Mutagenetix > Incidental Mutation

Incidental Mutation 'R6545:Wdr97'

521144

Institutional Source

Beutler Lab

Gene Symbol

Wdr97

Ensembl Gene

ENSMUSG00000109179

Gene Name

WD repeat domain 97

Synonyms

Gm35339

MMRRC Submission

044671-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.146) question?

Stock #

R6545 (G1)

Quality Score

137.008

Status

Validated

Chromosome

Chromosomal Location

76238649-76249141 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 76247578 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Cysteine at position 1585 (R1585C)

Ref Sequence

ENSEMBL: ENSMUSP00000146420 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000208833]

AlphaFold

A0A140LHH9

Predicted Effect

probably damaging
Transcript: ENSMUST00000208833
AA Change: R1585C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.6%

Validation Efficiency

95% (42/44)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ago1	C	T	4: 126,348,145 (GRCm39)	A58T	possibly damaging	Het
Ago4	T	A	4: 126,405,811 (GRCm39)	Q366L	probably benign	Het
Card10	C	A	15: 78,661,010 (GRCm39)	G950V	probably damaging	Het
Ceacam1	A	T	7: 25,173,279 (GRCm39)	V303D	probably damaging	Het
Cfap54	C	T	10: 92,672,319 (GRCm39)	R2917H	probably benign	Het
Cit	A	G	5: 115,984,493 (GRCm39)	S22G	probably null	Het
Cog4	A	G	8: 111,607,577 (GRCm39)	E666G	probably damaging	Het
Crocc2	A	G	1: 93,140,659 (GRCm39)	D1103G	probably benign	Het
Cttnbp2	A	T	6: 18,405,278 (GRCm39)		probably null	Het
Dctn3	T	C	4: 41,723,084 (GRCm39)	E16G	probably damaging	Het
Dnah6	A	G	6: 73,021,715 (GRCm39)	S3536P	probably damaging	Het
Eef2	T	A	10: 81,016,948 (GRCm39)	I675N	probably damaging	Het
Gga3	A	G	11: 115,477,995 (GRCm39)	F531S	possibly damaging	Het
Gm19410	A	G	8: 36,257,652 (GRCm39)	R697G	possibly damaging	Het
Gm3173	T	A	14: 15,728,395 (GRCm39)	M18K	possibly damaging	Het
Gm5800	A	T	14: 51,949,419 (GRCm39)	S175R	possibly damaging	Het
Gria2	T	C	3: 80,648,451 (GRCm39)	K95R	probably damaging	Het
Gstm6	A	G	3: 107,849,681 (GRCm39)	I76T	probably damaging	Het
Harbi1	T	G	2: 91,542,640 (GRCm39)	Y34D	probably damaging	Het
Hectd2	C	A	19: 36,564,778 (GRCm39)	Q20K	probably benign	Het
Inpp5f	C	A	7: 128,296,280 (GRCm39)	A250D	possibly damaging	Het
Irf9	T	C	14: 55,842,684 (GRCm39)	F59L	probably damaging	Het
Itgam	T	A	7: 127,707,044 (GRCm39)	M625K	probably damaging	Het
Kcnh5	C	T	12: 75,054,432 (GRCm39)	R504Q	probably damaging	Het
Lama2	T	C	10: 27,052,793 (GRCm39)	T1389A	probably benign	Het
Lin54	T	A	5: 100,632,996 (GRCm39)		probably null	Het
Mettl23	A	G	11: 116,740,042 (GRCm39)	D171G	possibly damaging	Het
Mgll	A	G	6: 88,802,685 (GRCm39)	N296S	probably benign	Het
Mpv17	T	A	5: 31,302,041 (GRCm39)		probably benign	Het
Myof	A	T	19: 37,930,745 (GRCm39)	M1001K	possibly damaging	Het
Myom1	A	T	17: 71,389,300 (GRCm39)	Q850L	probably benign	Het
Or10ab5	A	T	7: 108,245,662 (GRCm39)	N40K	probably damaging	Het
Pias2	A	G	18: 77,217,781 (GRCm39)	I328V	possibly damaging	Het
Polh	G	A	17: 46,493,685 (GRCm39)	P311S	possibly damaging	Het
Prss34	A	T	17: 25,517,809 (GRCm39)	R61S	probably benign	Het
Rassf2	A	T	2: 131,840,237 (GRCm39)	M280K	probably damaging	Het
Rpsa	A	G	9: 119,959,323 (GRCm39)	H47R	probably benign	Het
Rtp3	A	G	9: 110,815,894 (GRCm39)	V219A	possibly damaging	Het
Smarcc2	T	C	10: 128,319,997 (GRCm39)	I790T	probably benign	Het
Stag3	A	G	5: 138,296,614 (GRCm39)	T491A	possibly damaging	Het
Svil	C	T	18: 5,108,621 (GRCm39)	H2007Y	probably benign	Het
Togaram1	T	G	12: 65,024,981 (GRCm39)	C750G	possibly damaging	Het
Vmn2r13	A	T	5: 109,304,806 (GRCm39)		probably null	Het
Vmn2r4	A	T	3: 64,313,777 (GRCm39)	D401E	possibly damaging	Het
Wsb1	A	T	11: 79,141,881 (GRCm39)	D45E	probably damaging	Het
Zfp808	T	A	13: 62,319,709 (GRCm39)	Y313N	probably benign	Het

Other mutations in Wdr97

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R6006:Wdr97	UTSW	15	76,241,372 (GRCm39)	missense	probably damaging	1.00
R6258:Wdr97	UTSW	15	76,239,895 (GRCm39)	nonsense	probably null
R6464:Wdr97	UTSW	15	76,246,977 (GRCm39)	missense	probably benign	0.01
R6544:Wdr97	UTSW	15	76,242,478 (GRCm39)	missense	probably benign	0.00
R6554:Wdr97	UTSW	15	76,239,178 (GRCm39)	missense	possibly damaging	0.64
R6803:Wdr97	UTSW	15	76,240,776 (GRCm39)	missense	probably damaging	1.00
R6850:Wdr97	UTSW	15	76,241,996 (GRCm39)	missense	probably damaging	1.00
R7574:Wdr97	UTSW	15	76,241,949 (GRCm39)	nonsense	probably null
R7793:Wdr97	UTSW	15	76,243,307 (GRCm39)	missense
R7985:Wdr97	UTSW	15	76,245,687 (GRCm39)	missense
R8008:Wdr97	UTSW	15	76,244,832 (GRCm39)	missense
R8170:Wdr97	UTSW	15	76,247,819 (GRCm39)	missense
R8171:Wdr97	UTSW	15	76,247,819 (GRCm39)	missense
R8267:Wdr97	UTSW	15	76,240,794 (GRCm39)	missense
R8337:Wdr97	UTSW	15	76,245,684 (GRCm39)	missense
R8478:Wdr97	UTSW	15	76,247,629 (GRCm39)	splice site	probably null
R8735:Wdr97	UTSW	15	76,240,775 (GRCm39)	missense
R8958:Wdr97	UTSW	15	76,245,694 (GRCm39)	missense
R9385:Wdr97	UTSW	15	76,240,367 (GRCm39)	missense
R9440:Wdr97	UTSW	15	76,245,064 (GRCm39)	missense
R9657:Wdr97	UTSW	15	76,245,476 (GRCm39)	missense
R9672:Wdr97	UTSW	15	76,240,377 (GRCm39)	missense
R9778:Wdr97	UTSW	15	76,241,125 (GRCm39)	missense
RF004:Wdr97	UTSW	15	76,247,373 (GRCm39)	frame shift	probably null
RF009:Wdr97	UTSW	15	76,247,367 (GRCm39)	frame shift	probably null
RF016:Wdr97	UTSW	15	76,240,172 (GRCm39)	missense
RF030:Wdr97	UTSW	15	76,247,365 (GRCm39)	frame shift	probably null
Z1177:Wdr97	UTSW	15	76,247,330 (GRCm39)	missense
Z1177:Wdr97	UTSW	15	76,239,130 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- ACAGCAGCAGAGATCATCGATG -3'
(R):5'- GCTCTGCATCTTGTGACCTG -3'

Sequencing Primer
(F):5'- GAAGAGGAGCATTTACCTGTCTCAC -3'
(R):5'- TGACCTGGATGTGGGAAGCC -3'

Posted On

2018-06-06