Mutagenetix > Incidental Mutation

Incidental Mutation 'R6540:Mroh9'

520657

Institutional Source

Beutler Lab

Gene Symbol

Mroh9

Ensembl Gene

ENSMUSG00000071890

Gene Name

maestro heat-like repeat family member 9

Synonyms

4921528O07Rik, Armc11

MMRRC Submission

044666-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6540 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

162851871-162913239 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 162866541 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Proline at position 701 (T701P)

Ref Sequence

ENSEMBL: ENSMUSP00000094365 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096608]

AlphaFold

G5E8L9

Predicted Effect

possibly damaging
Transcript: ENSMUST00000096608
AA Change: T701P

PolyPhen 2 Score 0.866 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000094365
Gene: ENSMUSG00000071890
AA Change: T701P

Domain	Start	End	E-Value	Type
SCOP:d1gw5b_	231	716	2e-8	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000195132

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A3galt2	T	C	4: 128,660,779 (GRCm39)	Y143H	possibly damaging	Het
Aco1	G	A	4: 40,186,367 (GRCm39)	R593Q	probably benign	Het
Adamts2	T	C	11: 50,679,567 (GRCm39)	V849A	possibly damaging	Het
Ash1l	A	T	3: 88,892,368 (GRCm39)	T1416S	probably damaging	Het
Cdc5l	A	G	17: 45,737,570 (GRCm39)	W63R	probably damaging	Het
Cep95	T	A	11: 106,692,328 (GRCm39)	D169E	probably damaging	Het
Col14a1	A	C	15: 55,235,977 (GRCm39)	N297T	unknown	Het
Cp	T	C	3: 20,018,693 (GRCm39)		probably null	Het
Fbxl19	G	T	7: 127,347,525 (GRCm39)		probably benign	Het
Flnc	T	C	6: 29,446,376 (GRCm39)	V931A	possibly damaging	Het
Foxj2	A	G	6: 122,810,202 (GRCm39)	H187R	probably benign	Het
Hectd4	G	A	5: 121,441,634 (GRCm39)	V1199I	probably benign	Het
Ighv7-4	A	G	12: 114,186,470 (GRCm39)	Y101H	possibly damaging	Het
Igkv10-94	T	A	6: 68,681,507 (GRCm39)	Y111F	probably benign	Het
Lin28a	C	T	4: 133,745,372 (GRCm39)	V74M	possibly damaging	Het
Lrrc49	T	C	9: 60,592,335 (GRCm39)	N53S	possibly damaging	Het
Ndst4	A	T	3: 125,515,801 (GRCm39)	K309*	probably null	Het
Nlrp9a	T	C	7: 26,256,817 (GRCm39)	V145A	possibly damaging	Het
Or2l5	T	C	16: 19,333,571 (GRCm39)	I272V	probably benign	Het
Ovgp1	A	T	3: 105,893,897 (GRCm39)	K557*	probably null	Het
Pde4b	T	C	4: 102,459,073 (GRCm39)	L546P	probably damaging	Het
Pkd1	G	A	17: 24,794,951 (GRCm39)	V2213M	probably damaging	Het
Plekhh1	A	G	12: 79,111,263 (GRCm39)	I542V	probably benign	Het
Ppan	A	G	9: 20,802,506 (GRCm39)		probably null	Het
Prdm15	A	T	16: 97,637,005 (GRCm39)	V104E	probably benign	Het
Semp2l2a	A	C	8: 13,887,573 (GRCm39)	S173A	probably benign	Het
Sik3	A	G	9: 46,123,351 (GRCm39)	H1050R	probably benign	Het
Slc35d3	A	G	10: 19,725,086 (GRCm39)	F257L	possibly damaging	Het
Tfip11	A	G	5: 112,482,263 (GRCm39)		probably null	Het
Tmem132d	A	G	5: 128,345,596 (GRCm39)	S309P	possibly damaging	Het
Tmem87a	T	C	2: 120,234,400 (GRCm39)	I48V	probably benign	Het
V1rd19	T	A	7: 23,703,056 (GRCm39)	L174*	probably null	Het
Zfp729a	A	G	13: 67,767,767 (GRCm39)	C821R	possibly damaging	Het
Zfp970	C	A	2: 177,167,388 (GRCm39)	H321N	probably damaging	Het
Zzef1	T	C	11: 72,804,055 (GRCm39)	L2599P	probably damaging	Het

Other mutations in Mroh9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Mroh9	APN	1	162,873,350 (GRCm39)	missense	possibly damaging	0.89
IGL00705:Mroh9	APN	1	162,907,072 (GRCm39)	missense	probably damaging	1.00
IGL00788:Mroh9	APN	1	162,852,227 (GRCm39)	missense	probably benign	0.06
IGL00795:Mroh9	APN	1	162,888,191 (GRCm39)	missense	probably damaging	1.00
IGL00815:Mroh9	APN	1	162,866,700 (GRCm39)	missense	probably damaging	1.00
IGL01025:Mroh9	APN	1	162,875,435 (GRCm39)	missense	possibly damaging	0.67
IGL01303:Mroh9	APN	1	162,908,144 (GRCm39)	missense	probably benign	0.00
IGL01526:Mroh9	APN	1	162,883,172 (GRCm39)	missense	probably damaging	0.99
IGL01680:Mroh9	APN	1	162,875,551 (GRCm39)	splice site	probably null
IGL01823:Mroh9	APN	1	162,883,178 (GRCm39)	missense	probably benign	0.39
IGL02024:Mroh9	APN	1	162,890,071 (GRCm39)	missense	possibly damaging	0.65
IGL02213:Mroh9	APN	1	162,885,648 (GRCm39)	missense	probably damaging	1.00
IGL02455:Mroh9	APN	1	162,903,149 (GRCm39)	missense	probably benign	0.03
IGL02546:Mroh9	APN	1	162,908,145 (GRCm39)	missense	probably benign	0.04
IGL03059:Mroh9	APN	1	162,852,205 (GRCm39)	missense	possibly damaging	0.95
IGL03061:Mroh9	APN	1	162,854,071 (GRCm39)	missense	probably damaging	1.00
IGL03071:Mroh9	APN	1	162,866,766 (GRCm39)	missense	probably damaging	1.00
R0048:Mroh9	UTSW	1	162,890,056 (GRCm39)	missense	probably damaging	0.97
R0048:Mroh9	UTSW	1	162,890,056 (GRCm39)	missense	probably damaging	0.97
R0441:Mroh9	UTSW	1	162,888,331 (GRCm39)	missense	probably damaging	1.00
R0506:Mroh9	UTSW	1	162,888,205 (GRCm39)	missense	possibly damaging	0.90
R0629:Mroh9	UTSW	1	162,888,205 (GRCm39)	missense	possibly damaging	0.90
R0751:Mroh9	UTSW	1	162,893,693 (GRCm39)	missense	possibly damaging	0.84
R1301:Mroh9	UTSW	1	162,871,552 (GRCm39)	critical splice donor site	probably null
R1481:Mroh9	UTSW	1	162,854,078 (GRCm39)	missense	probably damaging	1.00
R1618:Mroh9	UTSW	1	162,852,110 (GRCm39)	missense	probably benign	0.00
R1647:Mroh9	UTSW	1	162,873,625 (GRCm39)	missense	probably damaging	1.00
R1648:Mroh9	UTSW	1	162,873,625 (GRCm39)	missense	probably damaging	1.00
R1668:Mroh9	UTSW	1	162,852,161 (GRCm39)	missense	possibly damaging	0.52
R1795:Mroh9	UTSW	1	162,884,347 (GRCm39)	missense	probably damaging	0.97
R1796:Mroh9	UTSW	1	162,873,279 (GRCm39)	missense	probably damaging	1.00
R1857:Mroh9	UTSW	1	162,866,714 (GRCm39)	missense	probably damaging	0.98
R1869:Mroh9	UTSW	1	162,854,082 (GRCm39)	missense	probably damaging	0.97
R1923:Mroh9	UTSW	1	162,903,860 (GRCm39)	missense	probably damaging	1.00
R2325:Mroh9	UTSW	1	162,854,099 (GRCm39)	splice site	probably null
R2511:Mroh9	UTSW	1	162,866,514 (GRCm39)	missense	probably benign	0.13
R2912:Mroh9	UTSW	1	162,871,572 (GRCm39)	missense	probably damaging	1.00
R2913:Mroh9	UTSW	1	162,871,572 (GRCm39)	missense	probably damaging	1.00
R2919:Mroh9	UTSW	1	162,884,341 (GRCm39)	missense	probably damaging	1.00
R2973:Mroh9	UTSW	1	162,884,338 (GRCm39)	missense	probably damaging	1.00
R3912:Mroh9	UTSW	1	162,893,638 (GRCm39)	missense	probably damaging	0.97
R4034:Mroh9	UTSW	1	162,908,122 (GRCm39)	critical splice donor site	probably null
R4551:Mroh9	UTSW	1	162,871,662 (GRCm39)	missense	probably damaging	0.98
R4656:Mroh9	UTSW	1	162,893,593 (GRCm39)	missense	probably damaging	1.00
R4662:Mroh9	UTSW	1	162,883,162 (GRCm39)	missense	probably damaging	0.97
R4743:Mroh9	UTSW	1	162,852,061 (GRCm39)	missense	probably benign	0.05
R4890:Mroh9	UTSW	1	162,854,093 (GRCm39)	missense	probably damaging	1.00
R5128:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5129:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5147:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5157:Mroh9	UTSW	1	162,871,690 (GRCm39)	missense	probably damaging	0.96
R5324:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5325:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5340:Mroh9	UTSW	1	162,908,156 (GRCm39)	start gained	probably benign
R6005:Mroh9	UTSW	1	162,903,246 (GRCm39)	missense	probably damaging	0.99
R6182:Mroh9	UTSW	1	162,893,612 (GRCm39)	nonsense	probably null
R6414:Mroh9	UTSW	1	162,902,271 (GRCm39)	missense	probably damaging	1.00
R6477:Mroh9	UTSW	1	162,903,873 (GRCm39)	missense	probably damaging	1.00
R6541:Mroh9	UTSW	1	162,885,607 (GRCm39)	missense	possibly damaging	0.78
R6643:Mroh9	UTSW	1	162,903,130 (GRCm39)	missense	probably damaging	1.00
R6811:Mroh9	UTSW	1	162,873,610 (GRCm39)	missense	possibly damaging	0.86
R6830:Mroh9	UTSW	1	162,903,935 (GRCm39)	missense	probably benign
R7026:Mroh9	UTSW	1	162,888,251 (GRCm39)	missense	probably benign	0.00
R7052:Mroh9	UTSW	1	162,866,525 (GRCm39)	missense	possibly damaging	0.92
R7068:Mroh9	UTSW	1	162,866,750 (GRCm39)	missense	probably damaging	1.00
R7350:Mroh9	UTSW	1	162,903,858 (GRCm39)	critical splice donor site	probably null
R7545:Mroh9	UTSW	1	162,902,277 (GRCm39)	missense	possibly damaging	0.56
R7615:Mroh9	UTSW	1	162,873,601 (GRCm39)	missense	probably benign	0.40
R7743:Mroh9	UTSW	1	162,852,122 (GRCm39)	missense	probably benign
R7808:Mroh9	UTSW	1	162,866,678 (GRCm39)	missense	probably damaging	1.00
R8024:Mroh9	UTSW	1	162,866,802 (GRCm39)	missense	probably benign	0.02
R8062:Mroh9	UTSW	1	162,866,544 (GRCm39)	missense	probably damaging	1.00
R8145:Mroh9	UTSW	1	162,890,096 (GRCm39)	missense	probably benign	0.00
R8426:Mroh9	UTSW	1	162,852,294 (GRCm39)	missense	probably damaging	0.98
R8458:Mroh9	UTSW	1	162,883,250 (GRCm39)	missense	probably damaging	1.00
R8555:Mroh9	UTSW	1	162,899,595 (GRCm39)	splice site	probably null
R8960:Mroh9	UTSW	1	162,883,196 (GRCm39)	missense	probably benign	0.25
R9040:Mroh9	UTSW	1	162,890,069 (GRCm39)	missense	probably benign	0.06
R9125:Mroh9	UTSW	1	162,875,412 (GRCm39)	missense	probably benign	0.19
R9154:Mroh9	UTSW	1	162,890,030 (GRCm39)	missense
R9596:Mroh9	UTSW	1	162,893,576 (GRCm39)	missense	probably damaging	0.98
R9612:Mroh9	UTSW	1	162,866,498 (GRCm39)	missense	probably damaging	1.00
RF003:Mroh9	UTSW	1	162,885,630 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TACCCTGTGGCTTGAAACCC -3'
(R):5'- ACATAGTCCTAGGAAGCCTCG -3'

Sequencing Primer
(F):5'- GTACCACTACAACATGAGGAATTG -3'
(R):5'- AAGCCTCGTGCCCCTGATC -3'

Posted On

2018-06-06