Incidental Mutation 'R6529:Zfp664'
ID 522171
Institutional Source Beutler Lab
Gene Symbol Zfp664
Ensembl Gene ENSMUSG00000079215
Gene Name zinc finger protein 664
Synonyms D930038J03Rik
MMRRC Submission 044655-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6529 (G1)
Quality Score 225.009
Status Validated
Chromosome 5
Chromosomal Location 124939758-124965694 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to T at 124963352 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Histidine to Tyrosine at position 249 (H249Y)
Ref Sequence ENSEMBL: ENSMUSP00000107048 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000111417]
AlphaFold no structure available at present
Predicted Effect probably damaging
Transcript: ENSMUST00000111417
AA Change: H249Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000107048
Gene: ENSMUSG00000079215
AA Change: H249Y

DomainStartEndE-ValueType
ZnF_C2H2 3 25 2.57e-3 SMART
ZnF_C2H2 31 53 7.55e-1 SMART
ZnF_C2H2 59 81 5.99e-4 SMART
ZnF_C2H2 87 109 3.21e-4 SMART
ZnF_C2H2 115 137 4.54e-4 SMART
ZnF_C2H2 143 165 4.79e-3 SMART
ZnF_C2H2 171 193 8.34e-3 SMART
ZnF_C2H2 199 221 6.23e-2 SMART
ZnF_C2H2 227 249 7.9e-4 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000130616
Predicted Effect noncoding transcript
Transcript: ENSMUST00000134463
Predicted Effect noncoding transcript
Transcript: ENSMUST00000134473
Predicted Effect noncoding transcript
Transcript: ENSMUST00000141533
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.4%
  • 20x: 91.6%
Validation Efficiency 98% (41/42)
Allele List at MGI
Other mutations in this stock
Total: 40 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acan A T 7: 78,739,479 (GRCm39) M296L probably benign Het
Atxn2 T C 5: 121,949,677 (GRCm39) probably null Het
B3galnt2 G T 13: 14,170,377 (GRCm39) R242S probably benign Het
Bltp3a A G 17: 28,098,750 (GRCm39) I218M possibly damaging Het
Casz1 G A 4: 149,022,646 (GRCm39) E571K probably damaging Het
Ccdc163 A G 4: 116,566,121 (GRCm39) probably null Het
Cd109 A G 9: 78,619,907 (GRCm39) D1383G probably damaging Het
Cd200r1 A G 16: 44,610,065 (GRCm39) T95A possibly damaging Het
Chd2 T C 7: 73,153,191 (GRCm39) E219G possibly damaging Het
Cibar1 A G 4: 12,168,978 (GRCm39) V175A probably damaging Het
Dnah14 T A 1: 181,494,034 (GRCm39) V1730D probably damaging Het
Eps8 G A 6: 137,491,335 (GRCm39) H348Y possibly damaging Het
Fbxo2 A T 4: 148,249,511 (GRCm39) D187V probably damaging Het
Fsip2 A T 2: 82,812,657 (GRCm39) Y2992F probably benign Het
Gle1 A T 2: 29,825,539 (GRCm39) T10S possibly damaging Het
Got2 T C 8: 96,615,013 (GRCm39) probably benign Het
Gtf3c6 T C 10: 40,127,251 (GRCm39) T34A probably benign Het
H4c11 G T 13: 21,919,476 (GRCm39) V71F possibly damaging Het
Klf15 C T 6: 90,444,394 (GRCm39) T323I probably damaging Het
Krtap5-3 C A 7: 141,756,079 (GRCm39) C305* probably null Het
Map2k6 A T 11: 110,383,388 (GRCm39) D99V probably damaging Het
Nckap5l G T 15: 99,324,475 (GRCm39) P676Q probably benign Het
Nup188 A T 2: 30,216,466 (GRCm39) T757S possibly damaging Het
Or10ak13 C T 4: 118,638,907 (GRCm39) V292I probably benign Het
Or51q1c T C 7: 103,653,133 (GRCm39) V217A probably benign Het
Peg3 C A 7: 6,711,071 (GRCm39) A1384S probably damaging Het
Plekho2 C T 9: 65,480,383 (GRCm39) R14H probably benign Het
Qsox2 A T 2: 26,107,753 (GRCm39) C247S probably damaging Het
Slc25a13 A G 6: 6,073,451 (GRCm39) V469A probably benign Het
Slitrk3 T C 3: 72,958,551 (GRCm39) T74A probably benign Het
Spmip7 T C 11: 11,465,009 (GRCm39) F120S possibly damaging Het
Sult1a1 T C 7: 126,274,310 (GRCm39) T91A probably benign Het
Sult3a2 T C 10: 33,655,733 (GRCm39) Y82C probably damaging Het
Taf1b A T 12: 24,606,650 (GRCm39) H490L possibly damaging Het
Trrap T A 5: 144,771,014 (GRCm39) H2804Q probably benign Het
Usp8 A G 2: 126,567,298 (GRCm39) I106V probably benign Het
Wdcp T A 12: 4,901,143 (GRCm39) V333D probably damaging Het
Wdr46 T A 17: 34,168,120 (GRCm39) L564Q possibly damaging Het
Wrn T C 8: 33,826,004 (GRCm39) probably null Het
Zfp975 A C 7: 42,311,325 (GRCm39) H429Q possibly damaging Het
Other mutations in Zfp664
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01556:Zfp664 APN 5 124,963,252 (GRCm39) nonsense probably null
IGL02683:Zfp664 APN 5 124,963,386 (GRCm39) missense probably benign 0.03
R0394:Zfp664 UTSW 5 124,963,129 (GRCm39) nonsense probably null
R0629:Zfp664 UTSW 5 124,962,659 (GRCm39) missense probably damaging 0.99
R1400:Zfp664 UTSW 5 124,963,217 (GRCm39) missense unknown
R6052:Zfp664 UTSW 5 124,963,250 (GRCm39) missense unknown
R6058:Zfp664 UTSW 5 124,963,042 (GRCm39) nonsense probably null
R7031:Zfp664 UTSW 5 124,963,070 (GRCm39) missense probably benign 0.06
R7752:Zfp664 UTSW 5 124,962,839 (GRCm39) nonsense probably null
R7901:Zfp664 UTSW 5 124,962,839 (GRCm39) nonsense probably null
R8434:Zfp664 UTSW 5 124,962,827 (GRCm39) missense possibly damaging 0.90
R8686:Zfp664 UTSW 5 124,963,133 (GRCm39) missense possibly damaging 0.61
Predicted Primers PCR Primer
(F):5'- TCTGCATCCACCAGAGAGTG -3'
(R):5'- TCCATCCTGGGCAAATACAG -3'

Sequencing Primer
(F):5'- CACCAGAGAGTGCACACAGG -3'
(R):5'- TCCATCCTGGGCAAATACAGAGAAG -3'
Posted On 2018-06-06