A1277P	possibly damaging	Het
Nlrp9b	T	A	7: 19,782,703 (GRCm39)	N872K	probably damaging	Het
Npas1	T	C	7: 16,195,851 (GRCm39)	T274A	probably benign	Het
Nrm	A	T	17: 36,176,292 (GRCm39)		probably null	Het
Or10j2	A	T	1: 173,098,105 (GRCm39)	D121V	probably damaging	Het
Pdilt	T	A	7: 119,099,706 (GRCm39)	L187F	probably damaging	Het
Pkdcc	T	C	17: 83,527,548 (GRCm39)	I242T	probably damaging	Het
Plch2	C	A	4: 155,077,459 (GRCm39)	D535Y	probably damaging	Het
Pmpca	T	A	2: 26,285,179 (GRCm39)	I468N	probably damaging	Het
Prpf4	T	C	4: 62,332,869 (GRCm39)		probably null	Het
Rcc1	A	G	4: 132,061,427 (GRCm39)	S361P	probably benign	Het
Rigi	T	A	4: 40,213,838 (GRCm39)	N607Y	possibly damaging	Het
Rnf213	A	G	11: 119,350,792 (GRCm39)	I3876V	probably benign	Het
Sall2	T	A	14: 52,551,050 (GRCm39)	Q715L	probably damaging	Het
Sall2	G	A	14: 52,551,051 (GRCm39)	Q713*	probably null	Het
Sin3a	A	G	9: 57,020,985 (GRCm39)	S1004G	possibly damaging	Het
Sltm	C	T	9: 70,450,269 (GRCm39)	T23M	probably damaging	Het
Spata31e2	T	A	1: 26,724,250 (GRCm39)	H310L	probably damaging	Het
Sspo	A	G	6: 48,428,740 (GRCm39)	E385G	probably benign	Het
Syne3	G	T	12: 104,906,963 (GRCm39)	R775S	possibly damaging	Het
Szt2	A	T	4: 118,233,894 (GRCm39)		probably benign	Het
Tlk2	T	C	11: 105,112,099 (GRCm39)	S151P	probably benign	Het
Trabd2b	C	T	4: 114,443,757 (GRCm39)	R305C	probably damaging	Het
Ttc21b	T	C	2: 66,018,675 (GRCm39)	Q1244R	probably damaging	Het
Vmn2r125	A	G	4: 156,703,357 (GRCm39)	N245S	probably benign	Het
Wdr64	G	A	1: 175,613,175 (GRCm39)		probably null	Het
Wdr70	G	A	15: 7,915,118 (GRCm39)	T550M	possibly damaging	Het

Other mutations in Cyp4a29

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02687:Cyp4a29	APN	4	115,108,397 (GRCm39)	missense	probably damaging	0.99
IGL03224:Cyp4a29	APN	4	115,104,247 (GRCm39)	missense	probably damaging	0.97
IGL03271:Cyp4a29	APN	4	115,111,705 (GRCm39)	missense	probably damaging	1.00
IGL03387:Cyp4a29	APN	4	115,108,368 (GRCm39)	missense	possibly damaging	0.70
R0304:Cyp4a29	UTSW	4	115,110,129 (GRCm39)	splice site	probably benign
R2656:Cyp4a29	UTSW	4	115,106,921 (GRCm39)	missense	possibly damaging	0.95
R4012:Cyp4a29	UTSW	4	115,105,707 (GRCm39)	missense	probably benign
R4834:Cyp4a29	UTSW	4	115,106,867 (GRCm39)	missense	probably benign	0.00
R4856:Cyp4a29	UTSW	4	115,110,078 (GRCm39)	missense	probably benign
R4886:Cyp4a29	UTSW	4	115,110,078 (GRCm39)	missense	probably benign
R4939:Cyp4a29	UTSW	4	115,104,873 (GRCm39)	critical splice donor site	probably null
R4967:Cyp4a29	UTSW	4	115,104,196 (GRCm39)	missense	probably benign	0.00
R5071:Cyp4a29	UTSW	4	115,104,860 (GRCm39)	missense	probably benign	0.00
R5072:Cyp4a29	UTSW	4	115,104,860 (GRCm39)	missense	probably benign	0.00
R5073:Cyp4a29	UTSW	4	115,104,860 (GRCm39)	missense	probably benign	0.00
R5620:Cyp4a29	UTSW	4	115,108,088 (GRCm39)	missense	probably benign	0.12
R5818:Cyp4a29	UTSW	4	115,104,229 (GRCm39)	missense	possibly damaging	0.89
R6219:Cyp4a29	UTSW	4	115,106,927 (GRCm39)	missense	probably damaging	0.99
R6318:Cyp4a29	UTSW	4	115,107,396 (GRCm39)	missense	probably benign	0.00
R6386:Cyp4a29	UTSW	4	115,104,272 (GRCm39)	critical splice donor site	probably null
R7393:Cyp4a29	UTSW	4	115,099,393 (GRCm39)	missense	probably damaging	1.00
R7443:Cyp4a29	UTSW	4	115,105,756 (GRCm39)	missense	probably damaging	1.00
R7719:Cyp4a29	UTSW	4	115,108,137 (GRCm39)	missense	possibly damaging	0.65
R7831:Cyp4a29	UTSW	4	115,107,367 (GRCm39)	missense	probably benign	0.00
R7983:Cyp4a29	UTSW	4	115,108,099 (GRCm39)	missense	probably damaging	1.00
R8304:Cyp4a29	UTSW	4	115,111,653 (GRCm39)	missense	probably damaging	1.00
R8674:Cyp4a29	UTSW	4	115,106,882 (GRCm39)	missense	probably benign	0.08
R9109:Cyp4a29	UTSW	4	115,108,395 (GRCm39)	missense	probably damaging	1.00
R9298:Cyp4a29	UTSW	4	115,108,395 (GRCm39)	missense	probably damaging	1.00
R9486:Cyp4a29	UTSW	4	115,106,916 (GRCm39)	missense	probably damaging	1.00
R9601:Cyp4a29	UTSW	4	115,105,772 (GRCm39)	missense	probably damaging	0.98
R9667:Cyp4a29	UTSW	4	115,111,630 (GRCm39)	missense	probably damaging	1.00
R9790:Cyp4a29	UTSW	4	115,108,380 (GRCm39)	missense	probably damaging	1.00
R9791:Cyp4a29	UTSW	4	115,108,380 (GRCm39)	missense	probably damaging	1.00
U24488:Cyp4a29	UTSW	4	115,108,204 (GRCm39)	missense	possibly damaging	0.95
Z1088:Cyp4a29	UTSW	4	115,105,693 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TTTGGCTACACATCCCGACC -3'
(R):5'- TCTGCACAGATTTACTTACAAGGAG -3'

Sequencing Primer
(F):5'- CCACCAGCAGAGATGCAGAG -3'
(R):5'- GCACACAAAAAGATCCGATTTTTC -3'

Posted On

2018-06-06