|Institutional Source||Beutler Lab|
|Gene Name||argonaute RISC catalytic subunit 4|
|Synonyms||argonaute 4, Eif2c4, 5730550L01Rik|
|Is this an essential gene?||Possibly non essential (E-score: 0.488)|
|Stock #||R6545 (G1)|
|Chromosomal Location||126489541-126533472 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to A at 126512018 bp|
|Amino Acid Change||Glutamine to Leucine at position 366 (Q366L)|
|Ref Sequence||ENSEMBL: ENSMUSP00000081312 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000084289]|
|Predicted Effect||probably benign
AA Change: Q366L
PolyPhen 2 Score 0.104 (Sensitivity: 0.93; Specificity: 0.86)
AA Change: Q366L
|Predicted Effect||noncoding transcript
|Coding Region Coverage||
|Validation Efficiency||95% (42/44)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the Argonaute family of proteins which contain PAZ and PIWI domains and play an integral role in RNA interference and short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of related family members. [provided by RefSeq, Mar 2017]
PHENOTYPE: Male mice homozygous for a null mutation display oligozoospermia, decreased testis weight, premature entry into meiosis and disruption of sex body formation. However both males and females are fertile. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ago4||
(F):5'- AGCTCTGTCATCTCGTTGTG -3'
(R):5'- GTTCCTGGAAATAGACAAAGACTTC -3'
(F):5'- CCAAATTCCTTCAGGTATGGGTCAG -3'
(R):5'- GACTTCATGTATAGAAGCCCATTTC -3'