Mutagenetix > Incidental Mutation

Incidental Mutation 'R6527:Or11j4'

522026

Institutional Source

Beutler Lab

Gene Symbol

Or11j4

Ensembl Gene

ENSMUSG00000047716

Gene Name

olfactory receptor family 11 subfamily J member 4

Synonyms

GA_x6K02T2PMLR-6089963-6090901, MOR106-5, Olfr736

MMRRC Submission

044653-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R6527 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

50630215-50631153 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 50630885 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 224 (L224*)

Ref Sequence

ENSEMBL: ENSMUSP00000149654 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058965] [ENSMUST00000213402] [ENSMUST00000213755] [ENSMUST00000215227] [ENSMUST00000215263]

AlphaFold

Q8VFT6

Predicted Effect

probably null
Transcript: ENSMUST00000058965
AA Change: L224*

SMART Domains

Protein: ENSMUSP00000062700
Gene: ENSMUSG00000047716
AA Change: L224*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	311	1.5e-45	PFAM
Pfam:7TM_GPCR_Srsx	37	175	6.9e-7	PFAM
Pfam:7tm_1	43	294	4.7e-15	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000213402
AA Change: L224*

Predicted Effect

probably null
Transcript: ENSMUST00000213755
AA Change: L224*

Predicted Effect

probably null
Transcript: ENSMUST00000215227
AA Change: L224*

Predicted Effect

probably null
Transcript: ENSMUST00000215263
AA Change: L224*

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.7%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 137,773,288 (GRCm39)	T826A	probably damaging	Het
Abca16	T	A	7: 120,076,995 (GRCm39)	I686N	possibly damaging	Het
Abcb6	T	C	1: 75,154,132 (GRCm39)		probably null	Het
Adgrl3	A	C	5: 81,935,364 (GRCm39)	E1299A	probably damaging	Het
Amd2	A	C	10: 35,586,802 (GRCm39)	Y252D	probably damaging	Het
Cfap74	A	G	4: 155,506,722 (GRCm39)		probably null	Het
Dhx29	G	T	13: 113,069,076 (GRCm39)	K135N	probably damaging	Het
Dlg5	T	A	14: 24,240,516 (GRCm39)	D245V	possibly damaging	Het
Dscaml1	C	T	9: 45,623,482 (GRCm39)	Q83*	probably null	Het
Dsp	T	A	13: 38,379,849 (GRCm39)	L1599Q	probably damaging	Het
Duox2	A	G	2: 122,125,095 (GRCm39)	V369A	probably benign	Het
Flacc1	T	C	1: 58,731,572 (GRCm39)	M1V	probably null	Het
Gbe1	T	A	16: 70,230,560 (GRCm39)		probably null	Het
Gm7298	A	G	6: 121,746,669 (GRCm39)	K600R	probably benign	Het
Heatr4	C	T	12: 84,026,537 (GRCm39)	G240E	probably damaging	Het
Jakmip2	A	G	18: 43,689,589 (GRCm39)	V651A	possibly damaging	Het
Jam3	C	T	9: 27,066,640 (GRCm39)	R8Q	unknown	Het
Letm2	A	G	8: 26,082,522 (GRCm39)		probably benign	Het
Lmod3	T	C	6: 97,224,339 (GRCm39)	D494G	probably benign	Het
Mast2	T	C	4: 116,172,136 (GRCm39)	D604G	probably damaging	Het
Mif4gd	C	T	11: 115,500,101 (GRCm39)		probably null	Het
Msr1	T	C	8: 40,077,274 (GRCm39)	E112G	possibly damaging	Het
Mtus2	A	G	5: 148,214,408 (GRCm39)		probably null	Het
Muc4	A	G	16: 32,753,433 (GRCm38)	H1103R	probably benign	Het
Nudt14	T	A	12: 112,898,507 (GRCm39)	I198F	possibly damaging	Het
Or13p3	T	A	4: 118,567,045 (GRCm39)	F147Y	possibly damaging	Het
Or1n1b	T	C	2: 36,780,594 (GRCm39)	T89A	probably benign	Het
Osbpl8	T	C	10: 111,129,066 (GRCm39)	I884T	probably benign	Het
Podxl2	T	C	6: 88,819,912 (GRCm39)	N550S	probably damaging	Het
Ppp1r9a	A	G	6: 5,045,949 (GRCm39)	Y151C	probably damaging	Het
Prss42	T	C	9: 110,629,924 (GRCm39)	V226A	possibly damaging	Het
Psmd12	A	G	11: 107,379,794 (GRCm39)	I116V	probably damaging	Het
Psme4	A	C	11: 30,782,175 (GRCm39)	I872L	probably benign	Het
Rab11fip1	A	T	8: 27,664,420 (GRCm39)	V65E	probably damaging	Het
Ros1	T	C	10: 52,019,473 (GRCm39)	N700S	possibly damaging	Het
Slc15a4	G	A	5: 127,673,773 (GRCm39)	T547M	probably damaging	Het
Slfn3	T	A	11: 83,103,932 (GRCm39)	C268S	probably benign	Het
Smc5	T	C	19: 23,205,554 (GRCm39)	Q794R	probably benign	Het
Sqor	A	G	2: 122,651,206 (GRCm39)	Y434C	probably damaging	Het
Steap4	T	A	5: 8,028,502 (GRCm39)	L360H	probably damaging	Het
Sycp1	A	G	3: 102,806,203 (GRCm39)	V496A	probably benign	Het
Tmem161b	T	G	13: 84,420,383 (GRCm39)	M128R	probably benign	Het
Tmem59l	T	C	8: 70,938,775 (GRCm39)	E102G	probably damaging	Het
Tnks	A	T	8: 35,340,247 (GRCm39)	V457D	probably benign	Het
Tomt	A	G	7: 101,549,599 (GRCm39)	Y230H	probably damaging	Het
Trim37	A	G	11: 87,080,910 (GRCm39)	N561D	probably damaging	Het
V1ra8	T	A	6: 90,180,295 (GRCm39)	I166K	probably damaging	Het
Vmn1r56	A	G	7: 5,199,575 (GRCm39)	V14A	probably benign	Het
Vsig8	T	C	1: 172,387,925 (GRCm39)	V5A	possibly damaging	Het
Vwa8	A	T	14: 79,184,653 (GRCm39)	S384C	possibly damaging	Het
Wwc1	C	T	11: 35,744,264 (GRCm39)	E853K	probably benign	Het
Zfp984	T	C	4: 147,840,381 (GRCm39)	N157D	probably benign	Het
Zzef1	G	A	11: 72,765,816 (GRCm39)	D1448N	probably benign	Het

Other mutations in Or11j4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01765:Or11j4	APN	14	50,630,291 (GRCm39)	missense	probably benign	0.33
IGL01952:Or11j4	APN	14	50,630,860 (GRCm39)	missense	probably benign	0.02
IGL01996:Or11j4	APN	14	50,631,116 (GRCm39)	missense	probably damaging	0.99
IGL02694:Or11j4	APN	14	50,630,257 (GRCm39)	missense	probably benign	0.02
IGL02717:Or11j4	APN	14	50,631,104 (GRCm39)	missense	probably damaging	1.00
IGL03185:Or11j4	APN	14	50,630,855 (GRCm39)	missense	probably damaging	0.99
IGL03218:Or11j4	APN	14	50,631,115 (GRCm39)	missense	probably damaging	0.98
IGL03048:Or11j4	UTSW	14	50,630,245 (GRCm39)	missense	possibly damaging	0.47
R0066:Or11j4	UTSW	14	50,630,659 (GRCm39)	missense	probably benign	0.08
R0066:Or11j4	UTSW	14	50,630,659 (GRCm39)	missense	probably benign	0.08
R0089:Or11j4	UTSW	14	50,630,321 (GRCm39)	missense	probably benign
R0254:Or11j4	UTSW	14	50,630,536 (GRCm39)	missense	probably damaging	0.99
R0284:Or11j4	UTSW	14	50,630,452 (GRCm39)	missense	probably damaging	1.00
R1800:Or11j4	UTSW	14	50,630,786 (GRCm39)	nonsense	probably null
R3885:Or11j4	UTSW	14	50,630,326 (GRCm39)	missense	probably benign	0.05
R4302:Or11j4	UTSW	14	50,630,903 (GRCm39)	missense	probably benign	0.23
R4452:Or11j4	UTSW	14	50,630,369 (GRCm39)	missense	probably benign
R4705:Or11j4	UTSW	14	50,630,257 (GRCm39)	missense	probably benign	0.02
R5340:Or11j4	UTSW	14	50,630,677 (GRCm39)	missense	probably damaging	0.98
R6007:Or11j4	UTSW	14	50,630,948 (GRCm39)	missense	probably damaging	1.00
R6338:Or11j4	UTSW	14	50,630,857 (GRCm39)	missense	possibly damaging	0.47
R6358:Or11j4	UTSW	14	50,630,845 (GRCm39)	missense	possibly damaging	0.78
R6521:Or11j4	UTSW	14	50,631,005 (GRCm39)	missense	possibly damaging	0.95
R6777:Or11j4	UTSW	14	50,631,115 (GRCm39)	missense	probably damaging	0.98
R6903:Or11j4	UTSW	14	50,631,089 (GRCm39)	missense	possibly damaging	0.48
R8899:Or11j4	UTSW	14	50,630,269 (GRCm39)	missense	probably damaging	0.99
X0026:Or11j4	UTSW	14	50,630,998 (GRCm39)	missense	possibly damaging	0.79

Predicted Primers

PCR Primer
(F):5'- TGATACTCATTACTCAGCTGCC -3'
(R):5'- TCGGAGGCTATAGATCATGGGG -3'

Sequencing Primer
(F):5'- CCATTTTGTGGGCCAAATGTGATAG -3'
(R):5'- GGCTATAGATCATGGGGTTGAAG -3'

Posted On

2018-06-06