Mutagenetix > Incidental Mutation

Incidental Mutation 'R6536:Polr3g'

520431

Institutional Source

Beutler Lab

Gene Symbol

Polr3g

Ensembl Gene

ENSMUSG00000035834

Gene Name

polymerase (RNA) III (DNA directed) polypeptide G

Synonyms

RPC32, 2310047G20Rik

MMRRC Submission

044662-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6536 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

81821962-81859132 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 81826335 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 162 (N162S)

Ref Sequence

ENSEMBL: ENSMUSP00000035289 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048993]

AlphaFold

Q6NXY9

Predicted Effect

unknown
Transcript: ENSMUST00000048993
AA Change: N162S

SMART Domains

Protein: ENSMUSP00000035289
Gene: ENSMUSG00000035834
AA Change: N162S

Domain	Start	End	E-Value	Type
Pfam:RNA_pol_3_Rpc31	1	223	7e-41	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162535

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.4%

Validation Efficiency

100% (36/36)

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930111J21Rik2	G	T	11: 48,910,550 (GRCm39)	H628N	probably benign	Het
Abcb1a	T	A	5: 8,769,030 (GRCm39)	F751I	probably benign	Het
Adamts13	G	A	2: 26,865,762 (GRCm39)	V106M	probably damaging	Het
Add1	C	A	5: 34,758,780 (GRCm39)	N31K	possibly damaging	Het
Adgrf5	G	T	17: 43,733,552 (GRCm39)		probably benign	Het
Akap11	T	G	14: 78,748,754 (GRCm39)	D1211A	possibly damaging	Het
Atp5f1c	G	A	2: 10,085,127 (GRCm39)		probably benign	Het
Cd320	T	C	17: 34,066,477 (GRCm39)	S72P	probably benign	Het
Clca4b	C	A	3: 144,622,490 (GRCm39)	W525L	possibly damaging	Het
Cripto	A	T	9: 110,773,257 (GRCm39)		probably null	Het
Csmd3	G	A	15: 47,701,863 (GRCm39)	T1740I	probably damaging	Het
Dnah7c	T	G	1: 46,697,450 (GRCm39)	S2122A	probably benign	Het
Enpp2	T	C	15: 54,726,027 (GRCm39)	N583S	probably damaging	Het
Fcsk	A	G	8: 111,610,511 (GRCm39)	V964A	possibly damaging	Het
Gpd2	A	T	2: 57,235,367 (GRCm39)	I366F	probably benign	Het
Hsd17b2	G	A	8: 118,428,921 (GRCm39)	V63M	possibly damaging	Het
Hsdl2	A	T	4: 59,610,508 (GRCm39)		probably null	Het
Idh3b	AG	AGCACCACAACTG	2: 130,121,593 (GRCm39)		probably null	Het
Ifi44	A	G	3: 151,438,126 (GRCm39)	V387A	probably benign	Het
Kcnc4	T	C	3: 107,355,512 (GRCm39)	D312G	possibly damaging	Het
Kif1b	T	C	4: 149,277,053 (GRCm39)	M1337V	probably benign	Het
Klra6	C	T	6: 130,000,682 (GRCm39)	V41I	probably benign	Het
Lrp1	A	G	10: 127,393,937 (GRCm39)		probably null	Het
Mpdz	T	C	4: 81,301,654 (GRCm39)	E257G	probably damaging	Het
Or10v1	T	C	19: 11,873,760 (GRCm39)	V125A	probably benign	Het
Or4f14	T	C	2: 111,743,119 (GRCm39)	D52G	possibly damaging	Het
Papln	A	G	12: 83,828,661 (GRCm39)	Y789C	probably damaging	Het
Pcdh15	T	C	10: 74,467,221 (GRCm39)	L1680P	probably damaging	Het
Pcdhac1	A	G	18: 37,223,367 (GRCm39)	N60S	probably benign	Het
Pou3f3	A	G	1: 42,737,374 (GRCm39)	I357V	probably damaging	Het
Sycp2	A	G	2: 177,993,441 (GRCm39)	S1235P	probably damaging	Het
Tns3	A	T	11: 8,384,531 (GRCm39)	V1429E	probably damaging	Het
Trim67	T	C	8: 125,521,081 (GRCm39)	S148P	possibly damaging	Het
Usp49	A	T	17: 47,990,617 (GRCm39)	I348F	probably damaging	Het
Wac	A	T	18: 7,905,189 (GRCm39)		probably null	Het
Zfp775	A	G	6: 48,596,543 (GRCm39)	K139R	probably damaging	Het

Other mutations in Polr3g

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00886:Polr3g	APN	13	81,842,796 (GRCm39)	missense	probably damaging	1.00
IGL01764:Polr3g	APN	13	81,830,238 (GRCm39)	missense	possibly damaging	0.93
R0118:Polr3g	UTSW	13	81,824,240 (GRCm39)	unclassified	probably benign
R0550:Polr3g	UTSW	13	81,842,892 (GRCm39)	missense	probably damaging	0.99
R3725:Polr3g	UTSW	13	81,842,754 (GRCm39)	missense	probably damaging	0.99
R6684:Polr3g	UTSW	13	81,847,650 (GRCm39)	splice site	probably null
R7645:Polr3g	UTSW	13	81,842,563 (GRCm39)	missense	unknown
R8296:Polr3g	UTSW	13	81,842,682 (GRCm39)	missense	unknown
R8317:Polr3g	UTSW	13	81,826,302 (GRCm39)	missense	unknown
R9178:Polr3g	UTSW	13	81,842,535 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TCCACTGTACTCGTTAACGTGAAG -3'
(R):5'- GGCTGGTAATCCCGTAATTTAGG -3'

Sequencing Primer
(F):5'- CGTGAAGTATTTATCAACTCAACTCC -3'
(R):5'- CCCGTAATTTAGGATTCAGCGAG -3'

Posted On

2018-06-06