Mutagenetix > Incidental Mutation

Incidental Mutation 'R6564:Or7g33'

522344

Institutional Source

Beutler Lab

Gene Symbol

Or7g33

Ensembl Gene

ENSMUSG00000051160

Gene Name

olfactory receptor family 7 subfamily G member 33

Synonyms

MOR154-1, GA_x6K02T2PVTD-13277703-13276786, Olfr853

MMRRC Submission

044688-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R6564 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19448307-19449224 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 19448506 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 240 (T240I)

Ref Sequence

ENSEMBL: ENSMUSP00000150773 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058296] [ENSMUST00000213834]

AlphaFold

Q8VGG6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000058296
AA Change: T240I

PolyPhen 2 Score 0.946 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000053262
Gene: ENSMUSG00000051160
AA Change: T240I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	305	1.3e-57	PFAM
Pfam:7TM_GPCR_Srsx	35	206	1.7e-7	PFAM
Pfam:7tm_1	41	290	1.1e-23	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212273

Predicted Effect

possibly damaging
Transcript: ENSMUST00000213834
AA Change: T240I

PolyPhen 2 Score 0.946 (Sensitivity: 0.80; Specificity: 0.95)

Meta Mutation Damage Score

0.1624

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.1%
20x: 96.9%

Validation Efficiency

98% (56/57)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4833420G17Rik	T	A	13: 119,622,613 (GRCm39)		probably null	Het
4931429L15Rik	C	A	9: 46,218,202 (GRCm39)	D179Y	probably damaging	Het
Abca1	A	T	4: 53,034,031 (GRCm39)	L2233Q	possibly damaging	Het
Abca12	T	A	1: 71,349,009 (GRCm39)	E834V	possibly damaging	Het
Abcc9	T	C	6: 142,548,834 (GRCm39)	Y1318C	probably damaging	Het
Adam15	C	A	3: 89,254,519 (GRCm39)	R121L	possibly damaging	Het
Afdn	T	A	17: 14,116,351 (GRCm39)	M1588K	probably benign	Het
Akap9	T	C	5: 4,078,491 (GRCm39)	S1849P	probably damaging	Het
Aoc1l1	T	C	6: 48,954,509 (GRCm39)	V549A	probably benign	Het
Bag6	T	C	17: 35,359,347 (GRCm39)	S213P	probably damaging	Het
Bpifb9a	T	C	2: 154,102,098 (GRCm39)	V65A	probably benign	Het
Chn1	T	A	2: 73,448,385 (GRCm39)	I203F	probably damaging	Het
Crnkl1	T	A	2: 145,770,165 (GRCm39)	E226V	possibly damaging	Het
Cspg4	A	G	9: 56,797,442 (GRCm39)	E1302G	probably benign	Het
Dnah5	G	T	15: 28,367,891 (GRCm39)	A2759S	probably benign	Het
Dtx1	T	A	5: 120,833,082 (GRCm39)	T119S	probably benign	Het
Enpp5	G	A	17: 44,396,155 (GRCm39)	G356S	probably damaging	Het
Ezr	C	T	17: 7,010,246 (GRCm39)	V268M	probably damaging	Het
Fbxl15	T	C	19: 46,317,777 (GRCm39)	V153A	probably damaging	Het
Fkbpl	C	A	17: 34,865,240 (GRCm39)	A336E	probably benign	Het
Gsg1l2	A	G	11: 67,677,330 (GRCm39)	T200A	possibly damaging	Het
H2ax	T	C	9: 44,246,209 (GRCm39)	Y51H	probably damaging	Het
Ifi213	A	C	1: 173,422,862 (GRCm39)	M1R	probably null	Het
Kcnt1	A	C	2: 25,801,063 (GRCm39)	D1045A	probably benign	Het
Kdm4a	C	A	4: 118,034,636 (GRCm39)	A32S	probably benign	Het
Klkb1	A	T	8: 45,726,671 (GRCm39)	V444E	probably damaging	Het
Mccc1	T	C	3: 36,030,825 (GRCm39)	T414A	probably damaging	Het
Miga1	A	C	3: 151,990,959 (GRCm39)	N367K	probably damaging	Het
Mmp7	A	G	9: 7,695,185 (GRCm39)	D49G	probably benign	Het
Nlrp1a	A	G	11: 71,014,398 (GRCm39)	L284P	probably damaging	Het
Or9s15	T	C	1: 92,524,285 (GRCm39)	S15P	probably benign	Het
Pbld1	T	A	10: 62,907,806 (GRCm39)	I224N	possibly damaging	Het
Pih1d1	A	T	7: 44,809,243 (GRCm39)	R276W	probably damaging	Het
Plekhg1	G	T	10: 3,914,153 (GRCm39)	V1292L	probably damaging	Het
Prex2	A	G	1: 11,171,285 (GRCm39)		probably null	Het
Rbm6	T	C	9: 107,710,697 (GRCm39)	Y498C	probably damaging	Het
Reps1	T	A	10: 17,998,140 (GRCm39)		probably null	Het
Ruvbl1	T	A	6: 88,456,208 (GRCm39)	I154N	possibly damaging	Het
Slc4a5	T	G	6: 83,257,042 (GRCm39)	F616V	possibly damaging	Het
Spag5	A	T	11: 78,206,401 (GRCm39)	T798S	probably damaging	Het
Spam1	T	A	6: 24,796,355 (GRCm39)	I102K	possibly damaging	Het
Sptbn2	C	A	19: 4,782,052 (GRCm39)	F430L	probably damaging	Het
Svs3a	T	A	2: 164,131,270 (GRCm39)	I21K	probably damaging	Het
Tet3	T	A	6: 83,363,052 (GRCm39)	I842L	possibly damaging	Het
Tlr2	T	A	3: 83,745,002 (GRCm39)	K360N	probably benign	Het
Tmem45b	C	G	9: 31,339,301 (GRCm39)	W138S	probably damaging	Het
Tpgs2	T	C	18: 25,291,344 (GRCm39)	E40G	probably damaging	Het
Traj40	T	C	14: 54,415,399 (GRCm39)		probably benign	Het
Trpm5	A	G	7: 142,626,507 (GRCm39)	S125P	probably damaging	Het
Ttc3	G	T	16: 94,243,470 (GRCm39)	C1158F	probably damaging	Het
Tubg1	T	C	11: 101,011,715 (GRCm39)	I74T	probably damaging	Het
Vav2	A	T	2: 27,169,197 (GRCm39)		probably null	Het
Wbp4	G	T	14: 79,704,868 (GRCm39)	H201N	probably damaging	Het
Wdhd1	A	G	14: 47,485,499 (GRCm39)	S821P	probably benign	Het
Wsb2	A	T	5: 117,508,625 (GRCm39)		probably null	Het
Zic5	A	G	14: 122,696,833 (GRCm39)	L594P	unknown	Het

Other mutations in Or7g33

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02074:Or7g33	APN	9	19,449,148 (GRCm39)	missense	possibly damaging	0.94
IGL02078:Or7g33	APN	9	19,448,749 (GRCm39)	missense	probably benign	0.14
IGL02092:Or7g33	APN	9	19,449,046 (GRCm39)	missense	probably damaging	0.99
IGL02728:Or7g33	APN	9	19,449,142 (GRCm39)	missense	possibly damaging	0.95
R0245:Or7g33	UTSW	9	19,448,408 (GRCm39)	missense	probably benign	0.01
R1331:Or7g33	UTSW	9	19,448,842 (GRCm39)	missense	probably benign	0.03
R1661:Or7g33	UTSW	9	19,448,624 (GRCm39)	missense	probably benign	0.00
R1696:Or7g33	UTSW	9	19,449,190 (GRCm39)	missense	probably damaging	1.00
R2004:Or7g33	UTSW	9	19,448,688 (GRCm39)	nonsense	probably null
R2182:Or7g33	UTSW	9	19,448,638 (GRCm39)	missense	probably benign	0.24
R3730:Or7g33	UTSW	9	19,448,447 (GRCm39)	missense	probably benign	0.01
R4002:Or7g33	UTSW	9	19,449,202 (GRCm39)	missense	probably benign	0.00
R4627:Or7g33	UTSW	9	19,448,969 (GRCm39)	missense	possibly damaging	0.86
R5027:Or7g33	UTSW	9	19,448,573 (GRCm39)	missense	probably damaging	1.00
R5486:Or7g33	UTSW	9	19,448,590 (GRCm39)	missense	probably benign
R5931:Or7g33	UTSW	9	19,448,629 (GRCm39)	missense	probably benign	0.38
R6229:Or7g33	UTSW	9	19,449,014 (GRCm39)	missense	possibly damaging	0.91
R6718:Or7g33	UTSW	9	19,448,495 (GRCm39)	missense	probably damaging	1.00
R7247:Or7g33	UTSW	9	19,448,629 (GRCm39)	missense	probably benign	0.38
R7347:Or7g33	UTSW	9	19,448,395 (GRCm39)	missense	probably damaging	0.98
R8437:Or7g33	UTSW	9	19,448,833 (GRCm39)	missense	probably benign	0.00
R8534:Or7g33	UTSW	9	19,448,605 (GRCm39)	missense	possibly damaging	0.78
R8904:Or7g33	UTSW	9	19,448,760 (GRCm39)	missense	possibly damaging	0.56
R9093:Or7g33	UTSW	9	19,448,914 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- GGCCTTGTTAGACATCAGTGGG -3'
(R):5'- TGGATGCCCTTCTACATGGC -3'

Sequencing Primer
(F):5'- GTAATTGGGGGAGCAACCTC -3'
(R):5'- CATGGCTTAATGACATTGCGTC -3'

Posted On

2018-06-06