Mutagenetix > Incidental Mutation

Incidental Mutation 'R6525:Vmn2r81'

521723

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r81

Ensembl Gene

ENSMUSG00000055515

Gene Name

vomeronasal 2, receptor 81

Synonyms

pheromone recepter, V2rf2, EC1-VR2

MMRRC Submission

044651-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R6525 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

79083611-79130369 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 79129560 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 817 (M817K)

Ref Sequence

ENSEMBL: ENSMUSP00000020547 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020547]

AlphaFold

Q80Z09

Predicted Effect

probably benign
Transcript: ENSMUST00000020547
AA Change: M817K

PolyPhen 2 Score 0.106 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000020547
Gene: ENSMUSG00000055515
AA Change: M817K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	79	474	7.7e-37	PFAM
Pfam:NCD3G	517	570	8.9e-21	PFAM
Pfam:7tm_3	603	838	6.1e-50	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.8%
20x: 93.0%

Validation Efficiency

96% (71/74)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit vomeronasal sensory neuron axons that spread out diffusely over the posterior accessory olfactory bulb and extend abnormally into the anterior olfactory bulb. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	A	G	3: 121,931,308 (GRCm39)	E1447G	probably benign	Het
Acss2	A	G	2: 155,392,337 (GRCm39)	N261S	probably benign	Het
Adcy8	C	A	15: 64,609,243 (GRCm39)	G859*	probably null	Het
Agbl3	A	T	6: 34,780,529 (GRCm39)	K496*	probably null	Het
Antxrl	A	T	14: 33,782,363 (GRCm39)	D182V	probably damaging	Het
Arid5b	A	G	10: 67,933,496 (GRCm39)	L559P	possibly damaging	Het
Azi2	T	A	9: 117,876,663 (GRCm39)	S60T	probably damaging	Het
Bahcc1	T	C	11: 120,176,048 (GRCm39)	Y1931H	probably damaging	Het
Cnga1	T	A	5: 72,775,574 (GRCm39)	E49V	probably damaging	Het
Col3a1	A	G	1: 45,386,339 (GRCm39)	N160D	possibly damaging	Het
Crem	C	A	18: 3,268,070 (GRCm39)	R267L	probably damaging	Het
Ddx52	T	C	11: 83,844,145 (GRCm39)		probably null	Het
Ddx6	T	C	9: 44,534,926 (GRCm39)	I127T	probably damaging	Het
Dop1b	T	A	16: 93,606,304 (GRCm39)	Y2094N	probably damaging	Het
Dst	C	A	1: 34,202,216 (GRCm39)	N181K	probably damaging	Het
Dusp7	A	G	9: 106,246,483 (GRCm39)	K163E	possibly damaging	Het
Dynlt1a	T	A	17: 6,362,014 (GRCm39)	T55S	probably benign	Het
Enpp2	T	C	15: 54,733,607 (GRCm39)	N451S	probably benign	Het
Faap100	C	A	11: 120,269,590 (GRCm39)		probably null	Het
Fam53a	T	C	5: 33,765,262 (GRCm39)	N148S	probably damaging	Het
Fat2	T	C	11: 55,174,626 (GRCm39)	D2029G	probably damaging	Het
Flcn	A	T	11: 59,684,998 (GRCm39)	N484K	possibly damaging	Het
Gbp10	T	A	5: 105,383,950 (GRCm39)	E17D	probably benign	Het
Gm14226	T	C	2: 154,867,003 (GRCm39)	V320A	possibly damaging	Het
Gna13	T	C	11: 109,286,765 (GRCm39)	I196T	probably damaging	Het
Gorasp1	G	T	9: 119,757,061 (GRCm39)	P374T	possibly damaging	Het
Hc	T	C	2: 34,881,236 (GRCm39)	D1461G	probably benign	Het
Hmcn1	T	C	1: 150,573,317 (GRCm39)	N2111D	probably damaging	Het
Hs3st3b1	G	A	11: 63,812,424 (GRCm39)	S97L	probably benign	Het
Hsdl2	A	G	4: 59,612,696 (GRCm39)	T296A	probably damaging	Het
Impg2	A	G	16: 56,025,512 (GRCm39)	D48G	probably damaging	Het
Kbtbd12	A	T	6: 88,591,062 (GRCm39)	N383K	probably benign	Het
Kcnj12	T	G	11: 60,960,397 (GRCm39)	F232V	probably damaging	Het
Krt1	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	15: 101,758,813 (GRCm39)		probably benign	Homo
Ldhb	T	C	6: 142,436,191 (GRCm39)	D326G	probably benign	Het
Lrrc49	A	G	9: 60,505,432 (GRCm39)	L607S	probably damaging	Het
Ltn1	A	G	16: 87,217,074 (GRCm39)	S388P	probably damaging	Het
Mansc4	A	G	6: 146,976,645 (GRCm39)	S324P	probably benign	Het
Meltf	G	A	16: 31,707,717 (GRCm39)	W368*	probably null	Het
Nacad	A	G	11: 6,552,255 (GRCm39)	L312P	probably damaging	Het
Ndc1	G	T	4: 107,225,304 (GRCm39)	G7W	probably benign	Het
Nmral1	T	A	16: 4,532,296 (GRCm39)	K172*	probably null	Het
Nol9	G	T	4: 152,123,906 (GRCm39)	R32L	probably damaging	Het
Nsun5	A	G	5: 135,403,912 (GRCm39)	Y296C	probably damaging	Het
Or4k51	C	T	2: 111,585,329 (GRCm39)	T245I	probably benign	Het
Oscp1	A	G	4: 125,970,571 (GRCm39)	D120G	possibly damaging	Het
Parp14	C	T	16: 35,680,811 (GRCm39)	C274Y	probably benign	Het
Pced1b	T	A	15: 97,282,679 (GRCm39)	H239Q	possibly damaging	Het
Pgap1	T	C	1: 54,521,048 (GRCm39)	I865V	probably benign	Het
Ppp6r3	T	A	19: 3,543,936 (GRCm39)	S360C	probably damaging	Het
Prb1a	A	G	6: 132,184,467 (GRCm39)	S389P	unknown	Het
Prr16	T	G	18: 51,436,227 (GRCm39)	S235R	probably benign	Het
Rab11fip1	T	C	8: 27,646,527 (GRCm39)	N183S	probably benign	Het
Rcn1	A	G	2: 105,219,320 (GRCm39)		probably null	Het
Rimkla	C	A	4: 119,325,288 (GRCm39)	A374S	probably benign	Het
Skint8	A	T	4: 111,785,935 (GRCm39)	D127V	probably damaging	Het
Slc12a6	A	G	2: 112,182,796 (GRCm39)	K724E	probably damaging	Het
Slc13a3	T	C	2: 165,248,667 (GRCm39)	N537S	unknown	Het
Slc26a5	T	C	5: 22,025,348 (GRCm39)	D457G	possibly damaging	Het
Slx4ip	T	C	2: 136,842,138 (GRCm39)	V21A	possibly damaging	Het
Stt3b	A	G	9: 115,087,626 (GRCm39)	Y291H	probably damaging	Het
Syn3	G	A	10: 86,302,916 (GRCm39)	P80S	probably damaging	Het
Tasor2	G	A	13: 3,626,540 (GRCm39)	Q455*	probably null	Het
Tiam1	A	T	16: 89,655,485 (GRCm39)		probably null	Het
Tjp1	T	C	7: 64,993,399 (GRCm39)	D58G	probably damaging	Het
Tmprss15	T	A	16: 78,800,266 (GRCm39)	I621F	probably damaging	Het
Tns1	C	T	1: 73,992,629 (GRCm39)	S683N	probably damaging	Het
Ttn	A	G	2: 76,773,436 (GRCm39)	L2322P	probably damaging	Het
Ugdh	G	T	5: 65,574,402 (GRCm39)	H409N	probably damaging	Het
Wwc1	C	T	11: 35,744,264 (GRCm39)	E853K	probably benign	Het
Zbtb34	A	G	2: 33,302,145 (GRCm39)	V132A	probably damaging	Het
Zfp119b	A	G	17: 56,246,992 (GRCm39)	C33R	possibly damaging	Het

Other mutations in Vmn2r81

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01111:Vmn2r81	APN	10	79,083,831 (GRCm39)	missense	probably benign	0.20
IGL02255:Vmn2r81	APN	10	79,083,806 (GRCm39)	nonsense	probably null
IGL02901:Vmn2r81	APN	10	79,106,564 (GRCm39)	missense	probably damaging	0.98
R0383:Vmn2r81	UTSW	10	79,129,281 (GRCm39)	missense	possibly damaging	0.95
R0555:Vmn2r81	UTSW	10	79,129,283 (GRCm39)	missense	probably damaging	1.00
R0715:Vmn2r81	UTSW	10	79,104,434 (GRCm39)	missense	probably damaging	1.00
R0967:Vmn2r81	UTSW	10	79,083,857 (GRCm39)	splice site	probably benign
R1438:Vmn2r81	UTSW	10	79,129,691 (GRCm39)	missense	probably benign	0.00
R1468:Vmn2r81	UTSW	10	79,129,496 (GRCm39)	missense	probably damaging	1.00
R1468:Vmn2r81	UTSW	10	79,129,496 (GRCm39)	missense	probably damaging	1.00
R1589:Vmn2r81	UTSW	10	79,128,858 (GRCm39)	missense	probably damaging	0.99
R1671:Vmn2r81	UTSW	10	79,103,265 (GRCm39)	missense	probably benign	0.04
R1672:Vmn2r81	UTSW	10	79,104,112 (GRCm39)	missense	probably damaging	0.99
R1728:Vmn2r81	UTSW	10	79,106,489 (GRCm39)	missense	probably benign	0.08
R1784:Vmn2r81	UTSW	10	79,106,489 (GRCm39)	missense	probably benign	0.08
R1931:Vmn2r81	UTSW	10	79,129,328 (GRCm39)	missense	probably damaging	0.98
R1934:Vmn2r81	UTSW	10	79,083,628 (GRCm39)	start codon destroyed	probably null	0.71
R1944:Vmn2r81	UTSW	10	79,129,571 (GRCm39)	missense	probably damaging	1.00
R2102:Vmn2r81	UTSW	10	79,129,334 (GRCm39)	missense	probably damaging	0.97
R2190:Vmn2r81	UTSW	10	79,104,085 (GRCm39)	missense	possibly damaging	0.67
R2392:Vmn2r81	UTSW	10	79,110,516 (GRCm39)	missense	probably damaging	1.00
R3770:Vmn2r81	UTSW	10	79,106,434 (GRCm39)	missense	probably damaging	1.00
R4551:Vmn2r81	UTSW	10	79,104,241 (GRCm39)	missense	possibly damaging	0.95
R4629:Vmn2r81	UTSW	10	79,103,276 (GRCm39)	missense	probably damaging	0.99
R4691:Vmn2r81	UTSW	10	79,129,211 (GRCm39)	nonsense	probably null
R4829:Vmn2r81	UTSW	10	79,083,635 (GRCm39)	missense	possibly damaging	0.48
R4914:Vmn2r81	UTSW	10	79,106,357 (GRCm39)	missense	probably null	1.00
R4996:Vmn2r81	UTSW	10	79,129,247 (GRCm39)	missense	probably benign	0.04
R5242:Vmn2r81	UTSW	10	79,129,309 (GRCm39)	nonsense	probably null
R5253:Vmn2r81	UTSW	10	79,083,820 (GRCm39)	missense	probably benign	0.00
R5925:Vmn2r81	UTSW	10	79,083,637 (GRCm39)	missense	probably damaging	1.00
R6169:Vmn2r81	UTSW	10	79,104,382 (GRCm39)	missense	probably benign
R6270:Vmn2r81	UTSW	10	79,129,649 (GRCm39)	missense	probably benign
R6813:Vmn2r81	UTSW	10	79,104,439 (GRCm39)	missense	probably benign	0.00
R6886:Vmn2r81	UTSW	10	79,103,988 (GRCm39)	missense	possibly damaging	0.90
R7200:Vmn2r81	UTSW	10	79,106,570 (GRCm39)	critical splice donor site	probably null
R7350:Vmn2r81	UTSW	10	79,104,219 (GRCm39)	missense	probably benign	0.03
R7504:Vmn2r81	UTSW	10	79,104,166 (GRCm39)	missense	probably benign
R7832:Vmn2r81	UTSW	10	79,129,664 (GRCm39)	missense	probably damaging	1.00
R8130:Vmn2r81	UTSW	10	79,110,538 (GRCm39)	missense	possibly damaging	0.95
R8362:Vmn2r81	UTSW	10	79,103,283 (GRCm39)	missense	probably benign	0.17
R8407:Vmn2r81	UTSW	10	79,104,028 (GRCm39)	missense	possibly damaging	0.92
R8540:Vmn2r81	UTSW	10	79,129,065 (GRCm39)	missense	probably damaging	0.99
R8558:Vmn2r81	UTSW	10	79,106,467 (GRCm39)	missense	possibly damaging	0.94
R8700:Vmn2r81	UTSW	10	79,129,517 (GRCm39)	missense	probably damaging	1.00
R8779:Vmn2r81	UTSW	10	79,103,218 (GRCm39)	missense	possibly damaging	0.78
R8987:Vmn2r81	UTSW	10	79,129,704 (GRCm39)	missense	probably damaging	1.00
R9001:Vmn2r81	UTSW	10	79,104,104 (GRCm39)	missense	possibly damaging	0.73
R9055:Vmn2r81	UTSW	10	79,110,441 (GRCm39)	missense	probably benign
R9145:Vmn2r81	UTSW	10	79,104,028 (GRCm39)	missense	possibly damaging	0.92
R9151:Vmn2r81	UTSW	10	79,103,905 (GRCm39)	missense
R9253:Vmn2r81	UTSW	10	79,129,582 (GRCm39)	missense	probably damaging	1.00
R9255:Vmn2r81	UTSW	10	79,103,166 (GRCm39)	missense	possibly damaging	0.77
R9370:Vmn2r81	UTSW	10	79,104,424 (GRCm39)	missense	probably damaging	1.00
R9588:Vmn2r81	UTSW	10	79,129,205 (GRCm39)	missense	possibly damaging	0.46
X0067:Vmn2r81	UTSW	10	79,128,877 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- ATCATTCTTTGCAACAAGGGCTC -3'
(R):5'- TGCCATAACTTTACATTCGACAGTC -3'

Sequencing Primer
(F):5'- ACTCTGGCTTACCTGGGAGTC -3'
(R):5'- GACAGTCAAACTGGTTGAG -3'

Posted On

2018-06-06