Mutagenetix > Incidental Mutation

Incidental Mutation 'R6567:Vmn2r58'

522742

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r58

Ensembl Gene

ENSMUSG00000090383

Gene Name

vomeronasal 2, receptor 58

Synonyms

EG628422

MMRRC Submission

044691-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.377) question?

Stock #

R6567 (G1)

Quality Score

179.009

Status

Validated

Chromosome

Chromosomal Location

41486305-41522094 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 41514673 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 99 (T99A)

Ref Sequence

ENSEMBL: ENSMUSP00000126966 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171671]

AlphaFold

K7N6V2

Predicted Effect

probably benign
Transcript: ENSMUST00000171671
AA Change: T99A

PolyPhen 2 Score 0.338 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000126966
Gene: ENSMUSG00000090383
AA Change: T99A

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	8e-43	PFAM
Pfam:NCD3G	514	567	1.8e-23	PFAM
Pfam:7tm_3	597	835	2.9e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.0%

Validation Efficiency

100% (32/32)

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930111J21Rik1	T	C	11: 48,838,950 (GRCm39)	T546A	probably benign	Het
Ahnak	G	T	19: 8,986,170 (GRCm39)	V2485L	probably benign	Het
C2cd5	T	C	6: 142,976,974 (GRCm39)	I722M	possibly damaging	Het
Clca4b	A	G	3: 144,638,100 (GRCm39)	I54T	possibly damaging	Het
Dennd2c	C	T	3: 103,039,335 (GRCm39)	A161V	probably benign	Het
Dmxl1	T	C	18: 49,992,246 (GRCm39)	Y331H	probably damaging	Het
Dnaaf11	C	T	15: 66,310,228 (GRCm39)	V347I	probably benign	Het
Dnajc2	G	A	5: 21,971,676 (GRCm39)	R247W	probably damaging	Het
Dock3	T	C	9: 106,773,946 (GRCm39)	T380A	probably benign	Het
Evc2	T	C	5: 37,576,508 (GRCm39)	V1044A	probably benign	Het
Ints2	T	C	11: 86,117,487 (GRCm39)	H745R	probably benign	Het
Kcnh1	T	A	1: 191,959,412 (GRCm39)	M322K	probably benign	Het
Mmp19	A	G	10: 128,632,275 (GRCm39)	T191A	probably benign	Het
Mms19	A	G	19: 41,938,206 (GRCm39)		probably null	Het
Mtcl3	G	T	10: 29,023,279 (GRCm39)	V209F	probably benign	Het
Ncapd3	T	C	9: 26,978,300 (GRCm39)	I833T	possibly damaging	Het
Nif3l1	T	A	1: 58,494,789 (GRCm39)	C253S	probably benign	Het
Or52e2	A	T	7: 102,804,135 (GRCm39)	I273K	possibly damaging	Het
Pate5	T	A	9: 35,750,411 (GRCm39)	Y87F	probably benign	Het
Pcsk1	T	A	13: 75,278,189 (GRCm39)	I584N	probably damaging	Het
Pms2	T	C	5: 143,865,786 (GRCm39)	V50A	probably damaging	Het
Rptor	A	G	11: 119,786,838 (GRCm39)	I1268V	probably benign	Het
Scap	C	T	9: 110,212,630 (GRCm39)	R1021W	probably damaging	Het
Sos1	A	T	17: 80,740,932 (GRCm39)	Y618N	probably damaging	Het
Tesk2	C	T	4: 116,649,361 (GRCm39)	A157V	probably damaging	Het
Tm6sf2	C	A	8: 70,528,174 (GRCm39)	H108N	probably damaging	Het
Trank1	T	C	9: 111,176,589 (GRCm39)	V287A	probably benign	Het
Tsks	A	T	7: 44,603,305 (GRCm39)	Q369L	probably damaging	Het
Wbp11	C	T	6: 136,797,537 (GRCm39)	S294N	probably benign	Het
Zfp608	T	C	18: 55,030,628 (GRCm39)	Y1104C	probably damaging	Het
Zfp759	T	A	13: 67,287,150 (GRCm39)	S234T	probably benign	Het

Other mutations in Vmn2r58

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Vmn2r58	APN	7	41,513,854 (GRCm39)	missense	possibly damaging	0.53
IGL00924:Vmn2r58	APN	7	41,486,891 (GRCm39)	missense	probably damaging	1.00
IGL01291:Vmn2r58	APN	7	41,513,935 (GRCm39)	missense	probably benign	0.02
IGL01480:Vmn2r58	APN	7	41,514,116 (GRCm39)	missense	probably benign	0.01
IGL01551:Vmn2r58	APN	7	41,514,703 (GRCm39)	missense	probably damaging	0.97
IGL01591:Vmn2r58	APN	7	41,514,753 (GRCm39)	missense	probably benign	0.03
IGL01940:Vmn2r58	APN	7	41,487,071 (GRCm39)	missense	probably benign	0.00
IGL01994:Vmn2r58	APN	7	41,486,394 (GRCm39)	missense	probably damaging	0.98
IGL02041:Vmn2r58	APN	7	41,514,703 (GRCm39)	missense	probably damaging	0.97
IGL02222:Vmn2r58	APN	7	41,513,449 (GRCm39)	missense	possibly damaging	0.81
IGL02317:Vmn2r58	APN	7	41,486,765 (GRCm39)	missense	possibly damaging	0.89
IGL02614:Vmn2r58	APN	7	41,486,553 (GRCm39)	missense	probably damaging	1.00
IGL02673:Vmn2r58	APN	7	41,514,082 (GRCm39)	missense	possibly damaging	0.90
IGL03323:Vmn2r58	APN	7	41,511,295 (GRCm39)	missense	probably benign	0.06
IGL03337:Vmn2r58	APN	7	41,513,810 (GRCm39)	missense	possibly damaging	0.93
IGL03380:Vmn2r58	APN	7	41,513,874 (GRCm39)	missense	probably benign	0.00
ANU05:Vmn2r58	UTSW	7	41,513,935 (GRCm39)	missense	probably benign	0.02
R0138:Vmn2r58	UTSW	7	41,487,048 (GRCm39)	missense	probably damaging	1.00
R0141:Vmn2r58	UTSW	7	41,511,309 (GRCm39)	missense	probably benign	0.11
R0421:Vmn2r58	UTSW	7	41,514,628 (GRCm39)	missense	probably benign	0.02
R0604:Vmn2r58	UTSW	7	41,510,000 (GRCm39)	missense	possibly damaging	0.78
R0854:Vmn2r58	UTSW	7	41,486,562 (GRCm39)	missense	probably damaging	1.00
R1413:Vmn2r58	UTSW	7	41,513,387 (GRCm39)	missense	probably benign	0.01
R1441:Vmn2r58	UTSW	7	41,486,864 (GRCm39)	missense	probably damaging	1.00
R1678:Vmn2r58	UTSW	7	41,513,480 (GRCm39)	missense	probably benign	0.40
R1691:Vmn2r58	UTSW	7	41,486,913 (GRCm39)	missense	possibly damaging	0.95
R1699:Vmn2r58	UTSW	7	41,509,951 (GRCm39)	missense	probably benign
R1865:Vmn2r58	UTSW	7	41,486,682 (GRCm39)	missense	possibly damaging	0.95
R2008:Vmn2r58	UTSW	7	41,509,924 (GRCm39)	missense	probably damaging	1.00
R2036:Vmn2r58	UTSW	7	41,513,417 (GRCm39)	missense	probably benign
R2202:Vmn2r58	UTSW	7	41,513,594 (GRCm39)	missense	probably benign	0.07
R3787:Vmn2r58	UTSW	7	41,513,498 (GRCm39)	missense	probably benign	0.01
R3883:Vmn2r58	UTSW	7	41,513,914 (GRCm39)	nonsense	probably null
R3944:Vmn2r58	UTSW	7	41,513,885 (GRCm39)	missense	probably benign	0.03
R3949:Vmn2r58	UTSW	7	41,513,348 (GRCm39)	missense	probably benign	0.08
R4232:Vmn2r58	UTSW	7	41,487,011 (GRCm39)	missense	possibly damaging	0.91
R4409:Vmn2r58	UTSW	7	41,522,051 (GRCm39)	missense	possibly damaging	0.69
R4411:Vmn2r58	UTSW	7	41,511,360 (GRCm39)	missense	possibly damaging	0.85
R4413:Vmn2r58	UTSW	7	41,511,360 (GRCm39)	missense	possibly damaging	0.85
R4600:Vmn2r58	UTSW	7	41,522,046 (GRCm39)	missense	probably benign	0.03
R4610:Vmn2r58	UTSW	7	41,487,117 (GRCm39)	missense	probably benign
R4646:Vmn2r58	UTSW	7	41,509,935 (GRCm39)	missense	probably damaging	0.96
R4793:Vmn2r58	UTSW	7	41,514,495 (GRCm39)	missense	probably damaging	0.99
R4870:Vmn2r58	UTSW	7	41,486,639 (GRCm39)	missense	possibly damaging	0.76
R4981:Vmn2r58	UTSW	7	41,486,885 (GRCm39)	missense	probably damaging	1.00
R4993:Vmn2r58	UTSW	7	41,487,176 (GRCm39)	missense	probably benign	0.00
R5024:Vmn2r58	UTSW	7	41,513,746 (GRCm39)	missense	probably damaging	0.99
R5064:Vmn2r58	UTSW	7	41,486,534 (GRCm39)	missense	probably damaging	0.99
R5330:Vmn2r58	UTSW	7	41,513,384 (GRCm39)	nonsense	probably null
R5526:Vmn2r58	UTSW	7	41,522,069 (GRCm39)	missense	probably benign	0.01
R5980:Vmn2r58	UTSW	7	41,514,480 (GRCm39)	missense	possibly damaging	0.81
R6163:Vmn2r58	UTSW	7	41,486,825 (GRCm39)	missense	probably benign	0.31
R6365:Vmn2r58	UTSW	7	41,513,607 (GRCm39)	missense	probably benign	0.42
R6594:Vmn2r58	UTSW	7	41,486,535 (GRCm39)	missense	possibly damaging	0.69
R6980:Vmn2r58	UTSW	7	41,513,662 (GRCm39)	missense	possibly damaging	0.64
R7373:Vmn2r58	UTSW	7	41,487,212 (GRCm39)	missense	probably damaging	1.00
R7458:Vmn2r58	UTSW	7	41,487,123 (GRCm39)	missense	probably benign	0.06
R7630:Vmn2r58	UTSW	7	41,513,611 (GRCm39)	missense	probably damaging	0.99
R7807:Vmn2r58	UTSW	7	41,521,910 (GRCm39)	missense	probably benign	0.05
R8114:Vmn2r58	UTSW	7	41,511,392 (GRCm39)	missense	probably damaging	1.00
R8232:Vmn2r58	UTSW	7	41,514,076 (GRCm39)	missense	probably damaging	0.97
R8313:Vmn2r58	UTSW	7	41,521,952 (GRCm39)	missense	probably benign	0.01
R8412:Vmn2r58	UTSW	7	41,513,722 (GRCm39)	missense	probably benign	0.01
R8530:Vmn2r58	UTSW	7	41,513,576 (GRCm39)	missense	probably damaging	1.00
R8851:Vmn2r58	UTSW	7	41,487,219 (GRCm39)	missense	probably benign
R8881:Vmn2r58	UTSW	7	41,486,609 (GRCm39)	missense	probably benign	0.05
R8936:Vmn2r58	UTSW	7	41,513,981 (GRCm39)	missense
R9045:Vmn2r58	UTSW	7	41,487,087 (GRCm39)	missense	probably benign	0.00
R9166:Vmn2r58	UTSW	7	41,513,431 (GRCm39)	missense	probably damaging	1.00
R9706:Vmn2r58	UTSW	7	41,510,000 (GRCm39)	missense	probably damaging	0.99
RF006:Vmn2r58	UTSW	7	41,486,383 (GRCm39)	frame shift	probably null
RF027:Vmn2r58	UTSW	7	41,486,383 (GRCm39)	frame shift	probably null
Z1176:Vmn2r58	UTSW	7	41,513,789 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CAATGTGGGCAGATGCTGAC -3'
(R):5'- CTGGAGTCCAAGAGATAATACTTAGTG -3'

Sequencing Primer
(F):5'- CCATGATGGTCCTGTGAGTACAAC -3'
(R):5'- TCTCATCTGCTTAAAACACACATTC -3'

Posted On

2018-06-06